LOCUS       Exported     3098 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..3098
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
                     /label="T7 terminator"
     terminator      1135..1182
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     3030..3046
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      3047..3078
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             100..3097
                     /label="hemB"
                     /note="Catalyzes an early step in the biosynthesis of tetrapyrroles. Binds two molecules of 5-aminolevulinate per subunit, each at a distinct site, and catalyzes their condensation to form porphobilinogen."
                     /translation="MTDLIQRPRRLRKSPALRAMFEETTLSLNDLVLPIFVEEEIDDYKAVEAMPGVMRIPEKHLAREIERIANAGIRSVMTFGISHHTDETGSDAWREDGLVARMSRICKQTVPEMIVMSDTCFCEYTSHGHCGVLCEHGVDNDATLENLGKQAVVAAAAGADFIAPSAAMDGQVQAIRQALDAAGFKDTAIMSYSTKFASSFYGPFREAAGSALKGDRKSYQMNPMNRREAIRESLLDEAQGADCLMVKPAGAYLDIVRELRERTELPIGAYQVSGEYAMIKFAALAGAIDEEKVVLESLGSIKRAGADLIFSYFALDLAEKKILR"
     primer_bind     complement(1187..1203)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(1270..1858)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(2049..2909)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(2910..3014)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
     misc_feature    1038..1097
                     /label="FreeGenes Barcode"
                     /note="FreeGenes Barcode. Defined as I-SceI site flanked by 21 base pair random sequences."
ORIGIN
        1 AAAGCACTCT TAGGCCTCTG GGTCTCTAAT GACCGACCTA ATCCAGCGTC CGCGTCGTCT
       61 ACGTAAATCA CCGGCACTAC GTGCAATGTT CGAAGAAACC ACCCTATCAC TAAACGACCT
      121 AGTCCTACCG ATCTTCGTCG AAGAAGAAAT CGACGACTAC AAAGCAGTCG AAGCAATGCC
      181 GGGAGTCATG CGTATCCCGG AAAAACACCT AGCACGTGAA ATCGAACGTA TCGCAAACGC
      241 AGGAATCCGT TCAGTCATGA CCTTCGGAAT CTCACACCAC ACCGACGAAA CCGGATCAGA
      301 CGCATGGCGT GAAGACGGAC TAGTCGCACG TATGTCACGT ATCTGCAAAC AGACCGTCCC
      361 GGAAATGATC GTCATGTCAG ACACCTGCTT CTGCGAATAC ACCTCACACG GACACTGCGG
      421 AGTCCTATGC GAACACGGAG TCGACAACGA CGCAACCCTA GAAAACCTAG GAAAACAGGC
      481 AGTCGTCGCA GCAGCAGCAG GAGCAGACTT CATCGCACCG TCAGCAGCAA TGGACGGACA
      541 GGTCCAGGCA ATCCGTCAGG CACTAGACGC AGCAGGATTC AAAGACACCG CAATCATGTC
      601 ATACTCAACC AAATTCGCAT CATCATTCTA CGGACCGTTC CGTGAAGCAG CAGGATCAGC
      661 ACTAAAAGGA GACCGTAAAT CATACCAGAT GAACCCGATG AACCGTCGTG AAGCAATCCG
      721 TGAATCACTA CTAGACGAAG CACAGGGAGC AGACTGCCTA ATGGTCAAAC CGGCAGGAGC
      781 ATACCTAGAC ATCGTCCGTG AACTACGTGA ACGTACCGAA CTACCGATCG GAGCATACCA
      841 GGTCTCAGGA GAATACGCAA TGATCAAATT CGCAGCACTA GCAGGAGCAA TCGACGAAGA
      901 AAAAGTCGTC CTAGAATCAC TAGGATCAAT CAAACGTGCA GGAGCAGACC TAATCTTCTC
