LOCUS Exported 4829 bp DNA circular SYN 24-JUL-2020 DEFINITION synthetic linear DNA ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2001) AUTHORS Keoni Gandall TITLE Direct Submission JOURNAL Exported from Poly. FEATURES Location/Qualifiers terminator 1..4829 /label="T7 terminator" /note="transcription terminator for bacteriophage T7 RNA polymerase" terminator 38..85 /note="transcription terminator for bacteriophage T7 RNA polymerase" /label="T7 terminator" primer_bind 1933..1949 /label="M13 for" /note="common sequencing primer, one of multiple similar variants" terminator 1950..1981 /label="tonB terminator" /note="bidirectional E. coli tonB-P14 transcription terminator" CDS 2030..4735 /translation="MLIKLLTKVFGSRNDRTLRRMRKVVNIINAMEPEMEKLSDEELKGKTAEFRARLEKGEVLENLIPEAFAVVREASKRVFGMRHFDVQLLGGMVLNERCIAEMRTGEGKTLTATLPAYLNALTGKGVHVVTVNDYLAQRDAENNRPLFEFLGLTVGINLPGMPAPAKREAYAADITYGTNNEYGFDYLRDNMAFSPEERVQRKLHYALVDEVDSILIDEARTPLIISGPAEDSSEMYKRVNKIIPHLIRQEKEDSETFQGEGHFSVDEKSRQVNLTERGLVLIEELLVKEGIMDEGESLYSPANIMLMHHVTAALRAHALFTRDVDYIVKDGEVIIVDEHTGRTMQGRRWSDGLHQAVEAKEGVQIQNENQTLASITFQNYFRLYEKLAGMTGTADTEAFEFSSIYKLDTVVVPTNRPMIRKDLPDLVYMTEAEKIQAIIEDIKERTAKGQPVLVGTISIEKSELVSNELTKAGIKHNVLNAKFHANEAAIVAQAGYPAAVTIATNMAGRGTDIVLGGSWQAEVAALENPTAEQIEKIKADWQVRHDAVLEAGGLHIIGTERHESRRIDNQLRGRSGRQGDAGSSRFYLSMEDALMRIFASDRVSGMMRKLGMKPGEAIEHPWVTKAIANAQRKVESRNFDIRKQLLEYDDVANDQRRAIYSQRNELLDVSDVSETINSIREDVFKATIDAYIPPQSLEEMWDIPGLQERLKNDFDLDLPIAEWLDKEPELHEETLRERILAQSIEVYQRKEEVVGAEMMRHFEKGVMLQTLDSLWKEHLAAMDYLRQGIHLRGYAQKDPKQEYKRESFSMFAAMLESLKYEVISTLSKVQVRMPEEVEELEQQRRMEAERLAQMQQLSHQDDDSAAAAALAAQTGERKVGRNDPCPCGSGKKYKQCHGRLQ" /label="secA" /note="Required for protein export, interacts with the SecYEG preprotein conducting channel. SecA has a central role in coupling the hydrolysis of ATP to the transfer of proteins into and across the cell membrane, serving both as a receptor for the preprotein-SecB complex and as an ATP-driven molecular motor driving the stepwise translocation of polypeptide chains across the membrane." primer_bind complement(90..106) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" rep_origin complement(173..761) /label="ori" /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(952..1812) /label="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*" promoter complement(1813..1917) /label="bla promoter" /note="ampicillin resistance gene promoter" misc_feature 4770..4829 /label="FreeGenes Barcode" /note="FreeGenes Barcode. Defined as I-SceI site flanked by 21 base pair random sequences." ORIGIN 1 CGCTGAGGTG TCAATCGTCG GAGCCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC 61 TAAACGGGTC TTGAGGGGTT TTTTGCATGG TCATAGCTGT TTCCTGAGAG CTTGGCAGGT 121 GATGACACAC ATTAACAAAT TTCGTGAGGA