LOCUS       Exported     3268 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..3268
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      1306..1353
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     3201..3217
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      3218..3249
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             29..1234
                     /label="MPN657"
                     /note=""
                     /translation="MDWLKRWFTRKDQDKTETTSASKRAKITSSLLMFSALYEAKKPLKYTIVYILALVNAFFLLVFIQQTGLYSFGISSLTQGFARLLFVLLKNLEDGQRNLVFNIFYWLFYVIVNIPLIIFSYKKIGKRFTILSTHYVVASNVFGFIFSIIPGANQLPSMLSAVHHTEFWEDAKKAEGVDQSALFVPFLWNDTSQGNVIISTFIYAGIYGFVNGTSLAILYILGSCAGGADFLTQYFARKKNRSVGPILFYVNTFILIIAILMGSFVAGSIVLQDIPDYKKSAWQVNLFFSPNLIATFFSVLFTGTVVSHLFPRYNFAEIKVFTDKIEEVRLALLNDKATHSLSIQETMGGYSLAKKRMIVSVTMYVEIPNLIRIIRKIDKDCLVSITRIRGIDGYIYLRSQD"
     primer_bind     complement(1358..1374)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(1441..2029)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(2220..3080)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(3081..3185)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
ORIGIN
        1 AAGAATTACT GACCCCTCGG GGTCTCAAAT GGACTGGCTG AAACGTTGGT TTACCCGTAA
       61 GGACCAAGAT AAAACCGAAA CCACCAGCGC TAGCAAACGT GCGAAGATTA CCAGCTCGTT
      121 ACTGATGTTC AGCGCCTTGT ATGAAGCGAA AAAGCCGTTG AAGTATACCA TTGTCTATAT
      181 TTTGGCGCTG GTCAATGCGT TTTTTCTGCT GGTGTTCATC CAGCAAACGG GTCTGTATAG
      241 CTTCGGCATC AGCAGCTTGA CGCAGGGCTT TGCGCGTCTG CTGTTTGTCC TGCTTAAGAA
      301 CCTGGAGGAC GGCCAGCGTA ACTTGGTTTT CAATATCTTT TATTGGTTGT TTTACGTCAT
      361 TGTTAATATT CCGCTGATCA TCTTCAGCTA TAAGAAAATT GGTAAAAGAT TTACGATCCT
      421 GAGCACTCAC TATGTGGTCG CAAGCAACGT TTTTGGCTTC ATTTTTAGCA TTATTCCGGG
      481 TGCAAACCAA CTGCCTAGCA TGCTCTCCGC GGTCCACCAC ACGGAGTTTT GGGAGGACGC
      541 AAAGAAAGCG GAAGGCGTGG ATCAAAGCGC GCTGTTCGTT CCGTTCCTGT GGAACGATAC
      601 GAGCCAGGGT AACGTTATTA TTAGCACCTT CATCTATGCG GGTATCTACG GCTTCGTTAA
      661 TGGCACCAGC TTGGCGATCC TGTACATCCT GGGTAGCTGC GCTGGCGGTG CTGATTTCCT
      721 GACCCAATAT TTCGCCCGTA AAAAGAACCG TAGCGTTGGT CCGATCCTGT TTTATGTTAA
      781 CACTTTTATT CTGATTATCG CTATCCTGAT GGGTAGCTTC GTGGCGGGCA GCATCGTCCT
      841 GCAAGATATT CCGGACTACA AAAAGAGCGC GTGGCAAGTT AACCTGTTCT TTTCTCCGAA
      901 CCTGATCGCC ACTTTCTTTA GCGTTCTGTT CACCGGCACC GTGGTCAGCC ATCTGTTCCC
      961 GCGTTACAAC TTCGCAGAGA TTAAAGTCTT TACGGACAAA ATCGAGGAAG TCAGACTGGC
     1021 ACTCCTGAAT GATAAGGCGA CCCATTCCCT GTCCATTCAA GAAACCATGG GCGGTTATAG
     1081 CCTGGCTAAG AAACGCATGA TCGTTAGCGT GACGATGTAT GTAGAGATTC CGAATCTGAT
     1141 TCGTATCATC CGCAAGATCG ACAAGGACTG CCTGGTTTCC ATCACCCGCA TTCGTGGCAT
