LOCUS       Exported     3494 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..3494
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      1531..1578
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     3426..3442
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      3443..3474
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             100..3493
                     /label="purB"
                     /note=""
                     /translation="MELSSLTAVSPVDGRYGDKVSALRGIFSEYGLLKFRVQVEVRWLQKLAAHAAIKEVPAFAADAIGYLDAIVASFSEEDAARIKTIERTTNHDVKAVEYFLKEKVAEIPELHAVSEFIHFACTSEDINNLSHALMLKTARDEVILPYWRQLIDGIKDLAVQYRDIPLLSRTHGQPATPSTIGKEMANVAYRMERQYRQLNQVEILGKINGAVGNYNAHIAAYPEVDWHQFSEEFVTSLGIQWNPYTTQIEPHDYIAELFDCVARFNTILIDFDRDVWGYIALNHFKQKTIAGEIGSSTMPHKVNPIDFENSEGNLGLSNAVLQHLASKLPVSRWQRDLTDSTVLRNLGVGIGYALIAYQSTLKGVSKLEVNRDHLLDELDHNWEVLAEPIQTVMRRYGIEKPYEKLKELTRGKRVDAEGMKQFIDGLALPEEEKARLKAMTPANYIGRAITMVDELK"
     primer_bind     complement(1583..1599)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(1666..2254)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(2445..3305)
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
     promoter        complement(3306..3410)
                     /note="ampicillin resistance gene promoter"
                     /label="bla promoter"
     misc_feature    1434..1493
                     /note="FreeGenes Barcode. Defined as I-SceI site flanked by 21 base pair random sequences."
                     /label="FreeGenes Barcode"
ORIGIN
        1 AAACCGGAGC CATACAGTAC GGTCTCTAAT GGAACTATCA TCACTAACCG CAGTCTCACC
       61 GGTCGACGGA CGTTACGGAG ACAAAGTCTC AGCACTACGT GGAATCTTCT CAGAATACGG
      121 ACTACTAAAA TTCCGTGTCC AGGTCGAAGT CCGTTGGCTA CAGAAACTAG CAGCACACGC
      181 AGCAATCAAA GAAGTCCCGG CATTCGCAGC AGACGCAATC GGATACCTAG ACGCAATCGT
      241 CGCATCATTC TCAGAAGAAG ACGCAGCACG TATCAAAACC ATCGAACGTA CCACCAACCA
      301 CGACGTCAAA GCAGTCGAAT ACTTCCTAAA AGAAAAAGTC GCAGAAATCC CGGAACTACA
      361 CGCAGTCTCA GAATTCATCC ACTTCGCATG CACCTCAGAA GACATCAACA ACCTATCACA
      421 CGCACTAATG CTAAAAACCG CACGTGACGA AGTCATCCTA CCGTACTGGC GTCAGCTAAT
      481 CGACGGAATC AAAGACCTAG CAGTCCAGTA CCGTGACATC CCGCTACTAT CACGTACCCA
      541 CGGACAGCCG GCAACCCCGT CAACCATCGG AAAAGAAATG GCAAACGTCG CATACCGTAT
      601 GGAACGTCAG TACCGTCAGC TAAACCAGGT CGAAATCCTA GGAAAAATCA ACGGAGCAGT
      661 CGGAAACTAC AACGCACACA TCGCAGCATA CCCGGAAGTC GACTGGCACC AGTTCTCAGA
      721 AGAATTCGTC ACCTCACTAG GAATCCAGTG GAACCCGTAC ACCACCCAGA TCGAACCGCA
      781 CGACTACATC GCAGAACTAT TCGACTGCGT CGCACGTTTC AACACCATCC TAATCGACTT
      841 CGACCGTGAC GTCTGGGGAT ACATCGCACT AAACCACTTC AAACAGAAAA CCATCGCAGG
      901 AGAAATCGGA TCATCAACCA TGCCGCACAA AGTCAACCCG ATCGACTTCG AAAACTCAGA
      961 AGGAAACCTA GGACTATCAA ACGCAGTCCT ACAGCACCTA GCATCAAAAC TACCGGTCTC
     1021 ACGTTGGCAG CGTGACCTAA CCGACTCAAC CGTCCTACGT AACCTAGGAG TCGGAATCGG
     1081 ATACGCACTA ATCGCATACC AGTCAACCCT AAAAGGAGTC TCAAAACTAG AAGTCAACCG
     1141 TGACCACCTA CTAGACGAAC TAGACCACAA CTGGGAAGTC CTAGCAGAAC CGATCCAGAC
