LOCUS       Exported     3202 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..3202
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      1240..1287
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     3135..3151
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      3152..3183
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             29..1168
                     /translation="MFLGVMIFGPIAALILKHTEKFWIHRIKSGFEMLVNNFYLGFLGFALIFPSFYLSIYLIGYIQLGLKLLVEIMQQYKLYPIAAIVIEPAKVLFLNNAINHGVLTPLGLQQVRDSGKSILFLLESNPGPGLGLLVAFLIFFFKRDKKLSSNAASSSPIHLFGGIHEVYFPFVLLKPVLILATIAVGVVGNGILQIFNAGTIAPVSPGSVIAGFLQINKTPLDVAGYALALVLSAVTSLLISLLLLSLTRKKQLKTLQEAQAQVAEMKQTPAKKPRQKDTPAIATKIDFSQVTFVCDAGMGSSTMGAAIFRKELKNQNIEDITVINKAIVDLKDEKVIITISQLYDRVKAKRADATIYTINQFLDKQGYLTIIEKIKNEKN"
                     /label="mtlA"
                     /note=""
     primer_bind     complement(1292..1308)
                     /note="common sequencing primer, one of multiple similar variants"
                     /label="M13 rev"
     rep_origin      complement(1375..1963)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(2154..3014)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(3015..3119)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
ORIGIN
        1 AAGGCGCTCG GATAATACTC GGTCTCAAAT GTTTTTAGGC GTGATGATCT TTGGCCCTAT
       61 CGCCGCCCTG ATTCTCAAAC ACACGGAAAA GTTTTGGATC CACCGCATCA AATCTGGCTT
      121 CGAGATGCTG GTCAACAATT TCTACCTCGG CTTTTTGGGT TTTGCCCTGA TTTTTCCGTC
      181 TTTTTACCTG AGCATCTATC TGATTGGCTA TATCCAGCTG GGCCTCAAAC TGCTGGTGGA
      241 GATTATGCAG CAGTACAAAC TGTACCCGAT TGCCGCGATC GTCATCGAAC CGGCAAAGGT
      301 CCTGTTCTTG AATAATGCAA TTAATCATGG CGTCTTGACC CCGCTGGGCC TGCAACAGGT
      361 TCGTGATTCT GGTAAAAGCA TTCTGTTCTT GCTGGAGTCA AACCCGGGTC CGGGTTTGGG
      421 TCTGCTGGTT GCTTTTCTGA TCTTCTTTTT CAAGCGTGAC AAGAAGCTCA GCAGCAACGC
      481 GGCATCAAGC TCCCCGATCC ATCTGTTTGG TGGTATTCAC GAGGTTTACT TTCCGTTTGT
      541 TCTTCTGAAA CCGGTTCTGA TCCTGGCGAC TATTGCGGTG GGCGTGGTTG GTAACGGTAT
      601 TTTGCAAATT TTCAATGCCG GTACCATTGC GCCTGTGAGC CCGGGCAGCG TGATTGCGGG
      661 CTTTCTTCAA ATTAACAAGA CCCCGCTGGA TGTGGCCGGT TATGCGTTGG CGTTGGTTCT
      721 GAGCGCTGTC ACCTCGCTGC TGATTTCCTT GCTGCTGCTG AGCCTGACTC GTAAGAAACA
      781 ACTGAAAACC CTGCAAGAAG CGCAGGCACA GGTGGCAGAG ATGAAACAAA CGCCGGCAAA
      841 AAAGCCGCGT CAAAAGGACA CCCCGGCAAT TGCGACCAAG ATCGATTTCA GCCAAGTGAC
      901 GTTTGTCTGT GATGCGGGTA TGGGCAGCAG CACGATGGGT GCGGCGATTT TCCGTAAGGA
      961 GCTGAAGAAT CAGAACATCG AGGACATCAC GGTCATTAAC AAAGCCATTG TGGATCTGAA
     1021 AGACGAAAAA GTTATCATTA CCATTAGCCA ACTGTATGAT CGTGTGAAAG CGAAACGCGC
     1081 GGATGCAACC ATTTACACGA TCAACCAATT CTTGGACAAA CAGGGCTACC TGACCATTAT
