LOCUS Exported 3692 bp DNA circular SYN 24-JUL-2020 DEFINITION synthetic linear DNA ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2001) AUTHORS Keoni Gandall TITLE Direct Submission JOURNAL Exported from Poly. FEATURES Location/Qualifiers terminator 1..3692 /note="transcription terminator for bacteriophage T7 RNA polymerase" /label="T7 terminator" terminator 38..85 /label="T7 terminator" /note="transcription terminator for bacteriophage T7 RNA polymerase" primer_bind 1933..1949 /label="M13 for" /note="common sequencing primer, one of multiple similar variants" terminator 1950..1981 /label="tonB terminator" /note="bidirectional E. coli tonB-P14 transcription terminator" CDS 100..3691 /translation="MLDIVELSRLQFALTAMYHFLFVPLTLGMAFLLAIMETVYVLSGKQIYKDMTKFWGKLFGINFALGVATGLTMEFQFGTNWSYYSHYVGDIFGAPLAIEGLMAFFLESTFVGLFFFGWDRLGKVQHMCVTWLVALGSNLSALWILVANGWMQNPIASDFNFETMRMEMVSFSELVLNPVAQVKFVHTVASGYVTGAMFILGISAWYMLKGRDFAFAKRSFAIAASFGMAAVLSVIVLGDESGYEMGDVQKTKLAAIEAEWETQPAPAAFTLFGIPDQEEETNKFAIQIPYALGIIATRSVDTPVIGLKELMVQHEERIRNGMKAYSLLEQLRSGSTDQAVRDQFNSMKKDLGYGLLLKRYTPNVADATEAQIQQATKDSIPRVAPLYFAFRIMVACGFLLLAIIALSFWSVIRNRIGEKKWLLRAALYGIPLPWIAVEAGWFVAEYGRQPWAIGEVLPTAVANSSLTAGDLIFSMVLICGLYTLFLVAELFLMFKFARLGPSSLKTGRYHFEQSSTTTQPAR" /label="cydA" /note="A terminal oxidase that produces a proton motive force by the vectorial transfer of protons across the inner membrane. It is the component of the aerobic respiratory chain of E.coli that predominates when cells are grown at low aeration. Generates a proton motive force using protons and electrons from opposite sides of the membrane to generate H(2)O, transferring 1 proton/electron." primer_bind complement(90..106) /label="M13 rev" /note="common sequencing primer, one of multiple similar variants" rep_origin complement(173..761) /label="ori" /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(952..1812) /label="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*" promoter complement(1813..1917) /label="bla promoter" /note="ampicillin resistance gene promoter" misc_feature 3633..3692 /label="FreeGenes Barcode" /note="FreeGenes Barcode. Defined as I-SceI site flanked by 21 base pair random sequences." ORIGIN 1 CGCTGAGGTG TCAATCGTCG GAGCCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC 61 TAAACGGGTC TTGAGGGGTT TTTTGCATGG TCATAGCTGT TTCCTGAGAG CTTGGCAGGT 121 GATGACACAC ATTAACAAAT TTCGTGAGGA GTCTCCAGAA GAATGCCATT AATTTCCATA 181 GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC 241 CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG 301 TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC 361 TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG 421 GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC 481 TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA 541 TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG 601 GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA 661 AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG 721 TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGGCCTA CTATTAGCAA 781 CAACGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA 841 AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA 901 ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAAACT TGGTCTGACA GTTACCAATG 