LOCUS       Exported     4829 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..4829
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      38..85
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
                     /label="T7 terminator"
     primer_bind     1933..1949
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      1950..1981
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             2030..4735
                     /translation="MLIKLLTKVFGSRNDRTLRRMRKVVNIINAMEPEMEKLSDEELKGKTAEFRARLEKGEVLENLIPEAFAVVREASKRVFGMRHFDVQLLGGMVLNERCIAEMRTGEGKTLTATLPAYLNALTGKGVHVVTVNDYLAQRDAENNRPLFEFLGLTVGINLPGMPAPAKREAYAADITYGTNNEYGFDYLRDNMAFSPEERVQRKLHYALVDEVDSILIDEARTPLIISGPAEDSSEMYKRVNKIIPHLIRQEKEDSETFQGEGHFSVDEKSRQVNLTERGLVLIEELLVKEGIMDEGESLYSPANIMLMHHVTAALRAHALFTRDVDYIVKDGEVIIVDEHTGRTMQGRRWSDGLHQAVEAKEGVQIQNENQTLASITFQNYFRLYEKLAGMTGTADTEAFEFSSIYKLDTVVVPTNRPMIRKDLPDLVYMTEAEKIQAIIEDIKERTAKGQPVLVGTISIEKSELVSNELTKAGIKHNVLNAKFHANEAAIVAQAGYPAAVTIATNMAGRGTDIVLGGSWQAEVAALENPTAEQIEKIKADWQVRHDAVLEAGGLHIIGTERHESRRIDNQLRGRSGRQGDAGSSRFYLSMEDALMRIFASDRVSGMMRKLGMKPGEAIEHPWVTKAIANAQRKVESRNFDIRKQLLEYDDVANDQRRAIYSQRNELLDVSDVSETINSIREDVFKATIDAYIPPQSLEEMWDIPGLQERLKNDFDLDLPIAEWLDKEPELHEETLRERILAQSIEVYQRKEEVVGAEMMRHFEKGVMLQTLDSLWKEHLAAMDYLRQGIHLRGYAQKDPKQEYKRESFSMFAAMLESLKYEVISTLSKVQVRMPEEVEELEQQRRMEAERLAQMQQLSHQDDDSAAAAALAAQTGERKVGRNDPCPCGSGKKYKQCHGRLQ"
                     /label="secA"
                     /note="Required for protein export, interacts with the SecYEG preprotein conducting channel. SecA has a central role in coupling the hydrolysis of ATP to the transfer of proteins into and across the cell membrane, serving both as a receptor for the preprotein-SecB complex and as an ATP-driven molecular motor driving the stepwise translocation of polypeptide chains across the membrane."
     primer_bind     complement(90..106)
                     /note="common sequencing primer, one of multiple similar variants"
                     /label="M13 rev"
     rep_origin      complement(173..761)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(952..1812)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(1813..1917)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
     misc_feature    4770..4829
                     /label="FreeGenes Barcode"
                     /note="FreeGenes Barcode. Defined as I-SceI site flanked by 21 base pair random sequences."
ORIGIN
        1 CGCTGAGGTG TCAATCGTCG GAGCCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC
       61 TAAACGGGTC TTGAGGGGTT TTTTGCATGG TCATAGCTGT TTCCTGAGAG CTTGGCAGGT
      121 GATGACACAC ATTAACAAAT TTCGTGAGGA GTCTCCAGAA GAATGCCATT AATTTCCATA
      181 GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC
      241 CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG
      301 TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC
