LOCUS       Exported     4478 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..4478
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      38..85
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     1933..1949
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      1950..1981
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
                     /label="tonB terminator"
     CDS             2030..4384
                     /label="lptD"
                     /note="Together with LptE, is involved in the assembly of lipopolysaccharide (LPS) at the surface of the outer membrane. Contributes to n-hexane resistance."
                     /translation="MKKRIPTLLATMIATALYSQQGLAADLASQCMLGVPSYDRPLVQGDTNDLPVTINADHAKGDYPDDAVFTGSVDIMQGNSRLQADEVQLHQKEAPGQPEPVRTVDALGNVHYDDNQVILKGPKGWANLNTKDTNVWEGDYQMVGRQGRGKADLMKQRGENRYTILDNGSFTSCLPGSDTWSVVGSEIIHDREEQVAEIWNARFKVGPVPIFYSPYLQLPVGDKRRSGFLIPNAKYTTTNYFEFYLPYYWNIAPNMDATITPHYMHRRGNIMWENEFRYLSQAGAGLMELDYLPSDKVYEDEHPNDDSSRRWLFYWNHSGVMDQVWRFNVDYTKVSDPSYFNDFDNKYGSSTDGYATQKFSVGYAVQNFNATVSTKQFQVFSEQNTSSYSAEPQLDVNYYQNDVGPFDTRIYGQAVHFVNTRDDMPEATRVHLEPTINLPLSNNWGSINTEAKLLATHYQQTNLDWYNSRNTTKLDESVNRVMPQFKVDGKMVFERDMEMLAPGYTQTLEPRAQYLYVPYRDQSDIYNYDSSLLQSDYSGLFRDRTYGGLDRIASANQVTTGVTSRIYDDAAVERFNISVGQIYYFTESRTGDDNITWENDDKTGSLVWAGDTYWRISERWGLRGGIQYDTRLDNVATSNSSIEYRRDEDRLVQLNYRYASPEYIQATLPKYYSTAEQYKNGISQVGAVASWPIADRWSIVGAYYYDTNANKQADSMLGVQYSSCCYAIRVGYERKLNGWDNDKQHAVYDNAIGFNIELRGLSSNYGLGTQEMLRSNILPYQNTL"
     primer_bind     complement(90..106)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(173..761)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(952..1812)
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
     promoter        complement(1813..1917)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
     misc_feature    4419..4478
                     /label="FreeGenes Barcode"
                     /note="FreeGenes Barcode. Defined as I-SceI site flanked by 21 base pair random sequences."
ORIGIN
        1 CGCTGAGGTG TCAATCGTCG GAGCCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC
       61 TAAACGGGTC TTGAGGGGTT TTTTGCATGG TCATAGCTGT TTCCTGAGAG CTTGGCAGGT
      121 GATGACACAC ATTAACAAAT TTCGTGAGGA GTCTCCAGAA GAATGCCATT AATTTCCATA
      181 GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC
      241 CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG
      301 TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC
      361 TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG
      421 GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC
      481 TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA
      541 TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG
      601 GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA
      661 AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG
      721 TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGGCCTA CTATTAGCAA
      781 CAACGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA
      841 AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA
      901 ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAAACT TGGTCTGACA GTTACCAATG
      961 CTTAATCAGT GAGGCACCTA TCTCAGCGAT CTGTCTATTT CGTTCATCCA TAGTTGCCTG
     1021 ACTCCCCGTC GTGTAGATAA CTACGATACG GGAGGGCTTA CCATCTGGCC CCAGTGCTGC
     1081 AATGATACCG CGAGAACCAC GCTCACCGGC TCCAGATTTA TCAGCAATAA ACCAGCCAGC
     1141 CGGAAGGGCC GAGCGCAGAA GTGGTCCTGC AACTTTATCC GCCTCCATCC AGTCTATTAA
     1201 TTGTTGCCGG GAAGCTAGAG TAAGTAGTTC GCCAGTTAAT AGTTTGCGCA ACGTTGTTGC
     1261 CATTGCTACA GGCATCGTGG TGTCACGCTC GTCGTTTGGT ATGGCTTCAT TCAGCTCCGG
     1321 TTCCCAACGA TCAAGGCGAG TTACATGATC CCCCATGTTG TGCAAAAAAG CGGTTAGCTC
     1381 CTTCGGTCCT CCGATCGTTG TCAGAAGTAA GTTGGCCGCA GTGTTATCAC TCATGGTTAT
     1441 GGCAGCACTG CATAATTCTC TTACTGTCAT GCCATCCGTA AGATGCTTTT CTGTGACTGG
