LOCUS       Exported     4910 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..4910
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      2947..2994
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     4842..4858
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      4859..4890
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     primer_bind     1..20
                     /label="dialout_for"
                     /note="Reverse dialout primer. Unique pair for each FreeGenes part."
     primer_bind     2830..2849
                     /label="dialout_rev"
                     /note="Forward dialout primer. Unique pair for each FreeGenes part."
     CDS             29..2815
                     /label="E. coli DNA Polymerase I"
                     /note="In addition to polymerase activity, this DNA polymerase exhibits 3'-5' and 5'-3' exonuclease activity. It is able to utilize nicked circular duplex DNA as a template and can unwind the parental DNA strand from its template."
                     /translation="MVQIPQNPLILVDGSSYLYRAYHAFPPLTNSAGEPTGAMYGVLNMLRSLIMQYKPTHAAVVFDAKGKTFRDELFEHYKSHRPPMPDDLRAQIEPLHAMVKAMGLPLLAVSGVEADDVIGTLAREAEKAGRPVLISTGDKDMAQLVTPNITLINTMTNTILGPEEVVNKYGVPPELIIDFLALMGDSSDNIPGVPGVGEKTAQALLQGLGGLDTLYAEPEKIAGLSFRGAKTMAAKLEQNKEVAYLSYQLATIKTDVELELTCEQLEVQQPAAEELLGLFKKYEFKRWTADVEAGKWLQAKGAKPAAKPQETSVADEAPEVTATVISYDNYVTILDEETLKAWIAKLEKAPVFAFDTETDSLDNISANLVGLSFAIEPGVAAYIPVAHDYLDAPDQISRERALELLKPLLEDEKALKVGQNLKYDRGILANYGIELRGIAFDTMLESYILNSVAGRHDMDSLAERWLKHKTITFEEIAGKGKNQLTFNQIALEEAGRYAAEDADVTLQLHLKMWPDLQKHKGPLNVFENIEMPLVPVLSRIERNGVKIDPKVLHNHSEELTLRLAELEKKAHEIAGEEFNLSSTKQLQTILFEKQGIKPLKKTPGGAPSTSEEVLEELALDYPLPKVILEYRGLAKLKSTYTDKLPLMINPKTGRVHTSYHQAVTATGRLSSTDPNLQNIPVRNEEGRRIRQAFIAPEDYVIVSADYSQIELRIMAHLSRDKGLLTAFAEGKDIHRATAAEVFGLPLETVTSEQRRSAKAINFGLIYGMSAFGLARQLNIPRKEAQKYMDLYFERYPGVLEYMERTRAQAKEQGYVETLDGRRLYLPDIKSSNGARRAAAERAAINAPMQGTAADIIKRAMIAVDAWLQAEQPRVRMIMQVHDELVFEVHKDDVDAVAKQIHQLMENCTRLDVPLLVEVGSGENWDQAH*"
     primer_bind     complement(2999..3015)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(3082..3670)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(3861..4721)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(4722..4826)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
     misc_feature    2850..2909
                     /label="FreeGenes Barcode"
                     /note="FreeGenes Barcode. Defined as I-SceI site flanked by 21 base pair random sequences."
ORIGIN
        1 AAAGGGGCCG TCAATATCAG GGTCTCTAAT GGTGCAGATC CCGCAAAACC CGCTGATCTT
       61 GGTGGATGGC TCATCCTACC TGTACCGTGC TTATCACGCG TTCCCGCCAC TGACAAACTC
      121 TGCGGGTGAA CCGACCGGCG CTATGTACGG TGTGCTGAAC ATGCTGCGCT CCCTGATCAT
      181 GCAGTACAAA CCGACACATG CGGCTGTGGT TTTCGACGCC AAGGGTAAAA CCTTTCGTGA
      241 CGAACTGTTT GAACATTACA AATCCCACCG TCCGCCGATG CCGGATGATC TGCGCGCTCA
      301 GATCGAACCG CTGCACGCCA TGGTTAAAGC TATGGGCCTG CCGCTGCTGG CTGTTTCCGG