      961 ATACTTCGCA CTAGACCTAG CAGAAAAAAA AATCCTACGT TGAAGAGCTT CGAGACCACA
     1021 ACGAGCAGAC CGAATAGTGA CTGATAGTTT GAGGCCGATA GGGATAACAG GGTAATCCAA
     1081 CCAGAGATTT CGTATTCCGC TGAGGTGTCA ATCGTCGGAG CCGCTGAGCA ATAACTAGCA
     1141 TAACCCCTTG GGGCCTCTAA ACGGGTCTTG AGGGGTTTTT TGCATGGTCA TAGCTGTTTC
     1201 CTGAGAGCTT GGCAGGTGAT GACACACATT AACAAATTTC GTGAGGAGTC TCCAGAAGAA
     1261 TGCCATTAAT TTCCATAGGC TCCGCCCCCC TGACGAGCAT CACAAAAATC GACGCTCAAG
     1321 TCAGAGGTGG CGAAACCCGA CAGGACTATA AAGATACCAG GCGTTTCCCC CTGGAAGCTC
     1381 CCTCGTGCGC TCTCCTGTTC CGACCCTGCC GCTTACCGGA TACCTGTCCG CCTTTCTCCC
     1441 TTCGGGAAGC GTGGCGCTTT CTCATAGCTC ACGCTGTAGG TATCTCAGTT CGGTGTAGGT
     1501 CGTTCGCTCC AAGCTGGGCT GTGTGCACGA ACCCCCCGTT CAGCCCGACC GCTGCGCCTT
     1561 ATCCGGTAAC TATCGTCTTG AGTCCAACCC GGTAAGACAC GACTTATCGC CACTGGCAGC
     1621 AGCCACTGGT AACAGGATTA GCAGAGCGAG GTATGTAGGC GGTGCTACAG AGTTCTTGAA
     1681 GTGGTGGCCT AACTACGGCT ACACTAGAAG AACAGTATTT GGTATCTGCG CTCTGCTGAA
     1741 GCCAGTTACC TTCGGAAAAA GAGTTGGTAG CTCTTGATCC GGCAAACAAA CCACCGCTGG
     1801 TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA GATTACGCGC AGAAAAAAAG GATCTCAAGA
     1861 AGGCCTACTA TTAGCAACAA CGATCCTTTG ATCTTTTCTA CGGGGTCTGA CGCTCAGTGG
     1921 AACGAAAACT CACGTTAAGG GATTTTGGTC ATGAGATTAT CAAAAAGGAT CTTCACCTAG
     1981 ATCCTTTTAA ATTAAAAATG AAGTTTTAAA TCAATCTAAA GTATATATGA GTAAACTTGG
     2041 TCTGACAGTT ACCAATGCTT AATCAGTGAG GCACCTATCT CAGCGATCTG TCTATTTCGT
     2101 TCATCCATAG TTGCCTGACT CCCCGTCGTG TAGATAACTA CGATACGGGA GGGCTTACCA
     2161 TCTGGCCCCA GTGCTGCAAT GATACCGCGA GAACCACGCT CACCGGCTCC AGATTTATCA
     2221 GCAATAAACC AGCCAGCCGG AAGGGCCGAG CGCAGAAGTG GTCCTGCAAC TTTATCCGCC
     2281 TCCATCCAGT CTATTAATTG TTGCCGGGAA GCTAGAGTAA GTAGTTCGCC AGTTAATAGT
     2341 TTGCGCAACG TTGTTGCCAT TGCTACAGGC ATCGTGGTGT CACGCTCGTC GTTTGGTATG
     2401 GCTTCATTCA GCTCCGGTTC CCAACGATCA AGGCGAGTTA CATGATCCCC CATGTTGTGC
     2461 AAAAAAGCGG TTAGCTCCTT CGGTCCTCCG ATCGTTGTCA GAAGTAAGTT GGCCGCAGTG
     2521 TTATCACTCA TGGTTATGGC AGCACTGCAT AATTCTCTTA CTGTCATGCC ATCCGTAAGA
     2581 TGCTTTTCTG TGACTGGTGA GTACTCAACC AAGTCATTCT GAGAATAGTG TATGCGGCGA
     2641 CCGAGTTGCT CTTGCCCGGC GTCAATACGG GATAATACCG CGCCACATAG CAGAACTTTA
     2701 AAAGTGCTCA TCATTGGAAA ACGTTCTTCG GGGCGAAAAC TCTCAAGGAT CTTACCGCTG
     2761 TTGAGATCCA GTTCGATGTA ACCCACTCGT GCACCCAACT GATCTTCAGC ATCTTTTACT
     2821 TTCACCAGCG TTTCTGGGTG AGCAAAAACA GGAAGGCAAA ATGCCGCAAA AAAGGGAATA
     2881 AGGGCGACAC GGAAATGTTG AATACTCATA CTCTTCCTTT TTCAATATTA TTGAAGCATT
     2941 TATCAGGGTT ATTGTCTCAT GAGCGGATAC ATATTTGAAT GTATTTAGAA AAATAAACAA
     3001 ATAGGGGTTC CGCGCACCTG CACCAGTCAG TAAAACGACG GCCAGTAGTC AAAAGCCTCC
     3061 GACCGGAGGC TTTTGACTTG GTTCAGGTGG AGTGGGAG
//