      361 TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG
      421 GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC
      481 TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA
      541 TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG
      601 GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA
      661 AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG
      721 TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGGCCTA CTATTAGCAA
      781 CAACGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA
      841 AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA
      901 ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAAACT TGGTCTGACA GTTACCAATG
      961 CTTAATCAGT GAGGCACCTA TCTCAGCGAT CTGTCTATTT CGTTCATCCA TAGTTGCCTG
     1021 ACTCCCCGTC GTGTAGATAA CTACGATACG GGAGGGCTTA CCATCTGGCC CCAGTGCTGC
     1081 AATGATACCG CGAGAACCAC GCTCACCGGC TCCAGATTTA TCAGCAATAA ACCAGCCAGC
     1141 CGGAAGGGCC GAGCGCAGAA GTGGTCCTGC AACTTTATCC GCCTCCATCC AGTCTATTAA
     1201 TTGTTGCCGG GAAGCTAGAG TAAGTAGTTC GCCAGTTAAT AGTTTGCGCA ACGTTGTTGC
     1261 CATTGCTACA GGCATCGTGG TGTCACGCTC GTCGTTTGGT ATGGCTTCAT TCAGCTCCGG
     1321 TTCCCAACGA TCAAGGCGAG TTACATGATC CCCCATGTTG TGCAAAAAAG CGGTTAGCTC
     1381 CTTCGGTCCT CCGATCGTTG TCAGAAGTAA GTTGGCCGCA GTGTTATCAC TCATGGTTAT
     1441 GGCAGCACTG CATAATTCTC TTACTGTCAT GCCATCCGTA AGATGCTTTT CTGTGACTGG
     1501 TGAGTACTCA ACCAAGTCAT TCTGAGAATA GTGTATGCGG CGACCGAGTT GCTCTTGCCC
     1561 GGCGTCAATA CGGGATAATA CCGCGCCACA TAGCAGAACT TTAAAAGTGC TCATCATTGG
     1621 AAAACGTTCT TCGGGGCGAA AACTCTCAAG GATCTTACCG CTGTTGAGAT CCAGTTCGAT
     1681 GTAACCCACT CGTGCACCCA ACTGATCTTC AGCATCTTTT ACTTTCACCA GCGTTTCTGG
     1741 GTGAGCAAAA ACAGGAAGGC AAAATGCCGC AAAAAAGGGA ATAAGGGCGA CACGGAAATG
     1801 TTGAATACTC ATACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT
     1861 CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC
     1921 CTGCACCAGT CAGTAAAACG ACGGCCAGTA GTCAAAAGCC TCCGACCGGA GGCTTTTGAC
     1981 TTGGTTCAGG TGGAGTGGGA GAAACCGGAG CCATACAGTA CGGTCTCTAA TGCTAATCAA
     2041 ACTACTAACC AAAGTCTTCG GATCACGTAA CGACCGTACC CTACGTCGTA TGCGTAAAGT
     2101 CGTCAACATC ATCAACGCAA TGGAACCGGA AATGGAAAAA CTATCAGACG AAGAACTAAA
     2161 AGGAAAAACC GCAGAATTCC GTGCACGTCT AGAAAAAGGA GAAGTCCTAG AAAACCTAAT
     2221 CCCGGAAGCA TTCGCAGTCG TCCGTGAAGC ATCAAAACGT GTCTTCGGAA TGCGTCACTT
     2281 CGACGTCCAG CTACTAGGAG GAATGGTCCT AAACGAACGT TGCATCGCAG AAATGCGTAC
     2341 CGGAGAAGGA AAAACCCTAA CCGCAACCCT ACCGGCATAC CTAAACGCAC TAACCGGAAA
     2401 AGGAGTCCAC GTCGTCACCG TCAACGACTA CCTAGCACAG CGTGACGCAG AAAACAACCG
     2461 TCCGCTATTC GAATTCCTAG GACTAACCGT CGGAATCAAC CTACCGGGAA TGCCGGCACC
     2521 GGCAAAACGT GAAGCATACG CAGCAGACAT CACCTACGGA ACCAACAACG AATACGGATT