     1141 TGAAAAAATC AAAAACGAAA AGAACTGAAG AGCTTAGAGA CCGCTGGGCA CTATGCTTTA
     1201 CTCGCTGAGG TGTCAATCGT CGGAGCCGCT GAGCAATAAC TAGCATAACC CCTTGGGGCC
     1261 TCTAAACGGG TCTTGAGGGG TTTTTTGCAT GGTCATAGCT GTTTCCTGAG AGCTTGGCAG
     1321 GTGATGACAC ACATTAACAA ATTTCGTGAG GAGTCTCCAG AAGAATGCCA TTAATTTCCA
     1381 TAGGCTCCGC CCCCCTGACG AGCATCACAA AAATCGACGC TCAAGTCAGA GGTGGCGAAA
     1441 CCCGACAGGA CTATAAAGAT ACCAGGCGTT TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC
     1501 TGTTCCGACC CTGCCGCTTA CCGGATACCT GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC
     1561 GCTTTCTCAT AGCTCACGCT GTAGGTATCT CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT
     1621 GGGCTGTGTG CACGAACCCC CCGTTCAGCC CGACCGCTGC GCCTTATCCG GTAACTATCG
     1681 TCTTGAGTCC AACCCGGTAA GACACGACTT ATCGCCACTG GCAGCAGCCA CTGGTAACAG
     1741 GATTAGCAGA GCGAGGTATG TAGGCGGTGC TACAGAGTTC TTGAAGTGGT GGCCTAACTA
     1801 CGGCTACACT AGAAGAACAG TATTTGGTAT CTGCGCTCTG CTGAAGCCAG TTACCTTCGG
     1861 AAAAAGAGTT GGTAGCTCTT GATCCGGCAA ACAAACCACC GCTGGTAGCG GTGGTTTTTT
     1921 TGTTTGCAAG CAGCAGATTA CGCGCAGAAA AAAAGGATCT CAAGAAGGCC TACTATTAGC
     1981 AACAACGATC CTTTGATCTT TTCTACGGGG TCTGACGCTC AGTGGAACGA AAACTCACGT
     2041 TAAGGGATTT TGGTCATGAG ATTATCAAAA AGGATCTTCA CCTAGATCCT TTTAAATTAA
     2101 AAATGAAGTT TTAAATCAAT CTAAAGTATA TATGAGTAAA CTTGGTCTGA CAGTTACCAA
     2161 TGCTTAATCA GTGAGGCACC TATCTCAGCG ATCTGTCTAT TTCGTTCATC CATAGTTGCC
     2221 TGACTCCCCG TCGTGTAGAT AACTACGATA CGGGAGGGCT TACCATCTGG CCCCAGTGCT
     2281 GCAATGATAC CGCGAGAACC ACGCTCACCG GCTCCAGATT TATCAGCAAT AAACCAGCCA
     2341 GCCGGAAGGG CCGAGCGCAG AAGTGGTCCT GCAACTTTAT CCGCCTCCAT CCAGTCTATT
     2401 AATTGTTGCC GGGAAGCTAG AGTAAGTAGT TCGCCAGTTA ATAGTTTGCG CAACGTTGTT
     2461 GCCATTGCTA CAGGCATCGT GGTGTCACGC TCGTCGTTTG GTATGGCTTC ATTCAGCTCC
     2521 GGTTCCCAAC GATCAAGGCG AGTTACATGA TCCCCCATGT TGTGCAAAAA AGCGGTTAGC
     2581 TCCTTCGGTC CTCCGATCGT TGTCAGAAGT AAGTTGGCCG CAGTGTTATC ACTCATGGTT
     2641 ATGGCAGCAC TGCATAATTC TCTTACTGTC ATGCCATCCG TAAGATGCTT TTCTGTGACT
     2701 GGTGAGTACT CAACCAAGTC ATTCTGAGAA TAGTGTATGC GGCGACCGAG TTGCTCTTGC
     2761 CCGGCGTCAA TACGGGATAA TACCGCGCCA CATAGCAGAA CTTTAAAAGT GCTCATCATT
     2821 GGAAAACGTT CTTCGGGGCG AAAACTCTCA AGGATCTTAC CGCTGTTGAG ATCCAGTTCG
     2881 ATGTAACCCA CTCGTGCACC CAACTGATCT TCAGCATCTT TTACTTTCAC CAGCGTTTCT
     2941 GGGTGAGCAA AAACAGGAAG GCAAAATGCC GCAAAAAAGG GAATAAGGGC GACACGGAAA
     3001 TGTTGAATAC TCATACTCTT CCTTTTTCAA TATTATTGAA GCATTTATCA GGGTTATTGT
     3061 CTCATGAGCG GATACATATT TGAATGTATT TAGAAAAATA AACAAATAGG GGTTCCGCGC
     3121 ACCTGCACCA GTCAGTAAAA CGACGGCCAG TAGTCAAAAG CCTCCGACCG GAGGCTTTTG
     3181 ACTTGGTCAG GTGGAGTGGG AG
//