LOCUS Exported 4556 bp DNA circular SYN 24-JUL-2020 DEFINITION synthetic linear DNA ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2001) AUTHORS Keoni Gandall TITLE Direct Submission JOURNAL Exported from Poly. FEATURES Location/Qualifiers terminator 1..4556 /label="T7 terminator" /note="transcription terminator for bacteriophage T7 RNA polymerase" terminator 38..85 /label="T7 terminator" /note="transcription terminator for bacteriophage T7 RNA polymerase" primer_bind 1933..1949 /note="common sequencing primer, one of multiple similar variants" /label="M13 for" terminator 1950..1981 /label="tonB terminator" /note="bidirectional E. coli tonB-P14 transcription terminator" CDS 100..4555 /note="(Microbial infection) Acts as a receptor for CdiA-EC93, the contact-dependent growth inhibition (CDI) effector of E.coli strain EC93; antibodies against extracellular epitopes decrease CDI. Its role in CDI is independent of the other Bam complex components (PubMed:18761695). Is not the receptor for CdiA from E.coli strain 536 / UPEC, which does not have the same mode of toxicity as CdiA from strain EC93; the decreased expression of bamA101 in some experiments decreases the level of outer membrane proteins in general (PubMed:23469034, PubMed:23882017). Susceptibility to CdiA-EC93 is dependent on E.coli BamA; replacing BamA with the gene from S.typhimurium LT2, E.cloacae ATCC 13047 or D.dadantii 3937 renders cells resistant to CdiA-EC93. Cells with BamA from another bacteria no longer form CdiA-EC93-induced aggregates with EC93 cells. A chimera in which E.cloacae extracellular loops 6 and 7 are replaced with loops 6 and 7 from E.coli is susceptible to CdiA-EC93 and to CdiA-CT from strain 536 / UPEC (PubMed:23882017)." /translation="MAMKKLLIASLLFSSATVYGAEGFVVKDIHFEGLQRVAVGAALLSMPVRTGDTVNDEDISNTIRALFATGNFEDVRVLRDGDTLLVQVKERPTIASITFSGNKSVKDDMLKQNLEASGVRVGESLDRTTIADIEKGLEDFYYSVGKYSASVKAVVTPLPRNRVDLKLVFQEGVSAEIQQINIVGNHAFTTDELISHFQLRDEVPWWNVVGDRKYQKQKLAGDLETLRSYYLDRGYARFNIDSTQVSLTPDKKGIYVTVNITEGDQYKLSGVEVSGNLAGHSAEIEQLTKIEPGELYNGTKVTKMEDDIKKLLGRYGYAYPRVQSMPEINDADKTVKLRVNVDAGNRFYVRKIRFEGNDTSKDAVLRREMRQMEGAWLGSDLVDQGKERLNRLGFFETVDTDTQRVPGSPDQVDVVYKVKERNTGSFNFGIGYGTESGVSFQAGVQQDNWLGTGYAVGINGTKNDYQTYAELSVTNPYFTVDGVSLGGRLFYNDFQADDADLSDYTNKSYGTDVTLGFPINEYNSLRAGLGYVHNSLSNMQPQVAMWRYLYSMGEHPSTSDQDNSFKTDDFTFNYGWTYNKLDRGYFPTDGSRVNLTGKVTIPGSDNEYYKVTLDTATYVPIDDDHKWVVLGRTRWGYGDGLGGKEMPFYENFYAGGSSTVRGFQSNTIGPKAVYFPHQASNYDPDYDYECATQDGAKDLCKSDDAVGGNAMAVASLEFITPTPFISDKYANSVRTSFFWDMGTVWDTNWDSSQYSGYPDYSDPSNIRMSAGIALQWMSPLGPLVFSYAQPFKKYDGDKAEQFQFNIGKTW" /label="bamA" primer_bind complement(90..106) /label="M13 rev" /note="common sequencing primer, one of multiple similar variants" rep_origin complement(173..761) /label="ori" /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(952..1812) /label="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*" promoter