     1201 CGTCATGCGT CGTTACGGAA TCGAAAAACC GTACGAAAAA CTAAAAGAAC TAACCCGTGG
     1261 AAAACGTGTC GACGCAGAAG GAATGAAACA GTTCATCGAC GGACTAGCAC TACCGGAAGA
     1321 AGAAAAAGCA CGTCTAAAAG CAATGACCCC GGCAAACTAC ATCGGACGTG CAATCACCAT
     1381 GGTCGACGAA CTAAAATGAA GAGCTTCGAG ACCAATGCGT CATTTTACAC GGCAGTTCAC
     1441 ACTCGAGTGT GATCTAGGGA TAACAGGGTA ATTGATATTG AAGATCCTGA TAGCGCTGAG
     1501 GTGTCAATCG TCGGAGCCGC TGAGCAATAA CTAGCATAAC CCCTTGGGGC CTCTAAACGG
     1561 GTCTTGAGGG GTTTTTTGCA TGGTCATAGC TGTTTCCTGA GAGCTTGGCA GGTGATGACA
     1621 CACATTAACA AATTTCGTGA GGAGTCTCCA GAAGAATGCC ATTAATTTCC ATAGGCTCCG
     1681 CCCCCCTGAC GAGCATCACA AAAATCGACG CTCAAGTCAG AGGTGGCGAA ACCCGACAGG
     1741 ACTATAAAGA TACCAGGCGT TTCCCCCTGG AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC
     1801 CCTGCCGCTT ACCGGATACC TGTCCGCCTT TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA
     1861 TAGCTCACGC TGTAGGTATC TCAGTTCGGT GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT
     1921 GCACGAACCC CCCGTTCAGC CCGACCGCTG CGCCTTATCC GGTAACTATC GTCTTGAGTC
     1981 CAACCCGGTA AGACACGACT TATCGCCACT GGCAGCAGCC ACTGGTAACA GGATTAGCAG
     2041 AGCGAGGTAT GTAGGCGGTG CTACAGAGTT CTTGAAGTGG TGGCCTAACT ACGGCTACAC
     2101 TAGAAGAACA GTATTTGGTA TCTGCGCTCT GCTGAAGCCA GTTACCTTCG GAAAAAGAGT
     2161 TGGTAGCTCT TGATCCGGCA AACAAACCAC CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA
     2221 GCAGCAGATT ACGCGCAGAA AAAAAGGATC TCAAGAAGGC CTACTATTAG CAACAACGAT
     2281 CCTTTGATCT TTTCTACGGG GTCTGACGCT CAGTGGAACG AAAACTCACG TTAAGGGATT
     2341 TTGGTCATGA GATTATCAAA AAGGATCTTC ACCTAGATCC TTTTAAATTA AAAATGAAGT
     2401 TTTAAATCAA TCTAAAGTAT ATATGAGTAA ACTTGGTCTG ACAGTTACCA ATGCTTAATC
     2461 AGTGAGGCAC CTATCTCAGC GATCTGTCTA TTTCGTTCAT CCATAGTTGC CTGACTCCCC
     2521 GTCGTGTAGA TAACTACGAT ACGGGAGGGC TTACCATCTG GCCCCAGTGC TGCAATGATA
     2581 CCGCGAGAAC CACGCTCACC GGCTCCAGAT TTATCAGCAA TAAACCAGCC AGCCGGAAGG
     2641 GCCGAGCGCA GAAGTGGTCC TGCAACTTTA TCCGCCTCCA TCCAGTCTAT TAATTGTTGC
     2701 CGGGAAGCTA GAGTAAGTAG TTCGCCAGTT AATAGTTTGC GCAACGTTGT TGCCATTGCT
     2761 ACAGGCATCG TGGTGTCACG CTCGTCGTTT GGTATGGCTT CATTCAGCTC CGGTTCCCAA
     2821 CGATCAAGGC GAGTTACATG ATCCCCCATG TTGTGCAAAA AAGCGGTTAG CTCCTTCGGT
     2881 CCTCCGATCG TTGTCAGAAG TAAGTTGGCC GCAGTGTTAT CACTCATGGT TATGGCAGCA
     2941 CTGCATAATT CTCTTACTGT CATGCCATCC GTAAGATGCT TTTCTGTGAC TGGTGAGTAC
     3001 TCAACCAAGT CATTCTGAGA ATAGTGTATG CGGCGACCGA GTTGCTCTTG CCCGGCGTCA
     3061 ATACGGGATA ATACCGCGCC ACATAGCAGA ACTTTAAAAG TGCTCATCAT TGGAAAACGT
     3121 TCTTCGGGGC GAAAACTCTC AAGGATCTTA CCGCTGTTGA GATCCAGTTC GATGTAACCC
     3181 ACTCGTGCAC CCAACTGATC TTCAGCATCT TTTACTTTCA CCAGCGTTTC TGGGTGAGCA
     3241 AAAACAGGAA GGCAAAATGC CGCAAAAAAG GGAATAAGGG CGACACGGAA ATGTTGAATA
     3301 CTCATACTCT TCCTTTTTCA ATATTATTGA AGCATTTATC AGGGTTATTG TCTCATGAGC
     3361 GGATACATAT TTGAATGTAT TTAGAAAAAT AAACAAATAG GGGTTCCGCG CACCTGCACC
     3421 AGTCAGTAAA ACGACGGCCA GTAGTCAAAA GCCTCCGACC GGAGGCTTTT GACTTGGTTC
     3481 AGGTGGAGTG GGAG
//