LOCUS       Exported     3227 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..3227
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      1264..1311
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     3159..3175
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      3176..3207
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
                     /label="tonB terminator"
     CDS             29..1132
                     /label="ribD"
                     /note="Converts 2,5-diamino-6-(ribosylamino)-4(3h)-pyrimidinone 5'-phosphate into 5-amino-6-(ribosylamino)-2,4(1h,3h)-pyrimidinedione 5'-phosphate."
                     /translation="MQDEYYMARALKLAQRGRFTTHPNPNVGCVIVKDGEIVGEGYHQRAGEPHAEVHALRMAGEKAKGATAYVTLEPCSHHGRTPPCCDALIAAGVARVVASMQDPNPQVAGRGLYRLQQAGIDVSHGLMMSEAEQLNKGFLKRMRTGFPYIQLKLGASLDGRTAMASGESQWITSPQARRDVQLLRAQSHAILTSSATVLADDPALTVRWSELDEQTQALYPQQNLRQPIRIVIDSQNRVTPVHRIVQQPGETWFARTQEDSREWPETVRTLLIPEHKGHLDLVVLMMQLGKQQINSIWVEAGPTLAGALLQAGLVDELIVYIAPKLLGSDARGLCTLPGLEKLADAPQFKFKEIRHVGPDVCLHLVGA"
     primer_bind     complement(1316..1332)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(1399..1987)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(2178..3038)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(3039..3143)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
     misc_feature    1167..1226
                     /label="FreeGenes Barcode"
                     /note="FreeGenes Barcode. Defined as I-SceI site flanked by 21 base pair random sequences."
ORIGIN
        1 AAACACGTGG CAAACATTCC GGTCTCTAAT GCAGGACGAA TACTACATGG CACGTGCACT
       61 AAAACTAGCA CAGCGTGGAC GTTTCACCAC CCACCCGAAC CCGAACGTCG GATGCGTCAT
      121 CGTCAAAGAC GGAGAAATCG TCGGAGAAGG ATACCACCAG CGTGCAGGAG AACCGCACGC
      181 AGAAGTCCAC GCACTACGTA TGGCAGGAGA AAAAGCAAAA GGAGCAACCG CATACGTCAC
      241 CCTAGAACCG TGCTCACACC ACGGACGTAC CCCGCCGTGC TGCGACGCAC TAATCGCAGC
      301 AGGAGTCGCA CGTGTCGTCG CATCAATGCA GGACCCGAAC CCGCAGGTCG CAGGACGTGG
      361 ACTATACCGT CTACAGCAGG CAGGAATCGA CGTCTCACAC GGACTAATGA TGTCAGAAGC
      421 AGAACAGCTA AACAAAGGAT TCCTAAAACG TATGCGTACC GGATTCCCGT ACATCCAGCT
      481 AAAACTAGGA GCATCACTAG ACGGACGTAC CGCAATGGCA TCAGGAGAAT CACAGTGGAT
      541 CACCTCACCG CAGGCACGTC GTGACGTCCA GCTACTACGT GCACAGTCAC ACGCAATCCT
      601 AACCTCATCA GCAACCGTCC TAGCAGACGA CCCGGCACTA ACCGTCCGTT GGTCAGAACT
      661 AGACGAACAG ACCCAGGCAC TATACCCGCA GCAGAACCTA CGTCAGCCGA TCCGTATCGT
      721 CATCGACTCA CAGAACCGTG TCACCCCGGT CCACCGTATC GTCCAGCAGC CGGGAGAAAC
      781 CTGGTTCGCA CGTACCCAGG AAGACTCACG TGAATGGCCG GAAACCGTCC GTACCCTACT
      841 AATCCCGGAA CACAAAGGAC ACCTAGACCT AGTCGTCCTA ATGATGCAGC TAGGAAAACA
      901 GCAGATCAAC TCAATCTGGG TCGAAGCAGG ACCGACCCTA GCAGGAGCAC TACTACAGGC
      961 AGGACTAGTC GACGAACTAA TCGTCTACAT CGCACCGAAA CTACTAGGAT CAGACGCACG
