LOCUS Exported 3446 bp DNA circular SYN 24-JUL-2020 DEFINITION synthetic linear DNA ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2001) AUTHORS Keoni Gandall TITLE Direct Submission JOURNAL Exported from Poly. FEATURES Location/Qualifiers terminator 1..3446 /note="transcription terminator for bacteriophage T7 RNA polymerase" /label="T7 terminator" terminator 38..85 /label="T7 terminator" /note="transcription terminator for bacteriophage T7 RNA polymerase" primer_bind 1933..1949 /label="M13 for" /note="common sequencing primer, one of multiple similar variants" terminator 1950..1981 /label="tonB terminator" /note="bidirectional E. coli tonB-P14 transcription terminator" CDS 2030..3352 /label="mukF" /note="Involved in chromosome condensation, segregation and cell cycle progression. May participate in facilitating chromosome segregation by condensation DNA from both sides of a centrally located replisome during cell division. Not required for mini-F plasmid partitioning. Probably acts via its interaction with MukB and MukE. Overexpression results in anucleate cells. It has a calcium binding activity." /translation="MSEFSQTVPELVAWARKNDFSISLPVDRLSFLLAVATLNGERLDGEMSEGELVDAFRHVSDAFEQTSETIGVRANNAINDMVRQRLLNRFTSEQAEGNAIYRLTPLGIGITDYYIRQREFSTLRLSMQLSIVAGELKRAADAAEEGGDEFHWHRNVYAPLKYSVAEIFDSIDLTQRLMDEQQQQVKDDIAQLLNKDWRAAISSCELLLSETSGTLRELQDTLEAAGDKLQANLLRIQDATMTHDDLHFVDRLVFDLQSKLDRIISWGQQSIDLWIGYDRHVHKFIRTAIDMDKNRVFAQRLRQSVQTYFDEPWALTYANADRLLDMRDEEMALRDEEVTGELPEDLEYEEFNEIREQLAAIIEEQLAVYKTRQVPLDLGLVVREYLSQYPRARHFDVARIVIDQAVRLGVAQADFTGLPAKWQPINDYGAKVQAHVIDKY" primer_bind complement(90..106) /label="M13 rev" /note="common sequencing primer, one of multiple similar variants" rep_origin complement(173..761) /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(952..1812) /label="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*" promoter complement(1813..1917) /label="bla promoter" /note="ampicillin resistance gene promoter" misc_feature 3387..3446 /label="FreeGenes Barcode" /note="FreeGenes Barcode. Defined as I-SceI site flanked by 21 base pair random sequences." ORIGIN 1 CGCTGAGGTG TCAATCGTCG GAGCCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC 61 TAAACGGGTC TTGAGGGGTT TTTTGCATGG TCATAGCTGT TTCCTGAGAG CTTGGCAGGT 121 GATGACACAC ATTAACAAAT TTCGTGAGGA GTCTCCAGAA GAATGCCATT AATTTCCATA 181 GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC 241 CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG 301 TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC 361 TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG 421 GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC 481 TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA 541 TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG 601 GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA 661 AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG 721 TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGGCCTA CTATTAGCAA 781 CAACGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA 841 AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA 