LOCUS       Exported     3986 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..3986
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      38..85
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     1933..1949
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      1950..1981
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             2030..3892
                     /translation="MSFDIAKYPTLALVDSTQELRLLPKESLPKLCDELRRYLLDSVSRSSGHFASGLGTVELTVALHYVYNTPFDQLIWDVGHQAYPHKILTGRRDKIGTIRQKGGLHPFPWRGESEYDVLSVGHSSTSISAGIGIAVAAEKEGKNRRTVCVIGDGAITAGMAFEAMNHAGDIRPDMLVILNDNEMSISENVGALNNHLAQLLSGKLYSSLREGGKKVFSGVPPIKELLKRTEEHIKGMVVPGTLFEELGFNYIGPVDGHDVLGLITTLKNMRDLKGPQFLHIMTKKGRGYEPAEKDPITFHAVPKFDPSSGCLPKSSGGLPSYSKIFGDWLCETAAKDNKLMAITPAMREGSGMVEFSRKFPDRYFDVAIAEQHAVTFAAGLAIGGYKPIVAIYSTFLQRAYDQVLHDVAIQKLPVLFAIDRAGIVGADGQTHQGAFDLSYLRCIPEMVIMTPSDENECRQMLYTGYHYNDGPSAVRYPRGNAVGVELTPLEKLPIGKGIVKRRGEKLAILNFGTLMPEAAKVAESLNATLVDMRFVKPLDEALILEMAASHEALVTVEENAIMGGAGSGVNEVLMAHRKPVPVLNIGLPDFFIPQGTQEEMRAELGLDAAGMEAKIKAWLA"
                     /label="dxs"
                     /note="Catalyzes the acyloin condensation reaction between C atoms 2 and 3 of pyruvate and glyceraldehyde 3-phosphate to yield 1-deoxy-D-xylulose-5-phosphate (DXP)."
     primer_bind     complement(90..106)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(173..761)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(952..1812)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(1813..1917)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
     misc_feature    3927..3986
                     /label="FreeGenes Barcode"
                     /note="FreeGenes Barcode. Defined as I-SceI site flanked by 21 base pair random sequences."
ORIGIN
        1 CGCTGAGGTG TCAATCGTCG GAGCCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC
       61 TAAACGGGTC TTGAGGGGTT TTTTGCATGG TCATAGCTGT TTCCTGAGAG CTTGGCAGGT
      121 GATGACACAC ATTAACAAAT TTCGTGAGGA GTCTCCAGAA GAATGCCATT AATTTCCATA
      181 GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC
      241 CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG
      301 TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC
      361 TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG
      421 GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC
      481 TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA
      541 TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG
      601 GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA
      661 AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG
      721 TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGGCCTA CTATTAGCAA
      781 CAACGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA
      841 AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA
      901 ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAAACT TGGTCTGACA GTTACCAATG
      961 CTTAATCAGT GAGGCACCTA TCTCAGCGAT CTGTCTATTT CGTTCATCCA TAGTTGCCTG
     1021 ACTCCCCGTC GTGTAGATAA CTACGATACG GGAGGGCTTA CCATCTGGCC CCAGTGCTGC
     1081 AATGATACCG CGAGAACCAC GCTCACCGGC TCCAGATTTA TCAGCAATAA ACCAGCCAGC
     1141 CGGAAGGGCC GAGCGCAGAA GTGGTCCTGC AACTTTATCC GCCTCCATCC AGTCTATTAA
     1201 TTGTTGCCGG GAAGCTAGAG TAAGTAGTTC GCCAGTTAAT AGTTTGCGCA ACGTTGTTGC
     1261 CATTGCTACA GGCATCGTGG TGTCACGCTC GTCGTTTGGT ATGGCTTCAT TCAGCTCCGG
