LOCUS       Exported     3842 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..3842
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
                     /label="T7 terminator"
     terminator      1879..1926
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     3774..3790
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      3791..3822
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             100..3841
                     /label="proS"
                     /note="Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction: proline is first activated by ATP to form Pro-AMP and then transferred to the acceptor end of tRNA(Pro). As ProRS can inadvertently accommodate and process non-cognate amino acids such as alanine and cysteine, to avoid such errors it has two additional distinct editing activities against alanine. One activity is designated as 'pretransfer' editing and involves the tRNA(Pro)-independent hydrolysis of activated Ala-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Ala-tRNA(Pro). Misacylated Cys-tRNA(Pro) is not edited by ProRS, but instead may be edited in trans by YbaK."
                     /translation="MRTSQYLLSTLKETPADAEVISHQLMLRAGMIRKLASGLYTWLPTGVRVLKKVENIVREEMNNAGAIEVSMPVVQPADLWQESGRWEQYGPELLRFVDRGERPFVLGPTHEEVITDLIRNELSSYKQLPLNFYQIQTKFRDEVRPRFGVMRSREFLMKDAYSFHTSQESLQETYDAMYAAYSKIFSRMGLDFRAVQADTGSIGGSASHEFQVLAQSGEDDVVFSDTSDYAANIELAEAIAPKEPRAAATQEMTLVDTPNAKTIAELVEQFNLPIEKTVKTLLVKAVEGSSFPQVALLVRGDHELNEVKAEKLPQVASPLTFATEEEIRAVVKAGPGSLGPVNMPIPVVIDRTVAAMSDFAAGANIDGKHYFGINWDRDVATPEVADIRNVVAGDPSPDGQGRLLIKRGIEVGHIFQLGTKYSEALKASVQGEDGRNQILTMGCYGIGVTRVVAAAIEQNYDERGIVWPDAIAPFQVAILPMNMHKSFRVQELAEKLYSELRAQGIEVLLDDRKERPGVMFADMELIGIPHTIVLGDRNLDNDDIEYKYRRNGEKQLIKTGDIVEYLVKQIKG"
     primer_bind     complement(1931..1947)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(2014..2602)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(2793..3653)
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
     promoter        complement(3654..3758)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
     misc_feature    1782..1841
                     /label="FreeGenes Barcode"
                     /note="FreeGenes Barcode. Defined as I-SceI site flanked by 21 base pair random sequences."
ORIGIN
        1 AAACCGGAGC CATACAGTAC GGTCTCTAAT GCGTACCTCA CAGTACCTAC TATCAACCCT
       61 AAAAGAAACC CCGGCAGACG CAGAAGTCAT CTCACACCAG CTAATGCTAC GTGCAGGAAT
