LOCUS       Exported     3097 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..3097
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      1135..1182
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     3030..3046
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      3047..3078
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             29..1063
                     /label="MPN613"
                     /note=""
                     /translation="MVFSFQDDTALTVALNSIKLVKNPEELIPGLIPTNPKTGIFYIAAEITNSNNFFKWGTDSNREIIKGQMYFLKKETKIPTIRTYLFQMRTHKIALNANDVWFQANNDKVKVIVDGIELDQFDPKLPNVAFFNDYQVDLVSTLTLADKQLLIRRLNLALVGMNLMVDEQATNIETFPKQIRLTTKSIDQSFDFDVEFNPKASELNVIVKVDEQPLFKLDYELVIKNSNTLQLVNRNKHLGMYIWSVADQRYQAKLLDTLQLFLQSKQMYLKEKIPLKLQDNTATFKIAKQAQPNNGENKNTNFIGYLVIAASSLFLVVVAFSFYFYKRKKNLKSHKQKAVVKTDK"
     primer_bind     complement(1187..1203)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(1270..1858)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(2049..2909)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(2910..3014)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
ORIGIN
        1 AAGGACTGCA TACCAGGTTG GGTCTCAAAT GGTATTTAGC TTCCAAGATG ATACTGCACT
       61 GACGGTTGCT CTGAATAGCA TCAAACTGGT TAAGAATCCG GAGGAGCTGA TCCCGGGCCT
      121 GATCCCGACC AACCCGAAAA CGGGTATTTT CTATATCGCG GCGGAAATTA CCAACAGCAA
      181 TAACTTTTTT AAATGGGGTA CCGATAGCAA CCGTGAGATC ATTAAGGGCC AGATGTATTT
      241 TTTGAAGAAG GAAACCAAAA TCCCTACCAT CCGCACCTAC CTGTTTCAGA TGCGTACCCA
      301 CAAAATTGCG CTGAACGCTA ACGACGTGTG GTTCCAGGCG AACAACGACA AGGTTAAAGT
      361 GATTGTCGAT GGCATTGAAC TGGATCAGTT CGACCCAAAG CTGCCGAACG TGGCGTTCTT
      421 CAACGACTAT CAAGTGGACC TGGTGTCTAC CCTGACCCTG GCCGATAAGC AATTGCTGAT
      481 TCGCCGCCTG AACCTGGCAC TGGTCGGCAT GAATCTGATG GTCGACGAAC AGGCAACGAA
      541 CATTGAAACC TTTCCGAAGC AGATTCGCCT GACTACCAAG AGCATTGATC AGAGCTTTGA
      601 CTTCGATGTT GAGTTCAACC CGAAGGCCAG CGAGCTGAAC GTGATCGTTA AGGTGGATGA
      661 ACAACCGCTG TTTAAGCTGG ATTATGAGCT GGTTATCAAG AATAGCAACA CCTTACAGCT
      721 GGTGAATAGA AATAAGCACT TAGGCATGTA TATCTGGAGC GTTGCAGATC AGCGTTATCA
      781 AGCGAAATTG CTGGACACCC TGCAGCTGTT CCTGCAATCG AAACAAATGT ACCTCAAGGA
      841 AAAGATCCCG CTGAAGCTGC AGGATAACAC CGCGACCTTT AAAATCGCCA AACAGGCGCA
      901 GCCAAACAAT GGTGAGAATA AAAACACCAA CTTCATCGGC TATTTGGTGA TCGCCGCGTC
      961 TAGCCTGTTT CTGGTGGTTG TTGCATTTAG CTTTTATTTT TATAAGCGCA AAAAGAATTT
     1021 AAAGAGCCAC AAGCAAAAGG CGGTTGTCAA GACCGATAAG TGAAGAGCTT AGAGACCGTT
     1081 GTATCCTTCT GGGCCTTCGC TGAGGTGTCA ATCGTCGGAG CCGCTGAGCA ATAACTAGCA
