LOCUS Exported 3494 bp DNA circular SYN 24-JUL-2020 DEFINITION synthetic linear DNA ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2001) AUTHORS Keoni Gandall TITLE Direct Submission JOURNAL Exported from Poly. FEATURES Location/Qualifiers terminator 1..3494 /label="T7 terminator" /note="transcription terminator for bacteriophage T7 RNA polymerase" terminator 1531..1578 /label="T7 terminator" /note="transcription terminator for bacteriophage T7 RNA polymerase" primer_bind 3426..3442 /label="M13 for" /note="common sequencing primer, one of multiple similar variants" terminator 3443..3474 /label="tonB terminator" /note="bidirectional E. coli tonB-P14 transcription terminator" CDS 100..3493 /label="purB" /note="" /translation="MELSSLTAVSPVDGRYGDKVSALRGIFSEYGLLKFRVQVEVRWLQKLAAHAAIKEVPAFAADAIGYLDAIVASFSEEDAARIKTIERTTNHDVKAVEYFLKEKVAEIPELHAVSEFIHFACTSEDINNLSHALMLKTARDEVILPYWRQLIDGIKDLAVQYRDIPLLSRTHGQPATPSTIGKEMANVAYRMERQYRQLNQVEILGKINGAVGNYNAHIAAYPEVDWHQFSEEFVTSLGIQWNPYTTQIEPHDYIAELFDCVARFNTILIDFDRDVWGYIALNHFKQKTIAGEIGSSTMPHKVNPIDFENSEGNLGLSNAVLQHLASKLPVSRWQRDLTDSTVLRNLGVGIGYALIAYQSTLKGVSKLEVNRDHLLDELDHNWEVLAEPIQTVMRRYGIEKPYEKLKELTRGKRVDAEGMKQFIDGLALPEEEKARLKAMTPANYIGRAITMVDELK" primer_bind complement(1583..1599) /label="M13 rev" /note="common sequencing primer, one of multiple similar variants" rep_origin complement(1666..2254) /label="ori" /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(2445..3305) /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*" /label="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" promoter complement(3306..3410) /note="ampicillin resistance gene promoter" /label="bla promoter" misc_feature 1434..1493 /note="FreeGenes Barcode. Defined as I-SceI site flanked by 21 base pair random sequences." /label="FreeGenes Barcode" ORIGIN 1 AAACCGGAGC CATACAGTAC GGTCTCTAAT GGAACTATCA TCACTAACCG CAGTCTCACC 61 GGTCGACGGA CGTTACGGAG ACAAAGTCTC AGCACTACGT GGAATCTTCT CAGAATACGG 121 ACTACTAAAA TTCCGTGTCC AGGTCGAAGT CCGTTGGCTA CAGAAACTAG CAGCACACGC 181 AGCAATCAAA GAAGTCCCGG CATTCGCAGC AGACGCAATC GGATACCTAG ACGCAATCGT 241 CGCATCATTC TCAGAAGAAG ACGCAGCACG TATCAAAACC ATCGAACGTA CCACCAACCA 301 CGACGTCAAA GCAGTCGAAT ACTTCCTAAA AGAAAAAGTC GCAGAAATCC CGGAACTACA 361 CGCAGTCTCA GAATTCATCC ACTTCGCATG CACCTCAGAA GACATCAACA ACCTATCACA 421 CGCACTAATG CTAAAAACCG CACGTGACGA AGTCATCCTA CCGTACTGGC GTCAGCTAAT 481 CGACGGAATC AAAGACCTAG CAGTCCAGTA CCGTGACATC CCGCTACTAT CACGTACCCA 541 CGGACAGCCG GCAACCCCGT CAACCATCGG AAAAGAAATG GCAAACGTCG CATACCGTAT 601 GGAACGTCAG TACCGTCAGC TAAACCAGGT CGAAATCCTA GGAAAAATCA ACGGAGCAGT 661 CGGAAACTAC AACGCACACA TCGCAGCATA CCCGGAAGTC GACTGGCACC AGTTCTCAGA 721 AGAATTCGTC ACCTCACTAG GAATCCAGTG GAACCCGTAC ACCACCCAGA TCGAACCGCA 781 CGACTACATC GCAGAACTAT TCGACTGCGT CGCACGTTTC AACACCATCC TAATCGACTT 841 CGACCGTGAC GTCTGGGGAT ACATCGCACT AAACCACTTC AAACAGAAAA CCATCGCAGG 901 AGAAATCGGA TCATCAACCA TGCCGCACAA AGTCAACCCG ATCGACTTCG AAAACTCAGA 961 AGGAAACCTA GGACTATCAA ACGCAGTCCT ACAGCACCTA GCATCAAAAC TACCGGTCTC 1021 ACGTTGGCAG