LOCUS Exported 4055 bp DNA circular SYN 24-JUL-2020 DEFINITION synthetic linear DNA ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2001) AUTHORS Keoni Gandall TITLE Direct Submission JOURNAL Exported from Poly. FEATURES Location/Qualifiers terminator 1..4055 /note="transcription terminator for bacteriophage T7 RNA polymerase" /label="T7 terminator" terminator 38..85 /label="T7 terminator" /note="transcription terminator for bacteriophage T7 RNA polymerase" primer_bind 1933..1949 /label="M13 for" /note="common sequencing primer, one of multiple similar variants" terminator 1950..1981 /note="bidirectional E. coli tonB-P14 transcription terminator" /label="tonB terminator" CDS 100..4054 /label="dnaX" /note="interacts with the delta and delta' subunits to transfer the beta subunit on the DNA (PubMed:9927437). Interacts with ATP, drives ATP-induced conformational changes in the gamma complex that opens the beta sliding clamp ring. After loading of primed DNA ATP is hydrolyzed and the beta sliding clamp ring closes (PubMed:9927437)." /translation="MSYQVLARKWRPQTFADVVGQEHVLTALANGLSLGRIHHAYLFSGTRGVGKTSIARLLAKGLNCETGITATPCGVCDNCREIEQGRFVDLIEIDAASRTKVEDTRDLLDNVQYAPARGRFKVYLIDEVHMLSRHSFNALLKTLEEPPEHVKFLLATTDPQKLPVTILSRCLQFHLKALDVEQIRHQLEHILNEEHIAHEPRALQLLARAAEGSLRDALSLTDQAIASGDGQVSTQAVSAMLGTLDDDQALSLVEAMVEANGERVMALINEAAARGIEWEALLVEMLGLLHRIAMVQLSPAALGNDMAAIELRMRELARTIPPTDIQLYYQTLLIGRKELPYAPDRRMGVEMTLLRALAFHPRMPLPEPEVPRQSFAPVAPTAVMTPTQVPPQPQSAPQQAPTVPLPETTSQVLAARQQLQRVQGATKAKKSEPAAATRARPVNNAALERLASVTDRVQARPVPSALEKAPAKKEAYRWKATTPVMQQKEVVATPKALKKALEHEKTPELAAKLAAEAIERDPWAAQVSQLSLPKLVEQVALNAWKEESDNAVCLHLRSSQRHLNNRGAQQKLAEALSMLKGSTVELTIVEDDNPAVRTPLEWRQAIYEEKLAQARESIIADNNIQTLRRFFDAELDEESIRPI" primer_bind complement(90..106) /label="M13 rev" /note="common sequencing primer, one of multiple similar variants" rep_origin complement(173..761) /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(952..1812) /label="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*" promoter complement(1813..1917) /label="bla promoter" /note="ampicillin resistance gene promoter" misc_feature 3996..4055 /label="FreeGenes Barcode" /note="FreeGenes Barcode. Defined as I-SceI site flanked by 21 base pair random sequences." ORIGIN 1 CGCTGAGGTG TCAATCGTCG GAGCCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC 61 TAAACGGGTC TTGAGGGGTT TTTTGCATGG TCATAGCTGT TTCCTGAGAG CTTGGCAGGT 121 GATGACACAC ATTAACAAAT TTCGTGAGGA GTCTCCAGAA GAATGCCATT AATTTCCATA 181 GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC 241 CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG 301 TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC 361 TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG 421 GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC 481 TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA 541 TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG 601 GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA 661 AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG 721 TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGGCCTA CTATTAGCAA 781 CAACGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA 841 AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA 901 ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAAACT TGGTCTGACA GTTACCAATG 961 CTTAATCAGT GAGGCACCTA TCTCAGCGAT CTGTCTATTT CGTTCATCCA TAGTTGCCTG 1021 ACTCCCCGTC GTGTAGATAA CTACGATACG GGAGGGCTTA CCATCTGGCC CCAGTGCTGC 1081 AATGATACCG CGAGAACCAC GCTCACCGGC TCCAGATTTA