LOCUS       Exported     4940 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..4940
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      38..85
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     1933..1949
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      1950..1981
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             100..4939
                     /label="ileS"
                     /note="Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile)."
                     /translation="MSDYKSTLNLPETGFPMRGDLAKREPGMLARWTDDDLYGIIRAAKKGKKTFILHDGPPYANGSIHIGHSVNKILKDIIVKSKGLSGYDSPYVPGWDCHGLPIELKVEQEYGKPGEKFTAAEFRAKCREYAATQVDGQRKDFIRLGVLGDWSHPYLTMDFKTEANIIRALGKIIGNGHLHKGAKPVHWCVDCRSALAEAEVEYYDKTSPSIDVAFQAVDQDALKAKFAVSNVNGPISLVIWTTTPWTLPANRAISIAPDFDYALVQIDGQAVILAKDLVESVMQRIGVTDYTILGTVKGAELELLRFTHPFMGFDVPAILGDHVTLDAGTGAVHTAPGHGPDDYVIGQKYGLETANPVGPDGTYLPGTYPTLDGVNVFKANDIVVALLQEKGALLHVEKMQHSYPCCWRHKTPIIFRATPQWFVSMDQKGLRAQSLKEIKGVQWIPDWGQARIESMVANRPDWCISRQRTWGVPMSLFVHKDTEELHPRTLELMEEVAKRVEVDGIQAWWDLDAKEILGDEADQYVKVPDTLDVWFDSGSTHSSVVDVRPEFAGHAADMYLEGSDQHRGWFMSSLMISTAMKGKAPYRQVLTHGFTVDGQGRKMSKSIGNTVSPQDVMNKLGADILRLWVASTDYTGEMAVSDEILKRAADSYRRIRNTARFLLANLNGFDPAKDMVKPEEMVVLDRWAVGCAKAAQEDILKAYEAYDFHEVVQRLMRFCSVEMGSFYLDIIKDRQYTAKADSVARRSCQTALYHIAEALVRWMAPILSFTADEVWGYLPGEREKYVFTGEWYEGLFGLADSEAMNDAFWDELLKVRGEVNKVIEQARADKKVGGSLEAAVTLYAEPELSAKLTALGDELRFVLLTSGATVADYNDAPADAQQSEVLKGLKVALSKAEGEKCPRCWHYTQDVGKVAEHAEICGRCVSNVAGDGEKRKFA"
     primer_bind     complement(90..106)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(173..761)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(952..1812)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(1813..1917)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
     misc_feature    4881..4940
                     /label="FreeGenes Barcode"
                     /note="FreeGenes Barcode. Defined as I-SceI site flanked by 21 base pair random sequences."
ORIGIN
        1 CGCTGAGGTG TCAATCGTCG GAGCCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC
       61 TAAACGGGTC TTGAGGGGTT TTTTGCATGG TCATAGCTGT TTCCTGAGAG CTTGGCAGGT
      121 GATGACACAC ATTAACAAAT TTCGTGAGGA GTCTCCAGAA GAATGCCATT AATTTCCATA
      181 GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC
      241 CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG
      301 TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC
      361 TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG
      421 GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC
      481 TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA
      541 TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG
      601 GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA
