LOCUS       Exported     3890 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..3890
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      1927..1974
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     3822..3838
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      3839..3870
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
                     /label="tonB terminator"
     CDS             100..3889
                     /label="ftsI"
                     /note="Essential cell division protein that catalyzes cross-linking of the peptidoglycan cell wall at the division septum (PubMed:1103132, PubMed:6450748, PubMed:9614966, PubMed:3531167, PubMed:7030331). Required for localization of FtsN (PubMed:9282742)."
                     /translation="MKAAAKTQKPKRQEEHANFISWRFALLCGCILLALAFLLGRVAWLQVISPDMLVKEGDMRSLRVQQVSTSRGMITDRSGRPLAVSVPVKAIWADPKEVHDAGGISVGDRWKALANALNIPLDQLSARINANPKGRFIYLARQVNPDMADYIKKLKLPGIHLREESRRYYPSGEVTAHLIGFTNVDSQGIEGVEKSFDKWLTGQPGERIVRKDRYGRVIEDISSTDSQAAHNLALSIDERLQALVYRELNNAVAFNKAESGSAVLVDVNTGEVLAMANSPSYNPNNLSGTPKEAMRNRTITDVFEPGSTVKPMVVMTALQRGVVRENSVLNTIPYRINGHEIKDVARYSELTLTGVLQKSSNVGVSKLALAMPSSALVDTYSRFGLGKATNLGLVGERSGLYPQKQRWSDIERATFSFGYGLMVTPLQLARVYATIGSYGIYRPLSITKVDPPVPGERVFPESIVRTVVHMMESVALPGGGGVKAAIKGYRIAIKTGTAKKVGPDGRYINKYIAYTAGVAPASQPRFALVVVINDPQAGKYYGGAVSAPVFGAIMGGVLRTMNIEPDALTTGDKNEFVINQGEGTGGRS"
     primer_bind     complement(1979..1995)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(2062..2650)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(2841..3701)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(3702..3806)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
     misc_feature    1830..1889
                     /label="FreeGenes Barcode"
                     /note="FreeGenes Barcode. Defined as I-SceI site flanked by 21 base pair random sequences."
ORIGIN
        1 AAAGCACTCT TAGGCCTCTG GGTCTCTAAT GAAAGCAGCA GCAAAAACCC AGAAACCGAA
       61 ACGTCAGGAA GAACACGCAA ACTTCATCTC ATGGCGTTTC GCACTACTAT GCGGATGCAT
      121 CCTACTAGCA CTAGCATTCC TACTAGGACG TGTCGCATGG CTACAGGTCA TCTCACCGGA
      181 CATGCTAGTC AAAGAAGGAG ACATGCGTTC ACTACGTGTC CAGCAGGTCT CAACCTCACG
      241 TGGAATGATC ACCGACCGTT CAGGACGTCC GCTAGCAGTC TCAGTCCCGG TCAAAGCAAT
      301 CTGGGCAGAC CCGAAAGAAG TCCACGACGC AGGAGGAATC TCAGTCGGAG ACCGTTGGAA
      361 AGCACTAGCA AACGCACTAA ACATCCCGCT AGACCAGCTA TCAGCACGTA TCAACGCAAA
      421 CCCGAAAGGA CGTTTCATCT ACCTAGCACG TCAGGTCAAC CCGGACATGG CAGACTACAT
      481 CAAAAAACTA AAACTACCGG GAATCCACCT ACGTGAAGAA TCACGTCGTT ACTACCCGTC
      541 AGGAGAAGTC ACCGCACACC TAATCGGATT CACCAACGTC GACTCACAGG GAATCGAAGG
      601 AGTCGAAAAA TCATTCGACA AATGGCTAAC CGGACAGCCG GGAGAACGTA TCGTCCGTAA
      661 AGACCGTTAC GGACGTGTCA TCGAAGACAT CTCATCAACC GACTCACAGG CAGCACACAA
      721 CCTAGCACTA TCAATCGACG AACGTCTACA GGCACTAGTC TACCGTGAAC TAAACAACGC
      781 AGTCGCATTC AACAAAGCAG AATCAGGATC AGCAGTCCTA GTCGACGTCA ACACCGGAGA
      841 AGTCCTAGCA ATGGCAAACT CACCGTCATA CAACCCGAAC AACCTATCAG GAACCCCGAA
      901 AGAAGCAATG CGTAACCGTA CCATCACCGA CGTCTTCGAA CCGGGATCAA CCGTCAAACC
      961 GATGGTCGTC ATGACCGCAC TACAGCGTGG AGTCGTCCGT GAAAACTCAG TCCTAAACAC
     1021 CATCCCGTAC CGTATCAACG GACACGAAAT CAAAGACGTC GCACGTTACT CAGAACTAAC
     1081 CCTAACCGGA GTCCTACAGA AATCATCAAA CGTCGGAGTC TCAAAACTAG CACTAGCAAT
     1141 GCCGTCATCA GCACTAGTCG ACACCTACTC ACGTTTCGGA CTAGGAAAAG CAACCAACCT
     1201 AGGACTAGTC GGAGAACGTT CAGGACTATA CCCGCAGAAA CAGCGTTGGT CAGACATCGA
