LOCUS       Exported     3052 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..3052
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      1090..1137
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     2985..3001
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      3002..3033
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             29..1018
                     /label="pstB"
                     /note="Part of the ABC transporter complex PstSACB involved in phosphate import. Responsible for energy coupling to the transport system."
                     /translation="MKKGLKTIWHNFIQKREKVKQYRALYEKQIKQYQQKVAKLDPTTKAEEIANLQSEIDVLQRLIKIKNTKDDVVKQDFDKKNVFEIENLNFWYNKDKQVLFDINLKIKRNKITALIGKSGCGKSTFIRCLNKLNDLNENVRWNGKIFFLGKNINSGIINDLTLRTRVGMVFQQLTPFNFSIFENIAYGLRAHGIHNKQAIHEIVEQALKSTALWDEVKDNLHRNANTLSGGQQQRLCIARAIALQPDVLLMDEPTSALDSIATNSIELLIQQLKEKYTIIIVTHSMAQTIRITDETIFFANGRVVEQGTTKQIFTRPKQKETNRYISGRN"
     primer_bind     complement(1142..1158)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(1225..1813)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(2004..2864)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(2865..2969)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
ORIGIN
        1 ACAAGGAGTC GGCATATCAC GGTCTCAAAT GAAAAAGGGC CTCAAGACGA TCTGGCATAA
       61 CTTTATTCAA AAGCGTGAGA AAGTCAAACA ATATCGTGCG CTGTACGAGA AGCAGATCAA
      121 ACAGTATCAA CAAAAGGTCG CGAAGCTGGA CCCAACCACT AAGGCGGAAG AGATCGCAAA
      181 TCTGCAGTCC GAAATCGACG TGCTGCAGCG TCTGATTAAG ATCAAAAATA CCAAGGATGA
      241 CGTGGTCAAA CAAGACTTTG ATAAAAAGAA TGTGTTCGAG ATTGAGAATC TGAACTTCTG
      301 GTACAATAAA GATAAGCAAG TTTTGTTCGA TATTAACCTC AAGATTAAGC GCAATAAAAT
      361 CACCGCCTTG ATCGGTAAGA GCGGCTGCGG CAAAAGCACG TTCATCCGCT GCTTGAATAA
      421 ACTGAATGAT CTGAACGAAA ATGTTCGCTG GAACGGTAAG ATTTTCTTCC TGGGCAAGAA
      481 CATTAATAGC GGCATTATCA ATGATCTGAC GCTGCGCACG CGTGTTGGCA TGGTCTTTCA
      541 GCAACTGACG CCATTTAATT TTAGCATTTT TGAGAATATC GCTTATGGTT TGCGTGCGCA
      601 CGGCATCCAC AACAAGCAAG CGATCCATGA AATTGTCGAG CAGGCACTCA AGAGCACCGC
      661 TCTGTGGGAT GAGGTGAAAG ACAATCTGCA CCGTAACGCA AACACGCTGT CGGGCGGCCA
      721 GCAGCAACGT CTGTGCATTG CACGTGCGAT CGCTCTGCAG CCGGATGTCC TGCTGATGGA
      781 CGAACCGACC TCTGCACTGG ACTCGATCGC GACCAACAGC ATTGAACTGC TGATTCAGCA
      841 ACTGAAGGAA AAGTATACCA TTATTATTGT CACCCATAGC ATGGCCCAAA CCATCCGTAT
      901 CACCGACGAA ACGATCTTTT TTGCGAATGG TCGTGTTGTG GAGCAGGGCA CCACCAAACA
      961 GATTTTTACG CGTCCGAAAC AGAAGGAAAC CAATCGCTAC ATTAGCGGTC GTAACTGAAG
     1021 AGCTTAGAGA CCGCGGGTTA GATACTGCAA CTCGCTGAGG TGTCAATCGT CGGAGCCGCT