     1201 TGACGGCTAT ATTTATTTAC GTAGCCAAGA TTGAAGAGCT TAGAGACCCC GGTTGTACCT
     1261 ATCGAGTGCG CTGAGGTGTC AATCGTCGGA GCCGCTGAGC AATAACTAGC ATAACCCCTT
     1321 GGGGCCTCTA AACGGGTCTT GAGGGGTTTT TTGCATGGTC ATAGCTGTTT CCTGAGAGCT
     1381 TGGCAGGTGA TGACACACAT TAACAAATTT CGTGAGGAGT CTCCAGAAGA ATGCCATTAA
     1441 TTTCCATAGG CTCCGCCCCC CTGACGAGCA TCACAAAAAT CGACGCTCAA GTCAGAGGTG
     1501 GCGAAACCCG ACAGGACTAT AAAGATACCA GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG
     1561 CTCTCCTGTT CCGACCCTGC CGCTTACCGG ATACCTGTCC GCCTTTCTCC CTTCGGGAAG
     1621 CGTGGCGCTT TCTCATAGCT CACGCTGTAG GTATCTCAGT TCGGTGTAGG TCGTTCGCTC
     1681 CAAGCTGGGC TGTGTGCACG AACCCCCCGT TCAGCCCGAC CGCTGCGCCT TATCCGGTAA
     1741 CTATCGTCTT GAGTCCAACC CGGTAAGACA CGACTTATCG CCACTGGCAG CAGCCACTGG
     1801 TAACAGGATT AGCAGAGCGA GGTATGTAGG CGGTGCTACA GAGTTCTTGA AGTGGTGGCC
     1861 TAACTACGGC TACACTAGAA GAACAGTATT TGGTATCTGC GCTCTGCTGA AGCCAGTTAC
     1921 CTTCGGAAAA AGAGTTGGTA GCTCTTGATC CGGCAAACAA ACCACCGCTG GTAGCGGTGG
     1981 TTTTTTTGTT TGCAAGCAGC AGATTACGCG CAGAAAAAAA GGATCTCAAG AAGGCCTACT
     2041 ATTAGCAACA ACGATCCTTT GATCTTTTCT ACGGGGTCTG ACGCTCAGTG GAACGAAAAC
     2101 TCACGTTAAG GGATTTTGGT CATGAGATTA TCAAAAAGGA TCTTCACCTA GATCCTTTTA
     2161 AATTAAAAAT GAAGTTTTAA ATCAATCTAA AGTATATATG AGTAAACTTG GTCTGACAGT
     2221 TACCAATGCT TAATCAGTGA GGCACCTATC TCAGCGATCT GTCTATTTCG TTCATCCATA
     2281 GTTGCCTGAC TCCCCGTCGT GTAGATAACT ACGATACGGG AGGGCTTACC ATCTGGCCCC
     2341 AGTGCTGCAA TGATACCGCG AGAACCACGC TCACCGGCTC CAGATTTATC AGCAATAAAC
     2401 CAGCCAGCCG GAAGGGCCGA GCGCAGAAGT GGTCCTGCAA CTTTATCCGC CTCCATCCAG
     2461 TCTATTAATT GTTGCCGGGA AGCTAGAGTA AGTAGTTCGC CAGTTAATAG TTTGCGCAAC
     2521 GTTGTTGCCA TTGCTACAGG CATCGTGGTG TCACGCTCGT CGTTTGGTAT GGCTTCATTC
     2581 AGCTCCGGTT CCCAACGATC AAGGCGAGTT ACATGATCCC CCATGTTGTG CAAAAAAGCG
     2641 GTTAGCTCCT TCGGTCCTCC GATCGTTGTC AGAAGTAAGT TGGCCGCAGT GTTATCACTC
     2701 ATGGTTATGG CAGCACTGCA TAATTCTCTT ACTGTCATGC CATCCGTAAG ATGCTTTTCT
     2761 GTGACTGGTG AGTACTCAAC CAAGTCATTC TGAGAATAGT GTATGCGGCG ACCGAGTTGC
     2821 TCTTGCCCGG CGTCAATACG GGATAATACC GCGCCACATA GCAGAACTTT AAAAGTGCTC
     2881 ATCATTGGAA AACGTTCTTC GGGGCGAAAA CTCTCAAGGA TCTTACCGCT GTTGAGATCC
     2941 AGTTCGATGT AACCCACTCG TGCACCCAAC TGATCTTCAG CATCTTTTAC TTTCACCAGC
     3001 GTTTCTGGGT GAGCAAAAAC AGGAAGGCAA AATGCCGCAA AAAAGGGAAT AAGGGCGACA
     3061 CGGAAATGTT GAATACTCAT ACTCTTCCTT TTTCAATATT ATTGAAGCAT TTATCAGGGT
     3121 TATTGTCTCA TGAGCGGATA CATATTTGAA TGTATTTAGA AAAATAAACA AATAGGGGTT
     3181 CCGCGCACCT GCACCAGTCA GTAAAACGAC GGCCAGTAGT CAAAAGCCTC CGACCGGAGG
     3241 CTTTTGACTT GGTCAGGTGG AGTGGGAG
//