GTCTCCAGAA GAATGCCATT AATTTCCATA 181 GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC 241 CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG 301 TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC 361 TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG 421 GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC 481 TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA 541 TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG 601 GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA 661 AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG 721 TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGGCCTA CTATTAGCAA 781 CAACGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA 841 AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA 901 ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAAACT TGGTCTGACA GTTACCAATG 961 CTTAATCAGT GAGGCACCTA TCTCAGCGAT CTGTCTATTT CGTTCATCCA TAGTTGCCTG 1021 ACTCCCCGTC GTGTAGATAA CTACGATACG GGAGGGCTTA CCATCTGGCC CCAGTGCTGC 1081 AATGATACCG CGAGAACCAC GCTCACCGGC TCCAGATTTA TCAGCAATAA ACCAGCCAGC 1141 CGGAAGGGCC GAGCGCAGAA GTGGTCCTGC AACTTTATCC GCCTCCATCC AGTCTATTAA 1201 TTGTTGCCGG GAAGCTAGAG TAAGTAGTTC GCCAGTTAAT AGTTTGCGCA ACGTTGTTGC 1261 CATTGCTACA GGCATCGTGG TGTCACGCTC GTCGTTTGGT ATGGCTTCAT TCAGCTCCGG 1321 TTCCCAACGA TCAAGGCGAG TTACATGATC CCCCATGTTG TGCAAAAAAG CGGTTAGCTC 1381 CTTCGGTCCT CCGATCGTTG TCAGAAGTAA GTTGGCCGCA GTGTTATCAC TCATGGTTAT 1441 GGCAGCACTG CATAATTCTC TTACTGTCAT GCCATCCGTA AGATGCTTTT CTGTGACTGG 1501 TGAGTACTCA ACCAAGTCAT TCTGAGAATA GTGTATGCGG CGACCGAGTT GCTCTTGCCC 1561 GGCGTCAATA CGGGATAATA CCGCGCCACA TAGCAGAACT TTAAAAGTGC TCATCATTGG 1621 AAAACGTTCT TCGGGGCGAA AACTCTCAAG GATCTTACCG CTGTTGAGAT CCAGTTCGAT 1681 GTAACCCACT CGTGCACCCA ACTGATCTTC AGCATCTTTT ACTTTCACCA GCGTTTCTGG 1741 GTGAGCAAAA ACAGGAAGGC AAAATGCCGC AAAAAAGGGA ATAAGGGCGA CACGGAAATG 1801 TTGAATACTC ATACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT 1861 CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC 1921 CTGCACCAGT CAGTAAAACG ACGGCCAGTA GTCAAAAGCC TCCGACCGGA GGCTTTTGAC 1981 TTGGTTCAGG TGGAGTGGGA GAAACCGGAG CCATACAGTA CGGTCTCTAA TGCTAATCAA 2041 ACTACTAACC AAAGTCTTCG GATCACGTAA CGACCGTACC CTACGTCGTA TGCGTAAAGT 2101 CGTCAACATC ATCAACGCAA TGGAACCGGA AATGGAAAAA CTATCAGACG AAGAACTAAA 2161 AGGAAAAACC GCAGAATTCC GTGCACGTCT AGAAAAAGGA GAAGTCCTAG AAAACCTAAT 2221 CCCGGAAGCA TTCGCAGTCG TCCGTGAAGC ATCAAAACGT GTCTTCGGAA TGCGTCACTT 2281 CGACGTCCAG CTACTAGGAG GAATGGTCCT AAACGAACGT TGCATCGCAG AAATGCGTAC 2341 CGGAGAAGGA AAAACCCTAA CCGCAACCCT ACCGGCATAC CTAAACGCAC TAACCGGAAA 2401 AGGAGTCCAC GTCGTCACCG TCAACGACTA CCTAGCACAG CGTGACGCAG AAAACAACCG 2461 TCCGCTATTC GAATTCCTAG GACTAACCGT CGGAATCAAC CTACCGGGAA TGCCGGCACC 2521 GGCAAAACGT