LOCUS       Exported     3353 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..3353
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      1390..1437
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     3285..3301
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      3302..3333
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     primer_bind     1..20
                     /label="dialout_for"
                     /note="Reverse dialout primer. Unique pair for each FreeGenes part."
     primer_bind     1273..1292
                     /label="dialout_rev"
                     /note="Forward dialout primer. Unique pair for each FreeGenes part."
     CDS             29..1258
                     /label="DNA Polymerase I, Large (Klenow) Fragment (3′→5′ exo-)"
                     /note=""
                     /translation="MGPLNVFENIEMPLVPVLSRIERNGVKIDPKVLHNHSEELTLRLAELEKKAHEIAGEEFNLSSTKQLQTILFEKQGIKPLKKTPGGAPSTSEEVLEELALDYPLPKVILEYRGLAKLRSTYTDKLPLMINPKTGRVHTSYHQAVTATGRLSSTDPNLQNIPVRNEEGRRIRQAFIAPEDYVIVSADYSQIELRIMAHLSRDKGLLTAFAEGKDIHRATAAEVFGLPLETVTSEQRRSAKAINFGLIYGMSAFGLARQLNIPRKEAQKYMDLYFERYPGVLEYMERTRAQAKEQGYVETLDGRRLYLPDIKSSNGARRAAAERAAINAPMQGTAADIIKRAMIAVDAWLQAEQPRVRMIMQVHDELVFEVHKDDVDAVAKQIHQLMENCTRLDVPLLVEVGSGENWDQAH*"
     primer_bind     complement(1442..1458)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(1525..2113)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(2304..3164)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(3165..3269)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
     misc_feature    1293..1352
                     /label="FreeGenes Barcode"
                     /note="FreeGenes Barcode. Defined as I-SceI site flanked by 21 base pair random sequences."
ORIGIN
        1 AAAGGGGCCG TCAATATCAG GGTCTCTAAT GGGCCCGTTG AACGTTTTTG AAAACATCGA
       61 GATGCCGCTT GTGCCGGTTC TGTCTCGCAT TGAACGTAAC GGTGTAAAAA TCGACCCGAA
      121 GGTATTGCAC AACCACTCGG AGGAACTCAC CCTTCGTCTG GCTGAACTGG AAAAAAAAGC
      181 TCACGAGATC GCGGGTGAAG AGTTCAACCT GTCGAGCACT AAGCAGCTGC AAACCATCCT
      241 GTTCGAGAAA CAAGGTATTA AACCGCTGAA AAAAACTCCA GGTGGTGCTC CGAGCACTAG
      301 CGAAGAAGTG CTGGAAGAAC TGGCCCTCGA TTATCCGTTG CCAAAAGTTA TTCTGGAGTA
      361 CCGCGGCCTC GCCAAACTGA GGAGCACCTA CACCGACAAA CTGCCTCTGA TGATTAACCC
      421 GAAAACGGGC CGTGTTCATA CCTCCTACCA CCAGGCCGTT ACCGCTACTG GCCGTCTGAG
      481 CTCTACCGAC CCGAATCTGC AAAACATCCC TGTACGTAAT GAAGAAGGTC GTCGTATCCG
      541 TCAGGCGTTC ATTGCTCCGG AGGACTATGT AATTGTTTCT GCGGATTATT CCCAGATCGA
      601 ACTGCGTATC ATGGCACACC TGTCTCGTGA TAAAGGCCTT TTAACCGCCT TCGCTGAAGG
      661 TAAAGATATC CACCGTGCAA CTGCGGCTGA AGTTTTCGGC CTTCCGCTGG AAACCGTTAC
      721 CTCGGAACAG CGCCGTTCTG CTAAAGCCAT CAACTTTGGC TTGATCTACG GCATGAGTGC
      781 GTTCGGTCTG GCACGTCAGC TGAACATCCC GCGTAAAGAA GCTCAAAAAT ATATGGACCT
      841 GTACTTTGAG CGTTATCCGG GTGTTCTGGA GTACATGGAG CGCACCCGTG CTCAGGCGAA
      901 AGAACAGGGC TATGTGGAAA CTCTGGACGG CCGTCGTCTG TACCTGCCGG ACATCAAATC
      961 TTCAAACGGT GCACGTCGTG CAGCTGCGGA ACGCGCCGCT ATCAACGCTC CGATGCAGGG