      121 GATCCGTAAA CTAGCATCAG GACTATACAC CTGGCTACCG ACCGGAGTCC GTGTCCTAAA
      181 AAAAGTCGAA AACATCGTCC GTGAAGAAAT GAACAACGCA GGAGCAATCG AAGTCTCAAT
      241 GCCGGTCGTC CAGCCGGCAG ACCTATGGCA GGAATCAGGA CGTTGGGAAC AGTACGGACC
      301 GGAACTACTA CGTTTCGTCG ACCGTGGAGA ACGTCCGTTC GTCCTAGGAC CGACCCACGA
      361 AGAAGTCATC ACCGACCTAA TCCGTAACGA ACTATCATCA TACAAACAGC TACCGCTAAA
      421 CTTCTACCAG ATCCAGACCA AATTCCGTGA CGAAGTCCGT CCGCGTTTCG GAGTCATGCG
      481 TTCACGTGAA TTCCTAATGA AAGACGCATA CTCATTCCAC ACCTCACAGG AATCACTACA
      541 GGAAACCTAC GACGCAATGT ACGCAGCATA CTCAAAAATC TTCTCACGTA TGGGACTAGA
      601 CTTCCGTGCA GTCCAGGCAG ACACCGGATC AATCGGAGGA TCAGCATCAC ACGAATTCCA
      661 GGTCCTAGCA CAGTCAGGAG AAGACGACGT CGTCTTCTCA GACACCTCAG ACTACGCAGC
      721 AAACATCGAA CTAGCAGAAG CAATCGCACC GAAAGAACCG CGTGCAGCAG CAACCCAGGA
      781 AATGACCCTA GTCGACACCC CGAACGCAAA AACCATCGCA GAACTAGTCG AACAGTTCAA
      841 CCTACCGATC GAAAAAACCG TCAAAACCCT ACTAGTCAAA GCAGTCGAAG GATCATCATT
      901 CCCGCAGGTC GCACTACTAG TCCGTGGAGA CCACGAACTA AACGAAGTCA AAGCAGAAAA
      961 ACTACCGCAG GTCGCATCAC CGCTAACCTT CGCAACCGAA GAAGAAATCC GTGCAGTCGT
     1021 CAAAGCAGGA CCGGGATCAC TAGGACCGGT CAACATGCCG ATCCCGGTCG TCATCGACCG
     1081 TACCGTCGCA GCAATGTCAG ACTTCGCAGC AGGAGCAAAC ATCGACGGAA AACACTACTT
     1141 CGGAATCAAC TGGGACCGTG ACGTCGCAAC CCCGGAAGTC GCAGACATCC GTAACGTCGT
     1201 CGCAGGAGAC CCGTCACCGG ACGGACAGGG ACGTCTACTA ATCAAACGTG GAATCGAAGT
     1261 CGGACACATC TTCCAGCTAG GAACCAAATA CTCAGAAGCA CTAAAAGCAT CAGTCCAGGG
     1321 AGAAGACGGA CGTAACCAGA TCCTAACCAT GGGATGCTAC GGAATCGGAG TCACCCGTGT
     1381 CGTCGCAGCA GCAATCGAAC AGAACTACGA CGAACGTGGA ATCGTCTGGC CGGACGCAAT
     1441 CGCACCGTTC CAGGTCGCAA TCCTACCGAT GAACATGCAC AAATCATTCC GTGTCCAGGA
     1501 ACTAGCAGAA AAACTATACT CAGAACTACG TGCACAGGGA ATCGAAGTCC TACTAGACGA
     1561 CCGTAAAGAA CGTCCGGGAG TCATGTTCGC AGACATGGAA CTAATCGGAA TCCCGCACAC
     1621 CATCGTCCTA GGAGACCGTA ACCTAGACAA CGACGACATC GAATACAAAT ACCGTCGTAA
     1681 CGGAGAAAAA CAGCTAATCA AAACCGGAGA CATCGTCGAA TACCTAGTCA AACAGATCAA
     1741 AGGATGAAGA GCTTCGAGAC CACCACAGGT CAAGATTCAC GAATAAAATC CCAAACTTGC
     1801 AGTAGGGATA ACAGGGTAAT GATAATGGTT ACCGCTGGTC CCGCTGAGGT GTCAATCGTC
     1861 GGAGCCGCTG AGCAATAACT AGCATAACCC CTTGGGGCCT CTAAACGGGT CTTGAGGGGT
     1921 TTTTTGCATG GTCATAGCTG TTTCCTGAGA GCTTGGCAGG TGATGACACA CATTAACAAA
