LOCUS Exported 2917 bp DNA circular SYN 24-JUL-2020 DEFINITION synthetic linear DNA ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2001) AUTHORS Keoni Gandall TITLE Direct Submission JOURNAL Exported from Poly. FEATURES Location/Qualifiers terminator 1..2917 /label="T7 terminator" /note="transcription terminator for bacteriophage T7 RNA polymerase" terminator 955..1002 /label="T7 terminator" /note="transcription terminator for bacteriophage T7 RNA polymerase" primer_bind 2850..2866 /label="M13 for" /note="common sequencing primer, one of multiple similar variants" terminator 2867..2898 /label="tonB terminator" /note="bidirectional E. coli tonB-P14 transcription terminator" CDS 29..883 /label="cysA" /note="" /translation="MSLNAKNKRSLDYCLQWPDFCQSKKASKLIVKLNKKHPKRRHYKDPEAKHYDVLFKGVCKAVTNGITNQLICDHIDLKIAPGEFVVILGKSGSGKTSLLSLISALDRPTSGVSFVCGRSTICCNDAQLTSLRNKNVGYIFQQYGLLRDLNVDDNIKLAVPFKKRHNNNLEELLERLELKEHRNKKITKLSGGQQQRVAIARALIKEPRILFGDEPTGAVNVDISKKILQFFVEYNRDKGTTIVLVTHNEKIVELAKRVIKIHDGKIVADYLNQRPKTINEINWV" primer_bind complement(1007..1023) /label="M13 rev" /note="common sequencing primer, one of multiple similar variants" rep_origin complement(1090..1678) /label="ori" /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(1869..2729) /label="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*" promoter complement(2730..2834) /label="bla promoter" /note="ampicillin resistance gene promoter" ORIGIN 1 AAGGCGCTCG GATAATACTC GGTCTCAAAT GAGCCTGAAC GCCAAGAACA AACGTTCCTT 61 GGACTACTGT TTGCAGTGGC CGGACTTTTG TCAAAGCAAG AAAGCTAGCA AGCTGATTGT 121 CAAGCTGAAT AAAAAGCACC CAAAGCGTCG TCACTACAAG GACCCGGAGG CCAAACATTA 181 CGATGTTCTG TTCAAAGGCG TCTGCAAGGC CGTGACCAAC GGCATTACCA ACCAGCTGAT 241 TTGCGATCAT ATTGATCTGA AAATTGCCCC GGGTGAATTC GTAGTCATCC TGGGCAAAAG 301 CGGCTCCGGC AAGACGTCCC TGCTGAGCCT GATCTCAGCA TTGGACCGCC CGACCAGCGG 361 TGTGAGCTTC GTCTGCGGCC GTTCCACCAT TTGTTGCAAC GACGCGCAGC TGACCTCCCT 421 GCGCAATAAA AATGTGGGTT ACATTTTCCA ACAGTATGGT CTGCTGCGTG ACTTGAATGT 481 GGACGATAAC ATCAAACTGG CTGTTCCGTT CAAGAAGCGT CACAATAATA ACCTGGAAGA 541 ACTGCTGGAA CGTTTGGAAC TGAAGGAACA TAGAAATAAG AAGATCACCA AACTGAGCGG 601 CGGTCAGCAG CAGCGCGTGG CGATCGCACG TGCGTTGATT AAAGAGCCGC GCATCTTGTT 661 CGGCGACGAA CCAACCGGCG CCGTGAACGT TGATATTAGC AAGAAAATCC TGCAGTTTTT 721 CGTGGAGTAC AATCGTGATA AAGGCACTAC CATCGTCCTG GTGACCCACA ATGAAAAAAT 781 CGTTGAACTG GCTAAAAGAG TGATCAAGAT TCACGACGGT AAAATTGTCG CAGACTATCT 841 GAATCAACGT CCAAAGACTA TTAACGAAAT