      661 AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG
      721 TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGGCCTA CTATTAGCAA
      781 CAACGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA
      841 AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA
      901 ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAAACT TGGTCTGACA GTTACCAATG
      961 CTTAATCAGT GAGGCACCTA TCTCAGCGAT CTGTCTATTT CGTTCATCCA TAGTTGCCTG
     1021 ACTCCCCGTC GTGTAGATAA CTACGATACG GGAGGGCTTA CCATCTGGCC CCAGTGCTGC
     1081 AATGATACCG CGAGAACCAC GCTCACCGGC TCCAGATTTA TCAGCAATAA ACCAGCCAGC
     1141 CGGAAGGGCC GAGCGCAGAA GTGGTCCTGC AACTTTATCC GCCTCCATCC AGTCTATTAA
     1201 TTGTTGCCGG GAAGCTAGAG TAAGTAGTTC GCCAGTTAAT AGTTTGCGCA ACGTTGTTGC
     1261 CATTGCTACA GGCATCGTGG TGTCACGCTC GTCGTTTGGT ATGGCTTCAT TCAGCTCCGG
     1321 TTCCCAACGA TCAAGGCGAG TTACATGATC CCCCATGTTG TGCAAAAAAG CGGTTAGCTC
     1381 CTTCGGTCCT CCGATCGTTG TCAGAAGTAA GTTGGCCGCA GTGTTATCAC TCATGGTTAT
     1441 GGCAGCACTG CATAATTCTC TTACTGTCAT GCCATCCGTA AGATGCTTTT CTGTGACTGG
     1501 TGAGTACTCA ACCAAGTCAT TCTGAGAATA GTGTATGCGG CGACCGAGTT GCTCTTGCCC
     1561 GGCGTCAATA CGGGATAATA CCGCGCCACA TAGCAGAACT TTAAAAGTGC TCATCATTGG
     1621 AAAACGTTCT TCGGGGCGAA AACTCTCAAG GATCTTACCG CTGTTGAGAT CCAGTTCGAT
     1681 GTAACCCACT CGTGCACCCA ACTGATCTTC AGCATCTTTT ACTTTCACCA GCGTTTCTGG
     1741 GTGAGCAAAA ACAGGAAGGC AAAATGCCGC AAAAAAGGGA ATAAGGGCGA CACGGAAATG
     1801 TTGAATACTC ATACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT
     1861 CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC
     1921 CTGCACCAGT CAGTAAAACG ACGGCCAGTA GTCAAAAGCC TCCGACCGGA GGCTTTTGAC
     1981 TTGGTTCAGG TGGAGTGGGA GAAAGGGGCC GTCAATATCA GGGTCTCTAA TGTCAGACTA
     2041 CAAATCAACC CTAAACCTAC CGGAAACCGG ATTCCCGATG CGTGGAGACC TAGCAAAACG
     2101 TGAACCGGGA ATGCTAGCAC GTTGGACCGA CGACGACCTA TACGGAATCA TCCGTGCAGC
     2161 AAAAAAAGGA AAAAAAACCT TCATCCTACA CGACGGACCG CCGTACGCAA ACGGATCAAT
     2221 CCACATCGGA CACTCAGTCA ACAAAATCCT AAAAGACATC ATCGTCAAAT CAAAAGGACT
     2281 ATCAGGATAC GACTCACCGT ACGTCCCGGG ATGGGACTGC CACGGACTAC CGATCGAACT
     2341 AAAAGTCGAA CAGGAATACG GAAAACCGGG AGAAAAATTC ACCGCAGCAG AATTCCGTGC
     2401 AAAATGCCGT GAATACGCAG CAACCCAGGT CGACGGACAG CGTAAAGACT TCATCCGTCT
     2461 AGGAGTCCTA GGAGACTGGT CACACCCGTA CCTAACCATG GACTTCAAAA CCGAAGCAAA
     2521 CATCATCCGT GCACTAGGAA AAATCATCGG AAACGGACAC CTACACAAAG GAGCAAAACC
     2581 GGTCCACTGG TGCGTCGACT GCCGTTCAGC ACTAGCAGAA GCAGAAGTCG AATACTACGA
     2641 CAAAACCTCA CCGTCAATCG ACGTCGCATT CCAGGCAGTC GACCAGGACG CACTAAAAGC
     2701 AAAATTCGCA GTCTCAAACG TCAACGGACC GATCTCACTA GTCATCTGGA CCACCACCCC
     2761 GTGGACCCTA CCGGCAAACC GTGCAATCTC AATCGCACCG GACTTCGACT ACGCACTAGT
