LOCUS       Exported     3872 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..3872
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      1909..1956
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     3804..3820
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      3821..3852
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             100..3871
                     /translation="MHNDKDLSTWQTFRRLWPTIAPFKAGLIVAGVALILNAASDTFMLSLLKPLLDDGFGKTDRSVLVWMPLVVIGLMILRGITSYVSSYCISWVSGKVVMTMRRRLFGHMMGMPVSFFDKQSTGTLLSRITYDSEQVASSSSGALITVVREGASIIGLFIMMFYYSWQLSIILIVLAPIVSIAIRVVSKRFRNISKNMQNTMGQVTTSAEQMLKGHKEVLIFGGQEVETKRFDKVSNRMRLQGMKMVSASSISDPIIQLIASLALAFVLYAASFPSVMDSLTAGTITVVFSSMIALMRPLKSLTNVNAQFQRGMAACQTLFTILDSEQEKDEGKRVIERATGDVEFRNVTFTYPGRDVPALRNINLKIPAGKTVALVGRSGSGKSTIASLITRFYDIDEGEILMDGHDLREYTLASLRNQVALVSQNVHLFNDTVANNIAYARTEQYSREQIEEAARMAYAMDFINKMDNGLDTVIGENGVLLSGGQRQRIAIARALLRDSPILILDEATSALDTESERAIQAALDELQKNRTSLVIAHRLSTIEKADEIVVVEDGVIVERGTHNDLLEHRGVYAQLHKMQFGQ"
                     /label="msbA"
                     /note="Involved in lipid A export and possibly also in glycerophospholipid export and for biogenesis of the outer membrane. Transmembrane domains (TMD) form a pore in the inner membrane and the ATP-binding domain (NBD) is responsible for energy generation."
     primer_bind     complement(1961..1977)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(2044..2632)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(2823..3683)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(3684..3788)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
     misc_feature    1812..1871
                     /label="FreeGenes Barcode"
                     /note="FreeGenes Barcode. Defined as I-SceI site flanked by 21 base pair random sequences."
ORIGIN
        1 AAACACGTGG CAAACATTCC GGTCTCTAAT GCACAACGAC AAAGACCTAT CAACCTGGCA
       61 GACCTTCCGT CGTCTATGGC CGACCATCGC ACCGTTCAAA GCAGGACTAA TCGTCGCAGG
      121 AGTCGCACTA ATCCTAAACG CAGCATCAGA CACCTTCATG CTATCACTAC TAAAACCGCT
      181 ACTAGACGAC GGATTCGGAA AAACCGACCG TTCAGTCCTA GTCTGGATGC CGCTAGTCGT
      241 CATCGGACTA ATGATCCTAC GTGGAATCAC CTCATACGTC TCATCATACT GCATCTCATG
      301 GGTCTCAGGA AAAGTCGTCA TGACCATGCG TCGTCGTCTA TTCGGACACA TGATGGGAAT
      361 GCCGGTCTCA TTCTTCGACA AACAGTCAAC CGGAACCCTA CTATCACGTA TCACCTACGA
      421 CTCAGAACAG GTCGCATCAT CATCATCAGG AGCACTAATC ACCGTCGTCC GTGAAGGAGC
      481 ATCAATCATC GGACTATTCA TCATGATGTT CTACTACTCA TGGCAGCTAT CAATCATCCT
      541 AATCGTCCTA GCACCGATCG TCTCAATCGC AATCCGTGTC GTCTCAAAAC GTTTCCGTAA
      601 CATCTCAAAA AACATGCAGA ACACCATGGG ACAGGTCACC ACCTCAGCAG AACAGATGCT
      661 AAAAGGACAC AAAGAAGTCC TAATCTTCGG AGGACAGGAA GTCGAAACCA AACGTTTCGA
      721 CAAAGTCTCA AACCGTATGC GTCTACAGGG AATGAAAATG GTCTCAGCAT CATCAATCTC
      781 AGACCCGATC ATCCAGCTAA TCGCATCACT AGCACTAGCA TTCGTCCTAT ACGCAGCATC
      841 ATTCCCGTCA GTCATGGACT CACTAACCGC AGGAACCATC ACCGTCGTCT TCTCATCAAT
      901 GATCGCACTA ATGCGTCCGC TAAAATCACT AACCAACGTC AACGCACAGT TCCAGCGTGG
      961 AATGGCAGCA TGCCAGACCC TATTCACCAT CCTAGACTCA GAACAGGAAA AAGACGAAGG
     1021 AAAACGTGTC ATCGAACGTG CAACCGGAGA CGTCGAATTC CGTAACGTCA CCTTCACCTA
     1081 CCCGGGACGT GACGTCCCGG CACTACGTAA CATCAACCTA AAAATCCCGG CAGGAAAAAC
     1141 CGTCGCACTA GTCGGACGTT CAGGATCAGG AAAATCAACC ATCGCATCAC TAATCACCCG
     1201 TTTCTACGAC ATCGACGAAG GAGAAATCCT AATGGACGGA CACGACCTAC GTGAATACAC
