LOCUS       Exported     3509 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..3509
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      38..85
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     1933..1949
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      1950..1981
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             2030..3415
                     /label="cysS"
                     /note=""
                     /translation="MLKIFNTLTRQKEEFKPIHAGEVGMYVCGITVYDLCHIGHGRTFVAFDVVARYLRFLGYKLKYVRNITDIDDKIIKRANENGESFVAMVDRMIAEMHKDFDALNILRPDMEPRATHHIAEIIELTEQLIAKGHAYVADNGDVMFDVPTDPTYGVLSRQDLDQLQAGARVDVVDDKRNPMDFVLWKMSKEGEPSWPSPWGAGRPGWHIECSAMNCKQLGNHFDIHGGGSDLMFPHHENEIAQSTCAHDGQYVNYWMHSGMVMVDREKMSKSLGNFFTVRDVLKYYDAETVRYFLMSGHYRSQLNYSEENLKQARAALERLYTALRGTDKTVAPAGGEAFEARFIEAMDDDFNTPEAYSVLFDMAREVNRLKAEDMAAANAMASHLRKLSAVLGLLEQEPEAFLQSGAQADDSEVAEIEALIQQRLDARKAKDWAAADAARDRLNEMGIVLEDGPQGTTWRRK"
     primer_bind     complement(90..106)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(173..761)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(952..1812)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(1813..1917)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
     misc_feature    3450..3509
                     /label="FreeGenes Barcode"
                     /note="FreeGenes Barcode. Defined as I-SceI site flanked by 21 base pair random sequences."
ORIGIN
        1 CGCTGAGGTG TCAATCGTCG GAGCCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC
       61 TAAACGGGTC TTGAGGGGTT TTTTGCATGG TCATAGCTGT TTCCTGAGAG CTTGGCAGGT
      121 GATGACACAC ATTAACAAAT TTCGTGAGGA GTCTCCAGAA GAATGCCATT AATTTCCATA
      181 GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC
      241 CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG
      301 TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC
      361 TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG
      421 GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC
      481 TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA
      541 TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG
      601 GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA
      661 AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG
      721 TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGGCCTA CTATTAGCAA
      781 CAACGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA
      841 AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA
      901 ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAAACT TGGTCTGACA GTTACCAATG
      961 CTTAATCAGT GAGGCACCTA TCTCAGCGAT CTGTCTATTT CGTTCATCCA TAGTTGCCTG
     1021 ACTCCCCGTC GTGTAGATAA CTACGATACG GGAGGGCTTA CCATCTGGCC CCAGTGCTGC
     1081 AATGATACCG CGAGAACCAC GCTCACCGGC TCCAGATTTA TCAGCAATAA ACCAGCCAGC
     1141 CGGAAGGGCC GAGCGCAGAA GTGGTCCTGC AACTTTATCC GCCTCCATCC AGTCTATTAA