961 CTTAATCAGT GAGGCACCTA TCTCAGCGAT CTGTCTATTT CGTTCATCCA TAGTTGCCTG 1021 ACTCCCCGTC GTGTAGATAA CTACGATACG GGAGGGCTTA CCATCTGGCC CCAGTGCTGC 1081 AATGATACCG CGAGAACCAC GCTCACCGGC TCCAGATTTA TCAGCAATAA ACCAGCCAGC 1141 CGGAAGGGCC GAGCGCAGAA GTGGTCCTGC AACTTTATCC GCCTCCATCC AGTCTATTAA 1201 TTGTTGCCGG GAAGCTAGAG TAAGTAGTTC GCCAGTTAAT AGTTTGCGCA ACGTTGTTGC 1261 CATTGCTACA GGCATCGTGG TGTCACGCTC GTCGTTTGGT ATGGCTTCAT TCAGCTCCGG 1321 TTCCCAACGA TCAAGGCGAG TTACATGATC CCCCATGTTG TGCAAAAAAG CGGTTAGCTC 1381 CTTCGGTCCT CCGATCGTTG TCAGAAGTAA GTTGGCCGCA GTGTTATCAC TCATGGTTAT 1441 GGCAGCACTG CATAATTCTC TTACTGTCAT GCCATCCGTA AGATGCTTTT CTGTGACTGG 1501 TGAGTACTCA ACCAAGTCAT TCTGAGAATA GTGTATGCGG CGACCGAGTT GCTCTTGCCC 1561 GGCGTCAATA CGGGATAATA CCGCGCCACA TAGCAGAACT TTAAAAGTGC TCATCATTGG 1621 AAAACGTTCT TCGGGGCGAA AACTCTCAAG GATCTTACCG CTGTTGAGAT CCAGTTCGAT 1681 GTAACCCACT CGTGCACCCA ACTGATCTTC AGCATCTTTT ACTTTCACCA GCGTTTCTGG 1741 GTGAGCAAAA ACAGGAAGGC AAAATGCCGC AAAAAAGGGA ATAAGGGCGA CACGGAAATG 1801 TTGAATACTC ATACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT 1861 CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC 1921 CTGCACCAGT CAGTAAAACG ACGGCCAGTA GTCAAAAGCC TCCGACCGGA GGCTTTTGAC 1981 TTGGTTCAGG TGGAGTGGGA GAAAGCACTC TTAGGCCTCT GGGTCTCTAA TGCTAGACAT 2041 CGTCGAACTA TCACGTCTAC AGTTCGCACT AACCGCAATG TACCACTTCC TATTCGTCCC 2101 GCTAACCCTA GGAATGGCAT TCCTACTAGC AATCATGGAA ACCGTCTACG TCCTATCAGG 2161 AAAACAGATC TACAAAGACA TGACCAAATT CTGGGGAAAA CTATTCGGAA TCAACTTCGC 2221 ACTAGGAGTC GCAACCGGAC TAACCATGGA ATTCCAGTTC GGAACCAACT GGTCATACTA 2281 CTCACACTAC GTCGGAGACA TCTTCGGAGC ACCGCTAGCA ATCGAAGGAC TAATGGCATT 2341 CTTCCTAGAA TCAACCTTCG TCGGACTATT CTTCTTCGGA TGGGACCGTC TAGGAAAAGT 2401 CCAGCACATG TGCGTCACCT GGCTAGTCGC ACTAGGATCA AACCTATCAG CACTATGGAT 2461 CCTAGTCGCA AACGGATGGA TGCAGAACCC GATCGCATCA GACTTCAACT TCGAAACCAT 2521 GCGTATGGAA ATGGTCTCAT TCTCAGAACT AGTCCTAAAC CCGGTCGCAC AGGTCAAATT 2581 CGTCCACACC GTCGCATCAG GATACGTCAC CGGAGCAATG TTCATCCTAG GAATCTCAGC 2641 ATGGTACATG CTAAAAGGAC GTGACTTCGC ATTCGCAAAA CGTTCATTCG CAATCGCAGC 2701 ATCATTCGGA ATGGCAGCAG TCCTATCAGT CATCGTCCTA GGAGACGAAT CAGGATACGA 2761 AATGGGAGAC GTCCAGAAAA CCAAACTAGC AGCAATCGAA GCAGAATGGG AAACCCAGCC 2821 GGCACCGGCA GCATTCACCC TATTCGGAAT CCCGGACCAG GAAGAAGAAA CCAACAAATT 2881 CGCAATCCAG ATCCCGTACG CACTAGGAAT CATCGCAACC CGTTCAGTCG ACACCCCGGT 2941 CATCGGACTA AAAGAACTAA TGGTCCAGCA CGAAGAACGT ATCCGTAACG GAATGAAAGC 3001 ATACTCACTA CTAGAACAGC TACGTTCAGG ATCAACCGAC CAGGCAGTCC GTGACCAGTT 3061 CAACTCAATG AAAAAAGACC TAGGATACGG ACTACTACTA AAACGTTACA CCCCGAACGT 3121 CGCAGACGCA ACCGAAGCAC AGATCCAGCA GGCAACCAAA GACTCAATCC CGCGTGTCGC 3181 ACCGCTATAC TTCGCATTCC GTATCATGGT CGCATGCGGA TTCCTACTAC TAGCAATCAT 3241 CGCACTATCA TTCTGGTCAG TCATCCGTAA CCGTATCGGA GAAAAAAAAT GGCTACTACG 3301 TGCAGCACTA TACGGAATCC CGCTACCGTG GATCGCAGTC GAAGCAGGAT GGTTCGTCGC 3361 AGAATACGGA CGTCAGCCGT GGGCAATCGG AGAAGTCCTA CCGACCGCAG TCGCAAACTC 3421 ATCACTAACC GCAGGAGACC TAATCTTCTC AATGGTCCTA ATCTGCGGAC TATACACCCT 3481 ATTCCTAGTC GCAGAACTAT TCCTAATGTT CAAATTCGCA CGTCTAGGAC CGTCATCACT 3541 AAAAACCGGA CGTTACCACT TCGAACAGTC ATCAACCACC ACCCAGCCGG CACGTTGAAG 3601 AGCTTCGAGA CCAGCTATGA TCCCGGTGTA ACGCCCGACG TGAGAAGTCC AGGTAGGGAT 3661 AACAGGGTAA TTAGTTTTAA GAATGCCTAC GA //