901 ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAAACT TGGTCTGACA GTTACCAATG 961 CTTAATCAGT GAGGCACCTA TCTCAGCGAT CTGTCTATTT CGTTCATCCA TAGTTGCCTG 1021 ACTCCCCGTC GTGTAGATAA CTACGATACG GGAGGGCTTA CCATCTGGCC CCAGTGCTGC 1081 AATGATACCG CGAGAACCAC GCTCACCGGC TCCAGATTTA TCAGCAATAA ACCAGCCAGC 1141 CGGAAGGGCC GAGCGCAGAA GTGGTCCTGC AACTTTATCC GCCTCCATCC AGTCTATTAA 1201 TTGTTGCCGG GAAGCTAGAG TAAGTAGTTC GCCAGTTAAT AGTTTGCGCA ACGTTGTTGC 1261 CATTGCTACA GGCATCGTGG TGTCACGCTC GTCGTTTGGT ATGGCTTCAT TCAGCTCCGG 1321 TTCCCAACGA TCAAGGCGAG TTACATGATC CCCCATGTTG TGCAAAAAAG CGGTTAGCTC 1381 CTTCGGTCCT CCGATCGTTG TCAGAAGTAA GTTGGCCGCA GTGTTATCAC TCATGGTTAT 1441 GGCAGCACTG CATAATTCTC TTACTGTCAT GCCATCCGTA AGATGCTTTT CTGTGACTGG 1501 TGAGTACTCA ACCAAGTCAT TCTGAGAATA GTGTATGCGG CGACCGAGTT GCTCTTGCCC 1561 GGCGTCAATA CGGGATAATA CCGCGCCACA TAGCAGAACT TTAAAAGTGC TCATCATTGG 1621 AAAACGTTCT TCGGGGCGAA AACTCTCAAG GATCTTACCG CTGTTGAGAT CCAGTTCGAT 1681 GTAACCCACT CGTGCACCCA ACTGATCTTC AGCATCTTTT ACTTTCACCA GCGTTTCTGG 1741 GTGAGCAAAA ACAGGAAGGC AAAATGCCGC AAAAAAGGGA ATAAGGGCGA CACGGAAATG 1801 TTGAATACTC ATACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT 1861 CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC 1921 CTGCACCAGT CAGTAAAACG ACGGCCAGTA GTCAAAAGCC TCCGACCGGA GGCTTTTGAC 1981 TTGGTTCAGG TGGAGTGGGA GAAACCGGAG CCATACAGTA CGGTCTCTAA TGTCAGAATT 2041 CTCACAGACC GTCCCGGAAC TAGTCGCATG GGCACGTAAA AACGACTTCT CAATCTCACT 2101 ACCGGTCGAC CGTCTATCAT TCCTACTAGC AGTCGCAACC CTAAACGGAG AACGTCTAGA 2161 CGGAGAAATG TCAGAAGGAG AACTAGTCGA CGCATTCCGT CACGTCTCAG ACGCATTCGA 2221 ACAGACCTCA GAAACCATCG GAGTCCGTGC AAACAACGCA ATCAACGACA TGGTCCGTCA 2281 GCGTCTACTA AACCGTTTCA CCTCAGAACA GGCAGAAGGA AACGCAATCT ACCGTCTAAC 2341 CCCGCTAGGA ATCGGAATCA CCGACTACTA CATCCGTCAG CGTGAATTCT CAACCCTACG 2401 TCTATCAATG CAGCTATCAA TCGTCGCAGG AGAACTAAAA CGTGCAGCAG ACGCAGCAGA 2461 AGAAGGAGGA GACGAATTCC ACTGGCACCG TAACGTCTAC GCACCGCTAA AATACTCAGT 2521 CGCAGAAATC TTCGACTCAA TCGACCTAAC CCAGCGTCTA ATGGACGAAC AGCAGCAGCA 2581 GGTCAAAGAC GACATCGCAC AGCTACTAAA CAAAGACTGG CGTGCAGCAA TCTCATCATG 2641 CGAACTACTA CTATCAGAAA CCTCAGGAAC CCTACGTGAA CTACAGGACA CCCTAGAAGC 2701 AGCAGGAGAC AAACTACAGG CAAACCTACT ACGTATCCAG GACGCAACCA TGACCCACGA 2761 CGACCTACAC TTCGTCGACC GTCTAGTCTT CGACCTACAG TCAAAACTAG ACCGTATCAT 2821 CTCATGGGGA CAGCAGTCAA TCGACCTATG GATCGGATAC GACCGTCACG TCCACAAATT 2881 CATCCGTACC GCAATCGACA TGGACAAAAA CCGTGTCTTC GCACAGCGTC TACGTCAGTC 2941 AGTCCAGACC TACTTCGACG AACCGTGGGC ACTAACCTAC GCAAACGCAG ACCGTCTACT 3001 AGACATGCGT GACGAAGAAA TGGCACTACG TGACGAAGAA GTCACCGGAG AACTACCGGA 3061 AGACCTAGAA TACGAAGAAT TCAACGAAAT CCGTGAACAG CTAGCAGCAA TCATCGAAGA 3121 ACAGCTAGCA GTCTACAAAA CCCGTCAGGT CCCGCTAGAC CTAGGACTAG TCGTCCGTGA 3181 ATACCTATCA CAGTACCCGC GTGCACGTCA CTTCGACGTC GCACGTATCG TCATCGACCA 3241 GGCAGTCCGT CTAGGAGTCG CACAGGCAGA CTTCACCGGA CTACCGGCAA AATGGCAGCC 3301 GATCAACGAC TACGGAGCAA AAGTCCAGGC ACACGTCATC GACAAATACT GAAGAGCTTC 3361 GAGACCAGAC AAGCCTTAAC CGTAGGCAGA TCCTGACTCC CGACTCCTAG GGATAACAGG 3421 GTAATAAAGT TCTCGGCGTC GAACTA //