     2581 CGACTACCTA CGTGACAACA TGGCATTCTC ACCGGAAGAA CGTGTCCAGC GTAAACTACA
     2641 CTACGCACTA GTCGACGAAG TCGACTCAAT CCTAATCGAC GAAGCACGTA CCCCGCTAAT
     2701 CATCTCAGGA CCGGCAGAAG ACTCATCAGA AATGTACAAA CGTGTCAACA AAATCATCCC
     2761 GCACCTAATC CGTCAGGAAA AAGAAGACTC AGAAACCTTC CAGGGAGAAG GACACTTCTC
     2821 AGTCGACGAA AAATCACGTC AGGTCAACCT AACCGAACGT GGACTAGTCC TAATCGAAGA
     2881 ACTACTAGTC AAAGAAGGAA TCATGGACGA AGGAGAATCA CTATACTCAC CGGCAAACAT
     2941 CATGCTAATG CACCACGTCA CCGCAGCACT ACGTGCACAC GCACTATTCA CCCGTGACGT
     3001 CGACTACATC GTCAAAGACG GAGAAGTCAT CATCGTCGAC GAACACACCG GACGTACCAT
     3061 GCAGGGACGT CGTTGGTCAG ACGGACTACA CCAGGCAGTC GAAGCAAAAG AAGGAGTCCA
     3121 GATCCAGAAC GAAAACCAGA CCCTAGCATC AATCACCTTC CAGAACTACT TCCGTCTATA
     3181 CGAAAAACTA GCAGGAATGA CCGGAACCGC AGACACCGAA GCATTCGAAT TCTCATCAAT
     3241 CTACAAACTA GACACCGTCG TCGTCCCGAC CAACCGTCCG ATGATCCGTA AAGACCTACC
     3301 GGACCTAGTC TACATGACCG AAGCAGAAAA AATCCAGGCA ATCATCGAAG ACATCAAAGA
     3361 ACGTACCGCA AAAGGACAGC CGGTCCTAGT CGGAACCATC TCAATCGAAA AATCAGAACT
     3421 AGTCTCAAAC GAACTAACCA AAGCAGGAAT CAAACACAAC GTCCTAAACG CAAAATTCCA
     3481 CGCAAACGAA GCAGCAATCG TCGCACAGGC AGGATACCCG GCAGCAGTCA CCATCGCAAC
     3541 CAACATGGCA GGACGTGGAA CCGACATCGT CCTAGGAGGA TCATGGCAGG CAGAAGTCGC
     3601 AGCACTAGAA AACCCGACCG CAGAACAGAT CGAAAAAATC AAAGCAGACT GGCAGGTCCG
     3661 TCACGACGCA GTCCTAGAAG CAGGAGGACT ACACATCATC GGAACCGAAC GTCACGAATC
     3721 ACGTCGTATC GACAACCAGC TACGTGGACG TTCAGGACGT CAGGGAGACG CAGGATCATC
     3781 ACGTTTCTAC CTATCAATGG AAGACGCACT AATGCGTATC TTCGCATCAG ACCGTGTCTC
     3841 AGGAATGATG CGTAAACTAG GAATGAAACC GGGAGAAGCA ATCGAACACC CGTGGGTCAC
     3901 CAAAGCAATC GCAAACGCAC AGCGTAAAGT CGAATCACGT AACTTCGACA TCCGTAAACA
     3961 GCTACTAGAA TACGACGACG TCGCAAACGA CCAGCGTCGT GCAATCTACT CACAGCGTAA
     4021 CGAACTACTA GACGTCTCAG ACGTCTCAGA AACCATCAAC TCAATCCGTG AAGACGTCTT
     4081 CAAAGCAACC ATCGACGCAT ACATCCCGCC GCAGTCACTA GAAGAAATGT GGGACATCCC
     4141 GGGACTACAG GAACGTCTAA AAAACGACTT CGACCTAGAC CTACCGATCG CAGAATGGCT
     4201 AGACAAAGAA CCGGAACTAC ACGAAGAAAC CCTACGTGAA CGTATCCTAG CACAGTCAAT
     4261 CGAAGTCTAC CAGCGTAAAG AAGAAGTCGT CGGAGCAGAA ATGATGCGTC ACTTCGAAAA
     4321 AGGAGTCATG CTACAGACCC TAGACTCACT ATGGAAAGAA CACCTAGCAG CAATGGACTA
     4381 CCTACGTCAG GGAATCCACC TACGTGGATA CGCACAGAAA GACCCGAAAC AGGAATACAA
     4441 ACGTGAATCA TTCTCAATGT TCGCAGCAAT GCTAGAATCA CTAAAATACG AAGTCATCTC
     4501 AACCCTATCA AAAGTCCAGG TCCGTATGCC GGAAGAAGTC GAAGAACTAG AACAGCAGCG
     4561 TCGTATGGAA GCAGAACGTC TAGCACAGAT GCAGCAGCTA TCACACCAGG ACGACGACTC
     4621 AGCAGCAGCA GCAGCACTAG CAGCACAGAC CGGAGAACGT AAAGTCGGAC GTAACGACCC
     4681 GTGCCCGTGC GGATCAGGAA AAAAATACAA ACAGTGCCAC GGACGTCTAC AGTGAAGAGC
     4741 TTCGAGACCA AGGAACTATG GCATCGAGCG CGTGGTAAGG AGGATACAGC TAGGGATAAC
     4801 AGGGTAATGG TCGGCTGTTA GTGCCTGGT
//