LOCUS Exported 2776 bp DNA circular SYN 24-JUL-2020 DEFINITION synthetic linear DNA ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2001) AUTHORS Keoni Gandall TITLE Direct Submission JOURNAL Exported from Poly. FEATURES Location/Qualifiers terminator 1..2776 /label="T7 terminator" /note="transcription terminator for bacteriophage T7 RNA polymerase" terminator 814..861 /label="T7 terminator" /note="transcription terminator for bacteriophage T7 RNA polymerase" primer_bind 2709..2725 /label="M13 for" /note="common sequencing primer, one of multiple similar variants" terminator 2726..2757 /label="tonB terminator" /note="bidirectional E. coli tonB-P14 transcription terminator" CDS 29..742 /translation="MANAPKGVKFSTSQTTEEIVRAKVSELVEQYDLIIGIPIDKEISASYANWKLVEKDYASKFHVLDARAVEMIIDWLINDIKGWLNTNPYSREGLDRFVEQYRKKTAAVLFVTDTKPLVAGGRLSNLKSFIIKSLKFHLLISFLGENGKLQFFDKARSAHDAHKLAVKFLKKQLLKHSAKLKRGAFLTTVFDEQTNTNLVQEFDKLLDHSINIEQSLLSPVICTHTGLNSYVIVLQGE" /label="degV" /note="" primer_bind complement(866..882) /label="M13 rev" /note="common sequencing primer, one of multiple similar variants" rep_origin complement(949..1537) /label="ori" /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(1728..2588) /label="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*" promoter complement(2589..2693) /label="bla promoter" /note="ampicillin resistance gene promoter" ORIGIN 1 AATGCGTCAT TTTACACGGC GGTCTCAAAT GGCAAATGCG CCGAAAGGTG TGAAATTCAG 61 CACGAGCCAG ACCACGGAGG AGATCGTCCG TGCAAAGGTA AGCGAGCTGG TGGAGCAGTA 121 CGATCTGATT ATTGGCATTC CGATCGACAA AGAGATTAGC GCTAGCTACG CGAACTGGAA 181 GCTGGTGGAA AAAGATTACG CGAGCAAGTT CCACGTTCTG GATGCCCGCG CGGTGGAGAT 241 GATTATTGAC TGGCTGATCA ATGATATCAA AGGCTGGCTG AACACCAATC CGTATTCCAG 301 AGAGGGCCTG GATCGTTTCG TGGAGCAGTA TCGTAAAAAG ACCGCGGCGG TGCTGTTCGT 361 GACCGACACG AAGCCGCTGG TGGCGGGTGG TCGTTTGAGC AATCTTAAGA GCTTCATCAT 421 CAAAAGCCTG AAGTTCCATT TACTGATCTC TTTCCTGGGT GAAAACGGTA AACTGCAATT 481 TTTTGATAAA GCACGCAGCG CCCATGACGC GCACAAACTG GCGGTCAAGT TCCTGAAAAA 541 ACAATTGTTG AAGCACAGCG CCAAGCTGAA GCGTGGTGCA TTCCTGACCA CCGTTTTTGA 601 CGAGCAAACC AATACGAACT TGGTGCAGGA GTTCGACAAG CTGCTGGACC ATAGCATTAA 661 TATTGAACAG AGCTTGCTCA GCCCGGTCAT TTGCACGCAC ACCGGTCTGA ACAGCTACGT 721 TATTGTGCTG CAAGGTGAGT GAAGAGCTTA GAGACCGCCA TTGGAAAACT GAACGTCGCT 781 GAGGTGTCAA TCGTCGGAGC CGCTGAGCAA TAACTAGCAT AACCCCTTGG GGCCTCTAAA 841 