     1021 TGGACTATGC ACCCTACCGG GACTAGAAAA ACTAGCAGAC GCACCGCAGT TCAAATTCAA
     1081 AGAAATCCGT CACGTCGGAC CGGACGTCTG CCTACACCTA GTCGGAGCAT GAAGAGCTTC
     1141 GAGACCAGGA CACCAGACCA ATGAAGAAGA GCTCGATCGT TTGCTCGTAG GGATAACAGG
     1201 GTAATGGTAC TTCCCGTTAA TCAGACCGCT GAGGTGTCAA TCGTCGGAGC CGCTGAGCAA
     1261 TAACTAGCAT AACCCCTTGG GGCCTCTAAA CGGGTCTTGA GGGGTTTTTT GCATGGTCAT
     1321 AGCTGTTTCC TGAGAGCTTG GCAGGTGATG ACACACATTA ACAAATTTCG TGAGGAGTCT
     1381 CCAGAAGAAT GCCATTAATT TCCATAGGCT CCGCCCCCCT GACGAGCATC ACAAAAATCG
     1441 ACGCTCAAGT CAGAGGTGGC GAAACCCGAC AGGACTATAA AGATACCAGG CGTTTCCCCC
     1501 TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC GACCCTGCCG CTTACCGGAT ACCTGTCCGC
     1561 CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC TCATAGCTCA CGCTGTAGGT ATCTCAGTTC
     1621 GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG TGTGCACGAA CCCCCCGTTC AGCCCGACCG
     1681 CTGCGCCTTA TCCGGTAACT ATCGTCTTGA GTCCAACCCG GTAAGACACG ACTTATCGCC
     1741 ACTGGCAGCA GCCACTGGTA ACAGGATTAG CAGAGCGAGG TATGTAGGCG GTGCTACAGA
     1801 GTTCTTGAAG TGGTGGCCTA ACTACGGCTA CACTAGAAGA ACAGTATTTG GTATCTGCGC
     1861 TCTGCTGAAG CCAGTTACCT TCGGAAAAAG AGTTGGTAGC TCTTGATCCG GCAAACAAAC
     1921 CACCGCTGGT AGCGGTGGTT TTTTTGTTTG CAAGCAGCAG ATTACGCGCA GAAAAAAAGG
     1981 ATCTCAAGAA GGCCTACTAT TAGCAACAAC GATCCTTTGA TCTTTTCTAC GGGGTCTGAC
     2041 GCTCAGTGGA ACGAAAACTC ACGTTAAGGG ATTTTGGTCA TGAGATTATC AAAAAGGATC
     2101 TTCACCTAGA TCCTTTTAAA TTAAAAATGA AGTTTTAAAT CAATCTAAAG TATATATGAG
     2161 TAAACTTGGT CTGACAGTTA CCAATGCTTA ATCAGTGAGG CACCTATCTC AGCGATCTGT
     2221 CTATTTCGTT CATCCATAGT TGCCTGACTC CCCGTCGTGT AGATAACTAC GATACGGGAG
     2281 GGCTTACCAT CTGGCCCCAG TGCTGCAATG ATACCGCGAG AACCACGCTC ACCGGCTCCA
     2341 GATTTATCAG CAATAAACCA GCCAGCCGGA AGGGCCGAGC GCAGAAGTGG TCCTGCAACT
     2401 TTATCCGCCT CCATCCAGTC TATTAATTGT TGCCGGGAAG CTAGAGTAAG TAGTTCGCCA
     2461 GTTAATAGTT TGCGCAACGT TGTTGCCATT GCTACAGGCA TCGTGGTGTC ACGCTCGTCG
     2521 TTTGGTATGG CTTCATTCAG CTCCGGTTCC CAACGATCAA GGCGAGTTAC ATGATCCCCC
     2581 ATGTTGTGCA AAAAAGCGGT TAGCTCCTTC GGTCCTCCGA TCGTTGTCAG AAGTAAGTTG
     2641 GCCGCAGTGT TATCACTCAT GGTTATGGCA GCACTGCATA ATTCTCTTAC TGTCATGCCA
     2701 TCCGTAAGAT GCTTTTCTGT GACTGGTGAG TACTCAACCA AGTCATTCTG AGAATAGTGT
     2761 ATGCGGCGAC CGAGTTGCTC TTGCCCGGCG TCAATACGGG ATAATACCGC GCCACATAGC
     2821 AGAACTTTAA AAGTGCTCAT CATTGGAAAA CGTTCTTCGG GGCGAAAACT CTCAAGGATC
     2881 TTACCGCTGT TGAGATCCAG TTCGATGTAA CCCACTCGTG CACCCAACTG ATCTTCAGCA
     2941 TCTTTTACTT TCACCAGCGT TTCTGGGTGA GCAAAAACAG GAAGGCAAAA TGCCGCAAAA
     3001 AAGGGAATAA GGGCGACACG GAAATGTTGA ATACTCATAC TCTTCCTTTT TCAATATTAT
     3061 TGAAGCATTT ATCAGGGTTA TTGTCTCATG AGCGGATACA TATTTGAATG TATTTAGAAA
     3121 AATAAACAAA TAGGGGTTCC GCGCACCTGC ACCAGTCAGT AAAACGACGG CCAGTAGTCA
     3181 AAAGCCTCCG ACCGGAGGCT TTTGACTTGG TTCAGGTGGA GTGGGAG
//