LOCUS       Exported     3971 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..3971
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      2008..2055
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     3903..3919
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      3920..3951
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             100..3970
                     /label="secD"
                     /note="Part of the Sec protein translocase complex. Interacts with the SecYEG preprotein conducting channel. SecDF uses the proton motive force (PMF) to complete protein translocation after the ATP-dependent function of SecA. The large periplasmic domain is thought to have a base and head domain joined by a hinge; movement of the hinge may be coupled to both proton transport and protein export, with the head domain capturing substrate, and a conformational change preventing backward movement and driving forward movement. Expression of V.alginolyticus SecD and SecF in E.coli confers Na(+)-dependent protein export, strongly suggesting SecDF functions via cation-coupled protein translocation."
                     /translation="MLNRYPLWKYVMLIVVIVIGLLYALPNLFGEDPAVQITGARGVAASEQTLIQVQKTLQEEKITAKSVALEEGAILARFDSTDTQLRAREALMGVMGDKYVVALNLAPATPRWLAAIHAEPMKLGLDLRGGVHFLMEVDMDTALGKLQEQNIDSLRSDLREKGIPYTTVRKENNYGLSITFRDAKARDEAIAYLSKRHPDLVISSQGSNQLRAVMSDARLSEAREYAVQQNINILRNRVNQLGVAEPVVQRQGADRIVVELPGIQDTARAKEILGATATLEFRLVNTNVDQAAAASGRVPGDSEVKQTREGQPVVLYKRVILTGDHITDSTSSQDEYNQPQVNISLDSAGGNIMSNFTKDNIGKPMATLFVEYKDSGKKDANGRAVLVKQEEVINIANIQSRLGNSFRITGINNPNEARQLSLLLRAGALIAPIQIVEERTIGPTLGMQNIEQGLEACLAGLLVSILFMIIFYKKFGLIATSALIANLILIVGIMSLLPGATLSMPGIAGIVLTLAVAVDANVLINERIKEELSNGRTVQQAIDEGYRGAFSSIFDANITTLIKVIILYAVGTGAIKGFAITTGIGVATSMFTAIVGTRAIVNLLYGGKRVKKLSI"
     primer_bind     complement(2060..2076)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(2143..2731)
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
                     /label="ori"
     CDS             complement(2922..3782)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(3783..3887)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
     misc_feature    1911..1970
                     /label="FreeGenes Barcode"
                     /note="FreeGenes Barcode. Defined as I-SceI site flanked by 21 base pair random sequences."
ORIGIN
        1 AAAGCACTCT TAGGCCTCTG GGTCTCTAAT GCTAAACCGT TACCCGCTAT GGAAATACGT
       61 CATGCTAATC GTCGTCATCG TCATCGGACT ACTATACGCA CTACCGAACC TATTCGGAGA
      121 AGACCCGGCA GTCCAGATCA CCGGAGCACG TGGAGTCGCA GCATCAGAAC AGACCCTAAT
      181 CCAGGTCCAG AAAACCCTAC AGGAAGAAAA AATCACCGCA AAATCAGTCG CACTAGAAGA
      241 AGGAGCAATC CTAGCACGTT TCGACTCAAC CGACACCCAG CTACGTGCAC GTGAAGCACT
      301 AATGGGAGTC ATGGGAGACA AATACGTCGT CGCACTAAAC CTAGCACCGG CAACCCCGCG
      361 TTGGCTAGCA GCAATCCACG CAGAACCGAT GAAACTAGGA CTAGACCTAC GTGGAGGAGT
      421 CCACTTCCTA ATGGAAGTCG ACATGGACAC CGCACTAGGA AAACTACAGG AACAGAACAT
      481 CGACTCACTA CGTTCAGACC TACGTGAAAA AGGAATCCCG TACACCACCG TCCGTAAAGA
      541 AAACAACTAC GGACTATCAA TCACCTTCCG TGACGCAAAA GCACGTGACG AAGCAATCGC
      601 ATACCTATCA AAACGTCACC CGGACCTAGT CATCTCATCA CAGGGATCAA ACCAGCTACG
      661 TGCAGTCATG TCAGACGCAC GTCTATCAGA AGCACGTGAA TACGCAGTCC AGCAGAACAT
      721 CAACATCCTA CGTAACCGTG TCAACCAGCT AGGAGTCGCA GAACCGGTCG TCCAGCGTCA
      781 GGGAGCAGAC CGTATCGTCG TCGAACTACC GGGAATCCAG GACACCGCAC GTGCAAAAGA
      841 AATCCTAGGA GCAACCGCAA CCCTAGAATT CCGTCTAGTC AACACCAACG TCGACCAGGC
      901 AGCAGCAGCA TCAGGACGTG TCCCGGGAGA CTCAGAAGTC AAACAGACCC GTGAAGGACA
      961 GCCGGTCGTC CTATACAAAC GTGTCATCCT AACCGGAGAC CACATCACCG ACTCAACCTC
     1021 ATCACAGGAC GAATACAACC AGCCGCAGGT CAACATCTCA CTAGACTCAG CAGGAGGAAA
