LOCUS       Exported     3781 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..3781
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
                     /label="T7 terminator"
     terminator      1819..1866
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     3714..3730
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      3731..3762
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             29..1747
                     /label="ptsI"
                     /note="General (non sugar-specific) component of the phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS). This major carbohydrate active-transport system catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. Enzyme I transfers the phosphoryl group from phosphoenolpyruvate (PEP) to the phosphoryl carrier protein (HPr)."
                     /translation="MKKLSGIGVSDGMALAKAFLVKTPEFAVNKYLKHQLTKAQAKRLLDSAFKKAVKDLEEIKEITVNNINTEAGMIFDAHIQMLNDPTITEQLEQQLAQNVHPVIAVDTVFSQTATMFSQMQDKYFQERAADILDLRQRLLAYLTGQKPHDLVKIKSDVIIVAHDLTPSQTATLNKKYVKGFLTEIGGRTSHAAIMARSLEIPAVVGIKGITTKVKDGQIVGVDGRKGIAGLDLNSKDTTEWKKQKALEEKYQQELKQYTNKETVTLDGHAVVVAANIGNVKDMELACQYNTNGVGLFRTEFLYMNSQEWPDEETQYQAYKAVLEQAHGDLVIIRTLDIGGDKKLNYYEFPHEDNPFLGYRALRLTLDKQDIFKTQLRALLRAADHGQLGIMFPMVATLDELLQAKQLLNQVHQELGGNKQFKLGIMIEIPAAVLAANTLSHHVDFFSIGTNDLIQYSFAADRMNKNVSYLYQPLNPALLKLIYLTIEGGKVNDIWTGMCGEMAGEPLAIPLLLGLGLKEFSMSASSMFKARMIIAKLNYTECQTLAQKALTLANAKEVEKLVEKFFKKKDIFI"
     primer_bind     complement(1871..1887)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(1954..2542)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(2733..3593)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(3594..3698)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
ORIGIN
        1 AAATAAGACG ACGACCCTCG GGTCTCAAAT GAAGAAACTG AGCGGCATCG GCGTTAGCGA
       61 CGGCATGGCA TTGGCAAAAG CGTTCCTGGT CAAGACCCCG GAGTTCGCGG TCAATAAATA
      121 CCTGAAGCAC CAACTGACGA AAGCACAAGC AAAACGCCTG TTGGACTCAG CATTCAAGAA
      181 GGCGGTGAAA GACCTGGAGG AAATTAAGGA GATCACCGTC AACAATATTA ATACCGAGGC
      241 CGGTATGATC TTCGATGCAC ATATCCAAAT GCTGAACGAC CCGACCATTA CCGAGCAGCT
      301 GGAACAGCAA CTGGCCCAAA ATGTCCATCC GGTTATCGCA GTAGACACGG TGTTTAGCCA
      361 GACGGCCACC ATGTTCAGCC AGATGCAGGA CAAGTACTTT CAGGAGCGTG CAGCAGATAT
      421 TTTGGATCTG CGTCAGCGCC TGCTGGCTTA TCTGACCGGT CAAAAGCCAC ACGACCTGGT
      481 GAAGATTAAG TCGGATGTTA TCATTGTTGC GCATGACTTG ACCCCGTCGC AGACCGCGAC
      541 CCTGAACAAA AAATACGTAA AGGGCTTCCT GACCGAAATC GGCGGCCGTA CGTCCCATGC
      601 CGCGATCATG GCTCGTAGCC TGGAAATTCC GGCCGTGGTC GGTATTAAAG GTATCACGAC
      661 GAAGGTGAAG GATGGCCAAA TTGTTGGCGT TGATGGCCGT AAGGGCATTG CTGGTCTGGA
      721 CTTGAACAGC AAGGATACTA CCGAGTGGAA GAAGCAAAAG GCGCTGGAGG AAAAGTATCA
      781 GCAAGAGCTG AAACAGTATA CGAATAAGGA AACGGTGACG CTGGATGGCC ACGCGGTTGT
      841 TGTGGCAGCC AATATTGGCA ATGTTAAGGA CATGGAACTG GCGTGCCAGT ATAATACCAA
      901 CGGCGTCGGC CTGTTCCGTA CCGAATTTCT GTACATGAAT AGCCAAGAGT GGCCGGATGA
      961 GGAGACTCAA TACCAGGCGT ACAAAGCAGT GCTGGAACAA GCGCACGGCG ATCTGGTTAT
     1021 TATTCGTACC CTGGATATTG GCGGTGATAA GAAGCTGAAC TACTACGAGT TCCCGCATGA
     1081 GGACAACCCG TTCCTGGGCT ACCGCGCACT GCGTTTGACC CTGGACAAAC AGGATATTTT
     1141 TAAGACCCAG CTGCGCGCTC TGCTGCGTGC GGCAGACCAC GGTCAACTGG GTATCATGTT
     1201 TCCGATGGTG GCGACCCTGG ACGAGCTGCT GCAGGCGAAG CAGCTGCTGA ACCAGGTCCA