     1321 TTCCCAACGA TCAAGGCGAG TTACATGATC CCCCATGTTG TGCAAAAAAG CGGTTAGCTC
     1381 CTTCGGTCCT CCGATCGTTG TCAGAAGTAA GTTGGCCGCA GTGTTATCAC TCATGGTTAT
     1441 GGCAGCACTG CATAATTCTC TTACTGTCAT GCCATCCGTA AGATGCTTTT CTGTGACTGG
     1501 TGAGTACTCA ACCAAGTCAT TCTGAGAATA GTGTATGCGG CGACCGAGTT GCTCTTGCCC
     1561 GGCGTCAATA CGGGATAATA CCGCGCCACA TAGCAGAACT TTAAAAGTGC TCATCATTGG
     1621 AAAACGTTCT TCGGGGCGAA AACTCTCAAG GATCTTACCG CTGTTGAGAT CCAGTTCGAT
     1681 GTAACCCACT CGTGCACCCA ACTGATCTTC AGCATCTTTT ACTTTCACCA GCGTTTCTGG
     1741 GTGAGCAAAA ACAGGAAGGC AAAATGCCGC AAAAAAGGGA ATAAGGGCGA CACGGAAATG
     1801 TTGAATACTC ATACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT
     1861 CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC
     1921 CTGCACCAGT CAGTAAAACG ACGGCCAGTA GTCAAAAGCC TCCGACCGGA GGCTTTTGAC
     1981 TTGGTTCAGG TGGAGTGGGA GAAACCGGAG CCATACAGTA CGGTCTCTAA TGTCATTCGA
     2041 CATCGCAAAA TACCCGACCC TAGCACTAGT CGACTCAACC CAGGAACTAC GTCTACTACC
     2101 GAAAGAATCA CTACCGAAAC TATGCGACGA ACTACGTCGT TACCTACTAG ACTCAGTCTC
     2161 ACGTTCATCA GGACACTTCG CATCAGGACT AGGAACCGTC GAACTAACCG TCGCACTACA
     2221 CTACGTCTAC AACACCCCGT TCGACCAGCT AATCTGGGAC GTCGGACACC AGGCATACCC
     2281 GCACAAAATC CTAACCGGAC GTCGTGACAA AATCGGAACC ATCCGTCAGA AAGGAGGACT
     2341 ACACCCGTTC CCGTGGCGTG GAGAATCAGA ATACGACGTC CTATCAGTCG GACACTCATC
     2401 AACCTCAATC TCAGCAGGAA TCGGAATCGC AGTCGCAGCA GAAAAAGAAG GAAAAAACCG
     2461 TCGTACCGTC TGCGTCATCG GAGACGGAGC AATCACCGCA GGAATGGCAT TCGAAGCAAT
     2521 GAACCACGCA GGAGACATCC GTCCGGACAT GCTAGTCATC CTAAACGACA ACGAAATGTC
     2581 AATCTCAGAA AACGTCGGAG CACTAAACAA CCACCTAGCA CAGCTACTAT CAGGAAAACT
     2641 ATACTCATCA CTACGTGAAG GAGGAAAAAA AGTCTTCTCA GGAGTCCCGC CGATCAAAGA
     2701 ACTACTAAAA CGTACCGAAG AACACATCAA AGGAATGGTC GTCCCGGGAA CCCTATTCGA
     2761 AGAACTAGGA TTCAACTACA TCGGACCGGT CGACGGACAC GACGTCCTAG GACTAATCAC
     2821 CACCCTAAAA AACATGCGTG ACCTAAAAGG ACCGCAGTTC CTACACATCA TGACCAAAAA
     2881 AGGACGTGGA TACGAACCGG CAGAAAAAGA CCCGATCACC TTCCACGCAG TCCCGAAATT
     2941 CGACCCGTCA TCAGGATGCC TACCGAAATC ATCAGGAGGA CTACCGTCAT ACTCAAAAAT
     3001 CTTCGGAGAC TGGCTATGCG AAACCGCAGC AAAAGACAAC AAACTAATGG CAATCACCCC
     3061 GGCAATGCGT GAAGGATCAG GAATGGTCGA ATTCTCACGT AAATTCCCGG ACCGTTACTT
     3121 CGACGTCGCA ATCGCAGAAC AGCACGCAGT CACCTTCGCA GCAGGACTAG CAATCGGAGG
     3181 ATACAAACCG ATCGTCGCAA TCTACTCAAC CTTCCTACAG CGTGCATACG ACCAGGTCCT
     3241 ACACGACGTC GCAATCCAGA AACTACCGGT CCTATTCGCA ATCGACCGTG CAGGAATCGT
     3301 CGGAGCAGAC GGACAGACCC ACCAGGGAGC ATTCGACCTA TCATACCTAC GTTGCATCCC
     3361 GGAAATGGTC ATCATGACCC CGTCAGACGA AAACGAATGC CGTCAGATGC TATACACCGG
     3421 ATACCACTAC AACGACGGAC CGTCAGCAGT CCGTTACCCG CGTGGAAACG CAGTCGGAGT
     3481 CGAACTAACC CCGCTAGAAA AACTACCGAT CGGAAAAGGA ATCGTCAAAC GTCGTGGAGA
     3541 AAAACTAGCA ATCCTAAACT TCGGAACCCT AATGCCGGAA GCAGCAAAAG TCGCAGAATC
     3601 ACTAAACGCA ACCCTAGTCG ACATGCGTTT CGTCAAACCG CTAGACGAAG CACTAATCCT
     3661 AGAAATGGCA GCATCACACG AAGCACTAGT CACCGTCGAA GAAAACGCAA TCATGGGAGG
     3721 AGCAGGATCA GGAGTCAACG AAGTCCTAAT GGCACACCGT AAACCGGTCC CGGTCCTAAA
     3781 CATCGGACTA CCGGACTTCT TCATCCCGCA GGGAACCCAG GAAGAAATGC GTGCAGAACT
     3841 AGGACTAGAC GCAGCAGGAA TGGAAGCAAA AATCAAAGCA TGGCTAGCAT GAAGAGCTTC
     3901 GAGACCAGTG TTTTCCATTT TCCGCGGAAA GAATCACACA GCCACGATAG GGATAACAGG
     3961 GTAATGTAGC AAGCGTTCCG CCAATT
//