LOCUS Exported 3506 bp DNA circular SYN 24-JUL-2020 DEFINITION synthetic linear DNA ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2001) AUTHORS Keoni Gandall TITLE Direct Submission JOURNAL Exported from Poly. FEATURES Location/Qualifiers terminator 1..3506 /label="T7 terminator" /note="transcription terminator for bacteriophage T7 RNA polymerase" terminator 38..85 /note="transcription terminator for bacteriophage T7 RNA polymerase" /label="T7 terminator" primer_bind 1933..1949 /label="M13 for" /note="common sequencing primer, one of multiple similar variants" terminator 1950..1981 /note="bidirectional E. coli tonB-P14 transcription terminator" /label="tonB terminator" CDS 2030..3412 /note="" /translation="MTQLTMKDKIGYGLGDTACGFVWQATMFLLAYFYTDVFGLSAGIMGTLFLVSRVLDAVTDPLMGLLVDRTRTRHGQFRPFLLWGAIPFGIVCVLTFYTPDFSAQGKIIYACVTYILLTLVYTFVNVPYCAMPGVITADPKERHALQSWRFFLAAAGSLAISGIALPLVSIIGKGDEQVGYFGAMCVLGLSGVVLLYVCFFTTKERYTFEVQPGSSVAKDLKLLLGNSQWRIMCAFKMMATCSNVVRGGATLYFVKYVMDHPELATQFLLYGSLATMFGSLCSSRLLGRFDRVTAFKWIIVAYSLISLLIFVTPAEHIALIFALNILFLFVFNTTTPLQWLMASDVVDYEESRSGRRLDGLVFSTYLFSLKIGLAIGGAVVGWILAYVNYSASSSVQPVEVLTTIKILFCVVPVVLYAGMFIMLSLYKLTDARVEAISRQLIKHRAAQGEAVPDAATAASH" /label="yagG" primer_bind complement(90..106) /label="M13 rev" /note="common sequencing primer, one of multiple similar variants" rep_origin complement(173..761) /label="ori" /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(952..1812) /label="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*" promoter complement(1813..1917) /label="bla promoter" /note="ampicillin resistance gene promoter" misc_feature 3447..3506 /note="FreeGenes Barcode. Defined as I-SceI site flanked by 21 base pair random sequences." /label="FreeGenes Barcode" ORIGIN 1 CGCTGAGGTG TCAATCGTCG GAGCCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC 61 TAAACGGGTC TTGAGGGGTT TTTTGCATGG TCATAGCTGT TTCCTGAGAG CTTGGCAGGT 121 GATGACACAC ATTAACAAAT TTCGTGAGGA GTCTCCAGAA GAATGCCATT AATTTCCATA 181 GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC 241 CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG 301 TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC 361 TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG 421 GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC 481 TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA 541 TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG 601 GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA 661 AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG 721 TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGGCCTA CTATTAGCAA 781 CAACGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA 841 AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA 901 ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAAACT TGGTCTGACA GTTACCAATG 961 CTTAATCAGT GAGGCACCTA TCTCAGCGAT CTGTCTATTT CGTTCATCCA TAGTTGCCTG 1021 ACTCCCCGTC GTGTAGATAA CTACGATACG