CGGGTCTTGA GGGGTTTTTT GCATGGTCAT AGCTGTTTCC TGAGAGCTTG GCAGGTGATG 901 ACACACATTA ACAAATTTCG TGAGGAGTCT CCAGAAGAAT GCCATTAATT TCCATAGGCT 961 CCGCCCCCCT GACGAGCATC ACAAAAATCG ACGCTCAAGT CAGAGGTGGC GAAACCCGAC 1021 AGGACTATAA AGATACCAGG CGTTTCCCCC TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC 1081 GACCCTGCCG CTTACCGGAT ACCTGTCCGC CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC 1141 TCATAGCTCA CGCTGTAGGT ATCTCAGTTC GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG 1201 TGTGCACGAA CCCCCCGTTC AGCCCGACCG CTGCGCCTTA TCCGGTAACT ATCGTCTTGA 1261 GTCCAACCCG GTAAGACACG ACTTATCGCC ACTGGCAGCA GCCACTGGTA ACAGGATTAG 1321 CAGAGCGAGG TATGTAGGCG GTGCTACAGA GTTCTTGAAG TGGTGGCCTA ACTACGGCTA 1381 CACTAGAAGA ACAGTATTTG GTATCTGCGC TCTGCTGAAG CCAGTTACCT TCGGAAAAAG 1441 AGTTGGTAGC TCTTGATCCG GCAAACAAAC CACCGCTGGT AGCGGTGGTT TTTTTGTTTG 1501 CAAGCAGCAG ATTACGCGCA GAAAAAAAGG ATCTCAAGAA GGCCTACTAT TAGCAACAAC 1561 GATCCTTTGA TCTTTTCTAC GGGGTCTGAC GCTCAGTGGA ACGAAAACTC ACGTTAAGGG 1621 ATTTTGGTCA TGAGATTATC AAAAAGGATC TTCACCTAGA TCCTTTTAAA TTAAAAATGA 1681 AGTTTTAAAT CAATCTAAAG TATATATGAG TAAACTTGGT CTGACAGTTA CCAATGCTTA 1741 ATCAGTGAGG CACCTATCTC AGCGATCTGT CTATTTCGTT CATCCATAGT TGCCTGACTC 1801 CCCGTCGTGT AGATAACTAC GATACGGGAG GGCTTACCAT CTGGCCCCAG TGCTGCAATG 1861 ATACCGCGAG AACCACGCTC ACCGGCTCCA GATTTATCAG CAATAAACCA GCCAGCCGGA 1921 AGGGCCGAGC GCAGAAGTGG TCCTGCAACT TTATCCGCCT CCATCCAGTC TATTAATTGT 1981 TGCCGGGAAG CTAGAGTAAG TAGTTCGCCA GTTAATAGTT TGCGCAACGT TGTTGCCATT 2041 GCTACAGGCA TCGTGGTGTC ACGCTCGTCG TTTGGTATGG CTTCATTCAG CTCCGGTTCC 2101 CAACGATCAA GGCGAGTTAC ATGATCCCCC ATGTTGTGCA AAAAAGCGGT TAGCTCCTTC 2161 GGTCCTCCGA TCGTTGTCAG AAGTAAGTTG GCCGCAGTGT TATCACTCAT GGTTATGGCA 2221 GCACTGCATA ATTCTCTTAC TGTCATGCCA TCCGTAAGAT GCTTTTCTGT GACTGGTGAG 2281 TACTCAACCA AGTCATTCTG AGAATAGTGT ATGCGGCGAC CGAGTTGCTC TTGCCCGGCG 2341 TCAATACGGG ATAATACCGC GCCACATAGC AGAACTTTAA AAGTGCTCAT CATTGGAAAA 2401 CGTTCTTCGG GGCGAAAACT CTCAAGGATC TTACCGCTGT TGAGATCCAG TTCGATGTAA 2461 CCCACTCGTG CACCCAACTG ATCTTCAGCA TCTTTTACTT TCACCAGCGT TTCTGGGTGA 2521 GCAAAAACAG GAAGGCAAAA TGCCGCAAAA AAGGGAATAA GGGCGACACG GAAATGTTGA 2581 ATACTCATAC TCTTCCTTTT TCAATATTAT TGAAGCATTT ATCAGGGTTA TTGTCTCATG 2641 AGCGGATACA TATTTGAATG TATTTAGAAA AATAAACAAA TAGGGGTTCC GCGCACCTGC 2701 ACCAGTCAGT AAAACGACGG CCAGTAGTCA AAAGCCTCCG ACCGGAGGCT TTTGACTTGG 2761 TCAGGTGGAG TGGGAG //