LOCUS       Exported     3692 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..3692
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
                     /label="T7 terminator"
     terminator      38..85
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     1933..1949
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      1950..1981
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             100..3691
                     /translation="MLDIVELSRLQFALTAMYHFLFVPLTLGMAFLLAIMETVYVLSGKQIYKDMTKFWGKLFGINFALGVATGLTMEFQFGTNWSYYSHYVGDIFGAPLAIEGLMAFFLESTFVGLFFFGWDRLGKVQHMCVTWLVALGSNLSALWILVANGWMQNPIASDFNFETMRMEMVSFSELVLNPVAQVKFVHTVASGYVTGAMFILGISAWYMLKGRDFAFAKRSFAIAASFGMAAVLSVIVLGDESGYEMGDVQKTKLAAIEAEWETQPAPAAFTLFGIPDQEEETNKFAIQIPYALGIIATRSVDTPVIGLKELMVQHEERIRNGMKAYSLLEQLRSGSTDQAVRDQFNSMKKDLGYGLLLKRYTPNVADATEAQIQQATKDSIPRVAPLYFAFRIMVACGFLLLAIIALSFWSVIRNRIGEKKWLLRAALYGIPLPWIAVEAGWFVAEYGRQPWAIGEVLPTAVANSSLTAGDLIFSMVLICGLYTLFLVAELFLMFKFARLGPSSLKTGRYHFEQSSTTTQPAR"
                     /label="cydA"
                     /note="A terminal oxidase that produces a proton motive force by the vectorial transfer of protons across the inner membrane. It is the component of the aerobic respiratory chain of E.coli that predominates when cells are grown at low aeration. Generates a proton motive force using protons and electrons from opposite sides of the membrane to generate H(2)O, transferring 1 proton/electron."
     primer_bind     complement(90..106)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(173..761)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(952..1812)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(1813..1917)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
     misc_feature    3633..3692
                     /label="FreeGenes Barcode"
                     /note="FreeGenes Barcode. Defined as I-SceI site flanked by 21 base pair random sequences."
ORIGIN
        1 CGCTGAGGTG TCAATCGTCG GAGCCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC
       61 TAAACGGGTC TTGAGGGGTT TTTTGCATGG TCATAGCTGT TTCCTGAGAG CTTGGCAGGT
      121 GATGACACAC ATTAACAAAT TTCGTGAGGA GTCTCCAGAA GAATGCCATT AATTTCCATA
      181 GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC
      241 CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG
      301 TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC
      361 TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG
      421 GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC
      481 TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA
      541 TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG
      601 GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA
      661 AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG
      721 TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGGCCTA CTATTAGCAA
      781 CAACGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA
      841 AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA
      901 ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAAACT TGGTCTGACA GTTACCAATG
      961 CTTAATCAGT GAGGCACCTA TCTCAGCGAT CTGTCTATTT CGTTCATCCA TAGTTGCCTG
     1021 ACTCCCCGTC GTGTAGATAA CTACGATACG GGAGGGCTTA CCATCTGGCC CCAGTGCTGC
     1081 AATGATACCG CGAGAACCAC GCTCACCGGC TCCAGATTTA TCAGCAATAA ACCAGCCAGC