TCAGCAATAA ACCAGCCAGC 1141 CGGAAGGGCC GAGCGCAGAA GTGGTCCTGC AACTTTATCC GCCTCCATCC AGTCTATTAA 1201 TTGTTGCCGG GAAGCTAGAG TAAGTAGTTC GCCAGTTAAT AGTTTGCGCA ACGTTGTTGC 1261 CATTGCTACA GGCATCGTGG TGTCACGCTC GTCGTTTGGT ATGGCTTCAT TCAGCTCCGG 1321 TTCCCAACGA TCAAGGCGAG TTACATGATC CCCCATGTTG TGCAAAAAAG CGGTTAGCTC 1381 CTTCGGTCCT CCGATCGTTG TCAGAAGTAA GTTGGCCGCA GTGTTATCAC TCATGGTTAT 1441 GGCAGCACTG CATAATTCTC TTACTGTCAT GCCATCCGTA AGATGCTTTT CTGTGACTGG 1501 TGAGTACTCA ACCAAGTCAT TCTGAGAATA GTGTATGCGG CGACCGAGTT GCTCTTGCCC 1561 GGCGTCAATA CGGGATAATA CCGCGCCACA TAGCAGAACT TTAAAAGTGC TCATCATTGG 1621 AAAACGTTCT TCGGGGCGAA AACTCTCAAG GATCTTACCG CTGTTGAGAT CCAGTTCGAT 1681 GTAACCCACT CGTGCACCCA ACTGATCTTC AGCATCTTTT ACTTTCACCA GCGTTTCTGG 1741 GTGAGCAAAA ACAGGAAGGC AAAATGCCGC AAAAAAGGGA ATAAGGGCGA CACGGAAATG 1801 TTGAATACTC ATACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT 1861 CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC 1921 CTGCACCAGT CAGTAAAACG ACGGCCAGTA GTCAAAAGCC TCCGACCGGA GGCTTTTGAC 1981 TTGGTTCAGG TGGAGTGGGA GAAAGCACTC TTAGGCCTCT GGGTCTCTAA TGTCATACCA 2041 GGTCCTAGCA CGTAAATGGC GTCCGCAGAC CTTCGCAGAC GTCGTCGGAC AGGAACACGT 2101 CCTAACCGCA CTAGCAAACG GACTATCACT AGGACGTATC CACCACGCAT ACCTATTCTC 2161 AGGAACCCGT GGAGTCGGAA AAACCTCAAT CGCACGTCTA CTAGCAAAAG GACTAAACTG 2221 CGAAACCGGA ATCACCGCAA CCCCGTGCGG AGTCTGCGAC AACTGCCGTG AAATCGAACA 2281 GGGACGTTTC GTCGACCTAA TCGAAATCGA CGCAGCATCA CGTACCAAAG TCGAAGACAC 2341 CCGTGACCTA CTAGACAACG TCCAGTACGC ACCGGCACGT GGACGTTTCA AAGTCTACCT 2401 AATCGACGAA GTCCACATGC TATCACGTCA CTCATTCAAC GCACTACTAA AAACCCTAGA 2461 AGAACCGCCG GAACACGTCA AATTCCTACT AGCAACCACC GACCCGCAGA AACTACCGGT 2521 CACCATCCTA TCACGTTGCC TACAGTTCCA CCTAAAAGCA CTAGACGTCG AACAGATCCG 2581 TCACCAGCTA GAACACATCC TAAACGAAGA ACACATCGCA CACGAACCGC GTGCACTACA 2641 GCTACTAGCA CGTGCAGCAG AAGGATCACT ACGTGACGCA CTATCACTAA CCGACCAGGC 2701 AATCGCATCA GGAGACGGAC AGGTCTCAAC CCAGGCAGTC TCAGCAATGC TAGGAACCCT 2761 AGACGACGAC CAGGCACTAT CACTAGTCGA AGCAATGGTC GAAGCAAACG GAGAACGTGT 2821 CATGGCACTA ATCAACGAAG CAGCAGCACG TGGAATCGAA TGGGAAGCAC TACTAGTCGA 2881 AATGCTAGGA CTACTACACC GTATCGCAAT GGTCCAGCTA TCACCGGCAG CACTAGGAAA 2941 CGACATGGCA GCAATCGAAC TACGTATGCG TGAACTAGCA CGTACCATCC CGCCGACCGA 3001 CATCCAGCTA TACTACCAGA CCCTACTAAT CGGACGTAAA GAACTACCGT ACGCACCGGA 3061 CCGTCGTATG GGAGTCGAAA TGACCCTACT ACGTGCACTA GCATTCCACC CGCGTATGCC 3121 GCTACCGGAA CCGGAAGTCC CGCGTCAGTC ATTCGCACCG GTCGCACCGA CCGCAGTCAT 3181 GACCCCGACC CAGGTCCCGC CGCAGCCGCA GTCAGCACCG CAGCAGGCAC CGACCGTCCC 3241 GCTACCGGAA ACCACCTCAC AGGTCCTAGC AGCACGTCAG CAGCTACAGC GTGTCCAGGG 3301 AGCAACCAAA GCAAAAAAAT CAGAACCGGC AGCAGCAACC CGTGCACGTC CGGTCAACAA 3361 CGCAGCACTA GAACGTCTAG CATCAGTCAC CGACCGTGTC CAGGCACGTC CGGTCCCGTC 3421 AGCACTAGAA AAAGCACCGG CAAAAAAAGA AGCATACCGT TGGAAAGCAA CCACCCCGGT 3481 CATGCAGCAG AAAGAAGTCG TCGCAACCCC GAAAGCACTA AAAAAAGCAC TAGAACACGA 3541 AAAAACCCCG GAACTAGCAG CAAAACTAGC AGCAGAAGCA ATCGAACGTG ACCCGTGGGC 3601 AGCACAGGTC TCACAGCTAT CACTACCGAA ACTAGTCGAA CAGGTCGCAC TAAACGCATG 3661 GAAAGAAGAA TCAGACAACG CAGTCTGCCT ACACCTACGT TCATCACAGC GTCACCTAAA 3721 CAACCGTGGA GCACAGCAGA AACTAGCAGA AGCACTATCA ATGCTAAAAG GATCAACCGT 3781 CGAACTAACC ATCGTCGAAG ACGACAACCC GGCAGTCCGT ACCCCGCTAG AATGGCGTCA 3841 GGCAATCTAC GAAGAAAAAC TAGCACAGGC ACGTGAATCA ATCATCGCAG ACAACAACAT 3901 CCAGACCCTA CGTCGTTTCT TCGACGCAGA ACTAGACGAA GAATCAATCC GTCCGATCTG 3961 AAGAGCTTCG AGACCAGTAT TGCCGGACTA AACCCCGGAA ATCTGTACTG CTCAAGTAGG 4021 GATAACAGGG TAATGTTTCG CGCTTTCAAT TCCCC //