CAACTGGGTG TGAAGAGCTT AGAGACCGCA 901 GCGATTCGGA TACTACTCGC TGAGGTGTCA ATCGTCGGAG CCGCTGAGCA ATAACTAGCA 961 TAACCCCTTG GGGCCTCTAA ACGGGTCTTG AGGGGTTTTT TGCATGGTCA TAGCTGTTTC 1021 CTGAGAGCTT GGCAGGTGAT GACACACATT AACAAATTTC GTGAGGAGTC TCCAGAAGAA 1081 TGCCATTAAT TTCCATAGGC TCCGCCCCCC TGACGAGCAT CACAAAAATC GACGCTCAAG 1141 TCAGAGGTGG CGAAACCCGA CAGGACTATA AAGATACCAG GCGTTTCCCC CTGGAAGCTC 1201 CCTCGTGCGC TCTCCTGTTC CGACCCTGCC GCTTACCGGA TACCTGTCCG CCTTTCTCCC 1261 TTCGGGAAGC GTGGCGCTTT CTCATAGCTC ACGCTGTAGG TATCTCAGTT CGGTGTAGGT 1321 CGTTCGCTCC AAGCTGGGCT GTGTGCACGA ACCCCCCGTT CAGCCCGACC GCTGCGCCTT 1381 ATCCGGTAAC TATCGTCTTG AGTCCAACCC GGTAAGACAC GACTTATCGC CACTGGCAGC 1441 AGCCACTGGT AACAGGATTA GCAGAGCGAG GTATGTAGGC GGTGCTACAG AGTTCTTGAA 1501 GTGGTGGCCT AACTACGGCT ACACTAGAAG AACAGTATTT GGTATCTGCG CTCTGCTGAA 1561 GCCAGTTACC TTCGGAAAAA GAGTTGGTAG CTCTTGATCC GGCAAACAAA CCACCGCTGG 1621 TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA GATTACGCGC AGAAAAAAAG GATCTCAAGA 1681 AGGCCTACTA TTAGCAACAA CGATCCTTTG ATCTTTTCTA CGGGGTCTGA CGCTCAGTGG 1741 AACGAAAACT CACGTTAAGG GATTTTGGTC ATGAGATTAT CAAAAAGGAT CTTCACCTAG 1801 ATCCTTTTAA ATTAAAAATG AAGTTTTAAA TCAATCTAAA GTATATATGA GTAAACTTGG 1861 TCTGACAGTT ACCAATGCTT AATCAGTGAG GCACCTATCT CAGCGATCTG TCTATTTCGT 1921 TCATCCATAG TTGCCTGACT CCCCGTCGTG TAGATAACTA CGATACGGGA GGGCTTACCA 1981 TCTGGCCCCA GTGCTGCAAT GATACCGCGA GAACCACGCT CACCGGCTCC AGATTTATCA 2041 GCAATAAACC AGCCAGCCGG AAGGGCCGAG CGCAGAAGTG GTCCTGCAAC TTTATCCGCC 2101 TCCATCCAGT CTATTAATTG TTGCCGGGAA GCTAGAGTAA GTAGTTCGCC AGTTAATAGT 2161 TTGCGCAACG TTGTTGCCAT TGCTACAGGC ATCGTGGTGT CACGCTCGTC GTTTGGTATG 2221 GCTTCATTCA GCTCCGGTTC CCAACGATCA AGGCGAGTTA CATGATCCCC CATGTTGTGC 2281 AAAAAAGCGG TTAGCTCCTT CGGTCCTCCG ATCGTTGTCA GAAGTAAGTT GGCCGCAGTG 2341 TTATCACTCA TGGTTATGGC AGCACTGCAT AATTCTCTTA CTGTCATGCC ATCCGTAAGA 2401 TGCTTTTCTG TGACTGGTGA GTACTCAACC AAGTCATTCT GAGAATAGTG TATGCGGCGA 2461 CCGAGTTGCT CTTGCCCGGC GTCAATACGG GATAATACCG CGCCACATAG CAGAACTTTA 2521 AAAGTGCTCA TCATTGGAAA ACGTTCTTCG GGGCGAAAAC TCTCAAGGAT CTTACCGCTG 2581 TTGAGATCCA GTTCGATGTA ACCCACTCGT GCACCCAACT GATCTTCAGC ATCTTTTACT 2641 TTCACCAGCG TTTCTGGGTG AGCAAAAACA GGAAGGCAAA ATGCCGCAAA AAAGGGAATA 2701 AGGGCGACAC GGAAATGTTG AATACTCATA CTCTTCCTTT TTCAATATTA TTGAAGCATT 2761 TATCAGGGTT ATTGTCTCAT GAGCGGATAC ATATTTGAAT GTATTTAGAA AAATAAACAA 2821 ATAGGGGTTC CGCGCACCTG CACCAGTCAG TAAAACGACG GCCAGTAGTC AAAAGCCTCC 2881 GACCGGAGGC TTTTGACTTG GTCAGGTGGA GTGGGAG //