GAAGCATACG CAGCAGACAT CACCTACGGA ACCAACAACG AATACGGATT 2581 CGACTACCTA CGTGACAACA TGGCATTCTC ACCGGAAGAA CGTGTCCAGC GTAAACTACA 2641 CTACGCACTA GTCGACGAAG TCGACTCAAT CCTAATCGAC GAAGCACGTA CCCCGCTAAT 2701 CATCTCAGGA CCGGCAGAAG ACTCATCAGA AATGTACAAA CGTGTCAACA AAATCATCCC 2761 GCACCTAATC CGTCAGGAAA AAGAAGACTC AGAAACCTTC CAGGGAGAAG GACACTTCTC 2821 AGTCGACGAA AAATCACGTC AGGTCAACCT AACCGAACGT GGACTAGTCC TAATCGAAGA 2881 ACTACTAGTC AAAGAAGGAA TCATGGACGA AGGAGAATCA CTATACTCAC CGGCAAACAT 2941 CATGCTAATG CACCACGTCA CCGCAGCACT ACGTGCACAC GCACTATTCA CCCGTGACGT 3001 CGACTACATC GTCAAAGACG GAGAAGTCAT CATCGTCGAC GAACACACCG GACGTACCAT 3061 GCAGGGACGT CGTTGGTCAG ACGGACTACA CCAGGCAGTC GAAGCAAAAG AAGGAGTCCA 3121 GATCCAGAAC GAAAACCAGA CCCTAGCATC AATCACCTTC CAGAACTACT TCCGTCTATA 3181 CGAAAAACTA GCAGGAATGA CCGGAACCGC AGACACCGAA GCATTCGAAT TCTCATCAAT 3241 CTACAAACTA GACACCGTCG TCGTCCCGAC CAACCGTCCG ATGATCCGTA AAGACCTACC 3301 GGACCTAGTC TACATGACCG AAGCAGAAAA AATCCAGGCA ATCATCGAAG ACATCAAAGA 3361 ACGTACCGCA AAAGGACAGC CGGTCCTAGT CGGAACCATC TCAATCGAAA AATCAGAACT 3421 AGTCTCAAAC GAACTAACCA AAGCAGGAAT CAAACACAAC GTCCTAAACG CAAAATTCCA 3481 CGCAAACGAA GCAGCAATCG TCGCACAGGC AGGATACCCG GCAGCAGTCA CCATCGCAAC 3541 CAACATGGCA GGACGTGGAA CCGACATCGT CCTAGGAGGA TCATGGCAGG CAGAAGTCGC 3601 AGCACTAGAA AACCCGACCG CAGAACAGAT CGAAAAAATC AAAGCAGACT GGCAGGTCCG 3661 TCACGACGCA GTCCTAGAAG CAGGAGGACT ACACATCATC GGAACCGAAC GTCACGAATC 3721 ACGTCGTATC GACAACCAGC TACGTGGACG TTCAGGACGT CAGGGAGACG CAGGATCATC 3781 ACGTTTCTAC CTATCAATGG AAGACGCACT AATGCGTATC TTCGCATCAG ACCGTGTCTC 3841 AGGAATGATG CGTAAACTAG GAATGAAACC GGGAGAAGCA ATCGAACACC CGTGGGTCAC 3901 CAAAGCAATC GCAAACGCAC AGCGTAAAGT CGAATCACGT AACTTCGACA TCCGTAAACA 3961 GCTACTAGAA TACGACGACG TCGCAAACGA CCAGCGTCGT GCAATCTACT CACAGCGTAA 4021 CGAACTACTA GACGTCTCAG ACGTCTCAGA AACCATCAAC TCAATCCGTG AAGACGTCTT 4081 CAAAGCAACC ATCGACGCAT ACATCCCGCC GCAGTCACTA GAAGAAATGT GGGACATCCC 4141 GGGACTACAG GAACGTCTAA AAAACGACTT CGACCTAGAC CTACCGATCG CAGAATGGCT 4201 AGACAAAGAA CCGGAACTAC ACGAAGAAAC CCTACGTGAA CGTATCCTAG CACAGTCAAT 4261 CGAAGTCTAC CAGCGTAAAG AAGAAGTCGT CGGAGCAGAA ATGATGCGTC ACTTCGAAAA 4321 AGGAGTCATG CTACAGACCC TAGACTCACT ATGGAAAGAA CACCTAGCAG CAATGGACTA 4381 CCTACGTCAG GGAATCCACC TACGTGGATA CGCACAGAAA GACCCGAAAC AGGAATACAA 4441 ACGTGAATCA TTCTCAATGT TCGCAGCAAT GCTAGAATCA CTAAAATACG AAGTCATCTC 4501 AACCCTATCA AAAGTCCAGG TCCGTATGCC GGAAGAAGTC GAAGAACTAG AACAGCAGCG 4561 TCGTATGGAA GCAGAACGTC TAGCACAGAT GCAGCAGCTA TCACACCAGG ACGACGACTC 4621 AGCAGCAGCA GCAGCACTAG CAGCACAGAC CGGAGAACGT AAAGTCGGAC GTAACGACCC 4681 GTGCCCGTGC GGATCAGGAA AAAAATACAA ACAGTGCCAC GGACGTCTAC AGTGAAGAGC 4741 TTCGAGACCA AGGAACTATG GCATCGAGCG CGTGGTAAGG AGGATACAGC TAGGGATAAC 4801 AGGGTAATGG TCGGCTGTTA GTGCCTGGT //