     1981 TTTCGTGAGG AGTCTCCAGA AGAATGCCAT TAATTTCCAT AGGCTCCGCC CCCCTGACGA
     2041 GCATCACAAA AATCGACGCT CAAGTCAGAG GTGGCGAAAC CCGACAGGAC TATAAAGATA
     2101 CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT GCGCTCTCCT GTTCCGACCC TGCCGCTTAC
     2161 CGGATACCTG TCCGCCTTTC TCCCTTCGGG AAGCGTGGCG CTTTCTCATA GCTCACGCTG
     2221 TAGGTATCTC AGTTCGGTGT AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC ACGAACCCCC
     2281 CGTTCAGCCC GACCGCTGCG CCTTATCCGG TAACTATCGT CTTGAGTCCA ACCCGGTAAG
     2341 ACACGACTTA TCGCCACTGG CAGCAGCCAC TGGTAACAGG ATTAGCAGAG CGAGGTATGT
     2401 AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG GCCTAACTAC GGCTACACTA GAAGAACAGT
     2461 ATTTGGTATC TGCGCTCTGC TGAAGCCAGT TACCTTCGGA AAAAGAGTTG GTAGCTCTTG
     2521 ATCCGGCAAA CAAACCACCG CTGGTAGCGG TGGTTTTTTT GTTTGCAAGC AGCAGATTAC
     2581 GCGCAGAAAA AAAGGATCTC AAGAAGGCCT ACTATTAGCA ACAACGATCC TTTGATCTTT
     2641 TCTACGGGGT CTGACGCTCA GTGGAACGAA AACTCACGTT AAGGGATTTT GGTCATGAGA
     2701 TTATCAAAAA GGATCTTCAC CTAGATCCTT TTAAATTAAA AATGAAGTTT TAAATCAATC
     2761 TAAAGTATAT ATGAGTAAAC TTGGTCTGAC AGTTACCAAT GCTTAATCAG TGAGGCACCT
     2821 ATCTCAGCGA TCTGTCTATT TCGTTCATCC ATAGTTGCCT GACTCCCCGT CGTGTAGATA
     2881 ACTACGATAC GGGAGGGCTT ACCATCTGGC CCCAGTGCTG CAATGATACC GCGAGAACCA
     2941 CGCTCACCGG CTCCAGATTT ATCAGCAATA AACCAGCCAG CCGGAAGGGC CGAGCGCAGA
     3001 AGTGGTCCTG CAACTTTATC CGCCTCCATC CAGTCTATTA ATTGTTGCCG GGAAGCTAGA
     3061 GTAAGTAGTT CGCCAGTTAA TAGTTTGCGC AACGTTGTTG CCATTGCTAC AGGCATCGTG
     3121 GTGTCACGCT CGTCGTTTGG TATGGCTTCA TTCAGCTCCG GTTCCCAACG ATCAAGGCGA
     3181 GTTACATGAT CCCCCATGTT GTGCAAAAAA GCGGTTAGCT CCTTCGGTCC TCCGATCGTT
     3241 GTCAGAAGTA AGTTGGCCGC AGTGTTATCA CTCATGGTTA TGGCAGCACT GCATAATTCT
     3301 CTTACTGTCA TGCCATCCGT AAGATGCTTT TCTGTGACTG GTGAGTACTC AACCAAGTCA
     3361 TTCTGAGAAT AGTGTATGCG GCGACCGAGT TGCTCTTGCC CGGCGTCAAT ACGGGATAAT
     3421 ACCGCGCCAC ATAGCAGAAC TTTAAAAGTG CTCATCATTG GAAAACGTTC TTCGGGGCGA
     3481 AAACTCTCAA GGATCTTACC GCTGTTGAGA TCCAGTTCGA TGTAACCCAC TCGTGCACCC
     3541 AACTGATCTT CAGCATCTTT TACTTTCACC AGCGTTTCTG GGTGAGCAAA AACAGGAAGG
     3601 CAAAATGCCG CAAAAAAGGG AATAAGGGCG ACACGGAAAT GTTGAATACT CATACTCTTC
     3661 CTTTTTCAAT ATTATTGAAG CATTTATCAG GGTTATTGTC TCATGAGCGG ATACATATTT
     3721 GAATGTATTT AGAAAAATAA ACAAATAGGG GTTCCGCGCA CCTGCACCAG TCAGTAAAAC
     3781 GACGGCCAGT AGTCAAAAGC CTCCGACCGG AGGCTTTTGA CTTGGTTCAG GTGGAGTGGG
     3841 AG
//