LOCUS       Exported     3788 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..3788
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      38..85
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     1933..1949
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      1950..1981
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             100..3787
                     /label="glnS"
                     /note=""
                     /translation="MSEAEARPTNFIRQIIDEDLASGKHTTVHTRFPPEPNGYLHIGHAKSICLNFGIAQDYKGQCNLRFDDTNPVKEDIEYVESIKNDVEWLGFHWSGNVRYSSDYFDQLHAYAIELINKGLAYVDELTPEQIREYRGTLTQPGKNSPYRDRSVEENLALFEKMRAGGFEEGKACLRAKIDMASPFIVMRDPVLYRIKFAEHHQTGNKWCIYPMYDFTHCISDALEGITHSLCTLEFQDNRRLYDWVLDNITIPVHPRQYEFSRLNLEYTVMSKRKLNLLVTDKHVEGWDDPRMPTISGLRRRGYTAASIREFCKRIGVTKQDNTIEMASLESCIREDLNENAPRAMAVIDPVKLVIENYQGEGEMVTMPNHPNKPEMGSRQVPFSGEIWIDRADFREEANKQYKRLVLGKEVRLRNAYVIKAERVEKDAEGNITTIFCTYDADTLSKDPADGRKVKGVIHWVSAAHALPVEIRLYDRLFSVPNPGAADDFLSVINPESLVIKQGFAEPSLKDAVAGKAFQFEREGYFCLDSRHSTAEKPVFNRTVGLRDTWAKVGE"
     primer_bind     complement(90..106)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(173..761)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(952..1812)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(1813..1917)
                     /note="ampicillin resistance gene promoter"
                     /label="bla promoter"
     misc_feature    3729..3788
                     /label="FreeGenes Barcode"
                     /note="FreeGenes Barcode. Defined as I-SceI site flanked by 21 base pair random sequences."
ORIGIN
        1 CGCTGAGGTG TCAATCGTCG GAGCCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC
       61 TAAACGGGTC TTGAGGGGTT TTTTGCATGG TCATAGCTGT TTCCTGAGAG CTTGGCAGGT
      121 GATGACACAC ATTAACAAAT TTCGTGAGGA GTCTCCAGAA GAATGCCATT AATTTCCATA
      181 GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC
      241 CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG
      301 TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC
      361 TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG
      421 GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC
      481 TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA
      541 TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG
      601 GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA
      661 AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG
      721 TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGGCCTA CTATTAGCAA
      781 CAACGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA
      841 AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA
      901 ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAAACT TGGTCTGACA GTTACCAATG
      961 CTTAATCAGT GAGGCACCTA TCTCAGCGAT CTGTCTATTT CGTTCATCCA TAGTTGCCTG
     1021 ACTCCCCGTC GTGTAGATAA CTACGATACG GGAGGGCTTA CCATCTGGCC CCAGTGCTGC
     1081 AATGATACCG CGAGAACCAC GCTCACCGGC TCCAGATTTA TCAGCAATAA ACCAGCCAGC
     1141 CGGAAGGGCC GAGCGCAGAA GTGGTCCTGC AACTTTATCC GCCTCCATCC AGTCTATTAA
     1201 TTGTTGCCGG GAAGCTAGAG TAAGTAGTTC GCCAGTTAAT AGTTTGCGCA ACGTTGTTGC
     1261 CATTGCTACA GGCATCGTGG TGTCACGCTC GTCGTTTGGT ATGGCTTCAT TCAGCTCCGG