      361 TGTAGAGGCG GACGATGTGA TCGGCACCCT GGCCCGCGAA GCGGAGAAGG CTGGTCGTCC
      421 GGTACTGATC TCCACCGGTG ATAAAGATAT GGCGCAGCTG GTCACCCCGA ACATCACCTT
      481 AATCAACACC ATGACAAACA CGATCTTGGG GCCGGAAGAA GTTGTGAATA AATATGGCGT
      541 ACCTCCGGAA CTGATCATCG ACTTTCTGGC CCTTATGGGT GACTCCTCCG ACAACATTCC
      601 GGGTGTACCT GGCGTGGGGG AAAAGACTGC ACAGGCTTTG CTGCAAGGCC TGGGTGGCCT
      661 GGACACGTTG TATGCGGAAC CGGAAAAAAT CGCGGGCCTG AGCTTCCGTG GCGCGAAAAC
      721 TATGGCGGCT AAACTTGAAC AGAACAAAGA AGTCGCTTAT CTGTCTTACC AGCTGGCTAC
      781 CATCAAAACC GACGTGGAAC TGGAGCTTAC TTGCGAACAG CTTGAAGTGC AGCAGCCGGC
      841 AGCGGAAGAA CTGCTGGGAC TGTTCAAAAA ATACGAATTT AAACGCTGGA CCGCGGACGT
      901 GGAGGCGGGT AAATGGCTGC AAGCTAAAGG TGCAAAGCCG GCCGCGAAAC CGCAGGAAAC
      961 GTCTGTAGCT GATGAGGCGC CGGAAGTGAC CGCCACCGTT ATCTCCTACG ATAATTACGT
     1021 CACCATCCTG GACGAGGAAA CGCTGAAAGC ATGGATTGCT AAACTGGAAA AAGCGCCGGT
     1081 TTTTGCGTTC GATACCGAAA CTGATTCTCT GGACAACATC AGCGCAAACC TGGTCGGCTT
     1141 ATCTTTTGCT ATTGAACCGG GTGTGGCTGC GTATATTCCG GTGGCCCACG ATTATCTGGA
     1201 TGCCCCGGAT CAGATTTCCC GTGAACGTGC TCTGGAACTG CTGAAACCGC TGCTGGAGGA
     1261 TGAGAAGGCA CTGAAAGTGG GCCAGAACCT GAAATACGAT CGTGGTATCC TGGCGAACTA
     1321 CGGCATCGAG CTGCGTGGCA TTGCCTTTGA TACCATGCTG GAATCCTACA TTCTGAACTC
     1381 CGTGGCAGGT CGTCACGATA TGGATTCTCT GGCGGAACGT TGGCTGAAAC ACAAAACCAT
     1441 CACCTTTGAG GAAATCGCCG GTAAAGGCAA AAACCAGCTC ACCTTCAACC AGATCGCGCT
     1501 GGAAGAGGCG GGTCGCTATG CCGCAGAGGA CGCCGACGTT ACTCTGCAAC TGCACCTGAA
     1561 AATGTGGCCG GACCTTCAGA AACACAAAGG TCCGCTGAAC GTGTTCGAAA ATATCGAAAT
     1621 GCCGCTGGTA CCGGTGTTAT CCCGTATCGA ACGTAATGGT GTTAAAATCG ACCCGAAAGT
     1681 TCTGCATAAC CACTCTGAAG AACTTACTCT GCGTCTGGCG GAGCTGGAAA AGAAGGCTCA
     1741 CGAAATCGCA GGTGAAGAAT TTAACCTTTC TAGCACCAAA CAGCTGCAAA CTATTCTGTT
     1801 CGAAAAACAG GGTATCAAGC CGCTGAAGAA AACCCCGGGT GGTGCGCCGT CCACCTCTGA
     1861 AGAAGTTCTG GAAGAACTGG CACTGGATTA TCCACTGCCG AAAGTTATTC TGGAATACCG
     1921 TGGCCTGGCA AAGCTGAAAT CGACTTACAC GGACAAACTG CCGCTGATGA TCAACCCGAA
     1981 AACCGGCCGT GTTCACACCT CTTATCACCA GGCTGTTACT GCGACTGGCC GTCTGAGCTC
     2041 TACCGACCCG AACCTGCAAA ACATCCCGGT GCGTAATGAA GAAGGTCGTC GTATCCGTCA
     2101 GGCCTTCATC GCACCGGAGG ATTACGTAAT CGTTTCTGCG GACTACTCCC AGATTGAGCT
     2161 GCGCATCATG GCTCATCTGT CCCGTGATAA GGGTCTGCTG ACTGCGTTTG CTGAAGGTAA
     2221 AGACATTCAC CGTGCTACCG CGGCGGAAGT ATTTGGCCTG CCGCTGGAAA CGGTTACTAG
     2281 CGAACAGCGT CGCAGCGCTA AAGCGATCAA CTTCGGTCTG ATCTACGGCA TGTCCGCATT
     2341 TGGCCTGGCA CGTCAACTGA ACATTCCGCG TAAAGAGGCG CAGAAGTACA TGGATCTGTA
     2401 CTTCGAACGT TACCCGGGCG TGCTTGAATA CATGGAGCGT ACCCGTGCCC AAGCCAAAGA
     2461 GCAGGGTTAC GTTGAAACCC TGGATGGCCG TCGTTTATAC CTGCCGGATA TTAAATCCTC
     2521 CAACGGCGCC CGTCGTGCAG CGGCAGAGCG TGCCGCCATC AACGCACCGA TGCAGGGCAC
     2581 CGCAGCGGAC ATTATTAAAC GTGCAATGAT TGCGGTAGAC GCTTGGCTGC AAGCGGAACA
     2641 GCCGCGTGTT CGTATGATCA TGCAAGTGCA TGACGAACTG GTTTTCGAAG TACATAAAGA