complement(1813..1917) /label="bla promoter" /note="ampicillin resistance gene promoter" misc_feature 4497..4556 /label="FreeGenes Barcode" /note="FreeGenes Barcode. Defined as I-SceI site flanked by 21 base pair random sequences." ORIGIN 1 CGCTGAGGTG TCAATCGTCG GAGCCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC 61 TAAACGGGTC TTGAGGGGTT TTTTGCATGG TCATAGCTGT TTCCTGAGAG CTTGGCAGGT 121 GATGACACAC ATTAACAAAT TTCGTGAGGA GTCTCCAGAA GAATGCCATT AATTTCCATA 181 GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC 241 CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG 301 TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC 361 TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG 421 GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC 481 TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA 541 TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG 601 GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA 661 AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG 721 TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGGCCTA CTATTAGCAA 781 CAACGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA 841 AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA 901 ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAAACT TGGTCTGACA GTTACCAATG 961 CTTAATCAGT GAGGCACCTA TCTCAGCGAT CTGTCTATTT CGTTCATCCA TAGTTGCCTG 1021 ACTCCCCGTC GTGTAGATAA CTACGATACG GGAGGGCTTA CCATCTGGCC CCAGTGCTGC 1081 AATGATACCG CGAGAACCAC GCTCACCGGC TCCAGATTTA TCAGCAATAA ACCAGCCAGC 1141 CGGAAGGGCC GAGCGCAGAA GTGGTCCTGC AACTTTATCC GCCTCCATCC AGTCTATTAA 1201 TTGTTGCCGG GAAGCTAGAG TAAGTAGTTC GCCAGTTAAT AGTTTGCGCA ACGTTGTTGC 1261 CATTGCTACA GGCATCGTGG TGTCACGCTC GTCGTTTGGT ATGGCTTCAT TCAGCTCCGG 1321 TTCCCAACGA TCAAGGCGAG TTACATGATC CCCCATGTTG TGCAAAAAAG CGGTTAGCTC 1381 CTTCGGTCCT CCGATCGTTG TCAGAAGTAA GTTGGCCGCA GTGTTATCAC TCATGGTTAT 1441 GGCAGCACTG CATAATTCTC TTACTGTCAT GCCATCCGTA AGATGCTTTT CTGTGACTGG 1501 TGAGTACTCA ACCAAGTCAT TCTGAGAATA GTGTATGCGG CGACCGAGTT GCTCTTGCCC 1561 GGCGTCAATA CGGGATAATA CCGCGCCACA TAGCAGAACT TTAAAAGTGC TCATCATTGG 1621 AAAACGTTCT TCGGGGCGAA AACTCTCAAG GATCTTACCG CTGTTGAGAT CCAGTTCGAT 1681 GTAACCCACT CGTGCACCCA ACTGATCTTC AGCATCTTTT ACTTTCACCA GCGTTTCTGG 1741 GTGAGCAAAA ACAGGAAGGC AAAATGCCGC AAAAAAGGGA ATAAGGGCGA CACGGAAATG 1801 TTGAATACTC ATACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT 1861 CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC 1921 CTGCACCAGT CAGTAAAACG ACGGCCAGTA GTCAAAAGCC TCCGACCGGA GGCTTTTGAC 1981 TTGGTTCAGG TGGAGTGGGA GAAAGCACTC TTAGGCCTCT GGGTCTCTAA TGGCAATGAA 2041 AAAACTACTA ATCGCATCAC TACTATTCTC ATCAGCAACC GTCTACGGAG CAGAAGGATT 2101 CGTCGTCAAA GACATCCACT TCGAAGGACT ACAGCGTGTC GCAGTCGGAG CAGCACTACT 2161 ATCAATGCCG GTCCGTACCG GAGACACCGT