LOCUS       Exported     4025 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..4025
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      38..85
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
                     /label="T7 terminator"
     primer_bind     1933..1949
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      1950..1981
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             2030..3931
                     /label="mrdA"
                     /note="Catalyzes cross-linking of the peptidoglycan cell wall (PubMed:3009484). Responsible for the determination of the rod shape of the cell (PubMed:1103132). Is probably required for lateral peptidoglycan synthesis and maintenance of the correct diameter during lateral and centripetal growth (PubMed:12519203)."
                     /translation="MKLQNSFRDYTAESALFVRRALVAFLGILLLTGVLIANLYNLQIVRFTDYQTRSNENRIKLVPIAPSRGIIYDRNGIPLALNRTIYQIEMMPEKVDNVQQTLDALRSVVDLTDDDIAAFRKERARSHRFTSIPVKTNLTEVQVARFAVNQYRFPGVEVKGYKRRYYPYGSALTHVIGYVSKINDKDVERLNNDGKLANYAATHDIGKLGIERYYEDVLHGQTGYEEVEVNNRGRVIRQLKEVPPQAGHDIYLTLDLKLQQYIETLLAGSRAAVVVTDPRTGGVLALVSTPSYDPNLFVDGISSKDYSALLNDPNTPLVNRATQGVYPPASTVKPYVAVSALSAGVITRNTTLFDPGWWQLPGSEKRYRDWKKWGHGRLNVTRSLEESADTFFYQVAYDMGIDRLSEWMGKFGYGHYTGIDLAEERSGNMPTREWKQKRFKKPWYQGDTIPVGIGQGYWTATPIQMSKALMILINDGIVKVPHLLMSTAEDGKQVPWVQPHEPPVGDIHSGYWELAKDGMYGVANRPNGTAHKYFASAPYKIAAKSGTAQVFGLKANETYNAHKIAERLRDHKLMTAFAPYNNPQVAVAMILENGGAGPAVGTLMRQILDHIMLGDNNTDLPAENPAVAAAEDH"
     primer_bind     complement(90..106)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(173..761)
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
                     /label="ori"
     CDS             complement(952..1812)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(1813..1917)
                     /note="ampicillin resistance gene promoter"
                     /label="bla promoter"
     misc_feature    3966..4025
                     /label="FreeGenes Barcode"
                     /note="FreeGenes Barcode. Defined as I-SceI site flanked by 21 base pair random sequences."
ORIGIN
        1 CGCTGAGGTG TCAATCGTCG GAGCCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC
       61 TAAACGGGTC TTGAGGGGTT TTTTGCATGG TCATAGCTGT TTCCTGAGAG CTTGGCAGGT
      121 GATGACACAC ATTAACAAAT TTCGTGAGGA GTCTCCAGAA GAATGCCATT AATTTCCATA
      181 GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC
      241 CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG
      301 TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC
      361 TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG
      421 GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC
      481 TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA
      541 TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG
      601 GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA
      661 AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG
      721 TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGGCCTA CTATTAGCAA
      781 CAACGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA
      841 AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA
      901 ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAAACT TGGTCTGACA GTTACCAATG
      961 CTTAATCAGT GAGGCACCTA TCTCAGCGAT CTGTCTATTT CGTTCATCCA TAGTTGCCTG
     1021 ACTCCCCGTC GTGTAGATAA CTACGATACG GGAGGGCTTA CCATCTGGCC CCAGTGCTGC
     1081 AATGATACCG CGAGAACCAC GCTCACCGGC TCCAGATTTA TCAGCAATAA ACCAGCCAGC
     1141 CGGAAGGGCC GAGCGCAGAA GTGGTCCTGC AACTTTATCC GCCTCCATCC AGTCTATTAA