     1141 TAACCCCTTG GGGCCTCTAA ACGGGTCTTG AGGGGTTTTT TGCATGGTCA TAGCTGTTTC
     1201 CTGAGAGCTT GGCAGGTGAT GACACACATT AACAAATTTC GTGAGGAGTC TCCAGAAGAA
     1261 TGCCATTAAT TTCCATAGGC TCCGCCCCCC TGACGAGCAT CACAAAAATC GACGCTCAAG
     1321 TCAGAGGTGG CGAAACCCGA CAGGACTATA AAGATACCAG GCGTTTCCCC CTGGAAGCTC
     1381 CCTCGTGCGC TCTCCTGTTC CGACCCTGCC GCTTACCGGA TACCTGTCCG CCTTTCTCCC
     1441 TTCGGGAAGC GTGGCGCTTT CTCATAGCTC ACGCTGTAGG TATCTCAGTT CGGTGTAGGT
     1501 CGTTCGCTCC AAGCTGGGCT GTGTGCACGA ACCCCCCGTT CAGCCCGACC GCTGCGCCTT
     1561 ATCCGGTAAC TATCGTCTTG AGTCCAACCC GGTAAGACAC GACTTATCGC CACTGGCAGC
     1621 AGCCACTGGT AACAGGATTA GCAGAGCGAG GTATGTAGGC GGTGCTACAG AGTTCTTGAA
     1681 GTGGTGGCCT AACTACGGCT ACACTAGAAG AACAGTATTT GGTATCTGCG CTCTGCTGAA
     1741 GCCAGTTACC TTCGGAAAAA GAGTTGGTAG CTCTTGATCC GGCAAACAAA CCACCGCTGG
     1801 TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA GATTACGCGC AGAAAAAAAG GATCTCAAGA
     1861 AGGCCTACTA TTAGCAACAA CGATCCTTTG ATCTTTTCTA CGGGGTCTGA CGCTCAGTGG
     1921 AACGAAAACT CACGTTAAGG GATTTTGGTC ATGAGATTAT CAAAAAGGAT CTTCACCTAG
     1981 ATCCTTTTAA ATTAAAAATG AAGTTTTAAA TCAATCTAAA GTATATATGA GTAAACTTGG
     2041 TCTGACAGTT ACCAATGCTT AATCAGTGAG GCACCTATCT CAGCGATCTG TCTATTTCGT
     2101 TCATCCATAG TTGCCTGACT CCCCGTCGTG TAGATAACTA CGATACGGGA GGGCTTACCA
     2161 TCTGGCCCCA GTGCTGCAAT GATACCGCGA GAACCACGCT CACCGGCTCC AGATTTATCA
     2221 GCAATAAACC AGCCAGCCGG AAGGGCCGAG CGCAGAAGTG GTCCTGCAAC TTTATCCGCC
     2281 TCCATCCAGT CTATTAATTG TTGCCGGGAA GCTAGAGTAA GTAGTTCGCC AGTTAATAGT
     2341 TTGCGCAACG TTGTTGCCAT TGCTACAGGC ATCGTGGTGT CACGCTCGTC GTTTGGTATG
     2401 GCTTCATTCA GCTCCGGTTC CCAACGATCA AGGCGAGTTA CATGATCCCC CATGTTGTGC
     2461 AAAAAAGCGG TTAGCTCCTT CGGTCCTCCG ATCGTTGTCA GAAGTAAGTT GGCCGCAGTG
     2521 TTATCACTCA TGGTTATGGC AGCACTGCAT AATTCTCTTA CTGTCATGCC ATCCGTAAGA
     2581 TGCTTTTCTG TGACTGGTGA GTACTCAACC AAGTCATTCT GAGAATAGTG TATGCGGCGA
     2641 CCGAGTTGCT CTTGCCCGGC GTCAATACGG GATAATACCG CGCCACATAG CAGAACTTTA
     2701 AAAGTGCTCA TCATTGGAAA ACGTTCTTCG GGGCGAAAAC TCTCAAGGAT CTTACCGCTG
     2761 TTGAGATCCA GTTCGATGTA ACCCACTCGT GCACCCAACT GATCTTCAGC ATCTTTTACT
     2821 TTCACCAGCG TTTCTGGGTG AGCAAAAACA GGAAGGCAAA ATGCCGCAAA AAAGGGAATA
     2881 AGGGCGACAC GGAAATGTTG AATACTCATA CTCTTCCTTT TTCAATATTA TTGAAGCATT
     2941 TATCAGGGTT ATTGTCTCAT GAGCGGATAC ATATTTGAAT GTATTTAGAA AAATAAACAA
     3001 ATAGGGGTTC CGCGCACCTG CACCAGTCAG TAAAACGACG GCCAGTAGTC AAAAGCCTCC
     3061 GACCGGAGGC TTTTGACTTG GTCAGGTGGA GTGGGAG
//