     1261 ACGTGCAACC TTCTCATTCG GATACGGACT AATGGTCACC CCGCTACAGC TAGCACGTGT
     1321 CTACGCAACC ATCGGATCAT ACGGAATCTA CCGTCCGCTA TCAATCACCA AAGTCGACCC
     1381 GCCGGTCCCG GGAGAACGTG TCTTCCCGGA ATCAATCGTC CGTACCGTCG TCCACATGAT
     1441 GGAATCAGTC GCACTACCGG GAGGAGGAGG AGTCAAAGCA GCAATCAAAG GATACCGTAT
     1501 CGCAATCAAA ACCGGAACCG CAAAAAAAGT CGGACCGGAC GGACGTTACA TCAACAAATA
     1561 CATCGCATAC ACCGCAGGAG TCGCACCGGC ATCACAGCCG CGTTTCGCAC TAGTCGTCGT
     1621 CATCAACGAC CCGCAGGCAG GAAAATACTA CGGAGGAGCA GTCTCAGCAC CGGTCTTCGG
     1681 AGCAATCATG GGAGGAGTCC TACGTACCAT GAACATCGAA CCGGACGCAC TAACCACCGG
     1741 AGACAAAAAC GAATTCGTCA TCAACCAGGG AGAAGGAACC GGAGGACGTT CATGAAGAGC
     1801 TTCGAGACCA CGGAGCCCTT ATTGTAACCT AGTCAGGGGA TGGTGTACGA TAGGGATAAC
     1861 AGGGTAATGA ATAGACATCT AGGCTCTGTC GCTGAGGTGT CAATCGTCGG AGCCGCTGAG
     1921 CAATAACTAG CATAACCCCT TGGGGCCTCT AAACGGGTCT TGAGGGGTTT TTTGCATGGT
     1981 CATAGCTGTT TCCTGAGAGC TTGGCAGGTG ATGACACACA TTAACAAATT TCGTGAGGAG
     2041 TCTCCAGAAG AATGCCATTA ATTTCCATAG GCTCCGCCCC CCTGACGAGC ATCACAAAAA
     2101 TCGACGCTCA AGTCAGAGGT GGCGAAACCC GACAGGACTA TAAAGATACC AGGCGTTTCC
     2161 CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT TCCGACCCTG CCGCTTACCG GATACCTGTC
     2221 CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT TTCTCATAGC TCACGCTGTA GGTATCTCAG
     2281 TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG CTGTGTGCAC GAACCCCCCG TTCAGCCCGA
     2341 CCGCTGCGCC TTATCCGGTA ACTATCGTCT TGAGTCCAAC CCGGTAAGAC ACGACTTATC
     2401 GCCACTGGCA GCAGCCACTG GTAACAGGAT TAGCAGAGCG AGGTATGTAG GCGGTGCTAC
     2461 AGAGTTCTTG AAGTGGTGGC CTAACTACGG CTACACTAGA AGAACAGTAT TTGGTATCTG
     2521 CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA AAGAGTTGGT AGCTCTTGAT CCGGCAAACA
     2581 AACCACCGCT GGTAGCGGTG GTTTTTTTGT TTGCAAGCAG CAGATTACGC GCAGAAAAAA
     2641 AGGATCTCAA GAAGGCCTAC TATTAGCAAC AACGATCCTT TGATCTTTTC TACGGGGTCT
     2701 GACGCTCAGT GGAACGAAAA CTCACGTTAA GGGATTTTGG TCATGAGATT ATCAAAAAGG
     2761 ATCTTCACCT AGATCCTTTT AAATTAAAAA TGAAGTTTTA AATCAATCTA AAGTATATAT
     2821 GAGTAAACTT GGTCTGACAG TTACCAATGC TTAATCAGTG AGGCACCTAT CTCAGCGATC
     2881 TGTCTATTTC GTTCATCCAT AGTTGCCTGA CTCCCCGTCG TGTAGATAAC TACGATACGG
     2941 GAGGGCTTAC CATCTGGCCC CAGTGCTGCA ATGATACCGC GAGAACCACG CTCACCGGCT
     3001 CCAGATTTAT CAGCAATAAA CCAGCCAGCC GGAAGGGCCG AGCGCAGAAG TGGTCCTGCA
     3061 ACTTTATCCG CCTCCATCCA GTCTATTAAT TGTTGCCGGG AAGCTAGAGT AAGTAGTTCG
     3121 CCAGTTAATA GTTTGCGCAA CGTTGTTGCC ATTGCTACAG GCATCGTGGT GTCACGCTCG
     3181 TCGTTTGGTA TGGCTTCATT CAGCTCCGGT TCCCAACGAT CAAGGCGAGT TACATGATCC
     3241 CCCATGTTGT GCAAAAAAGC GGTTAGCTCC TTCGGTCCTC CGATCGTTGT CAGAAGTAAG
     3301 TTGGCCGCAG TGTTATCACT CATGGTTATG GCAGCACTGC ATAATTCTCT TACTGTCATG
     3361 CCATCCGTAA GATGCTTTTC TGTGACTGGT GAGTACTCAA CCAAGTCATT CTGAGAATAG
     3421 TGTATGCGGC GACCGAGTTG CTCTTGCCCG GCGTCAATAC GGGATAATAC CGCGCCACAT
     3481 AGCAGAACTT TAAAAGTGCT CATCATTGGA AAACGTTCTT CGGGGCGAAA ACTCTCAAGG
     3541 ATCTTACCGC TGTTGAGATC CAGTTCGATG TAACCCACTC GTGCACCCAA CTGATCTTCA
     3601 GCATCTTTTA CTTTCACCAG CGTTTCTGGG TGAGCAAAAA CAGGAAGGCA AAATGCCGCA
     3661 AAAAAGGGAA TAAGGGCGAC ACGGAAATGT TGAATACTCA TACTCTTCCT TTTTCAATAT
     3721 TATTGAAGCA TTTATCAGGG TTATTGTCTC ATGAGCGGAT ACATATTTGA ATGTATTTAG
     3781 AAAAATAAAC AAATAGGGGT TCCGCGCACC TGCACCAGTC AGTAAAACGA CGGCCAGTAG
     3841 TCAAAAGCCT CCGACCGGAG GCTTTTGACT TGGTTCAGGT GGAGTGGGAG
//