LOCUS       Exported     3190 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..3190
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      1228..1275
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     3123..3139
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      3140..3171
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             29..1156
                     /label="MPN638"
                     /note="The M and S subunits together form a methyltransferase (MTase) that methylates two adenine residues in complementary strands of a bipartite DNA recognition sequence. Subunit S dictates DNA sequences specificity (By similarity)."
                     /translation="MTPKLKLNTNSNWTKKTLGSLFELKKGEMLEKELLAPDGKYEYFNGGIKASGRTNEFNTFKNTISIIIGGSCGYVRLADKDYFCGQSSCTLTVLDPLEIDLKFAYYALKSQEEKITSLASGTTIKNIRLSDLKDLPIPLVKSIQDQRTIAHALSVFDLRIEHLNELIEVNRKLRDEYAHKLFTLDPDFLTHWNLHELHEQMGEISLGEVFHLKSGKYLKADERFEDGKFPYYGAGIESTSFVNEPNTKGDTLSMIANGYSIGNIRYHTIPWFNGTGGIAMEALKPNKTYVPFFYCALKYMQKDLKERFKRDESPFISLKLAGEIKVPFVKSFALQRKAGKIIYLLDKTLEECKEEAKSLISIRDNLLGKLFPTLS"
     primer_bind     complement(1280..1296)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(1363..1951)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(2142..3002)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(3003..3107)
                     /note="ampicillin resistance gene promoter"
                     /label="bla promoter"
ORIGIN
        1 AAATAAGACG ACGACCCTCG GGTCTCAAAT GACCCCGAAG CTGAAGCTGA ACACCAATTC
       61 GAACTGGACC AAAAAGACCC TGGGTTCGCT GTTCGAACTG AAGAAGGGCG AGATGTTGGA
      121 GAAGGAGCTT CTGGCACCAG ATGGTAAGTA TGAATATTTC AACGGTGGTA TCAAAGCAAG
      181 CGGCCGCACG AACGAGTTTA ATACCTTTAA GAACACCATC TCTATTATCA TCGGTGGCAG
      241 CTGCGGCTAC GTTCGCCTGG CGGATAAGGA TTACTTCTGT GGCCAATCTA GCTGTACCCT
      301 GACCGTGCTT GACCCGTTGG AGATCGACCT GAAGTTCGCA TACTACGCAC TGAAAAGCCA
      361 AGAGGAGAAG ATTACGAGCC TGGCGTCCGG CACCACGATC AAAAACATTC GCCTGAGCGA
      421 CCTGAAAGAC CTTCCGATCC CGCTGGTCAA GAGCATCCAA GACCAGCGCA CGATCGCGCA
      481 CGCGCTGAGC GTTTTCGATT TGCGCATTGA ACATCTGAAC GAGCTGATCG AAGTGAACCG
      541 TAAACTGCGT GATGAGTACG CGCACAAACT GTTTACCCTG GACCCTGACT TTTTGACTCA
      601 CTGGAATCTG CACGAGCTGC ATGAACAAAT GGGTGAAATT AGCCTGGGCG AAGTTTTTCA
      661 TCTCAAGAGC GGCAAATATT TGAAGGCGGA TGAACGCTTT GAGGATGGTA AGTTCCCGTA
      721 CTACGGTGCA GGCATTGAGA GCACTAGCTT CGTTAACGAA CCGAATACCA AGGGCGATAC
      781 GCTGAGCATG ATCGCAAACG GTTATAGCAT TGGCAACATC CGCTACCATA CCATTCCGTG
      841 GTTCAACGGC ACGGGTGGCA TTGCCATGGA GGCACTGAAA CCGAATAAAA CCTACGTGCC
      901 TTTTTTCTAT TGTGCACTGA AATACATGCA AAAGGACCTG AAGGAGCGCT TCAAGCGTGA
      961 TGAGAGCCCG TTTATTAGCC TGAAGCTGGC CGGTGAGATC AAGGTTCCGT TTGTCAAGTC