     1261 CCTAGCATCA CTACGTAACC AGGTCGCACT AGTCTCACAG AACGTCCACC TATTCAACGA
     1321 CACCGTCGCA AACAACATCG CATACGCACG TACCGAACAG TACTCACGTG AACAGATCGA
     1381 AGAAGCAGCA CGTATGGCAT ACGCAATGGA CTTCATCAAC AAAATGGACA ACGGACTAGA
     1441 CACCGTCATC GGAGAAAACG GAGTCCTACT ATCAGGAGGA CAGCGTCAGC GTATCGCAAT
     1501 CGCACGTGCA CTACTACGTG ACTCACCGAT CCTAATCCTA GACGAAGCAA CCTCAGCACT
     1561 AGACACCGAA TCAGAACGTG CAATCCAGGC AGCACTAGAC GAACTACAGA AAAACCGTAC
     1621 CTCACTAGTC ATCGCACACC GTCTATCAAC CATCGAAAAA GCAGACGAAA TCGTCGTCGT
     1681 CGAAGACGGA GTCATCGTCG AACGTGGAAC CCACAACGAC CTACTAGAAC ACCGTGGAGT
     1741 CTACGCACAG CTACACAAAA TGCAGTTCGG ACAGTGAAGA GCTTCGAGAC CAGATAGATG
     1801 CTCCGTCAAG CGGTAGTCTG CAAGACGCCG TGTAGGGATA ACAGGGTAAT TTCGCTCGCT
     1861 TCAACGCAGC TCGCTGAGGT GTCAATCGTC GGAGCCGCTG AGCAATAACT AGCATAACCC
     1921 CTTGGGGCCT CTAAACGGGT CTTGAGGGGT TTTTTGCATG GTCATAGCTG TTTCCTGAGA
     1981 GCTTGGCAGG TGATGACACA CATTAACAAA TTTCGTGAGG AGTCTCCAGA AGAATGCCAT
     2041 TAATTTCCAT AGGCTCCGCC CCCCTGACGA GCATCACAAA AATCGACGCT CAAGTCAGAG
     2101 GTGGCGAAAC CCGACAGGAC TATAAAGATA CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT
     2161 GCGCTCTCCT GTTCCGACCC TGCCGCTTAC CGGATACCTG TCCGCCTTTC TCCCTTCGGG
     2221 AAGCGTGGCG CTTTCTCATA GCTCACGCTG TAGGTATCTC AGTTCGGTGT AGGTCGTTCG
     2281 CTCCAAGCTG GGCTGTGTGC ACGAACCCCC CGTTCAGCCC GACCGCTGCG CCTTATCCGG
     2341 TAACTATCGT CTTGAGTCCA ACCCGGTAAG ACACGACTTA TCGCCACTGG CAGCAGCCAC
     2401 TGGTAACAGG ATTAGCAGAG CGAGGTATGT AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG
     2461 GCCTAACTAC GGCTACACTA GAAGAACAGT ATTTGGTATC TGCGCTCTGC TGAAGCCAGT
     2521 TACCTTCGGA AAAAGAGTTG GTAGCTCTTG ATCCGGCAAA CAAACCACCG CTGGTAGCGG
     2581 TGGTTTTTTT GTTTGCAAGC AGCAGATTAC GCGCAGAAAA AAAGGATCTC AAGAAGGCCT
     2641 ACTATTAGCA ACAACGATCC TTTGATCTTT TCTACGGGGT CTGACGCTCA GTGGAACGAA
     2701 AACTCACGTT AAGGGATTTT GGTCATGAGA TTATCAAAAA GGATCTTCAC CTAGATCCTT
     2761 TTAAATTAAA AATGAAGTTT TAAATCAATC TAAAGTATAT ATGAGTAAAC TTGGTCTGAC
     2821 AGTTACCAAT GCTTAATCAG TGAGGCACCT ATCTCAGCGA TCTGTCTATT TCGTTCATCC
     2881 ATAGTTGCCT GACTCCCCGT CGTGTAGATA ACTACGATAC GGGAGGGCTT ACCATCTGGC
     2941 CCCAGTGCTG CAATGATACC GCGAGAACCA CGCTCACCGG CTCCAGATTT ATCAGCAATA
     3001 AACCAGCCAG CCGGAAGGGC CGAGCGCAGA AGTGGTCCTG CAACTTTATC CGCCTCCATC
     3061 CAGTCTATTA ATTGTTGCCG GGAAGCTAGA GTAAGTAGTT CGCCAGTTAA TAGTTTGCGC
     3121 AACGTTGTTG CCATTGCTAC AGGCATCGTG GTGTCACGCT CGTCGTTTGG TATGGCTTCA
     3181 TTCAGCTCCG GTTCCCAACG ATCAAGGCGA GTTACATGAT CCCCCATGTT GTGCAAAAAA
     3241 GCGGTTAGCT CCTTCGGTCC TCCGATCGTT GTCAGAAGTA AGTTGGCCGC AGTGTTATCA
     3301 CTCATGGTTA TGGCAGCACT GCATAATTCT CTTACTGTCA TGCCATCCGT AAGATGCTTT
     3361 TCTGTGACTG GTGAGTACTC AACCAAGTCA TTCTGAGAAT AGTGTATGCG GCGACCGAGT
     3421 TGCTCTTGCC CGGCGTCAAT ACGGGATAAT ACCGCGCCAC ATAGCAGAAC TTTAAAAGTG
     3481 CTCATCATTG GAAAACGTTC TTCGGGGCGA AAACTCTCAA GGATCTTACC GCTGTTGAGA
     3541 TCCAGTTCGA TGTAACCCAC TCGTGCACCC AACTGATCTT CAGCATCTTT TACTTTCACC
     3601 AGCGTTTCTG GGTGAGCAAA AACAGGAAGG CAAAATGCCG CAAAAAAGGG AATAAGGGCG
     3661 ACACGGAAAT GTTGAATACT CATACTCTTC CTTTTTCAAT ATTATTGAAG CATTTATCAG
     3721 GGTTATTGTC TCATGAGCGG ATACATATTT GAATGTATTT AGAAAAATAA ACAAATAGGG
     3781 GTTCCGCGCA CCTGCACCAG TCAGTAAAAC GACGGCCAGT AGTCAAAAGC CTCCGACCGG
     3841 AGGCTTTTGA CTTGGTTCAG GTGGAGTGGG AG
//