     1081 CATCATGTCA AACTTCACCA AAGACAACAT CGGAAAACCG ATGGCAACCC TATTCGTCGA
     1141 ATACAAAGAC TCAGGAAAAA AAGACGCAAA CGGACGTGCA GTCCTAGTCA AACAGGAAGA
     1201 AGTCATCAAC ATCGCAAACA TCCAGTCACG TCTAGGAAAC TCATTCCGTA TCACCGGAAT
     1261 CAACAACCCG AACGAAGCAC GTCAGCTATC ACTACTACTA CGTGCAGGAG CACTAATCGC
     1321 ACCGATCCAG ATCGTCGAAG AACGTACCAT CGGACCGACC CTAGGAATGC AGAACATCGA
     1381 ACAGGGACTA GAAGCATGCC TAGCAGGACT ACTAGTCTCA ATCCTATTCA TGATCATCTT
     1441 CTACAAAAAA TTCGGACTAA TCGCAACCTC AGCACTAATC GCAAACCTAA TCCTAATCGT
     1501 CGGAATCATG TCACTACTAC CGGGAGCAAC CCTATCAATG CCGGGAATCG CAGGAATCGT
     1561 CCTAACCCTA GCAGTCGCAG TCGACGCAAA CGTCCTAATC AACGAACGTA TCAAAGAAGA
     1621 ACTATCAAAC GGACGTACCG TCCAGCAGGC AATCGACGAA GGATACCGTG GAGCATTCTC
     1681 ATCAATCTTC GACGCAAACA TCACCACCCT AATCAAAGTC ATCATCCTAT ACGCAGTCGG
     1741 AACCGGAGCA ATCAAAGGAT TCGCAATCAC CACCGGAATC GGAGTCGCAA CCTCAATGTT
     1801 CACCGCAATC GTCGGAACCC GTGCAATCGT CAACCTACTA TACGGAGGAA AACGTGTCAA
     1861 AAAACTATCA ATCTGAAGAG CTTCGAGACC ACATTAAATT TCGCCGTGGC TGCCTGTCAT
     1921 GATTTTGTGA ATAGGGATAA CAGGGTAATG ATTCTATGAG CGCGCGACAG CGCTGAGGTG
     1981 TCAATCGTCG GAGCCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC TAAACGGGTC
     2041 TTGAGGGGTT TTTTGCATGG TCATAGCTGT TTCCTGAGAG CTTGGCAGGT GATGACACAC
     2101 ATTAACAAAT TTCGTGAGGA GTCTCCAGAA GAATGCCATT AATTTCCATA GGCTCCGCCC
     2161 CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC CGACAGGACT
     2221 ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG TTCCGACCCT
     2281 GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC TTTCTCATAG
     2341 CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA
     2401 CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC TTGAGTCCAA
     2461 CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA TTAGCAGAGC
     2521 GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG GCTACACTAG
     2581 AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG
     2641 TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA
     2701 GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGGCCTA CTATTAGCAA CAACGATCCT
     2761 TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA AGGGATTTTG
     2821 GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA ATGAAGTTTT
     2881 AAATCAATCT AAAGTATATA TGAGTAAACT TGGTCTGACA GTTACCAATG CTTAATCAGT
     2941 GAGGCACCTA TCTCAGCGAT CTGTCTATTT CGTTCATCCA TAGTTGCCTG ACTCCCCGTC
     3001 GTGTAGATAA CTACGATACG GGAGGGCTTA CCATCTGGCC CCAGTGCTGC AATGATACCG
     3061 CGAGAACCAC GCTCACCGGC TCCAGATTTA TCAGCAATAA ACCAGCCAGC CGGAAGGGCC
     3121 GAGCGCAGAA GTGGTCCTGC AACTTTATCC GCCTCCATCC AGTCTATTAA TTGTTGCCGG
     3181 GAAGCTAGAG TAAGTAGTTC GCCAGTTAAT AGTTTGCGCA ACGTTGTTGC CATTGCTACA
     3241 GGCATCGTGG TGTCACGCTC GTCGTTTGGT ATGGCTTCAT TCAGCTCCGG TTCCCAACGA
     3301 TCAAGGCGAG TTACATGATC CCCCATGTTG TGCAAAAAAG CGGTTAGCTC CTTCGGTCCT
     3361 CCGATCGTTG TCAGAAGTAA GTTGGCCGCA GTGTTATCAC TCATGGTTAT GGCAGCACTG
     3421 CATAATTCTC TTACTGTCAT GCCATCCGTA AGATGCTTTT CTGTGACTGG TGAGTACTCA
     3481 ACCAAGTCAT TCTGAGAATA GTGTATGCGG CGACCGAGTT GCTCTTGCCC GGCGTCAATA
     3541 CGGGATAATA CCGCGCCACA TAGCAGAACT TTAAAAGTGC TCATCATTGG AAAACGTTCT
     3601 TCGGGGCGAA AACTCTCAAG GATCTTACCG CTGTTGAGAT CCAGTTCGAT GTAACCCACT
     3661 CGTGCACCCA ACTGATCTTC AGCATCTTTT ACTTTCACCA GCGTTTCTGG GTGAGCAAAA
     3721 ACAGGAAGGC AAAATGCCGC AAAAAAGGGA ATAAGGGCGA CACGGAAATG TTGAATACTC
     3781 ATACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT CATGAGCGGA
     3841 TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC CTGCACCAGT
     3901 CAGTAAAACG ACGGCCAGTA GTCAAAAGCC TCCGACCGGA GGCTTTTGAC TTGGTTCAGG
     3961 TGGAGTGGGA G
//