LOCUS       Exported     3944 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..3944
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      1981..2028
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
                     /label="T7 terminator"
     primer_bind     3876..3892
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      3893..3924
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
                     /label="tonB terminator"
     primer_bind     1..20
                     /label="dialout_for"
                     /note="Reverse dialout primer. Unique pair for each FreeGenes part."
     primer_bind     1864..1883
                     /label="dialout_rev"
                     /note="Forward dialout primer. Unique pair for each FreeGenes part."
     CDS             29..1849
                     /translation="MVISYDNYVTILDEETLKAWIAKLEKAPVFAFDTETDSLDNISANLVGLSFAIEPGVAAYIPVAHDYLDAPDQISRERALELLKPLLEDEKALKVGQNLKYDRGILANYGIELRGIAFDTMLESYILNSVAGRHDMDSLAERWLKHKTITFEEIAGKGKNQLTFNQIALEEAGRYAAEDADVTLQLHLKMWPDLQKHKGPLNVFENIEMPLVPVLSRIERNGVKIDPKVLHNHSEELTLRLAELEKKAHEIAGEEFNLSSTKQLQTILFEKQGIKPLKKTPGGAPSTSEEVLEELALDYPLPKVILEYRGLAKLKSTYTDKLPLMINPKTGRVHTSYHQAVTATGRLSSTDPNLQNIPVRNEEGRRIRQAFIAPEDYVIVSADYSQIELRIMAHLSRDKGLLTAFAEGKDIHRATAAEVFGLPLETVTSEQRRSAKAINFGLIYGMSAFGLARQLNIPRKEAQKYMDLYFERYPGVLEYMERTRAQAKEQGYVETLDGRRLYLPDIKSSNGARRAAAERAAINAPMQGTAADIIKRAMIAVDAWLQAEQPRVRMIMQVHDELVFEVHKDDVDAVAKQIHQLMENCTRLDVPLLVEVGSGENWDQAH*"
                     /label="DNA Polymerase I, Large (Klenow) Fragment"
                     /note=""
     primer_bind     complement(2033..2049)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(2116..2704)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(2895..3755)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(3756..3860)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
     misc_feature    1884..1943
                     /label="FreeGenes Barcode"
                     /note="FreeGenes Barcode. Defined as I-SceI site flanked by 21 base pair random sequences."
ORIGIN
        1 AAAGGGGCCG TCAATATCAG GGTCTCTAAT GGTGATTTCC TATGATAACT ACGTTACCAT
       61 CCTGGACGAA GAAACGCTGA AAGCCTGGAT CGCAAAACTG GAAAAAGCGC CGGTTTTCGC
      121 CTTCGACACC GAAACCGATA GCCTGGATAA CATTAGCGCT AACTTGGTTG GCCTGTCCTT
      181 TGCTATTGAG CCGGGTGTTG CTGCGTACAT CCCTGTGGCG CACGACTACC TGGACGCTCC