GGAGGGCTTA CCATCTGGCC CCAGTGCTGC 1081 AATGATACCG CGAGAACCAC GCTCACCGGC TCCAGATTTA TCAGCAATAA ACCAGCCAGC 1141 CGGAAGGGCC GAGCGCAGAA GTGGTCCTGC AACTTTATCC GCCTCCATCC AGTCTATTAA 1201 TTGTTGCCGG GAAGCTAGAG TAAGTAGTTC GCCAGTTAAT AGTTTGCGCA ACGTTGTTGC 1261 CATTGCTACA GGCATCGTGG TGTCACGCTC GTCGTTTGGT ATGGCTTCAT TCAGCTCCGG 1321 TTCCCAACGA TCAAGGCGAG TTACATGATC CCCCATGTTG TGCAAAAAAG CGGTTAGCTC 1381 CTTCGGTCCT CCGATCGTTG TCAGAAGTAA GTTGGCCGCA GTGTTATCAC TCATGGTTAT 1441 GGCAGCACTG CATAATTCTC TTACTGTCAT GCCATCCGTA AGATGCTTTT CTGTGACTGG 1501 TGAGTACTCA ACCAAGTCAT TCTGAGAATA GTGTATGCGG CGACCGAGTT GCTCTTGCCC 1561 GGCGTCAATA CGGGATAATA CCGCGCCACA TAGCAGAACT TTAAAAGTGC TCATCATTGG 1621 AAAACGTTCT TCGGGGCGAA AACTCTCAAG GATCTTACCG CTGTTGAGAT CCAGTTCGAT 1681 GTAACCCACT CGTGCACCCA ACTGATCTTC AGCATCTTTT ACTTTCACCA GCGTTTCTGG 1741 GTGAGCAAAA ACAGGAAGGC AAAATGCCGC AAAAAAGGGA ATAAGGGCGA CACGGAAATG 1801 TTGAATACTC ATACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT 1861 CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC 1921 CTGCACCAGT CAGTAAAACG ACGGCCAGTA GTCAAAAGCC TCCGACCGGA GGCTTTTGAC 1981 TTGGTTCAGG TGGAGTGGGA GAAACCGGAG CCATACAGTA CGGTCTCTAA TGACCCAGCT 2041 AACCATGAAA GACAAAATCG GATACGGACT AGGAGACACC GCATGCGGAT TCGTCTGGCA 2101 GGCAACCATG TTCCTACTAG CATACTTCTA CACCGACGTC TTCGGACTAT CAGCAGGAAT 2161 CATGGGAACC CTATTCCTAG TCTCACGTGT CCTAGACGCA GTCACCGACC CGCTAATGGG 2221 ACTACTAGTC GACCGTACCC GTACCCGTCA CGGACAGTTC CGTCCGTTCC TACTATGGGG 2281 AGCAATCCCG TTCGGAATCG TCTGCGTCCT AACCTTCTAC ACCCCGGACT TCTCAGCACA 2341 GGGAAAAATC ATCTACGCAT GCGTCACCTA CATCCTACTA ACCCTAGTCT ACACCTTCGT 2401 CAACGTCCCG TACTGCGCAA TGCCGGGAGT CATCACCGCA GACCCGAAAG AACGTCACGC 2461 ACTACAGTCA TGGCGTTTCT TCCTAGCAGC AGCAGGATCA CTAGCAATCT CAGGAATCGC 2521 ACTACCGCTA GTCTCAATCA TCGGAAAAGG AGACGAACAG GTCGGATACT TCGGAGCAAT 2581 GTGCGTCCTA GGACTATCAG GAGTCGTCCT ACTATACGTC TGCTTCTTCA CCACCAAAGA 2641 ACGTTACACC TTCGAAGTCC AGCCGGGATC ATCAGTCGCA AAAGACCTAA AACTACTACT 2701 AGGAAACTCA CAGTGGCGTA TCATGTGCGC ATTCAAAATG ATGGCAACCT GCTCAAACGT 2761 CGTCCGTGGA GGAGCAACCC TATACTTCGT CAAATACGTC ATGGACCACC CGGAACTAGC 2821 AACCCAGTTC CTACTATACG GATCACTAGC AACCATGTTC GGATCACTAT GCTCATCACG 2881 TCTACTAGGA CGTTTCGACC GTGTCACCGC ATTCAAATGG ATCATCGTCG CATACTCACT 2941 AATCTCACTA CTAATCTTCG TCACCCCGGC AGAACACATC GCACTAATCT TCGCACTAAA 3001 CATCCTATTC CTATTCGTCT TCAACACCAC CACCCCGCTA CAGTGGCTAA TGGCATCAGA 3061 CGTCGTCGAC TACGAAGAAT CACGTTCAGG ACGTCGTCTA GACGGACTAG TCTTCTCAAC 3121 CTACCTATTC TCACTAAAAA TCGGACTAGC AATCGGAGGA GCAGTCGTCG GATGGATCCT 3181 AGCATACGTC AACTACTCAG CATCATCATC AGTCCAGCCG GTCGAAGTCC TAACCACCAT 3241 CAAAATCCTA TTCTGCGTCG TCCCGGTCGT CCTATACGCA GGAATGTTCA TCATGCTATC 3301 ACTATACAAA CTAACCGACG CACGTGTCGA AGCAATCTCA CGTCAGCTAA TCAAACACCG 3361 TGCAGCACAG GGAGAAGCAG TCCCGGACGC AGCAACCGCA GCATCACACT GAAGAGCTTC 3421 GAGACCAGTC CGACACAATG TGACACCATT ACGCGGCAAA TGGCGGATAG GGATAACAGG 3481 GTAATCAGGA CGTTTAGCCA ATCGAT //