LOCUS       Exported     3440 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..3440
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
                     /label="T7 terminator"
     terminator      1477..1524
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     3372..3388
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      3389..3420
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             29..1345
                     /label="murD"
                     /note="Cell wall formation. Catalyzes the addition of glutamate to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanine (UMA)."
                     /translation="MADYQGKNVVIIGLGLTGLSCVDFFLARGVTPRVMDTRMTPPGLDKLPEAVERHTGSLNDEWLMAADLIVASPGIALAHPSLSAAADAGIEIVGDIELFCREAQAPIVAITGSNGKSTVTTLVGEMAKAAGVNVGVGGNIGLPALMLLDDECELYVLELSSFQLETTSSLQAVAATILNVTEDHMDRYPFGLQQYRAAKLRIYENAKVCVVNADDALTMPIRGADERCVSFGVNMGDYHLNHQQGETWLRVKGEKVLNVKEMKLSGQHNYTNALAALALADAAGLPRASSLKALTTFTGLPHRFEVVLEHNGVRWINDSKATNVGSTEAALNGLHVDGTLHLLLGGDGKSADFSPLARYLNGDNVRLYCFGRDGAQLAALRPEVAEQTETMEQAMRLLAPRVQPGDMVLLSPACASLDQFKNFEQRGNEFARLAKELG"
     primer_bind     complement(1529..1545)
                     /note="common sequencing primer, one of multiple similar variants"
                     /label="M13 rev"
     rep_origin      complement(1612..2200)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(2391..3251)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(3252..3356)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
     misc_feature    1380..1439
                     /label="FreeGenes Barcode"
                     /note="FreeGenes Barcode. Defined as I-SceI site flanked by 21 base pair random sequences."
ORIGIN
        1 AAACACGTGG CAAACATTCC GGTCTCTAAT GGCAGACTAC CAGGGAAAAA ACGTCGTCAT
       61 CATCGGACTA GGACTAACCG GACTATCATG CGTCGACTTC TTCCTAGCAC GTGGAGTCAC
      121 CCCGCGTGTC ATGGACACCC GTATGACCCC GCCGGGACTA GACAAACTAC CGGAAGCAGT
      181 CGAACGTCAC ACCGGATCAC TAAACGACGA ATGGCTAATG GCAGCAGACC TAATCGTCGC
      241 ATCACCGGGA ATCGCACTAG CACACCCGTC ACTATCAGCA GCAGCAGACG CAGGAATCGA
      301 AATCGTCGGA GACATCGAAC TATTCTGCCG TGAAGCACAG GCACCGATCG TCGCAATCAC
      361 CGGATCAAAC GGAAAATCAA CCGTCACCAC CCTAGTCGGA GAAATGGCAA AAGCAGCAGG
      421 AGTCAACGTC GGAGTCGGAG GAAACATCGG ACTACCGGCA CTAATGCTAC TAGACGACGA
      481 ATGCGAACTA TACGTCCTAG AACTATCATC ATTCCAGCTA GAAACCACCT CATCACTACA
      541 GGCAGTCGCA GCAACCATCC TAAACGTCAC CGAAGACCAC ATGGACCGTT ACCCGTTCGG
      601 ACTACAGCAG TACCGTGCAG CAAAACTACG TATCTACGAA AACGCAAAAG TCTGCGTCGT
      661 CAACGCAGAC GACGCACTAA CCATGCCGAT CCGTGGAGCA GACGAACGTT GCGTCTCATT
      721 CGGAGTCAAC ATGGGAGACT ACCACCTAAA CCACCAGCAG GGAGAAACCT GGCTACGTGT
      781 CAAAGGAGAA AAAGTCCTAA ACGTCAAAGA AATGAAACTA TCAGGACAGC ACAACTACAC
      841 CAACGCACTA GCAGCACTAG CACTAGCAGA CGCAGCAGGA CTACCGCGTG CATCATCACT
      901 AAAAGCACTA ACCACCTTCA CCGGACTACC GCACCGTTTC GAAGTCGTCC TAGAACACAA
      961 CGGAGTCCGT TGGATCAACG ACTCAAAAGC AACCAACGTC GGATCAACCG AAGCAGCACT
     1021 AAACGGACTA CACGTCGACG GAACCCTACA CCTACTACTA GGAGGAGACG GAAAATCAGC