CAACGACGAA GACATCTCAA ACACCATCCG 2221 TGCACTATTC GCAACCGGAA ACTTCGAAGA CGTCCGTGTC CTACGTGACG GAGACACCCT 2281 ACTAGTCCAG GTCAAAGAAC GTCCGACCAT CGCATCAATC ACCTTCTCAG GAAACAAATC 2341 AGTCAAAGAC GACATGCTAA AACAGAACCT AGAAGCATCA GGAGTCCGTG TCGGAGAATC 2401 ACTAGACCGT ACCACCATCG CAGACATCGA AAAAGGACTA GAAGACTTCT ACTACTCAGT 2461 CGGAAAATAC TCAGCATCAG TCAAAGCAGT CGTCACCCCG CTACCGCGTA ACCGTGTCGA 2521 CCTAAAACTA GTCTTCCAGG AAGGAGTCTC AGCAGAAATC CAGCAGATCA ACATCGTCGG 2581 AAACCACGCA TTCACCACCG ACGAACTAAT CTCACACTTC CAGCTACGTG ACGAAGTCCC 2641 GTGGTGGAAC GTCGTCGGAG ACCGTAAATA CCAGAAACAG AAACTAGCAG GAGACCTAGA 2701 AACCCTACGT TCATACTACC TAGACCGTGG ATACGCACGT TTCAACATCG ACTCAACCCA 2761 GGTCTCACTA ACCCCGGACA AAAAAGGAAT CTACGTCACC GTCAACATCA CCGAAGGAGA 2821 CCAGTACAAA CTATCAGGAG TCGAAGTCTC AGGAAACCTA GCAGGACACT CAGCAGAAAT 2881 CGAACAGCTA ACCAAAATCG AACCGGGAGA ACTATACAAC GGAACCAAAG TCACCAAAAT 2941 GGAAGACGAC ATCAAAAAAC TACTAGGACG TTACGGATAC GCATACCCGC GTGTCCAGTC 3001 AATGCCGGAA ATCAACGACG CAGACAAAAC CGTCAAACTA CGTGTCAACG TCGACGCAGG 3061 AAACCGTTTC TACGTCCGTA AAATCCGTTT CGAAGGAAAC GACACCTCAA AAGACGCAGT 3121 CCTACGTCGT GAAATGCGTC AGATGGAAGG AGCATGGCTA GGATCAGACC TAGTCGACCA 3181 GGGAAAAGAA CGTCTAAACC GTCTAGGATT CTTCGAAACC GTCGACACCG ACACCCAGCG 3241 TGTCCCGGGA TCACCGGACC AGGTCGACGT CGTCTACAAA GTCAAAGAAC GTAACACCGG 3301 ATCATTCAAC TTCGGAATCG GATACGGAAC CGAATCAGGA GTCTCATTCC AGGCAGGAGT 3361 CCAGCAGGAC AACTGGCTAG GAACCGGATA CGCAGTCGGA ATCAACGGAA CCAAAAACGA 3421 CTACCAGACC TACGCAGAAC TATCAGTCAC CAACCCGTAC TTCACCGTCG ACGGAGTCTC 3481 ACTAGGAGGA CGTCTATTCT ACAACGACTT CCAGGCAGAC GACGCAGACC TATCAGACTA 3541 CACCAACAAA TCATACGGAA CCGACGTCAC CCTAGGATTC CCGATCAACG AATACAACTC 3601 ACTACGTGCA GGACTAGGAT ACGTCCACAA CTCACTATCA AACATGCAGC CGCAGGTCGC 3661 AATGTGGCGT TACCTATACT CAATGGGAGA ACACCCGTCA ACCTCAGACC AGGACAACTC 3721 ATTCAAAACC GACGACTTCA CCTTCAACTA CGGATGGACC TACAACAAAC TAGACCGTGG 3781 ATACTTCCCG ACCGACGGAT CACGTGTCAA CCTAACCGGA AAAGTCACCA TCCCGGGATC 3841 AGACAACGAA TACTACAAAG TCACCCTAGA CACCGCAACC TACGTCCCGA TCGACGACGA 3901 CCACAAATGG GTCGTCCTAG GACGTACCCG TTGGGGATAC GGAGACGGAC TAGGAGGAAA 3961 AGAAATGCCG TTCTACGAAA ACTTCTACGC AGGAGGATCA TCAACCGTCC GTGGATTCCA 4021 GTCAAACACC ATCGGACCGA AAGCAGTCTA CTTCCCGCAC CAGGCATCAA ACTACGACCC 4081 GGACTACGAC TACGAATGCG CAACCCAGGA CGGAGCAAAA GACCTATGCA AATCAGACGA 4141 CGCAGTCGGA GGAAACGCAA TGGCAGTCGC ATCACTAGAA TTCATCACCC CGACCCCGTT 4201 CATCTCAGAC AAATACGCAA ACTCAGTCCG TACCTCATTC TTCTGGGACA TGGGAACCGT 4261 CTGGGACACC AACTGGGACT CATCACAGTA CTCAGGATAC CCGGACTACT CAGACCCGTC 4321 AAACATCCGT ATGTCAGCAG GAATCGCACT ACAGTGGATG TCACCGCTAG GACCGCTAGT 4381 CTTCTCATAC GCACAGCCGT TCAAAAAATA CGACGGAGAC AAAGCAGAAC AGTTCCAGTT 4441 CAACATCGGA AAAACCTGGT GAAGAGCTTC GAGACCATTC TCACGACGCA AGATGGGTAC 4501 CCGCTAGTAA GTAGTTATAG GGATAACAGG GTAATTGCAA AAGCTATGTC TTTTTT //