     1081 GAGCAATAAC TAGCATAACC CCTTGGGGCC TCTAAACGGG TCTTGAGGGG TTTTTTGCAT
     1141 GGTCATAGCT GTTTCCTGAG AGCTTGGCAG GTGATGACAC ACATTAACAA ATTTCGTGAG
     1201 GAGTCTCCAG AAGAATGCCA TTAATTTCCA TAGGCTCCGC CCCCCTGACG AGCATCACAA
     1261 AAATCGACGC TCAAGTCAGA GGTGGCGAAA CCCGACAGGA CTATAAAGAT ACCAGGCGTT
     1321 TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC TGTTCCGACC CTGCCGCTTA CCGGATACCT
     1381 GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC GCTTTCTCAT AGCTCACGCT GTAGGTATCT
     1441 CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT GGGCTGTGTG CACGAACCCC CCGTTCAGCC
     1501 CGACCGCTGC GCCTTATCCG GTAACTATCG TCTTGAGTCC AACCCGGTAA GACACGACTT
     1561 ATCGCCACTG GCAGCAGCCA CTGGTAACAG GATTAGCAGA GCGAGGTATG TAGGCGGTGC
     1621 TACAGAGTTC TTGAAGTGGT GGCCTAACTA CGGCTACACT AGAAGAACAG TATTTGGTAT
     1681 CTGCGCTCTG CTGAAGCCAG TTACCTTCGG AAAAAGAGTT GGTAGCTCTT GATCCGGCAA
     1741 ACAAACCACC GCTGGTAGCG GTGGTTTTTT TGTTTGCAAG CAGCAGATTA CGCGCAGAAA
     1801 AAAAGGATCT CAAGAAGGCC TACTATTAGC AACAACGATC CTTTGATCTT TTCTACGGGG
     1861 TCTGACGCTC AGTGGAACGA AAACTCACGT TAAGGGATTT TGGTCATGAG ATTATCAAAA
     1921 AGGATCTTCA CCTAGATCCT TTTAAATTAA AAATGAAGTT TTAAATCAAT CTAAAGTATA
     1981 TATGAGTAAA CTTGGTCTGA CAGTTACCAA TGCTTAATCA GTGAGGCACC TATCTCAGCG
     2041 ATCTGTCTAT TTCGTTCATC CATAGTTGCC TGACTCCCCG TCGTGTAGAT AACTACGATA
     2101 CGGGAGGGCT TACCATCTGG CCCCAGTGCT GCAATGATAC CGCGAGAACC ACGCTCACCG
     2161 GCTCCAGATT TATCAGCAAT AAACCAGCCA GCCGGAAGGG CCGAGCGCAG AAGTGGTCCT
     2221 GCAACTTTAT CCGCCTCCAT CCAGTCTATT AATTGTTGCC GGGAAGCTAG AGTAAGTAGT
     2281 TCGCCAGTTA ATAGTTTGCG CAACGTTGTT GCCATTGCTA CAGGCATCGT GGTGTCACGC
     2341 TCGTCGTTTG GTATGGCTTC ATTCAGCTCC GGTTCCCAAC GATCAAGGCG AGTTACATGA
     2401 TCCCCCATGT TGTGCAAAAA AGCGGTTAGC TCCTTCGGTC CTCCGATCGT TGTCAGAAGT
     2461 AAGTTGGCCG CAGTGTTATC ACTCATGGTT ATGGCAGCAC TGCATAATTC TCTTACTGTC
     2521 ATGCCATCCG TAAGATGCTT TTCTGTGACT GGTGAGTACT CAACCAAGTC ATTCTGAGAA
     2581 TAGTGTATGC GGCGACCGAG TTGCTCTTGC CCGGCGTCAA TACGGGATAA TACCGCGCCA
     2641 CATAGCAGAA CTTTAAAAGT GCTCATCATT GGAAAACGTT CTTCGGGGCG AAAACTCTCA
     2701 AGGATCTTAC CGCTGTTGAG ATCCAGTTCG ATGTAACCCA CTCGTGCACC CAACTGATCT
     2761 TCAGCATCTT TTACTTTCAC CAGCGTTTCT GGGTGAGCAA AAACAGGAAG GCAAAATGCC
     2821 GCAAAAAAGG GAATAAGGGC GACACGGAAA TGTTGAATAC TCATACTCTT CCTTTTTCAA
     2881 TATTATTGAA GCATTTATCA GGGTTATTGT CTCATGAGCG GATACATATT TGAATGTATT
     2941 TAGAAAAATA AACAAATAGG GGTTCCGCGC ACCTGCACCA GTCAGTAAAA CGACGGCCAG
     3001 TAGTCAAAAG CCTCCGACCG GAGGCTTTTG ACTTGGTCAG GTGGAGTGGG AG
//