LOCUS Exported 3482 bp DNA circular SYN 24-JUL-2020 DEFINITION synthetic linear DNA ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2001) AUTHORS Keoni Gandall TITLE Direct Submission JOURNAL Exported from Poly. FEATURES Location/Qualifiers terminator 1..3482 /label="T7 terminator" /note="transcription terminator for bacteriophage T7 RNA polymerase" terminator 38..85 /label="T7 terminator" /note="transcription terminator for bacteriophage T7 RNA polymerase" primer_bind 1933..1949 /label="M13 for" /note="common sequencing primer, one of multiple similar variants" terminator 1950..1981 /label="tonB terminator" /note="bidirectional E. coli tonB-P14 transcription terminator" CDS 2030..3388 /label="murF" /note="Involved in cell wall formation. Catalyzes the final step in the synthesis of UDP-N-acetylmuramoyl-pentapeptide, the precursor of murein." /translation="MISVTLSQLTDILNGELQGADITLDAVTTDTRKLTPGCLFVALKGERFDAHDFADQAKAGGAGALLVSRPLDIDLPQLIVKDTRLAFGELAAWVRQQVPARVVALTGSSGKTSVKEMTAAILSQCGNTLYTAGNLNNDIGVPMTLLRLTPEYDYAVIELGANHQGEIAWTVSLTRPEAALVNNLAAAHLEGFGSLAGVAKAKGEIFSGLPENGIAIMNADNNDWLNWQSVIGSRKVWRFSPNAANSDFTATNIHVTSHGTEFTLQTPTGSVDVLLPLPGRHNIANALAAAALSMSVGATLDAIKAGLANLKAVPGRLFPIQLAENQLLLDDSYNANVGSMTAAVQVLAEMPGYRVLVVGDMAELGAESEACHVQVGEAAKAAGIDRVLSVGKQSHAISTASGVGEHFADKTALITRLKLLIAEQQVITILVKGSRSAAMEEVVRALQENGTC" primer_bind complement(90..106) /label="M13 rev" /note="common sequencing primer, one of multiple similar variants" rep_origin complement(173..761) /label="ori" /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(952..1812) /label="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*" promoter complement(1813..1917) /label="bla promoter" /note="ampicillin resistance gene promoter" misc_feature 3423..3482 /label="FreeGenes Barcode" /note="FreeGenes Barcode. Defined as I-SceI site flanked by 21 base pair random sequences." ORIGIN 1 CGCTGAGGTG TCAATCGTCG GAGCCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC 61 TAAACGGGTC TTGAGGGGTT TTTTGCATGG TCATAGCTGT TTCCTGAGAG CTTGGCAGGT 121 GATGACACAC ATTAACAAAT TTCGTGAGGA GTCTCCAGAA GAATGCCATT AATTTCCATA 181 GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC 241 CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG 301 TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC 361 TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG 421 GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC 481 TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA 541 TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG 601 GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA 661 AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG 721 TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGGCCTA CTATTAGCAA 781 CAACGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA 841 AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA 901 ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAAACT TGGTCTGACA GTTACCAATG 961 CTTAATCAGT GAGGCACCTA TCTCAGCGAT