     1261 CCAGGAACTG GGTGGTAATA AACAGTTCAA ACTGGGTATC ATGATCGAGA TCCCGGCGGC
     1321 AGTACTGGCA GCAAATACCC TGAGCCACCA TGTTGATTTC TTCAGCATCG GTACCAATGA
     1381 TCTGATCCAA TACTCCTTCG CGGCTGACCG CATGAACAAG AATGTGAGCT ATCTGTATCA
     1441 ACCGCTGAAT CCGGCTTTGT TGAAGCTGAT TTATCTGACC ATTGAGGGCG GTAAGGTGAA
     1501 CGACATTTGG ACGGGTATGT GCGGTGAGAT GGCCGGCGAA CCGCTGGCCA TCCCGTTGCT
     1561 CTTAGGCTTG GGTCTGAAAG AATTTAGCAT GAGCGCGTCC AGCATGTTCA AAGCCCGCAT
     1621 GATTATTGCG AAGCTGAATT ATACCGAGTG TCAAACGCTG GCGCAAAAGG CTTTAACGCT
     1681 GGCCAACGCA AAGGAGGTTG AGAAGTTGGT CGAGAAATTT TTCAAGAAAA AAGACATCTT
     1741 CATTTGAAGA GCTTAGAGAC CGTAGTGCCT GTTCGTTCTG TCGCTGAGGT GTCAATCGTC
     1801 GGAGCCGCTG AGCAATAACT AGCATAACCC CTTGGGGCCT CTAAACGGGT CTTGAGGGGT
     1861 TTTTTGCATG GTCATAGCTG TTTCCTGAGA GCTTGGCAGG TGATGACACA CATTAACAAA
     1921 TTTCGTGAGG AGTCTCCAGA AGAATGCCAT TAATTTCCAT AGGCTCCGCC CCCCTGACGA
     1981 GCATCACAAA AATCGACGCT CAAGTCAGAG GTGGCGAAAC CCGACAGGAC TATAAAGATA
     2041 CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT GCGCTCTCCT GTTCCGACCC TGCCGCTTAC
     2101 CGGATACCTG TCCGCCTTTC TCCCTTCGGG AAGCGTGGCG CTTTCTCATA GCTCACGCTG
     2161 TAGGTATCTC AGTTCGGTGT AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC ACGAACCCCC
     2221 CGTTCAGCCC GACCGCTGCG CCTTATCCGG TAACTATCGT CTTGAGTCCA ACCCGGTAAG
     2281 ACACGACTTA TCGCCACTGG CAGCAGCCAC TGGTAACAGG ATTAGCAGAG CGAGGTATGT
     2341 AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG GCCTAACTAC GGCTACACTA GAAGAACAGT
     2401 ATTTGGTATC TGCGCTCTGC TGAAGCCAGT TACCTTCGGA AAAAGAGTTG GTAGCTCTTG
     2461 ATCCGGCAAA CAAACCACCG CTGGTAGCGG TGGTTTTTTT GTTTGCAAGC AGCAGATTAC
     2521 GCGCAGAAAA AAAGGATCTC AAGAAGGCCT ACTATTAGCA ACAACGATCC TTTGATCTTT
     2581 TCTACGGGGT CTGACGCTCA GTGGAACGAA AACTCACGTT AAGGGATTTT GGTCATGAGA
     2641 TTATCAAAAA GGATCTTCAC CTAGATCCTT TTAAATTAAA AATGAAGTTT TAAATCAATC
     2701 TAAAGTATAT ATGAGTAAAC TTGGTCTGAC AGTTACCAAT GCTTAATCAG TGAGGCACCT
     2761 ATCTCAGCGA TCTGTCTATT TCGTTCATCC ATAGTTGCCT GACTCCCCGT CGTGTAGATA
     2821 ACTACGATAC GGGAGGGCTT ACCATCTGGC CCCAGTGCTG CAATGATACC GCGAGAACCA
     2881 CGCTCACCGG CTCCAGATTT ATCAGCAATA AACCAGCCAG CCGGAAGGGC CGAGCGCAGA
     2941 AGTGGTCCTG CAACTTTATC CGCCTCCATC CAGTCTATTA ATTGTTGCCG GGAAGCTAGA
     3001 GTAAGTAGTT CGCCAGTTAA TAGTTTGCGC AACGTTGTTG CCATTGCTAC AGGCATCGTG
     3061 GTGTCACGCT CGTCGTTTGG TATGGCTTCA TTCAGCTCCG GTTCCCAACG ATCAAGGCGA
     3121 GTTACATGAT CCCCCATGTT GTGCAAAAAA GCGGTTAGCT CCTTCGGTCC TCCGATCGTT
     3181 GTCAGAAGTA AGTTGGCCGC AGTGTTATCA CTCATGGTTA TGGCAGCACT GCATAATTCT
     3241 CTTACTGTCA TGCCATCCGT AAGATGCTTT TCTGTGACTG GTGAGTACTC AACCAAGTCA
     3301 TTCTGAGAAT AGTGTATGCG GCGACCGAGT TGCTCTTGCC CGGCGTCAAT ACGGGATAAT
     3361 ACCGCGCCAC ATAGCAGAAC TTTAAAAGTG CTCATCATTG GAAAACGTTC TTCGGGGCGA
     3421 AAACTCTCAA GGATCTTACC GCTGTTGAGA TCCAGTTCGA TGTAACCCAC TCGTGCACCC
     3481 AACTGATCTT CAGCATCTTT TACTTTCACC AGCGTTTCTG GGTGAGCAAA AACAGGAAGG
     3541 CAAAATGCCG CAAAAAAGGG AATAAGGGCG ACACGGAAAT GTTGAATACT CATACTCTTC
     3601 CTTTTTCAAT ATTATTGAAG CATTTATCAG GGTTATTGTC TCATGAGCGG ATACATATTT
     3661 GAATGTATTT AGAAAAATAA ACAAATAGGG GTTCCGCGCA CCTGCACCAG TCAGTAAAAC
     3721 GACGGCCAGT AGTCAAAAGC CTCCGACCGG AGGCTTTTGA CTTGGTCAGG TGGAGTGGGA
     3781 G
//