     2821 CCAGATCGAC GGACAGGCAG TCATCCTAGC AAAAGACCTA GTCGAATCAG TCATGCAGCG
     2881 TATCGGAGTC ACCGACTACA CCATCCTAGG AACCGTCAAA GGAGCAGAAC TAGAACTACT
     2941 ACGTTTCACC CACCCGTTCA TGGGATTCGA CGTCCCGGCA ATCCTAGGAG ACCACGTCAC
     3001 CCTAGACGCA GGAACCGGAG CAGTCCACAC CGCACCGGGA CACGGACCGG ACGACTACGT
     3061 CATCGGACAG AAATACGGAC TAGAAACCGC AAACCCGGTC GGACCGGACG GAACCTACCT
     3121 ACCGGGAACC TACCCGACCC TAGACGGAGT CAACGTCTTC AAAGCAAACG ACATCGTCGT
     3181 CGCACTACTA CAGGAAAAAG GAGCACTACT ACACGTCGAA AAAATGCAGC ACTCATACCC
     3241 GTGCTGCTGG CGTCACAAAA CCCCGATCAT CTTCCGTGCA ACCCCGCAGT GGTTCGTCTC
     3301 AATGGACCAG AAAGGACTAC GTGCACAGTC ACTAAAAGAA ATCAAAGGAG TCCAGTGGAT
     3361 CCCGGACTGG GGACAGGCAC GTATCGAATC AATGGTCGCA AACCGTCCGG ACTGGTGCAT
     3421 CTCACGTCAG CGTACCTGGG GAGTCCCGAT GTCACTATTC GTCCACAAAG ACACCGAAGA
     3481 ACTACACCCG CGTACCCTAG AACTAATGGA AGAAGTCGCA AAACGTGTCG AAGTCGACGG
     3541 AATCCAGGCA TGGTGGGACC TAGACGCAAA AGAAATCCTA GGAGACGAAG CAGACCAGTA
     3601 CGTCAAAGTC CCGGACACCC TAGACGTCTG GTTCGACTCA GGATCAACCC ACTCATCAGT
     3661 CGTCGACGTC CGTCCGGAAT TCGCAGGACA CGCAGCAGAC ATGTACCTAG AAGGATCAGA
     3721 CCAGCACCGT GGATGGTTCA TGTCATCACT AATGATCTCA ACCGCAATGA AAGGAAAAGC
     3781 ACCGTACCGT CAGGTCCTAA CCCACGGATT CACCGTCGAC GGACAGGGAC GTAAAATGTC
     3841 AAAATCAATC GGAAACACCG TCTCACCGCA GGACGTCATG AACAAACTAG GAGCAGACAT
     3901 CCTACGTCTA TGGGTCGCAT CAACCGACTA CACCGGAGAA ATGGCAGTCT CAGACGAAAT
     3961 CCTAAAACGT GCAGCAGACT CATACCGTCG TATCCGTAAC ACCGCACGTT TCCTACTAGC
     4021 AAACCTAAAC GGATTCGACC CGGCAAAAGA CATGGTCAAA CCGGAAGAAA TGGTCGTCCT
     4081 AGACCGTTGG GCAGTCGGAT GCGCAAAAGC AGCACAGGAA GACATCCTAA AAGCATACGA
     4141 AGCATACGAC TTCCACGAAG TCGTCCAGCG TCTAATGCGT TTCTGCTCAG TCGAAATGGG
     4201 ATCATTCTAC CTAGACATCA TCAAAGACCG TCAGTACACC GCAAAAGCAG ACTCAGTCGC
     4261 ACGTCGTTCA TGCCAGACCG CACTATACCA CATCGCAGAA GCACTAGTCC GTTGGATGGC
     4321 ACCGATCCTA TCATTCACCG CAGACGAAGT CTGGGGATAC CTACCGGGAG AACGTGAAAA
     4381 ATACGTCTTC ACCGGAGAAT GGTACGAAGG ACTATTCGGA CTAGCAGACT CAGAAGCAAT
     4441 GAACGACGCA TTCTGGGACG AACTACTAAA AGTCCGTGGA GAAGTCAACA AAGTCATCGA
     4501 ACAGGCACGT GCAGACAAAA AAGTCGGAGG ATCACTAGAA GCAGCAGTCA CCCTATACGC
     4561 AGAACCGGAA CTATCAGCAA AACTAACCGC ACTAGGAGAC GAACTACGTT TCGTCCTACT
     4621 AACCTCAGGA GCAACCGTCG CAGACTACAA CGACGCACCG GCAGACGCAC AGCAGTCAGA
     4681 AGTCCTAAAA GGACTAAAAG TCGCACTATC AAAAGCAGAA GGAGAAAAAT GCCCGCGTTG
     4741 CTGGCACTAC ACCCAGGACG TCGGAAAAGT CGCAGAACAC GCAGAAATCT GCGGACGTTG
     4801 CGTCTCAAAC GTCGCAGGAG ACGGAGAAAA ACGTAAATTC GCATGAAGAG CTTCGAGACC
     4861 AAGAATTACT GACCCCTCGG TATCCGCATT CGCTGTTCAG CTAGGGATAA CAGGGTAATA
     4921 TCTTCCTGCA CGCAGTAAGT
//