CTGTCTATTT CGTTCATCCA TAGTTGCCTG 1021 ACTCCCCGTC GTGTAGATAA CTACGATACG GGAGGGCTTA CCATCTGGCC CCAGTGCTGC 1081 AATGATACCG CGAGAACCAC GCTCACCGGC TCCAGATTTA TCAGCAATAA ACCAGCCAGC 1141 CGGAAGGGCC GAGCGCAGAA GTGGTCCTGC AACTTTATCC GCCTCCATCC AGTCTATTAA 1201 TTGTTGCCGG GAAGCTAGAG TAAGTAGTTC GCCAGTTAAT AGTTTGCGCA ACGTTGTTGC 1261 CATTGCTACA GGCATCGTGG TGTCACGCTC GTCGTTTGGT ATGGCTTCAT TCAGCTCCGG 1321 TTCCCAACGA TCAAGGCGAG TTACATGATC CCCCATGTTG TGCAAAAAAG CGGTTAGCTC 1381 CTTCGGTCCT CCGATCGTTG TCAGAAGTAA GTTGGCCGCA GTGTTATCAC TCATGGTTAT 1441 GGCAGCACTG CATAATTCTC TTACTGTCAT GCCATCCGTA AGATGCTTTT CTGTGACTGG 1501 TGAGTACTCA ACCAAGTCAT TCTGAGAATA GTGTATGCGG CGACCGAGTT GCTCTTGCCC 1561 GGCGTCAATA CGGGATAATA CCGCGCCACA TAGCAGAACT TTAAAAGTGC TCATCATTGG 1621 AAAACGTTCT TCGGGGCGAA AACTCTCAAG GATCTTACCG CTGTTGAGAT CCAGTTCGAT 1681 GTAACCCACT CGTGCACCCA ACTGATCTTC AGCATCTTTT ACTTTCACCA GCGTTTCTGG 1741 GTGAGCAAAA ACAGGAAGGC AAAATGCCGC AAAAAAGGGA ATAAGGGCGA CACGGAAATG 1801 TTGAATACTC ATACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT 1861 CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC 1921 CTGCACCAGT CAGTAAAACG ACGGCCAGTA GTCAAAAGCC TCCGACCGGA GGCTTTTGAC 1981 TTGGTTCAGG TGGAGTGGGA GAAACACGTG GCAAACATTC CGGTCTCTAA TGATCTCAGT 2041 CACCCTATCA CAGCTAACCG ACATCCTAAA CGGAGAACTA CAGGGAGCAG ACATCACCCT 2101 AGACGCAGTC ACCACCGACA CCCGTAAACT AACCCCGGGA TGCCTATTCG TCGCACTAAA 2161 AGGAGAACGT TTCGACGCAC ACGACTTCGC AGACCAGGCA AAAGCAGGAG GAGCAGGAGC 2221 ACTACTAGTC TCACGTCCGC TAGACATCGA CCTACCGCAG CTAATCGTCA AAGACACCCG 2281 TCTAGCATTC GGAGAACTAG CAGCATGGGT CCGTCAGCAG GTCCCGGCAC GTGTCGTCGC 2341 ACTAACCGGA TCATCAGGAA AAACCTCAGT CAAAGAAATG ACCGCAGCAA TCCTATCACA 2401 GTGCGGAAAC ACCCTATACA CCGCAGGAAA CCTAAACAAC GACATCGGAG TCCCGATGAC 2461 CCTACTACGT CTAACCCCGG AATACGACTA CGCAGTCATC GAACTAGGAG CAAACCACCA 2521 GGGAGAAATC GCATGGACCG TCTCACTAAC CCGTCCGGAA GCAGCACTAG TCAACAACCT 2581 AGCAGCAGCA CACCTAGAAG GATTCGGATC ACTAGCAGGA GTCGCAAAAG CAAAAGGAGA 2641 AATCTTCTCA GGACTACCGG AAAACGGAAT CGCAATCATG AACGCAGACA ACAACGACTG 2701 GCTAAACTGG CAGTCAGTCA TCGGATCACG TAAAGTCTGG CGTTTCTCAC CGAACGCAGC 2761 AAACTCAGAC TTCACCGCAA CCAACATCCA CGTCACCTCA CACGGAACCG AATTCACCCT 2821 ACAGACCCCG ACCGGATCAG TCGACGTCCT ACTACCGCTA CCGGGACGTC ACAACATCGC 2881 AAACGCACTA GCAGCAGCAG CACTATCAAT GTCAGTCGGA GCAACCCTAG ACGCAATCAA 2941 AGCAGGACTA GCAAACCTAA AAGCAGTCCC GGGACGTCTA TTCCCGATCC AGCTAGCAGA 3001 AAACCAGCTA CTACTAGACG ACTCATACAA CGCAAACGTC GGATCAATGA CCGCAGCAGT 3061 CCAGGTCCTA GCAGAAATGC CGGGATACCG TGTCCTAGTC GTCGGAGACA TGGCAGAACT 3121 AGGAGCAGAA TCAGAAGCAT GCCACGTCCA GGTCGGAGAA GCAGCAAAAG CAGCAGGAAT 3181 CGACCGTGTC CTATCAGTCG GAAAACAGTC ACACGCAATC TCAACCGCAT CAGGAGTCGG 3241 AGAACACTTC GCAGACAAAA CCGCACTAAT CACCCGTCTA AAACTACTAA TCGCAGAACA 3301 GCAGGTCATC ACCATCCTAG TCAAAGGATC ACGTTCAGCA GCAATGGAAG AAGTCGTCCG 3361 TGCACTACAG GAAAACGGAA CCTGCTGAAG AGCTTCGAGA CCAAACCGGA GCCATACAGT 3421 ACGTCTCTAA AAAGGAGGTG ACGTAGGGAT AACAGGGTAA TAGTCGAAGA TTGATGGCTC 3481 TC //