LOCUS Exported 2893 bp DNA circular SYN 24-JUL-2020 DEFINITION synthetic linear DNA ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2001) AUTHORS Keoni Gandall TITLE Direct Submission JOURNAL Exported from Poly. FEATURES Location/Qualifiers terminator 1..2893 /label="T7 terminator" /note="transcription terminator for bacteriophage T7 RNA polymerase" terminator 931..978 /label="T7 terminator" /note="transcription terminator for bacteriophage T7 RNA polymerase" primer_bind 2826..2842 /label="M13 for" /note="common sequencing primer, one of multiple similar variants" terminator 2843..2874 /label="tonB terminator" /note="bidirectional E. coli tonB-P14 transcription terminator" CDS 29..859 /note="" /translation="MNKKRLLPKASLGALFMLFGTALTACSNSDFQTNLTSLNQLREGVNQNTSLTQDKKAFVESLQKAFENNPEGTTKVLLDAWKFTLLDSKILESKDPSRFVKAFGSGKSNEDVEPNASVKGLRLDKRFEPSTANIINNVISLNEQKVEAFNIQYKSRTSFKVQVKLNAQGKYQKSQVQSYLQQIGLNDGDLKQESGTLSADLIFTYTVPESNLFSRKNFDTLMKKINFNTTLKIDMVGKDEIMKKILQSTTFTNNLSSQTFQDQSIDLLPYLLYSIL" /label="MPN641" primer_bind complement(983..999) /label="M13 rev" /note="common sequencing primer, one of multiple similar variants" rep_origin complement(1066..1654) /label="ori" /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(1845..2705) /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*" /label="bla" promoter complement(2706..2810) /label="bla promoter" /note="ampicillin resistance gene promoter" ORIGIN 1 AAACACGTGG CAAACATTCC GGTCTCAAAT GAATAAGAAA CGTCTGCTGC CTAAGGCGAG 61 CCTCGGCGCG CTGTTCATGC TGTTCGGTAC TGCGCTGACT GCGTGCAGCA ACAGCGATTT 121 TCAGACCAAC CTGACCAGCC TGAATCAACT GCGCGAAGGT GTCAACCAGA ACACCTCTCT 181 GACCCAAGAT AAGAAGGCAT TCGTCGAATC CCTGCAAAAA GCATTCGAGA ACAACCCGGA 241 AGGTACGACC AAGGTGCTCC TGGATGCCTG GAAATTCACC CTGTTAGACA GCAAAATCCT 301 GGAGAGCAAA GATCCGAGCC GCTTTGTTAA AGCGTTCGGC AGCGGTAAGT CTAATGAAGA 361 TGTTGAGCCG AACGCCTCCG TCAAAGGTCT GCGCCTGGAC AAACGTTTCG AACCGAGCAC 421 CGCCAACATT ATCAATAATG TGATTTCCCT GAACGAGCAA AAAGTGGAAG CCTTCAACAT 481 CCAGTACAAA AGCCGCACCA GCTTCAAAGT CCAAGTTAAA CTGAATGCTC AGGGCAAGTA 541 CCAGAAATCT CAAGTCCAAA GCTATCTGCA GCAGATTGGT TTGAACGACG GCGACTTGAA 601 ACAAGAATCT GGCACTCTGT CTGCGGACCT GATTTTCACC TACACGGTGC CGGAAAGCAA 661 CCTCTTCTCG CGCAAAAACT TCGATACCCT GATGAAGAAG ATCAATTTTA ATACTACCCT 721 GAAGATTGAT ATGGTGGGCA AAGATGAAAT CATGAAGAAG ATTCTCCAAA GCACGACCTT 781 CACCAATAAT CTGAGCAGCC AGACCTTTCA AGACCAGAGC ATTGATCTGC TGCCATATCT 841 CCTGTATAGC ATCCTGTGAA GAGCTTAGAG ACCCGCGCTA ATAGCTTTGC