     1021 TTTCGCTCTG CAGCGCAAGG CTGGCAAGAT CATTTACTTG CTGGACAAGA CGCTGGAGGA
     1081 GTGCAAAGAG GAAGCCAAAA GCCTGATCTC TATCCGTGAT AATTTACTGG GCAAGCTGTT
     1141 CCCGACCTTA TCCTGAAGAG CTTAGAGACC GGTACGGTTT ACAGACCACT CGCTGAGGTG
     1201 TCAATCGTCG GAGCCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC TAAACGGGTC
     1261 TTGAGGGGTT TTTTGCATGG TCATAGCTGT TTCCTGAGAG CTTGGCAGGT GATGACACAC
     1321 ATTAACAAAT TTCGTGAGGA GTCTCCAGAA GAATGCCATT AATTTCCATA GGCTCCGCCC
     1381 CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC CGACAGGACT
     1441 ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG TTCCGACCCT
     1501 GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC TTTCTCATAG
     1561 CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA
     1621 CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC TTGAGTCCAA
     1681 CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA TTAGCAGAGC
     1741 GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG GCTACACTAG
     1801 AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG
     1861 TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA
     1921 GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGGCCTA CTATTAGCAA CAACGATCCT
     1981 TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA AGGGATTTTG
     2041 GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA ATGAAGTTTT
     2101 AAATCAATCT AAAGTATATA TGAGTAAACT TGGTCTGACA GTTACCAATG CTTAATCAGT
     2161 GAGGCACCTA TCTCAGCGAT CTGTCTATTT CGTTCATCCA TAGTTGCCTG ACTCCCCGTC
     2221 GTGTAGATAA CTACGATACG GGAGGGCTTA CCATCTGGCC CCAGTGCTGC AATGATACCG
     2281 CGAGAACCAC GCTCACCGGC TCCAGATTTA TCAGCAATAA ACCAGCCAGC CGGAAGGGCC
     2341 GAGCGCAGAA GTGGTCCTGC AACTTTATCC GCCTCCATCC AGTCTATTAA TTGTTGCCGG
     2401 GAAGCTAGAG TAAGTAGTTC GCCAGTTAAT AGTTTGCGCA ACGTTGTTGC CATTGCTACA
     2461 GGCATCGTGG TGTCACGCTC GTCGTTTGGT ATGGCTTCAT TCAGCTCCGG TTCCCAACGA
     2521 TCAAGGCGAG TTACATGATC CCCCATGTTG TGCAAAAAAG CGGTTAGCTC CTTCGGTCCT
     2581 CCGATCGTTG TCAGAAGTAA GTTGGCCGCA GTGTTATCAC TCATGGTTAT GGCAGCACTG
     2641 CATAATTCTC TTACTGTCAT GCCATCCGTA AGATGCTTTT CTGTGACTGG TGAGTACTCA
     2701 ACCAAGTCAT TCTGAGAATA GTGTATGCGG CGACCGAGTT GCTCTTGCCC GGCGTCAATA
     2761 CGGGATAATA CCGCGCCACA TAGCAGAACT TTAAAAGTGC TCATCATTGG AAAACGTTCT
     2821 TCGGGGCGAA AACTCTCAAG GATCTTACCG CTGTTGAGAT CCAGTTCGAT GTAACCCACT
     2881 CGTGCACCCA ACTGATCTTC AGCATCTTTT ACTTTCACCA GCGTTTCTGG GTGAGCAAAA
     2941 ACAGGAAGGC AAAATGCCGC AAAAAAGGGA ATAAGGGCGA CACGGAAATG TTGAATACTC
     3001 ATACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT CATGAGCGGA
     3061 TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC CTGCACCAGT
     3121 CAGTAAAACG ACGGCCAGTA GTCAAAAGCC TCCGACCGGA GGCTTTTGAC TTGGTCAGGT
     3181 GGAGTGGGAG
//