     1501 TGAGTACTCA ACCAAGTCAT TCTGAGAATA GTGTATGCGG CGACCGAGTT GCTCTTGCCC
     1561 GGCGTCAATA CGGGATAATA CCGCGCCACA TAGCAGAACT TTAAAAGTGC TCATCATTGG
     1621 AAAACGTTCT TCGGGGCGAA AACTCTCAAG GATCTTACCG CTGTTGAGAT CCAGTTCGAT
     1681 GTAACCCACT CGTGCACCCA ACTGATCTTC AGCATCTTTT ACTTTCACCA GCGTTTCTGG
     1741 GTGAGCAAAA ACAGGAAGGC AAAATGCCGC AAAAAAGGGA ATAAGGGCGA CACGGAAATG
     1801 TTGAATACTC ATACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT
     1861 CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC
     1921 CTGCACCAGT CAGTAAAACG ACGGCCAGTA GTCAAAAGCC TCCGACCGGA GGCTTTTGAC
     1981 TTGGTTCAGG TGGAGTGGGA GAAAGCACTC TTAGGCCTCT GGGTCTCTAA TGAAAAAACG
     2041 TATCCCGACC CTACTAGCAA CCATGATCGC AACCGCACTA TACTCACAGC AGGGACTAGC
     2101 AGCAGACCTA GCATCACAGT GCATGCTAGG AGTCCCGTCA TACGACCGTC CGCTAGTCCA
     2161 GGGAGACACC AACGACCTAC CGGTCACCAT CAACGCAGAC CACGCAAAAG GAGACTACCC
     2221 GGACGACGCA GTCTTCACCG GATCAGTCGA CATCATGCAG GGAAACTCAC GTCTACAGGC
     2281 AGACGAAGTC CAGCTACACC AGAAAGAAGC ACCGGGACAG CCGGAACCGG TCCGTACCGT
     2341 CGACGCACTA GGAAACGTCC ACTACGACGA CAACCAGGTC ATCCTAAAAG GACCGAAAGG
     2401 ATGGGCAAAC CTAAACACCA AAGACACCAA CGTCTGGGAA GGAGACTACC AGATGGTCGG
     2461 ACGTCAGGGA CGTGGAAAAG CAGACCTAAT GAAACAGCGT GGAGAAAACC GTTACACCAT
     2521 CCTAGACAAC GGATCATTCA CCTCATGCCT ACCGGGATCA GACACCTGGT CAGTCGTCGG
     2581 ATCAGAAATC ATCCACGACC GTGAAGAACA GGTCGCAGAA ATCTGGAACG CACGTTTCAA
     2641 AGTCGGACCG GTCCCGATCT TCTACTCACC GTACCTACAG CTACCGGTCG GAGACAAACG
     2701 TCGTTCAGGA TTCCTAATCC CGAACGCAAA ATACACCACC ACCAACTACT TCGAATTCTA
     2761 CCTACCGTAC TACTGGAACA TCGCACCGAA CATGGACGCA ACCATCACCC CGCACTACAT
     2821 GCACCGTCGT GGAAACATCA TGTGGGAAAA CGAATTCCGT TACCTATCAC AGGCAGGAGC
     2881 AGGACTAATG GAACTAGACT ACCTACCGTC AGACAAAGTC TACGAAGACG AACACCCGAA
     2941 CGACGACTCA TCACGTCGTT GGCTATTCTA CTGGAACCAC TCAGGAGTCA TGGACCAGGT
     3001 CTGGCGTTTC AACGTCGACT ACACCAAAGT CTCAGACCCG TCATACTTCA ACGACTTCGA
     3061 CAACAAATAC GGATCATCAA CCGACGGATA CGCAACCCAG AAATTCTCAG TCGGATACGC
     3121 AGTCCAGAAC TTCAACGCAA CCGTCTCAAC CAAACAGTTC CAGGTCTTCT CAGAACAGAA
     3181 CACCTCATCA TACTCAGCAG AACCGCAGCT AGACGTCAAC TACTACCAGA ACGACGTCGG
     3241 ACCGTTCGAC ACCCGTATCT ACGGACAGGC AGTCCACTTC GTCAACACCC GTGACGACAT
     3301 GCCGGAAGCA ACCCGTGTCC ACCTAGAACC GACCATCAAC CTACCGCTAT CAAACAACTG
     3361 GGGATCAATC AACACCGAAG CAAAACTACT AGCAACCCAC TACCAGCAGA CCAACCTAGA
     3421 CTGGTACAAC TCACGTAACA CCACCAAACT AGACGAATCA GTCAACCGTG TCATGCCGCA
     3481 GTTCAAAGTC GACGGAAAAA TGGTCTTCGA ACGTGACATG GAAATGCTAG CACCGGGATA
     3541 CACCCAGACC CTAGAACCGC GTGCACAGTA CCTATACGTC CCGTACCGTG ACCAGTCAGA
     3601 CATCTACAAC TACGACTCAT CACTACTACA GTCAGACTAC TCAGGACTAT TCCGTGACCG
     3661 TACCTACGGA GGACTAGACC GTATCGCATC AGCAAACCAG GTCACCACCG GAGTCACCTC
     3721 ACGTATCTAC GACGACGCAG CAGTCGAACG TTTCAACATC TCAGTCGGAC AGATCTACTA
     3781 CTTCACCGAA TCACGTACCG GAGACGACAA CATCACCTGG GAAAACGACG ACAAAACCGG
     3841 ATCACTAGTC TGGGCAGGAG ACACCTACTG GCGTATCTCA GAACGTTGGG GACTACGTGG
     3901 AGGAATCCAG TACGACACCC GTCTAGACAA CGTCGCAACC TCAAACTCAT CAATCGAATA
     3961 CCGTCGTGAC GAAGACCGTC TAGTCCAGCT AAACTACCGT TACGCATCAC CGGAATACAT
     4021 CCAGGCAACC CTACCGAAAT ACTACTCAAC CGCAGAACAG TACAAAAACG GAATCTCACA
     4081 GGTCGGAGCA GTCGCATCAT GGCCGATCGC AGACCGTTGG TCAATCGTCG GAGCATACTA
     4141 CTACGACACC AACGCAAACA AACAGGCAGA CTCAATGCTA GGAGTCCAGT ACTCATCATG
     4201 CTGCTACGCA ATCCGTGTCG GATACGAACG TAAACTAAAC GGATGGGACA ACGACAAACA
     4261 GCACGCAGTC TACGACAACG CAATCGGATT CAACATCGAA CTACGTGGAC TATCATCAAA
     4321 CTACGGACTA GGAACCCAGG AAATGCTACG TTCAAACATC CTACCGTACC AGAACACCCT
     4381 ATGAAGAGCT TCGAGACCAT TGACGGGAAC TACACTCGCC GTGAAAACCG GTGAGGTCAT
     4441 AGGGATAACA GGGTAATTTT GGGCCCTGTC CTTTATGA
//