     1201 TTGTTGCCGG GAAGCTAGAG TAAGTAGTTC GCCAGTTAAT AGTTTGCGCA ACGTTGTTGC
     1261 CATTGCTACA GGCATCGTGG TGTCACGCTC GTCGTTTGGT ATGGCTTCAT TCAGCTCCGG
     1321 TTCCCAACGA TCAAGGCGAG TTACATGATC CCCCATGTTG TGCAAAAAAG CGGTTAGCTC
     1381 CTTCGGTCCT CCGATCGTTG TCAGAAGTAA GTTGGCCGCA GTGTTATCAC TCATGGTTAT
     1441 GGCAGCACTG CATAATTCTC TTACTGTCAT GCCATCCGTA AGATGCTTTT CTGTGACTGG
     1501 TGAGTACTCA ACCAAGTCAT TCTGAGAATA GTGTATGCGG CGACCGAGTT GCTCTTGCCC
     1561 GGCGTCAATA CGGGATAATA CCGCGCCACA TAGCAGAACT TTAAAAGTGC TCATCATTGG
     1621 AAAACGTTCT TCGGGGCGAA AACTCTCAAG GATCTTACCG CTGTTGAGAT CCAGTTCGAT
     1681 GTAACCCACT CGTGCACCCA ACTGATCTTC AGCATCTTTT ACTTTCACCA GCGTTTCTGG
     1741 GTGAGCAAAA ACAGGAAGGC AAAATGCCGC AAAAAAGGGA ATAAGGGCGA CACGGAAATG
     1801 TTGAATACTC ATACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT
     1861 CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC
     1921 CTGCACCAGT CAGTAAAACG ACGGCCAGTA GTCAAAAGCC TCCGACCGGA GGCTTTTGAC
     1981 TTGGTTCAGG TGGAGTGGGA GAAACACGTG GCAAACATTC CGGTCTCTAA TGAAACTACA
     2041 GAACTCATTC CGTGACTACA CCGCAGAATC AGCACTATTC GTCCGTCGTG CACTAGTCGC
     2101 ATTCCTAGGA ATCCTACTAC TAACCGGAGT CCTAATCGCA AACCTATACA ACCTACAGAT
     2161 CGTCCGTTTC ACCGACTACC AGACCCGTTC AAACGAAAAC CGTATCAAAC TAGTCCCGAT
     2221 CGCACCGTCA CGTGGAATCA TCTACGACCG TAACGGAATC CCGCTAGCAC TAAACCGTAC
     2281 CATCTACCAG ATCGAAATGA TGCCGGAAAA AGTCGACAAC GTCCAGCAGA CCCTAGACGC
     2341 ACTACGTTCA GTCGTCGACC TAACCGACGA CGACATCGCA GCATTCCGTA AAGAACGTGC
     2401 ACGTTCACAC CGTTTCACCT CAATCCCGGT CAAAACCAAC CTAACCGAAG TCCAGGTCGC
     2461 ACGTTTCGCA GTCAACCAGT ACCGTTTCCC GGGAGTCGAA GTCAAAGGAT ACAAACGTCG
     2521 TTACTACCCG TACGGATCAG CACTAACCCA CGTCATCGGA TACGTCTCAA AAATCAACGA
     2581 CAAAGACGTC GAACGTCTAA ACAACGACGG AAAACTAGCA AACTACGCAG CAACCCACGA
     2641 CATCGGAAAA CTAGGAATCG AACGTTACTA CGAAGACGTC CTACACGGAC AGACCGGATA
     2701 CGAAGAAGTC GAAGTCAACA ACCGTGGACG TGTCATCCGT CAGCTAAAAG AAGTCCCGCC
     2761 GCAGGCAGGA CACGACATCT ACCTAACCCT AGACCTAAAA CTACAGCAGT ACATCGAAAC
     2821 CCTACTAGCA GGATCACGTG CAGCAGTCGT CGTCACCGAC CCGCGTACCG GAGGAGTCCT
     2881 AGCACTAGTC TCAACCCCGT CATACGACCC GAACCTATTC GTCGACGGAA TCTCATCAAA
     2941 AGACTACTCA GCACTACTAA ACGACCCGAA CACCCCGCTA GTCAACCGTG CAACCCAGGG
     3001 AGTCTACCCG CCGGCATCAA CCGTCAAACC GTACGTCGCA GTCTCAGCAC TATCAGCAGG
     3061 AGTCATCACC CGTAACACCA CCCTATTCGA CCCGGGATGG TGGCAGCTAC CGGGATCAGA
     3121 AAAACGTTAC CGTGACTGGA AAAAATGGGG ACACGGACGT CTAAACGTCA CCCGTTCACT
     3181 AGAAGAATCA GCAGACACCT TCTTCTACCA GGTCGCATAC GACATGGGAA TCGACCGTCT
     3241 ATCAGAATGG ATGGGAAAAT TCGGATACGG ACACTACACC GGAATCGACC TAGCAGAAGA
     3301 ACGTTCAGGA AACATGCCGA CCCGTGAATG GAAACAGAAA CGTTTCAAAA AACCGTGGTA
     3361 CCAGGGAGAC ACCATCCCGG TCGGAATCGG ACAGGGATAC TGGACCGCAA CCCCGATCCA
     3421 GATGTCAAAA GCACTAATGA TCCTAATCAA CGACGGAATC GTCAAAGTCC CGCACCTACT
     3481 AATGTCAACC GCAGAAGACG GAAAACAGGT CCCGTGGGTC CAGCCGCACG AACCGCCGGT
     3541 CGGAGACATC CACTCAGGAT ACTGGGAACT AGCAAAAGAC GGAATGTACG GAGTCGCAAA
     3601 CCGTCCGAAC GGAACCGCAC ACAAATACTT CGCATCAGCA CCGTACAAAA TCGCAGCAAA
     3661 ATCAGGAACC GCACAGGTCT TCGGACTAAA AGCAAACGAA ACCTACAACG CACACAAAAT
     3721 CGCAGAACGT CTACGTGACC ACAAACTAAT GACCGCATTC GCACCGTACA ACAACCCGCA
     3781 GGTCGCAGTC GCAATGATCC TAGAAAACGG AGGAGCAGGA CCGGCAGTCG GAACCCTAAT
     3841 GCGTCAGATC CTAGACCACA TCATGCTAGG AGACAACAAC ACCGACCTAC CGGCAGAAAA
     3901 CCCGGCAGTC GCAGCAGCAG AAGACCACTG AAGAGCTTCG AGACCACGAG ATGATGCACC
     3961 GATAGCTCAT ATAGACATAC TTACTATAGG GATAACAGGG TAATGCGAAA CCGCTGCTCG
     4021 TGCTA
//