     2701 CGATGTCGAT GCTGTAGCCA AACAGATTCA TCAACTGATG GAAAACTGCA CCCGCCTGGA
     2761 CGTGCCTCTG CTGGTTGAAG TGGGCTCCGG CGAAAACTGG GACCAAGCAC ATTGAAGAGC
     2821 TTTGAGACCA CATAAGCGAT CCCAAGGTCC CCAGGTTAAG GCATGCATAT TAGGGATAAC
     2881 AGGGTAATCC AGCACAAACG ATGTTGAATC GCTGAGGTGT CAATCGTCGG AGCCGCTGAG
     2941 CAATAACTAG CATAACCCCT TGGGGCCTCT AAACGGGTCT TGAGGGGTTT TTTGCATGGT
     3001 CATAGCTGTT TCCTGAGAGC TTGGCAGGTG ATGACACACA TTAACAAATT TCGTGAGGAG
     3061 TCTCCAGAAG AATGCCATTA ATTTCCATAG GCTCCGCCCC CCTGACGAGC ATCACAAAAA
     3121 TCGACGCTCA AGTCAGAGGT GGCGAAACCC GACAGGACTA TAAAGATACC AGGCGTTTCC
     3181 CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT TCCGACCCTG CCGCTTACCG GATACCTGTC
     3241 CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT TTCTCATAGC TCACGCTGTA GGTATCTCAG
     3301 TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG CTGTGTGCAC GAACCCCCCG TTCAGCCCGA
     3361 CCGCTGCGCC TTATCCGGTA ACTATCGTCT TGAGTCCAAC CCGGTAAGAC ACGACTTATC
     3421 GCCACTGGCA GCAGCCACTG GTAACAGGAT TAGCAGAGCG AGGTATGTAG GCGGTGCTAC
     3481 AGAGTTCTTG AAGTGGTGGC CTAACTACGG CTACACTAGA AGAACAGTAT TTGGTATCTG
     3541 CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA AAGAGTTGGT AGCTCTTGAT CCGGCAAACA
     3601 AACCACCGCT GGTAGCGGTG GTTTTTTTGT TTGCAAGCAG CAGATTACGC GCAGAAAAAA
     3661 AGGATCTCAA GAAGGCCTAC TATTAGCAAC AACGATCCTT TGATCTTTTC TACGGGGTCT
     3721 GACGCTCAGT GGAACGAAAA CTCACGTTAA GGGATTTTGG TCATGAGATT ATCAAAAAGG
     3781 ATCTTCACCT AGATCCTTTT AAATTAAAAA TGAAGTTTTA AATCAATCTA AAGTATATAT
     3841 GAGTAAACTT GGTCTGACAG TTACCAATGC TTAATCAGTG AGGCACCTAT CTCAGCGATC
     3901 TGTCTATTTC GTTCATCCAT AGTTGCCTGA CTCCCCGTCG TGTAGATAAC TACGATACGG
     3961 GAGGGCTTAC CATCTGGCCC CAGTGCTGCA ATGATACCGC GAGAACCACG CTCACCGGCT
     4021 CCAGATTTAT CAGCAATAAA CCAGCCAGCC GGAAGGGCCG AGCGCAGAAG TGGTCCTGCA
     4081 ACTTTATCCG CCTCCATCCA GTCTATTAAT TGTTGCCGGG AAGCTAGAGT AAGTAGTTCG
     4141 CCAGTTAATA GTTTGCGCAA CGTTGTTGCC ATTGCTACAG GCATCGTGGT GTCACGCTCG
     4201 TCGTTTGGTA TGGCTTCATT CAGCTCCGGT TCCCAACGAT CAAGGCGAGT TACATGATCC
     4261 CCCATGTTGT GCAAAAAAGC GGTTAGCTCC TTCGGTCCTC CGATCGTTGT CAGAAGTAAG
     4321 TTGGCCGCAG TGTTATCACT CATGGTTATG GCAGCACTGC ATAATTCTCT TACTGTCATG
     4381 CCATCCGTAA GATGCTTTTC TGTGACTGGT GAGTACTCAA CCAAGTCATT CTGAGAATAG
     4441 TGTATGCGGC GACCGAGTTG CTCTTGCCCG GCGTCAATAC GGGATAATAC CGCGCCACAT
     4501 AGCAGAACTT TAAAAGTGCT CATCATTGGA AAACGTTCTT CGGGGCGAAA ACTCTCAAGG
     4561 ATCTTACCGC TGTTGAGATC CAGTTCGATG TAACCCACTC GTGCACCCAA CTGATCTTCA
     4621 GCATCTTTTA CTTTCACCAG CGTTTCTGGG TGAGCAAAAA CAGGAAGGCA AAATGCCGCA
     4681 AAAAAGGGAA TAAGGGCGAC ACGGAAATGT TGAATACTCA TACTCTTCCT TTTTCAATAT
     4741 TATTGAAGCA TTTATCAGGG TTATTGTCTC ATGAGCGGAT ACATATTTGA ATGTATTTAG
     4801 AAAAATAAAC AAATAGGGGT TCCGCGCACC TGCACCAGTC AGTAAAACGA CGGCCAGTAG
     4861 TCAAAAGCCT CCGACCGGAG GCTTTTGACT TGGTTCAGGT GGAGTGGGAG
//