     1141 CGGAAGGGCC GAGCGCAGAA GTGGTCCTGC AACTTTATCC GCCTCCATCC AGTCTATTAA
     1201 TTGTTGCCGG GAAGCTAGAG TAAGTAGTTC GCCAGTTAAT AGTTTGCGCA ACGTTGTTGC
     1261 CATTGCTACA GGCATCGTGG TGTCACGCTC GTCGTTTGGT ATGGCTTCAT TCAGCTCCGG
     1321 TTCCCAACGA TCAAGGCGAG TTACATGATC CCCCATGTTG TGCAAAAAAG CGGTTAGCTC
     1381 CTTCGGTCCT CCGATCGTTG TCAGAAGTAA GTTGGCCGCA GTGTTATCAC TCATGGTTAT
     1441 GGCAGCACTG CATAATTCTC TTACTGTCAT GCCATCCGTA AGATGCTTTT CTGTGACTGG
     1501 TGAGTACTCA ACCAAGTCAT TCTGAGAATA GTGTATGCGG CGACCGAGTT GCTCTTGCCC
     1561 GGCGTCAATA CGGGATAATA CCGCGCCACA TAGCAGAACT TTAAAAGTGC TCATCATTGG
     1621 AAAACGTTCT TCGGGGCGAA AACTCTCAAG GATCTTACCG CTGTTGAGAT CCAGTTCGAT
     1681 GTAACCCACT CGTGCACCCA ACTGATCTTC AGCATCTTTT ACTTTCACCA GCGTTTCTGG
     1741 GTGAGCAAAA ACAGGAAGGC AAAATGCCGC AAAAAAGGGA ATAAGGGCGA CACGGAAATG
     1801 TTGAATACTC ATACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT
     1861 CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC
     1921 CTGCACCAGT CAGTAAAACG ACGGCCAGTA GTCAAAAGCC TCCGACCGGA GGCTTTTGAC
     1981 TTGGTTCAGG TGGAGTGGGA GAAAGCACTC TTAGGCCTCT GGGTCTCTAA TGCTAGACAT
     2041 CGTCGAACTA TCACGTCTAC AGTTCGCACT AACCGCAATG TACCACTTCC TATTCGTCCC
     2101 GCTAACCCTA GGAATGGCAT TCCTACTAGC AATCATGGAA ACCGTCTACG TCCTATCAGG
     2161 AAAACAGATC TACAAAGACA TGACCAAATT CTGGGGAAAA CTATTCGGAA TCAACTTCGC
     2221 ACTAGGAGTC GCAACCGGAC TAACCATGGA ATTCCAGTTC GGAACCAACT GGTCATACTA
     2281 CTCACACTAC GTCGGAGACA TCTTCGGAGC ACCGCTAGCA ATCGAAGGAC TAATGGCATT
     2341 CTTCCTAGAA TCAACCTTCG TCGGACTATT CTTCTTCGGA TGGGACCGTC TAGGAAAAGT
     2401 CCAGCACATG TGCGTCACCT GGCTAGTCGC ACTAGGATCA AACCTATCAG CACTATGGAT
     2461 CCTAGTCGCA AACGGATGGA TGCAGAACCC GATCGCATCA GACTTCAACT TCGAAACCAT
     2521 GCGTATGGAA ATGGTCTCAT TCTCAGAACT AGTCCTAAAC CCGGTCGCAC AGGTCAAATT
     2581 CGTCCACACC GTCGCATCAG GATACGTCAC CGGAGCAATG TTCATCCTAG GAATCTCAGC
     2641 ATGGTACATG CTAAAAGGAC GTGACTTCGC ATTCGCAAAA CGTTCATTCG CAATCGCAGC
     2701 ATCATTCGGA ATGGCAGCAG TCCTATCAGT CATCGTCCTA GGAGACGAAT CAGGATACGA
     2761 AATGGGAGAC GTCCAGAAAA CCAAACTAGC AGCAATCGAA GCAGAATGGG AAACCCAGCC
     2821 GGCACCGGCA GCATTCACCC TATTCGGAAT CCCGGACCAG GAAGAAGAAA CCAACAAATT
     2881 CGCAATCCAG ATCCCGTACG CACTAGGAAT CATCGCAACC CGTTCAGTCG ACACCCCGGT
     2941 CATCGGACTA AAAGAACTAA TGGTCCAGCA CGAAGAACGT ATCCGTAACG GAATGAAAGC
     3001 ATACTCACTA CTAGAACAGC TACGTTCAGG ATCAACCGAC CAGGCAGTCC GTGACCAGTT
     3061 CAACTCAATG AAAAAAGACC TAGGATACGG ACTACTACTA AAACGTTACA CCCCGAACGT
     3121 CGCAGACGCA ACCGAAGCAC AGATCCAGCA GGCAACCAAA GACTCAATCC CGCGTGTCGC
     3181 ACCGCTATAC TTCGCATTCC GTATCATGGT CGCATGCGGA TTCCTACTAC TAGCAATCAT
     3241 CGCACTATCA TTCTGGTCAG TCATCCGTAA CCGTATCGGA GAAAAAAAAT GGCTACTACG
     3301 TGCAGCACTA TACGGAATCC CGCTACCGTG GATCGCAGTC GAAGCAGGAT GGTTCGTCGC
     3361 AGAATACGGA CGTCAGCCGT GGGCAATCGG AGAAGTCCTA CCGACCGCAG TCGCAAACTC
     3421 ATCACTAACC GCAGGAGACC TAATCTTCTC AATGGTCCTA ATCTGCGGAC TATACACCCT
     3481 ATTCCTAGTC GCAGAACTAT TCCTAATGTT CAAATTCGCA CGTCTAGGAC CGTCATCACT
     3541 AAAAACCGGA CGTTACCACT TCGAACAGTC ATCAACCACC ACCCAGCCGG CACGTTGAAG
     3601 AGCTTCGAGA CCAGCTATGA TCCCGGTGTA ACGCCCGACG TGAGAAGTCC AGGTAGGGAT
     3661 AACAGGGTAA TTAGTTTTAA GAATGCCTAC GA
//