CGTGACCTAA CCGACTCAAC CGTCCTACGT AACCTAGGAG TCGGAATCGG 1081 ATACGCACTA ATCGCATACC AGTCAACCCT AAAAGGAGTC TCAAAACTAG AAGTCAACCG 1141 TGACCACCTA CTAGACGAAC TAGACCACAA CTGGGAAGTC CTAGCAGAAC CGATCCAGAC 1201 CGTCATGCGT CGTTACGGAA TCGAAAAACC GTACGAAAAA CTAAAAGAAC TAACCCGTGG 1261 AAAACGTGTC GACGCAGAAG GAATGAAACA GTTCATCGAC GGACTAGCAC TACCGGAAGA 1321 AGAAAAAGCA CGTCTAAAAG CAATGACCCC GGCAAACTAC ATCGGACGTG CAATCACCAT 1381 GGTCGACGAA CTAAAATGAA GAGCTTCGAG ACCAATGCGT CATTTTACAC GGCAGTTCAC 1441 ACTCGAGTGT GATCTAGGGA TAACAGGGTA ATTGATATTG AAGATCCTGA TAGCGCTGAG 1501 GTGTCAATCG TCGGAGCCGC TGAGCAATAA CTAGCATAAC CCCTTGGGGC CTCTAAACGG 1561 GTCTTGAGGG GTTTTTTGCA TGGTCATAGC TGTTTCCTGA GAGCTTGGCA GGTGATGACA 1621 CACATTAACA AATTTCGTGA GGAGTCTCCA GAAGAATGCC ATTAATTTCC ATAGGCTCCG 1681 CCCCCCTGAC GAGCATCACA AAAATCGACG CTCAAGTCAG AGGTGGCGAA ACCCGACAGG 1741 ACTATAAAGA TACCAGGCGT TTCCCCCTGG AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC 1801 CCTGCCGCTT ACCGGATACC TGTCCGCCTT TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA 1861 TAGCTCACGC TGTAGGTATC TCAGTTCGGT GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT 1921 GCACGAACCC CCCGTTCAGC CCGACCGCTG CGCCTTATCC GGTAACTATC GTCTTGAGTC 1981 CAACCCGGTA AGACACGACT TATCGCCACT GGCAGCAGCC ACTGGTAACA GGATTAGCAG 2041 AGCGAGGTAT GTAGGCGGTG CTACAGAGTT CTTGAAGTGG TGGCCTAACT ACGGCTACAC 2101 TAGAAGAACA GTATTTGGTA TCTGCGCTCT GCTGAAGCCA GTTACCTTCG GAAAAAGAGT 2161 TGGTAGCTCT TGATCCGGCA AACAAACCAC CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA 2221 GCAGCAGATT ACGCGCAGAA AAAAAGGATC TCAAGAAGGC CTACTATTAG CAACAACGAT 2281 CCTTTGATCT TTTCTACGGG GTCTGACGCT CAGTGGAACG AAAACTCACG TTAAGGGATT 2341 TTGGTCATGA GATTATCAAA AAGGATCTTC ACCTAGATCC TTTTAAATTA AAAATGAAGT 2401 TTTAAATCAA TCTAAAGTAT ATATGAGTAA ACTTGGTCTG ACAGTTACCA ATGCTTAATC 2461 AGTGAGGCAC CTATCTCAGC GATCTGTCTA TTTCGTTCAT CCATAGTTGC CTGACTCCCC 2521 GTCGTGTAGA TAACTACGAT ACGGGAGGGC TTACCATCTG GCCCCAGTGC TGCAATGATA 2581 CCGCGAGAAC CACGCTCACC GGCTCCAGAT TTATCAGCAA TAAACCAGCC AGCCGGAAGG 2641 GCCGAGCGCA GAAGTGGTCC TGCAACTTTA TCCGCCTCCA TCCAGTCTAT TAATTGTTGC 2701 CGGGAAGCTA GAGTAAGTAG TTCGCCAGTT AATAGTTTGC GCAACGTTGT TGCCATTGCT 2761 ACAGGCATCG TGGTGTCACG CTCGTCGTTT GGTATGGCTT CATTCAGCTC CGGTTCCCAA 2821 CGATCAAGGC GAGTTACATG ATCCCCCATG TTGTGCAAAA AAGCGGTTAG CTCCTTCGGT 2881 CCTCCGATCG TTGTCAGAAG TAAGTTGGCC GCAGTGTTAT CACTCATGGT TATGGCAGCA 2941 CTGCATAATT CTCTTACTGT CATGCCATCC GTAAGATGCT TTTCTGTGAC TGGTGAGTAC 3001 TCAACCAAGT CATTCTGAGA ATAGTGTATG CGGCGACCGA GTTGCTCTTG CCCGGCGTCA 3061 ATACGGGATA ATACCGCGCC ACATAGCAGA ACTTTAAAAG TGCTCATCAT TGGAAAACGT 3121 TCTTCGGGGC GAAAACTCTC AAGGATCTTA CCGCTGTTGA GATCCAGTTC GATGTAACCC 3181 ACTCGTGCAC CCAACTGATC TTCAGCATCT TTTACTTTCA CCAGCGTTTC TGGGTGAGCA 3241 AAAACAGGAA GGCAAAATGC CGCAAAAAAG GGAATAAGGG CGACACGGAA ATGTTGAATA 3301 CTCATACTCT TCCTTTTTCA ATATTATTGA AGCATTTATC AGGGTTATTG TCTCATGAGC 3361 GGATACATAT TTGAATGTAT TTAGAAAAAT AAACAAATAG GGGTTCCGCG CACCTGCACC 3421 AGTCAGTAAA ACGACGGCCA GTAGTCAAAA GCCTCCGACC GGAGGCTTTT GACTTGGTTC 3481 AGGTGGAGTG GGAG //