LOCUS Exported 3986 bp DNA circular SYN 24-JUL-2020 DEFINITION synthetic linear DNA ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2001) AUTHORS Keoni Gandall TITLE Direct Submission JOURNAL Exported from Poly. FEATURES Location/Qualifiers terminator 1..3986 /label="T7 terminator" /note="transcription terminator for bacteriophage T7 RNA polymerase" terminator 38..85 /label="T7 terminator" /note="transcription terminator for bacteriophage T7 RNA polymerase" primer_bind 1933..1949 /label="M13 for" /note="common sequencing primer, one of multiple similar variants" terminator 1950..1981 /label="tonB terminator" /note="bidirectional E. coli tonB-P14 transcription terminator" CDS 2030..3892 /translation="MSFDIAKYPTLALVDSTQELRLLPKESLPKLCDELRRYLLDSVSRSSGHFASGLGTVELTVALHYVYNTPFDQLIWDVGHQAYPHKILTGRRDKIGTIRQKGGLHPFPWRGESEYDVLSVGHSSTSISAGIGIAVAAEKEGKNRRTVCVIGDGAITAGMAFEAMNHAGDIRPDMLVILNDNEMSISENVGALNNHLAQLLSGKLYSSLREGGKKVFSGVPPIKELLKRTEEHIKGMVVPGTLFEELGFNYIGPVDGHDVLGLITTLKNMRDLKGPQFLHIMTKKGRGYEPAEKDPITFHAVPKFDPSSGCLPKSSGGLPSYSKIFGDWLCETAAKDNKLMAITPAMREGSGMVEFSRKFPDRYFDVAIAEQHAVTFAAGLAIGGYKPIVAIYSTFLQRAYDQVLHDVAIQKLPVLFAIDRAGIVGADGQTHQGAFDLSYLRCIPEMVIMTPSDENECRQMLYTGYHYNDGPSAVRYPRGNAVGVELTPLEKLPIGKGIVKRRGEKLAILNFGTLMPEAAKVAESLNATLVDMRFVKPLDEALILEMAASHEALVTVEENAIMGGAGSGVNEVLMAHRKPVPVLNIGLPDFFIPQGTQEEMRAELGLDAAGMEAKIKAWLA" /label="dxs" /note="Catalyzes the acyloin condensation reaction between C atoms 2 and 3 of pyruvate and glyceraldehyde 3-phosphate to yield 1-deoxy-D-xylulose-5-phosphate (DXP)." primer_bind complement(90..106) /label="M13 rev" /note="common sequencing primer, one of multiple similar variants" rep_origin complement(173..761) /label="ori" /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(952..1812) /label="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*" promoter complement(1813..1917) /label="bla promoter" /note="ampicillin resistance gene promoter" misc_feature 3927..3986 /label="FreeGenes Barcode" /note="FreeGenes Barcode. Defined as I-SceI site flanked by 21 base pair random sequences." ORIGIN 1 CGCTGAGGTG TCAATCGTCG GAGCCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC 61 TAAACGGGTC TTGAGGGGTT TTTTGCATGG TCATAGCTGT TTCCTGAGAG CTTGGCAGGT 121 GATGACACAC ATTAACAAAT TTCGTGAGGA GTCTCCAGAA GAATGCCATT AATTTCCATA 181 GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC 241 CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG 301 TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC 361 TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG 421 GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC 481 TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA 541 TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG 601 GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA 661 AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG 721 TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGGCCTA CTATTAGCAA 781 CAACGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA 841 AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA 901 ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAAACT TGGTCTGACA GTTACCAATG 961 CTTAATCAGT GAGGCACCTA TCTCAGCGAT CTGTCTATTT CGTTCATCCA TAGTTGCCTG 1021 ACTCCCCGTC GTGTAGATAA CTACGATACG GGAGGGCTTA CCATCTGGCC CCAGTGCTGC 1081 AATGATACCG CGAGAACCAC GCTCACCGGC TCCAGATTTA TCAGCAATAA ACCAGCCAGC 1141 CGGAAGGGCC GAGCGCAGAA