LOCUS Exported 3890 bp DNA circular SYN 24-JUL-2020 DEFINITION synthetic linear DNA ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2001) AUTHORS Keoni Gandall TITLE Direct Submission JOURNAL Exported from Poly. FEATURES Location/Qualifiers terminator 1..3890 /label="T7 terminator" /note="transcription terminator for bacteriophage T7 RNA polymerase" terminator 1927..1974 /label="T7 terminator" /note="transcription terminator for bacteriophage T7 RNA polymerase" primer_bind 3822..3838 /label="M13 for" /note="common sequencing primer, one of multiple similar variants" terminator 3839..3870 /note="bidirectional E. coli tonB-P14 transcription terminator" /label="tonB terminator" CDS 100..3889 /label="ftsI" /note="Essential cell division protein that catalyzes cross-linking of the peptidoglycan cell wall at the division septum (PubMed:1103132, PubMed:6450748, PubMed:9614966, PubMed:3531167, PubMed:7030331). Required for localization of FtsN (PubMed:9282742)." /translation="MKAAAKTQKPKRQEEHANFISWRFALLCGCILLALAFLLGRVAWLQVISPDMLVKEGDMRSLRVQQVSTSRGMITDRSGRPLAVSVPVKAIWADPKEVHDAGGISVGDRWKALANALNIPLDQLSARINANPKGRFIYLARQVNPDMADYIKKLKLPGIHLREESRRYYPSGEVTAHLIGFTNVDSQGIEGVEKSFDKWLTGQPGERIVRKDRYGRVIEDISSTDSQAAHNLALSIDERLQALVYRELNNAVAFNKAESGSAVLVDVNTGEVLAMANSPSYNPNNLSGTPKEAMRNRTITDVFEPGSTVKPMVVMTALQRGVVRENSVLNTIPYRINGHEIKDVARYSELTLTGVLQKSSNVGVSKLALAMPSSALVDTYSRFGLGKATNLGLVGERSGLYPQKQRWSDIERATFSFGYGLMVTPLQLARVYATIGSYGIYRPLSITKVDPPVPGERVFPESIVRTVVHMMESVALPGGGGVKAAIKGYRIAIKTGTAKKVGPDGRYINKYIAYTAGVAPASQPRFALVVVINDPQAGKYYGGAVSAPVFGAIMGGVLRTMNIEPDALTTGDKNEFVINQGEGTGGRS" primer_bind complement(1979..1995) /label="M13 rev" /note="common sequencing primer, one of multiple similar variants" rep_origin complement(2062..2650) /label="ori" /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(2841..3701) /label="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*" promoter complement(3702..3806) /label="bla promoter" /note="ampicillin resistance gene promoter" misc_feature 1830..1889 /label="FreeGenes Barcode" /note="FreeGenes Barcode. Defined as I-SceI site flanked by 21 base pair random sequences." ORIGIN 1 AAAGCACTCT TAGGCCTCTG GGTCTCTAAT GAAAGCAGCA GCAAAAACCC AGAAACCGAA 61 ACGTCAGGAA GAACACGCAA ACTTCATCTC ATGGCGTTTC GCACTACTAT GCGGATGCAT 121 