     1321 TTCCCAACGA TCAAGGCGAG TTACATGATC CCCCATGTTG TGCAAAAAAG CGGTTAGCTC
     1381 CTTCGGTCCT CCGATCGTTG TCAGAAGTAA GTTGGCCGCA GTGTTATCAC TCATGGTTAT
     1441 GGCAGCACTG CATAATTCTC TTACTGTCAT GCCATCCGTA AGATGCTTTT CTGTGACTGG
     1501 TGAGTACTCA ACCAAGTCAT TCTGAGAATA GTGTATGCGG CGACCGAGTT GCTCTTGCCC
     1561 GGCGTCAATA CGGGATAATA CCGCGCCACA TAGCAGAACT TTAAAAGTGC TCATCATTGG
     1621 AAAACGTTCT TCGGGGCGAA AACTCTCAAG GATCTTACCG CTGTTGAGAT CCAGTTCGAT
     1681 GTAACCCACT CGTGCACCCA ACTGATCTTC AGCATCTTTT ACTTTCACCA GCGTTTCTGG
     1741 GTGAGCAAAA ACAGGAAGGC AAAATGCCGC AAAAAAGGGA ATAAGGGCGA CACGGAAATG
     1801 TTGAATACTC ATACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT
     1861 CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC
     1921 CTGCACCAGT CAGTAAAACG ACGGCCAGTA GTCAAAAGCC TCCGACCGGA GGCTTTTGAC
     1981 TTGGTTCAGG TGGAGTGGGA GAAACCGGAG CCATACAGTA CGGTCTCTAA TGTCAGAAGC
     2041 AGAAGCACGT CCGACCAACT TCATCCGTCA GATCATCGAC GAAGACCTAG CATCAGGAAA
     2101 ACACACCACC GTCCACACCC GTTTCCCGCC GGAACCGAAC GGATACCTAC ACATCGGACA
     2161 CGCAAAATCA ATCTGCCTAA ACTTCGGAAT CGCACAGGAC TACAAAGGAC AGTGCAACCT
     2221 ACGTTTCGAC GACACCAACC CGGTCAAAGA AGACATCGAA TACGTCGAAT CAATCAAAAA
     2281 CGACGTCGAA TGGCTAGGAT TCCACTGGTC AGGAAACGTC CGTTACTCAT CAGACTACTT
     2341 CGACCAGCTA CACGCATACG CAATCGAACT AATCAACAAA GGACTAGCAT ACGTCGACGA
     2401 ACTAACCCCG GAACAGATCC GTGAATACCG TGGAACCCTA ACCCAGCCGG GAAAAAACTC
     2461 ACCGTACCGT GACCGTTCAG TCGAAGAAAA CCTAGCACTA TTCGAAAAAA TGCGTGCAGG
     2521 AGGATTCGAA GAAGGAAAAG CATGCCTACG TGCAAAAATC GACATGGCAT CACCGTTCAT
     2581 CGTCATGCGT GACCCGGTCC TATACCGTAT CAAATTCGCA GAACACCACC AGACCGGAAA
     2641 CAAATGGTGC ATCTACCCGA TGTACGACTT CACCCACTGC ATCTCAGACG CACTAGAAGG
     2701 AATCACCCAC TCACTATGCA CCCTAGAATT CCAGGACAAC CGTCGTCTAT ACGACTGGGT
     2761 CCTAGACAAC ATCACCATCC CGGTCCACCC GCGTCAGTAC GAATTCTCAC GTCTAAACCT
     2821 AGAATACACC GTCATGTCAA AACGTAAACT AAACCTACTA GTCACCGACA AACACGTCGA
     2881 AGGATGGGAC GACCCGCGTA TGCCGACCAT CTCAGGACTA CGTCGTCGTG GATACACCGC
     2941 AGCATCAATC CGTGAATTCT GCAAACGTAT CGGAGTCACC AAACAGGACA ACACCATCGA
     3001 AATGGCATCA CTAGAATCAT GCATCCGTGA AGACCTAAAC GAAAACGCAC CGCGTGCAAT
     3061 GGCAGTCATC GACCCGGTCA AACTAGTCAT CGAAAACTAC CAGGGAGAAG GAGAAATGGT
     3121 CACCATGCCG AACCACCCGA ACAAACCGGA AATGGGATCA CGTCAGGTCC CGTTCTCAGG
     3181 AGAAATCTGG ATCGACCGTG CAGACTTCCG TGAAGAAGCA AACAAACAGT ACAAACGTCT
     3241 AGTCCTAGGA AAAGAAGTCC GTCTACGTAA CGCATACGTC ATCAAAGCAG AACGTGTCGA
     3301 AAAAGACGCA GAAGGAAACA TCACCACCAT CTTCTGCACC TACGACGCAG ACACCCTATC
     3361 AAAAGACCCG GCAGACGGAC GTAAAGTCAA AGGAGTCATC CACTGGGTCT CAGCAGCACA
     3421 CGCACTACCG GTCGAAATCC GTCTATACGA CCGTCTATTC TCAGTCCCGA ACCCGGGAGC
     3481 AGCAGACGAC TTCCTATCAG TCATCAACCC GGAATCACTA GTCATCAAAC AGGGATTCGC
     3541 AGAACCGTCA CTAAAAGACG CAGTCGCAGG AAAAGCATTC CAGTTCGAAC GTGAAGGATA
     3601 CTTCTGCCTA GACTCACGTC ACTCAACCGC AGAAAAACCG GTCTTCAACC GTACCGTCGG
     3661 ACTACGTGAC ACCTGGGCAA AAGTCGGAGA ATGAAGAGCT TCGAGACCAT GCTAGCTGGA
     3721 ACTATCGGTT AATAGAACCC GTATCCGATT AGGGATAACA GGGTAATCGG TTGCTCACGA
     3781 CCCGGTTG
//