      241 AGACCAGATT TCTCGTGAAC GTGCTCTGGA ACTGCTGAAA CCACTGCTGG AGGACGAAAA
      301 AGCGCTGAAA GTAGGTCAAA ATCTGAAATA TGACCGTGGT ATTCTGGCGA ACTACGGCAT
      361 CGAATTGCGT GGTATTGCTT TTGACACGAT GCTGGAATCT TACATTCTGA ACTCCGTGGC
      421 AGGCCGCCAC GATATGGACA GCCTGGCCGA ACGATGGCTG AAACACAAAA CCATCACTTT
      481 TGAAGAAATC GCGGGTAAAG GCAAAAACCA GCTGACTTTC AACCAGATCG CTTTAGAAGA
      541 AGCAGGTCGT TATGCTGCTG AGGACGCGGA TGTGACCCTG CAACTCCACT TGAAAATGTG
      601 GCCTGACCTG CAAAAACATA AAGGTCCACT GAACGTATTC GAGAACATCG AGATGCCGCT
      661 GGTACCGGTT CTGTCTCGTA TTGAACGTAA TGGCGTCAAA ATCGATCCGA AGGTGCTGCA
      721 TAACCACAGT GAAGAACTGA CGCTGCGTCT GGCTGAATTA GAAAAGAAAG CGCATGAAAT
      781 TGCTGGTGAA GAGTTCAACT TAAGCTCGAC CAAGCAGCTG CAAACCATTT TATTCGAAAA
      841 ACAGGGCATC AAACCGCTGA AAAAGACACC GGGGGGTGCG CCGTCTACCT CTGAAGAAGT
      901 GCTGGAAGAA CTGGCGCTGG ACTACCCGCT GCCTAAAGTT ATCTTGGAAT ACCGTGGTCT
      961 GGCCAAACTG AAAAGCACCT ATACTGACAA ACTGCCGCTT ATGATTAATC CGAAAACGGG
     1021 CCGTGTTCAC ACCAGCTACC ACCAGGCTGT GACCGCAACT GGTCGCTTAT CTAGCACTGA
     1081 TCCGAATCTG CAAAATATCC CGGTGCGCAA CGAAGAAGGC CGTCGTATCC GCCAGGCGTT
     1141 TATTGCACCG GAAGATTATG TCATTGTTTC CGCAGACTAC TCTCAAATCG AGCTGCGTAT
     1201 CATGGCTCAC CTGTCTCGTG ATAAAGGTTT ACTCACCGCA TTCGCTGAAG GCAAAGACAT
     1261 TCATCGTGCA ACCGCAGCGG AAGTGTTCGG CCTGCCGCTG GAAACCGTTA CCAGCGAACA
     1321 GCGCCGTTCC GCAAAGGCAA TTAACTTTGG TCTGATTTAC GGCATGTCTG CTTTTGGCCT
     1381 GGCGCGACAG CTGAACATTC CGCGTAAGGA AGCACAGAAG TACATGGACC TGTATTTCGA
     1441 ACGTTACCCG GGCGTTCTGG AGTACATGGA ACGTACCCGT GCACAGGCCA AAGAACAGGG
     1501 CTATGTTGAA ACTCTGGATG GTCGCCGCCT GTATTTGCCG GACATCAAGT CTAGCAACGG
     1561 CGCTCGCCGC GCAGCCGCGG AGCGTGCTGC GATCAACGCT CCGATGCAGG GTACCGCGGC
     1621 GGATATTATC AAACGCGCCA TGATCGCAGT CGACGCGTGG CTGCAAGCAG AACAGCCGCG
     1681 TGTTCGTATG ATTATGCAGG TTCACGACGA ACTGGTGTTC GAAGTGCATA AAGACGATGT
     1741 AGATGCAGTC GCAAAGCAGA TCCATCAGCT GATGGAAAAC TGCACCCGTC TGGACGTTCC
     1801 GCTCCTGGTT GAAGTTGGTA GTGGTGAAAA CTGGGACCAG GCACATTGAA GAGCTTTGAG
     1861 ACCACAGGAA GCAAGGTATA CGCAGGCATG CACCGTGTCC TATGTAGGGA TAACAGGGTA
     1921 ATAGACCACG AGGGCCACCC ATCCGCTGAG GTGTCAATCG TCGGAGCCGC TGAGCAATAA
     1981 CTAGCATAAC CCCTTGGGGC CTCTAAACGG GTCTTGAGGG GTTTTTTGCA TGGTCATAGC
     2041 TGTTTCCTGA GAGCTTGGCA GGTGATGACA CACATTAACA AATTTCGTGA GGAGTCTCCA
     2101 GAAGAATGCC ATTAATTTCC ATAGGCTCCG CCCCCCTGAC GAGCATCACA AAAATCGACG