GTGGTCCTGC AACTTTATCC GCCTCCATCC AGTCTATTAA 1201 TTGTTGCCGG GAAGCTAGAG TAAGTAGTTC GCCAGTTAAT AGTTTGCGCA ACGTTGTTGC 1261 CATTGCTACA GGCATCGTGG TGTCACGCTC GTCGTTTGGT ATGGCTTCAT TCAGCTCCGG 1321 TTCCCAACGA TCAAGGCGAG TTACATGATC CCCCATGTTG TGCAAAAAAG CGGTTAGCTC 1381 CTTCGGTCCT CCGATCGTTG TCAGAAGTAA GTTGGCCGCA GTGTTATCAC TCATGGTTAT 1441 GGCAGCACTG CATAATTCTC TTACTGTCAT GCCATCCGTA AGATGCTTTT CTGTGACTGG 1501 TGAGTACTCA ACCAAGTCAT TCTGAGAATA GTGTATGCGG CGACCGAGTT GCTCTTGCCC 1561 GGCGTCAATA CGGGATAATA CCGCGCCACA TAGCAGAACT TTAAAAGTGC TCATCATTGG 1621 AAAACGTTCT TCGGGGCGAA AACTCTCAAG GATCTTACCG CTGTTGAGAT CCAGTTCGAT 1681 GTAACCCACT CGTGCACCCA ACTGATCTTC AGCATCTTTT ACTTTCACCA GCGTTTCTGG 1741 GTGAGCAAAA ACAGGAAGGC AAAATGCCGC AAAAAAGGGA ATAAGGGCGA CACGGAAATG 1801 TTGAATACTC ATACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT 1861 CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC 1921 CTGCACCAGT CAGTAAAACG ACGGCCAGTA GTCAAAAGCC TCCGACCGGA GGCTTTTGAC 1981 TTGGTTCAGG TGGAGTGGGA GAAACCGGAG CCATACAGTA CGGTCTCTAA TGTCATTCGA 2041 CATCGCAAAA TACCCGACCC TAGCACTAGT CGACTCAACC CAGGAACTAC GTCTACTACC 2101 GAAAGAATCA CTACCGAAAC TATGCGACGA ACTACGTCGT TACCTACTAG ACTCAGTCTC 2161 ACGTTCATCA GGACACTTCG CATCAGGACT AGGAACCGTC GAACTAACCG TCGCACTACA 2221 CTACGTCTAC AACACCCCGT TCGACCAGCT AATCTGGGAC GTCGGACACC AGGCATACCC 2281 GCACAAAATC CTAACCGGAC GTCGTGACAA AATCGGAACC ATCCGTCAGA AAGGAGGACT 2341 ACACCCGTTC CCGTGGCGTG GAGAATCAGA ATACGACGTC CTATCAGTCG GACACTCATC 2401 AACCTCAATC TCAGCAGGAA TCGGAATCGC AGTCGCAGCA GAAAAAGAAG GAAAAAACCG 2461 TCGTACCGTC TGCGTCATCG GAGACGGAGC AATCACCGCA GGAATGGCAT TCGAAGCAAT 2521 GAACCACGCA GGAGACATCC GTCCGGACAT GCTAGTCATC CTAAACGACA ACGAAATGTC 2581 AATCTCAGAA AACGTCGGAG CACTAAACAA CCACCTAGCA CAGCTACTAT CAGGAAAACT 2641 ATACTCATCA CTACGTGAAG GAGGAAAAAA AGTCTTCTCA GGAGTCCCGC CGATCAAAGA 2701 ACTACTAAAA CGTACCGAAG AACACATCAA AGGAATGGTC GTCCCGGGAA CCCTATTCGA 2761 AGAACTAGGA TTCAACTACA TCGGACCGGT CGACGGACAC GACGTCCTAG GACTAATCAC 2821 CACCCTAAAA AACATGCGTG ACCTAAAAGG ACCGCAGTTC CTACACATCA TGACCAAAAA 2881 AGGACGTGGA TACGAACCGG CAGAAAAAGA CCCGATCACC TTCCACGCAG TCCCGAAATT 2941 CGACCCGTCA TCAGGATGCC TACCGAAATC ATCAGGAGGA CTACCGTCAT ACTCAAAAAT 3001 CTTCGGAGAC TGGCTATGCG AAACCGCAGC AAAAGACAAC AAACTAATGG CAATCACCCC 3061 GGCAATGCGT GAAGGATCAG GAATGGTCGA ATTCTCACGT AAATTCCCGG ACCGTTACTT 3121 CGACGTCGCA ATCGCAGAAC AGCACGCAGT CACCTTCGCA GCAGGACTAG CAATCGGAGG 3181 ATACAAACCG ATCGTCGCAA TCTACTCAAC CTTCCTACAG CGTGCATACG ACCAGGTCCT 3241 ACACGACGTC GCAATCCAGA AACTACCGGT CCTATTCGCA ATCGACCGTG CAGGAATCGT 3301 CGGAGCAGAC GGACAGACCC ACCAGGGAGC ATTCGACCTA TCATACCTAC GTTGCATCCC 3361 GGAAATGGTC ATCATGACCC CGTCAGACGA AAACGAATGC CGTCAGATGC TATACACCGG 3421 ATACCACTAC AACGACGGAC CGTCAGCAGT CCGTTACCCG CGTGGAAACG CAGTCGGAGT 3481 CGAACTAACC CCGCTAGAAA AACTACCGAT CGGAAAAGGA ATCGTCAAAC GTCGTGGAGA 3541 AAAACTAGCA ATCCTAAACT TCGGAACCCT AATGCCGGAA GCAGCAAAAG TCGCAGAATC 3601 ACTAAACGCA ACCCTAGTCG ACATGCGTTT CGTCAAACCG CTAGACGAAG CACTAATCCT 3661 AGAAATGGCA GCATCACACG AAGCACTAGT CACCGTCGAA GAAAACGCAA TCATGGGAGG 3721 AGCAGGATCA GGAGTCAACG AAGTCCTAAT GGCACACCGT AAACCGGTCC CGGTCCTAAA 3781 CATCGGACTA CCGGACTTCT TCATCCCGCA GGGAACCCAG GAAGAAATGC GTGCAGAACT 3841 AGGACTAGAC GCAGCAGGAA TGGAAGCAAA AATCAAAGCA TGGCTAGCAT GAAGAGCTTC 3901 GAGACCAGTG TTTTCCATTT TCCGCGGAAA GAATCACACA GCCACGATAG GGATAACAGG 3961 GTAATGTAGC AAGCGTTCCG CCAATT //