ATTCGCTGAG 901 GTGTCAATCG TCGGAGCCGC TGAGCAATAA CTAGCATAAC CCCTTGGGGC CTCTAAACGG 961 GTCTTGAGGG GTTTTTTGCA TGGTCATAGC TGTTTCCTGA GAGCTTGGCA GGTGATGACA 1021 CACATTAACA AATTTCGTGA GGAGTCTCCA GAAGAATGCC ATTAATTTCC ATAGGCTCCG 1081 CCCCCCTGAC GAGCATCACA AAAATCGACG CTCAAGTCAG AGGTGGCGAA ACCCGACAGG 1141 ACTATAAAGA TACCAGGCGT TTCCCCCTGG AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC 1201 CCTGCCGCTT ACCGGATACC TGTCCGCCTT TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA 1261 TAGCTCACGC TGTAGGTATC TCAGTTCGGT GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT 1321 GCACGAACCC CCCGTTCAGC CCGACCGCTG CGCCTTATCC GGTAACTATC GTCTTGAGTC 1381 CAACCCGGTA AGACACGACT TATCGCCACT GGCAGCAGCC ACTGGTAACA GGATTAGCAG 1441 AGCGAGGTAT GTAGGCGGTG CTACAGAGTT CTTGAAGTGG TGGCCTAACT ACGGCTACAC 1501 TAGAAGAACA GTATTTGGTA TCTGCGCTCT GCTGAAGCCA GTTACCTTCG GAAAAAGAGT 1561 TGGTAGCTCT TGATCCGGCA AACAAACCAC CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA 1621 GCAGCAGATT ACGCGCAGAA AAAAAGGATC TCAAGAAGGC CTACTATTAG CAACAACGAT 1681 CCTTTGATCT TTTCTACGGG GTCTGACGCT CAGTGGAACG AAAACTCACG TTAAGGGATT 1741 TTGGTCATGA GATTATCAAA AAGGATCTTC ACCTAGATCC TTTTAAATTA AAAATGAAGT 1801 TTTAAATCAA TCTAAAGTAT ATATGAGTAA ACTTGGTCTG ACAGTTACCA ATGCTTAATC 1861 AGTGAGGCAC CTATCTCAGC GATCTGTCTA TTTCGTTCAT CCATAGTTGC CTGACTCCCC 1921 GTCGTGTAGA TAACTACGAT ACGGGAGGGC TTACCATCTG GCCCCAGTGC TGCAATGATA 1981 CCGCGAGAAC CACGCTCACC GGCTCCAGAT TTATCAGCAA TAAACCAGCC AGCCGGAAGG 2041 GCCGAGCGCA GAAGTGGTCC TGCAACTTTA TCCGCCTCCA TCCAGTCTAT TAATTGTTGC 2101 CGGGAAGCTA GAGTAAGTAG TTCGCCAGTT AATAGTTTGC GCAACGTTGT TGCCATTGCT 2161 ACAGGCATCG TGGTGTCACG CTCGTCGTTT GGTATGGCTT CATTCAGCTC CGGTTCCCAA 2221 CGATCAAGGC GAGTTACATG ATCCCCCATG TTGTGCAAAA AAGCGGTTAG CTCCTTCGGT 2281 CCTCCGATCG TTGTCAGAAG TAAGTTGGCC GCAGTGTTAT CACTCATGGT TATGGCAGCA 2341 CTGCATAATT CTCTTACTGT CATGCCATCC GTAAGATGCT TTTCTGTGAC TGGTGAGTAC 2401 TCAACCAAGT CATTCTGAGA ATAGTGTATG CGGCGACCGA GTTGCTCTTG CCCGGCGTCA 2461 ATACGGGATA ATACCGCGCC ACATAGCAGA ACTTTAAAAG TGCTCATCAT TGGAAAACGT 2521 TCTTCGGGGC GAAAACTCTC AAGGATCTTA CCGCTGTTGA GATCCAGTTC GATGTAACCC 2581 ACTCGTGCAC CCAACTGATC TTCAGCATCT TTTACTTTCA CCAGCGTTTC TGGGTGAGCA 2641 AAAACAGGAA GGCAAAATGC CGCAAAAAAG GGAATAAGGG CGACACGGAA ATGTTGAATA 2701 CTCATACTCT TCCTTTTTCA ATATTATTGA AGCATTTATC AGGGTTATTG TCTCATGAGC 2761 GGATACATAT TTGAATGTAT TTAGAAAAAT AAACAAATAG GGGTTCCGCG CACCTGCACC 2821 AGTCAGTAAA ACGACGGCCA GTAGTCAAAA GCCTCCGACC GGAGGCTTTT GACTTGGTCA 2881 GGTGGAGTGG GAG //