CCTACTAGCA CTAGCATTCC TACTAGGACG TGTCGCATGG CTACAGGTCA TCTCACCGGA 181 CATGCTAGTC AAAGAAGGAG ACATGCGTTC ACTACGTGTC CAGCAGGTCT CAACCTCACG 241 TGGAATGATC ACCGACCGTT CAGGACGTCC GCTAGCAGTC TCAGTCCCGG TCAAAGCAAT 301 CTGGGCAGAC CCGAAAGAAG TCCACGACGC AGGAGGAATC TCAGTCGGAG ACCGTTGGAA 361 AGCACTAGCA AACGCACTAA ACATCCCGCT AGACCAGCTA TCAGCACGTA TCAACGCAAA 421 CCCGAAAGGA CGTTTCATCT ACCTAGCACG TCAGGTCAAC CCGGACATGG CAGACTACAT 481 CAAAAAACTA AAACTACCGG GAATCCACCT ACGTGAAGAA TCACGTCGTT ACTACCCGTC 541 AGGAGAAGTC ACCGCACACC TAATCGGATT CACCAACGTC GACTCACAGG GAATCGAAGG 601 AGTCGAAAAA TCATTCGACA AATGGCTAAC CGGACAGCCG GGAGAACGTA TCGTCCGTAA 661 AGACCGTTAC GGACGTGTCA TCGAAGACAT CTCATCAACC GACTCACAGG CAGCACACAA 721 CCTAGCACTA TCAATCGACG AACGTCTACA GGCACTAGTC TACCGTGAAC TAAACAACGC 781 AGTCGCATTC AACAAAGCAG AATCAGGATC AGCAGTCCTA GTCGACGTCA ACACCGGAGA 841 AGTCCTAGCA ATGGCAAACT CACCGTCATA CAACCCGAAC AACCTATCAG GAACCCCGAA 901 AGAAGCAATG CGTAACCGTA CCATCACCGA CGTCTTCGAA CCGGGATCAA CCGTCAAACC 961 GATGGTCGTC ATGACCGCAC TACAGCGTGG AGTCGTCCGT GAAAACTCAG TCCTAAACAC 1021 CATCCCGTAC CGTATCAACG GACACGAAAT CAAAGACGTC GCACGTTACT CAGAACTAAC 1081 CCTAACCGGA GTCCTACAGA AATCATCAAA CGTCGGAGTC TCAAAACTAG CACTAGCAAT 1141 GCCGTCATCA GCACTAGTCG ACACCTACTC ACGTTTCGGA CTAGGAAAAG CAACCAACCT 1201 AGGACTAGTC GGAGAACGTT CAGGACTATA CCCGCAGAAA CAGCGTTGGT CAGACATCGA 1261 ACGTGCAACC TTCTCATTCG GATACGGACT AATGGTCACC CCGCTACAGC TAGCACGTGT 1321 CTACGCAACC ATCGGATCAT ACGGAATCTA CCGTCCGCTA TCAATCACCA AAGTCGACCC 1381 GCCGGTCCCG GGAGAACGTG TCTTCCCGGA ATCAATCGTC CGTACCGTCG TCCACATGAT 1441 GGAATCAGTC GCACTACCGG GAGGAGGAGG AGTCAAAGCA GCAATCAAAG GATACCGTAT 1501 CGCAATCAAA ACCGGAACCG CAAAAAAAGT CGGACCGGAC GGACGTTACA TCAACAAATA 1561 CATCGCATAC ACCGCAGGAG TCGCACCGGC ATCACAGCCG CGTTTCGCAC TAGTCGTCGT 1621 CATCAACGAC CCGCAGGCAG GAAAATACTA CGGAGGAGCA GTCTCAGCAC CGGTCTTCGG 1681 AGCAATCATG GGAGGAGTCC TACGTACCAT GAACATCGAA CCGGACGCAC TAACCACCGG 1741 AGACAAAAAC GAATTCGTCA TCAACCAGGG AGAAGGAACC GGAGGACGTT CATGAAGAGC 1801 TTCGAGACCA CGGAGCCCTT ATTGTAACCT AGTCAGGGGA TGGTGTACGA TAGGGATAAC 1861 AGGGTAATGA ATAGACATCT AGGCTCTGTC GCTGAGGTGT CAATCGTCGG AGCCGCTGAG 1921 CAATAACTAG CATAACCCCT TGGGGCCTCT AAACGGGTCT TGAGGGGTTT TTTGCATGGT 1981 CATAGCTGTT TCCTGAGAGC TTGGCAGGTG ATGACACACA TTAACAAATT TCGTGAGGAG 2041 TCTCCAGAAG