     1201 TTGTTGCCGG GAAGCTAGAG TAAGTAGTTC GCCAGTTAAT AGTTTGCGCA ACGTTGTTGC
     1261 CATTGCTACA GGCATCGTGG TGTCACGCTC GTCGTTTGGT ATGGCTTCAT TCAGCTCCGG
     1321 TTCCCAACGA TCAAGGCGAG TTACATGATC CCCCATGTTG TGCAAAAAAG CGGTTAGCTC
     1381 CTTCGGTCCT CCGATCGTTG TCAGAAGTAA GTTGGCCGCA GTGTTATCAC TCATGGTTAT
     1441 GGCAGCACTG CATAATTCTC TTACTGTCAT GCCATCCGTA AGATGCTTTT CTGTGACTGG
     1501 TGAGTACTCA ACCAAGTCAT TCTGAGAATA GTGTATGCGG CGACCGAGTT GCTCTTGCCC
     1561 GGCGTCAATA CGGGATAATA CCGCGCCACA TAGCAGAACT TTAAAAGTGC TCATCATTGG
     1621 AAAACGTTCT TCGGGGCGAA AACTCTCAAG GATCTTACCG CTGTTGAGAT CCAGTTCGAT
     1681 GTAACCCACT CGTGCACCCA ACTGATCTTC AGCATCTTTT ACTTTCACCA GCGTTTCTGG
     1741 GTGAGCAAAA ACAGGAAGGC AAAATGCCGC AAAAAAGGGA ATAAGGGCGA CACGGAAATG
     1801 TTGAATACTC ATACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT
     1861 CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC
     1921 CTGCACCAGT CAGTAAAACG ACGGCCAGTA GTCAAAAGCC TCCGACCGGA GGCTTTTGAC
     1981 TTGGTTCAGG TGGAGTGGGA GAAAGCACTC TTAGGCCTCT GGGTCTCTAA TGCTAAAAAT
     2041 CTTCAACACC CTAACCCGTC AGAAAGAAGA ATTCAAACCG ATCCACGCAG GAGAAGTCGG
     2101 AATGTACGTC TGCGGAATCA CCGTCTACGA CCTATGCCAC ATCGGACACG GACGTACCTT
     2161 CGTCGCATTC GACGTCGTCG CACGTTACCT ACGTTTCCTA GGATACAAAC TAAAATACGT
     2221 CCGTAACATC ACCGACATCG ACGACAAAAT CATCAAACGT GCAAACGAAA ACGGAGAATC
     2281 ATTCGTCGCA ATGGTCGACC GTATGATCGC AGAAATGCAC AAAGACTTCG ACGCACTAAA
     2341 CATCCTACGT CCGGACATGG AACCGCGTGC AACCCACCAC ATCGCAGAAA TCATCGAACT
     2401 AACCGAACAG CTAATCGCAA AAGGACACGC ATACGTCGCA GACAACGGAG ACGTCATGTT
     2461 CGACGTCCCG ACCGACCCGA CCTACGGAGT CCTATCACGT CAGGACCTAG ACCAGCTACA
     2521 GGCAGGAGCA CGTGTCGACG TCGTCGACGA CAAACGTAAC CCGATGGACT TCGTCCTATG
     2581 GAAAATGTCA AAAGAAGGAG AACCGTCATG GCCGTCACCG TGGGGAGCAG GACGTCCGGG
     2641 ATGGCACATC GAATGCTCAG CAATGAACTG CAAACAGCTA GGAAACCACT TCGACATCCA
     2701 CGGAGGAGGA TCAGACCTAA TGTTCCCGCA CCACGAAAAC GAAATCGCAC AGTCAACCTG
     2761 CGCACACGAC GGACAGTACG TCAACTACTG GATGCACTCA GGAATGGTCA TGGTCGACCG
     2821 TGAAAAAATG TCAAAATCAC TAGGAAACTT CTTCACCGTC CGTGACGTCC TAAAATACTA
     2881 CGACGCAGAA ACCGTCCGTT ACTTCCTAAT GTCAGGACAC TACCGTTCAC AGCTAAACTA
     2941 CTCAGAAGAA AACCTAAAAC AGGCACGTGC AGCACTAGAA CGTCTATACA CCGCACTACG
     3001 TGGAACCGAC AAAACCGTCG CACCGGCAGG AGGAGAAGCA TTCGAAGCAC GTTTCATCGA
     3061 AGCAATGGAC GACGACTTCA ACACCCCGGA AGCATACTCA GTCCTATTCG ACATGGCACG
     3121 TGAAGTCAAC CGTCTAAAAG CAGAAGACAT GGCAGCAGCA AACGCAATGG CATCACACCT
     3181 ACGTAAACTA TCAGCAGTCC TAGGACTACT AGAACAGGAA CCGGAAGCAT TCCTACAGTC
     3241 AGGAGCACAG GCAGACGACT CAGAAGTCGC AGAAATCGAA GCACTAATCC AGCAGCGTCT
     3301 AGACGCACGT AAAGCAAAAG ACTGGGCAGC AGCAGACGCA GCACGTGACC GTCTAAACGA
     3361 AATGGGAATC GTCCTAGAAG ACGGACCGCA GGGAACCACC TGGCGTCGTA AATGAAGAGC
     3421 TTCGAGACCA AGACGATCCG AGCCATTACC TCCAGAACCT CCATCCCCCT TAGGGATAAC
     3481 AGGGTAATCG CAGCTGCTAT ATTACCGCT
//