     1021 TACTGCGGCG GATATTATTA AACGCGCTAT GATCGCAGTT GATGCTTGGC TGCAAGCTGA
     1081 ACAGCCGCGC GTTCGTATGA TCATGCAGGT GCATGATGAA CTGGTTTTCG AGGTGCACAA
     1141 GGACGATGTA GACGCAGTGG CTAAACAGAT TCACCAGCTT ATGGAAAACT GCACGCGTCT
     1201 GGACGTTCCG CTTCTGGTGG AAGTGGGCTC TGGCGAAAAC TGGGATCAGG CTCATTGAAG
     1261 AGCTTTGAGA CCACAGGGTA TATTGAGTGC CCTGGCAAGG TCGAAATTCT ACGTAGGGAT
     1321 AACAGGGTAA TGCAACGGGG CAGACTCCGA TACGCTGAGG TGTCAATCGT CGGAGCCGCT
     1381 GAGCAATAAC TAGCATAACC CCTTGGGGCC TCTAAACGGG TCTTGAGGGG TTTTTTGCAT
     1441 GGTCATAGCT GTTTCCTGAG AGCTTGGCAG GTGATGACAC ACATTAACAA ATTTCGTGAG
     1501 GAGTCTCCAG AAGAATGCCA TTAATTTCCA TAGGCTCCGC CCCCCTGACG AGCATCACAA
     1561 AAATCGACGC TCAAGTCAGA GGTGGCGAAA CCCGACAGGA CTATAAAGAT ACCAGGCGTT
     1621 TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC TGTTCCGACC CTGCCGCTTA CCGGATACCT
     1681 GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC GCTTTCTCAT AGCTCACGCT GTAGGTATCT
     1741 CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT GGGCTGTGTG CACGAACCCC CCGTTCAGCC
     1801 CGACCGCTGC GCCTTATCCG GTAACTATCG TCTTGAGTCC AACCCGGTAA GACACGACTT
     1861 ATCGCCACTG GCAGCAGCCA CTGGTAACAG GATTAGCAGA GCGAGGTATG TAGGCGGTGC
     1921 TACAGAGTTC TTGAAGTGGT GGCCTAACTA CGGCTACACT AGAAGAACAG TATTTGGTAT
     1981 CTGCGCTCTG CTGAAGCCAG TTACCTTCGG AAAAAGAGTT GGTAGCTCTT GATCCGGCAA
     2041 ACAAACCACC GCTGGTAGCG GTGGTTTTTT TGTTTGCAAG CAGCAGATTA CGCGCAGAAA
     2101 AAAAGGATCT CAAGAAGGCC TACTATTAGC AACAACGATC CTTTGATCTT TTCTACGGGG
     2161 TCTGACGCTC AGTGGAACGA AAACTCACGT TAAGGGATTT TGGTCATGAG ATTATCAAAA
     2221 AGGATCTTCA CCTAGATCCT TTTAAATTAA AAATGAAGTT TTAAATCAAT CTAAAGTATA
     2281 TATGAGTAAA CTTGGTCTGA CAGTTACCAA TGCTTAATCA GTGAGGCACC TATCTCAGCG
     2341 ATCTGTCTAT TTCGTTCATC CATAGTTGCC TGACTCCCCG TCGTGTAGAT AACTACGATA
     2401 CGGGAGGGCT TACCATCTGG CCCCAGTGCT GCAATGATAC CGCGAGAACC ACGCTCACCG
     2461 GCTCCAGATT TATCAGCAAT AAACCAGCCA GCCGGAAGGG CCGAGCGCAG AAGTGGTCCT
     2521 GCAACTTTAT CCGCCTCCAT CCAGTCTATT AATTGTTGCC GGGAAGCTAG AGTAAGTAGT
     2581 TCGCCAGTTA ATAGTTTGCG CAACGTTGTT GCCATTGCTA CAGGCATCGT GGTGTCACGC
     2641 TCGTCGTTTG GTATGGCTTC ATTCAGCTCC GGTTCCCAAC GATCAAGGCG AGTTACATGA
     2701 TCCCCCATGT TGTGCAAAAA AGCGGTTAGC TCCTTCGGTC CTCCGATCGT TGTCAGAAGT
     2761 AAGTTGGCCG CAGTGTTATC ACTCATGGTT ATGGCAGCAC TGCATAATTC TCTTACTGTC
     2821 ATGCCATCCG TAAGATGCTT TTCTGTGACT GGTGAGTACT CAACCAAGTC ATTCTGAGAA
     2881 TAGTGTATGC GGCGACCGAG TTGCTCTTGC CCGGCGTCAA TACGGGATAA TACCGCGCCA
     2941 CATAGCAGAA CTTTAAAAGT GCTCATCATT GGAAAACGTT CTTCGGGGCG AAAACTCTCA
     3001 AGGATCTTAC CGCTGTTGAG ATCCAGTTCG ATGTAACCCA CTCGTGCACC CAACTGATCT
     3061 TCAGCATCTT TTACTTTCAC CAGCGTTTCT GGGTGAGCAA AAACAGGAAG GCAAAATGCC
     3121 GCAAAAAAGG GAATAAGGGC GACACGGAAA TGTTGAATAC TCATACTCTT CCTTTTTCAA
     3181 TATTATTGAA GCATTTATCA GGGTTATTGT CTCATGAGCG GATACATATT TGAATGTATT
     3241 TAGAAAAATA AACAAATAGG GGTTCCGCGC ACCTGCACCA GTCAGTAAAA CGACGGCCAG
     3301 TAGTCAAAAG CCTCCGACCG GAGGCTTTTG ACTTGGTTCA GGTGGAGTGG GAG
//