AATGCCATTA ATTTCCATAG GCTCCGCCCC CCTGACGAGC ATCACAAAAA 2101 TCGACGCTCA AGTCAGAGGT GGCGAAACCC GACAGGACTA TAAAGATACC AGGCGTTTCC 2161 CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT TCCGACCCTG CCGCTTACCG GATACCTGTC 2221 CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT TTCTCATAGC TCACGCTGTA GGTATCTCAG 2281 TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG CTGTGTGCAC GAACCCCCCG TTCAGCCCGA 2341 CCGCTGCGCC TTATCCGGTA ACTATCGTCT TGAGTCCAAC CCGGTAAGAC ACGACTTATC 2401 GCCACTGGCA GCAGCCACTG GTAACAGGAT TAGCAGAGCG AGGTATGTAG GCGGTGCTAC 2461 AGAGTTCTTG AAGTGGTGGC CTAACTACGG CTACACTAGA AGAACAGTAT TTGGTATCTG 2521 CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA AAGAGTTGGT AGCTCTTGAT CCGGCAAACA 2581 AACCACCGCT GGTAGCGGTG GTTTTTTTGT TTGCAAGCAG CAGATTACGC GCAGAAAAAA 2641 AGGATCTCAA GAAGGCCTAC TATTAGCAAC AACGATCCTT TGATCTTTTC TACGGGGTCT 2701 GACGCTCAGT GGAACGAAAA CTCACGTTAA GGGATTTTGG TCATGAGATT ATCAAAAAGG 2761 ATCTTCACCT AGATCCTTTT AAATTAAAAA TGAAGTTTTA AATCAATCTA AAGTATATAT 2821 GAGTAAACTT GGTCTGACAG TTACCAATGC TTAATCAGTG AGGCACCTAT CTCAGCGATC 2881 TGTCTATTTC GTTCATCCAT AGTTGCCTGA CTCCCCGTCG TGTAGATAAC TACGATACGG 2941 GAGGGCTTAC CATCTGGCCC CAGTGCTGCA ATGATACCGC GAGAACCACG CTCACCGGCT 3001 CCAGATTTAT CAGCAATAAA CCAGCCAGCC GGAAGGGCCG AGCGCAGAAG TGGTCCTGCA 3061 ACTTTATCCG CCTCCATCCA GTCTATTAAT TGTTGCCGGG AAGCTAGAGT AAGTAGTTCG 3121 CCAGTTAATA GTTTGCGCAA CGTTGTTGCC ATTGCTACAG GCATCGTGGT GTCACGCTCG 3181 TCGTTTGGTA TGGCTTCATT CAGCTCCGGT TCCCAACGAT CAAGGCGAGT TACATGATCC 3241 CCCATGTTGT GCAAAAAAGC GGTTAGCTCC TTCGGTCCTC CGATCGTTGT CAGAAGTAAG 3301 TTGGCCGCAG TGTTATCACT CATGGTTATG GCAGCACTGC ATAATTCTCT TACTGTCATG 3361 CCATCCGTAA GATGCTTTTC TGTGACTGGT GAGTACTCAA CCAAGTCATT CTGAGAATAG 3421 TGTATGCGGC GACCGAGTTG CTCTTGCCCG GCGTCAATAC GGGATAATAC CGCGCCACAT 3481 AGCAGAACTT TAAAAGTGCT CATCATTGGA AAACGTTCTT CGGGGCGAAA ACTCTCAAGG 3541 ATCTTACCGC TGTTGAGATC CAGTTCGATG TAACCCACTC GTGCACCCAA CTGATCTTCA 3601 GCATCTTTTA CTTTCACCAG CGTTTCTGGG TGAGCAAAAA CAGGAAGGCA AAATGCCGCA 3661 AAAAAGGGAA TAAGGGCGAC ACGGAAATGT TGAATACTCA TACTCTTCCT TTTTCAATAT 3721 TATTGAAGCA TTTATCAGGG TTATTGTCTC ATGAGCGGAT ACATATTTGA ATGTATTTAG 3781 AAAAATAAAC AAATAGGGGT TCCGCGCACC TGCACCAGTC AGTAAAACGA CGGCCAGTAG 3841 TCAAAAGCCT CCGACCGGAG GCTTTTGACT TGGTTCAGGT GGAGTGGGAG //