     1081 AGACTTCTCA CCGCTAGCAC GTTACCTAAA CGGAGACAAC GTCCGTCTAT ACTGCTTCGG
     1141 ACGTGACGGA GCACAGCTAG CAGCACTACG TCCGGAAGTC GCAGAACAGA CCGAAACCAT
     1201 GGAACAGGCA ATGCGTCTAC TAGCACCGCG TGTCCAGCCG GGAGACATGG TCCTACTATC
     1261 ACCGGCATGC GCATCACTAG ACCAGTTCAA AAACTTCGAA CAGCGTGGAA ACGAATTCGC
     1321 ACGTCTAGCA AAAGAACTAG GATGAAGAGC TTCGAGACCA CAAGGAGTCG GCATATCACG
     1381 ATTTGTAGTG CGACCTAGCT TAGGGATAAC AGGGTAATTA GTTGTCCTAT GAAGAAGTTC
     1441 GCTGAGGTGT CAATCGTCGG AGCCGCTGAG CAATAACTAG CATAACCCCT TGGGGCCTCT
     1501 AAACGGGTCT TGAGGGGTTT TTTGCATGGT CATAGCTGTT TCCTGAGAGC TTGGCAGGTG
     1561 ATGACACACA TTAACAAATT TCGTGAGGAG TCTCCAGAAG AATGCCATTA ATTTCCATAG
     1621 GCTCCGCCCC CCTGACGAGC ATCACAAAAA TCGACGCTCA AGTCAGAGGT GGCGAAACCC
     1681 GACAGGACTA TAAAGATACC AGGCGTTTCC CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT
     1741 TCCGACCCTG CCGCTTACCG GATACCTGTC CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT
     1801 TTCTCATAGC TCACGCTGTA GGTATCTCAG TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG
     1861 CTGTGTGCAC GAACCCCCCG TTCAGCCCGA CCGCTGCGCC TTATCCGGTA ACTATCGTCT
     1921 TGAGTCCAAC CCGGTAAGAC ACGACTTATC GCCACTGGCA GCAGCCACTG GTAACAGGAT
     1981 TAGCAGAGCG AGGTATGTAG GCGGTGCTAC AGAGTTCTTG AAGTGGTGGC CTAACTACGG
     2041 CTACACTAGA AGAACAGTAT TTGGTATCTG CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA
     2101 AAGAGTTGGT AGCTCTTGAT CCGGCAAACA AACCACCGCT GGTAGCGGTG GTTTTTTTGT
     2161 TTGCAAGCAG CAGATTACGC GCAGAAAAAA AGGATCTCAA GAAGGCCTAC TATTAGCAAC
     2221 AACGATCCTT TGATCTTTTC TACGGGGTCT GACGCTCAGT GGAACGAAAA CTCACGTTAA
     2281 GGGATTTTGG TCATGAGATT ATCAAAAAGG ATCTTCACCT AGATCCTTTT AAATTAAAAA
     2341 TGAAGTTTTA AATCAATCTA AAGTATATAT GAGTAAACTT GGTCTGACAG TTACCAATGC
     2401 TTAATCAGTG AGGCACCTAT CTCAGCGATC TGTCTATTTC GTTCATCCAT AGTTGCCTGA
     2461 CTCCCCGTCG TGTAGATAAC TACGATACGG GAGGGCTTAC CATCTGGCCC CAGTGCTGCA
     2521 ATGATACCGC GAGAACCACG CTCACCGGCT CCAGATTTAT CAGCAATAAA CCAGCCAGCC
     2581 GGAAGGGCCG AGCGCAGAAG TGGTCCTGCA ACTTTATCCG CCTCCATCCA GTCTATTAAT
     2641 TGTTGCCGGG AAGCTAGAGT AAGTAGTTCG CCAGTTAATA GTTTGCGCAA CGTTGTTGCC
     2701 ATTGCTACAG GCATCGTGGT GTCACGCTCG TCGTTTGGTA TGGCTTCATT CAGCTCCGGT
     2761 TCCCAACGAT CAAGGCGAGT TACATGATCC CCCATGTTGT GCAAAAAAGC GGTTAGCTCC
     2821 TTCGGTCCTC CGATCGTTGT CAGAAGTAAG TTGGCCGCAG TGTTATCACT CATGGTTATG
     2881 GCAGCACTGC ATAATTCTCT TACTGTCATG CCATCCGTAA GATGCTTTTC TGTGACTGGT
     2941 GAGTACTCAA CCAAGTCATT CTGAGAATAG TGTATGCGGC GACCGAGTTG CTCTTGCCCG
     3001 GCGTCAATAC GGGATAATAC CGCGCCACAT AGCAGAACTT TAAAAGTGCT CATCATTGGA
     3061 AAACGTTCTT CGGGGCGAAA ACTCTCAAGG ATCTTACCGC TGTTGAGATC CAGTTCGATG
     3121 TAACCCACTC GTGCACCCAA CTGATCTTCA GCATCTTTTA CTTTCACCAG CGTTTCTGGG
     3181 TGAGCAAAAA CAGGAAGGCA AAATGCCGCA AAAAAGGGAA TAAGGGCGAC ACGGAAATGT
     3241 TGAATACTCA TACTCTTCCT TTTTCAATAT TATTGAAGCA TTTATCAGGG TTATTGTCTC
     3301 ATGAGCGGAT ACATATTTGA ATGTATTTAG AAAAATAAAC AAATAGGGGT TCCGCGCACC
     3361 TGCACCAGTC AGTAAAACGA CGGCCAGTAG TCAAAAGCCT CCGACCGGAG GCTTTTGACT
     3421 TGGTTCAGGT GGAGTGGGAG
//