     2161 CTCAAGTCAG AGGTGGCGAA ACCCGACAGG ACTATAAAGA TACCAGGCGT TTCCCCCTGG
     2221 AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC CCTGCCGCTT ACCGGATACC TGTCCGCCTT
     2281 TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA TAGCTCACGC TGTAGGTATC TCAGTTCGGT
     2341 GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT GCACGAACCC CCCGTTCAGC CCGACCGCTG
     2401 CGCCTTATCC GGTAACTATC GTCTTGAGTC CAACCCGGTA AGACACGACT TATCGCCACT
     2461 GGCAGCAGCC ACTGGTAACA GGATTAGCAG AGCGAGGTAT GTAGGCGGTG CTACAGAGTT
     2521 CTTGAAGTGG TGGCCTAACT ACGGCTACAC TAGAAGAACA GTATTTGGTA TCTGCGCTCT
     2581 GCTGAAGCCA GTTACCTTCG GAAAAAGAGT TGGTAGCTCT TGATCCGGCA AACAAACCAC
     2641 CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA GCAGCAGATT ACGCGCAGAA AAAAAGGATC
     2701 TCAAGAAGGC CTACTATTAG CAACAACGAT CCTTTGATCT TTTCTACGGG GTCTGACGCT
     2761 CAGTGGAACG AAAACTCACG TTAAGGGATT TTGGTCATGA GATTATCAAA AAGGATCTTC
     2821 ACCTAGATCC TTTTAAATTA AAAATGAAGT TTTAAATCAA TCTAAAGTAT ATATGAGTAA
     2881 ACTTGGTCTG ACAGTTACCA ATGCTTAATC AGTGAGGCAC CTATCTCAGC GATCTGTCTA
     2941 TTTCGTTCAT CCATAGTTGC CTGACTCCCC GTCGTGTAGA TAACTACGAT ACGGGAGGGC
     3001 TTACCATCTG GCCCCAGTGC TGCAATGATA CCGCGAGAAC CACGCTCACC GGCTCCAGAT
     3061 TTATCAGCAA TAAACCAGCC AGCCGGAAGG GCCGAGCGCA GAAGTGGTCC TGCAACTTTA
     3121 TCCGCCTCCA TCCAGTCTAT TAATTGTTGC CGGGAAGCTA GAGTAAGTAG TTCGCCAGTT
     3181 AATAGTTTGC GCAACGTTGT TGCCATTGCT ACAGGCATCG TGGTGTCACG CTCGTCGTTT
     3241 GGTATGGCTT CATTCAGCTC CGGTTCCCAA CGATCAAGGC GAGTTACATG ATCCCCCATG
     3301 TTGTGCAAAA AAGCGGTTAG CTCCTTCGGT CCTCCGATCG TTGTCAGAAG TAAGTTGGCC
     3361 GCAGTGTTAT CACTCATGGT TATGGCAGCA CTGCATAATT CTCTTACTGT CATGCCATCC
     3421 GTAAGATGCT TTTCTGTGAC TGGTGAGTAC TCAACCAAGT CATTCTGAGA ATAGTGTATG
     3481 CGGCGACCGA GTTGCTCTTG CCCGGCGTCA ATACGGGATA ATACCGCGCC ACATAGCAGA
     3541 ACTTTAAAAG TGCTCATCAT TGGAAAACGT TCTTCGGGGC GAAAACTCTC AAGGATCTTA
     3601 CCGCTGTTGA GATCCAGTTC GATGTAACCC ACTCGTGCAC CCAACTGATC TTCAGCATCT
     3661 TTTACTTTCA CCAGCGTTTC TGGGTGAGCA AAAACAGGAA GGCAAAATGC CGCAAAAAAG
     3721 GGAATAAGGG CGACACGGAA ATGTTGAATA CTCATACTCT TCCTTTTTCA ATATTATTGA
     3781 AGCATTTATC AGGGTTATTG TCTCATGAGC GGATACATAT TTGAATGTAT TTAGAAAAAT
     3841 AAACAAATAG GGGTTCCGCG CACCTGCACC AGTCAGTAAA ACGACGGCCA GTAGTCAAAA
     3901 GCCTCCGACC GGAGGCTTTT GACTTGGTTC AGGTGGAGTG GGAG
//