LOCUS       Exported     3788 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..3788
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      1825..1872
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
                     /label="T7 terminator"
     primer_bind     3720..3736
                     /note="common sequencing primer, one of multiple similar variants"
                     /label="M13 for"
     terminator      3737..3768
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     primer_bind     1..20
                     /note="Reverse dialout primer. Unique pair for each FreeGenes part."
                     /label="dialout_for"
     primer_bind     1708..1727
                     /note="Forward dialout primer. Unique pair for each FreeGenes part."
                     /label="dialout_rev"
     CDS             29..1693
                     /label="KlenTaq1 polymerase "
                     /note=""
                     /translation="MGLLHEFGLLESPKALEEAPWPPPEGAFVGFVLSRKEPMWADLLALAAARGGRVHRAPEPYKALRDLKEARGLLAKDLSVLALREGLGLPPGDDPMLLAYLLDPSNTTPEGVARRYGGEWTEEAGERAALSERLFANLWGRLEGEERLLWLYREVERPLSAVLAHMEATGVRLDVAYLRALSLEVAEEIARLEAEVFRLAGHPFNLNSRDQLERVLFDELGLPAIGKTEKTGKRSTSAAVLEALREAHPIVEKILQYRELTKLKSTYIDPLPDLIHPRTGRLHTRFNQTATATGRLSSSDPNLQNIPVRTPLGQRIRRAFIAEEGWLLVALDYSQIELRVLAHLSGDENLIRVFQEGRDIHTETASWMFGVPREAVDPLMRRAAKTINFGVLYGMSAHRLSQELAIPYEEAQAFIERYFQSFPKVRAWIEKTLEEGRRRGYVETLFGRRRYVPDLEARVKSVREAAERMAFNMPVQGTAADLMKLAMVKLFPRLEEMGARMLLQVHDELVLEAPKERAEAVARLAKEVMEGVYPLAVPLEVEVGIGEDWLSAKE*"
     primer_bind     complement(1877..1893)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(1960..2548)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(2739..3599)
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
     promoter        complement(3600..3704)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
     misc_feature    1728..1787
                     /note="FreeGenes Barcode. Defined as I-SceI site flanked by 21 base pair random sequences."
                     /label="FreeGenes Barcode"
ORIGIN
        1 AAAGGGGCCG TCAATATCAG GGTCTCTAAT GGGCCTCTTA CACGAATTTG GCCTGCTGGA
       61 ATCGCCGAAG GCCCTGGAAG AAGCGCCGTG GCCGCCGCCC GAGGGTGCGT TTGTAGGCTT
      121 CGTGCTGTCT CGCAAAGAAC CGATGTGGGC AGACCTGCTG GCACTGGCGG CGGCCCGCGG
      181 CGGTCGTGTT CACCGTGCAC CGGAACCATA CAAAGCGCTG CGTGACCTGA AAGAAGCGCG
      241 CGGCCTGCTG GCAAAGGATC TGAGCGTGCT CGCCCTTCGT GAAGGCCTGG GTCTGCCGCC
      301 GGGCGACGAT CCGATGCTCT TAGCGTACTT ACTGGACCCG TCTAACACCA CCCCGGAAGG
      361 TGTCGCGCGT CGTTACGGCG GCGAATGGAC TGAAGAAGCG GGTGAACGTG CAGCTCTGAG
      421 CGAACGTCTG TTCGCAAATC TGTGGGGTCG TTTGGAGGGT GAGGAACGCC TGCTGTGGCT
      481 GTACCGTGAA GTAGAGCGTC CGCTGTCTGC GGTTCTGGCT CACATGGAGG CGACTGGCGT
      541 TCGCCTGGAT GTTGCTTATC TTCGTGCGCT GAGTCTGGAA GTGGCTGAAG AGATCGCACG
      601 TCTGGAGGCA GAAGTCTTTC GTCTTGCAGG TCATCCGTTT AACCTGAACT CCCGCGATCA
      661 GCTGGAACGC GTACTGTTTG ACGAGCTGGG TCTGCCGGCT ATCGGTAAAA CTGAAAAAAC
      721 CGGTAAGCGT TCCACTAGCG CAGCAGTCTT GGAGGCGCTC CGCGAAGCTC ACCCGATTGT
      781 GGAAAAAATT CTGCAATACC GTGAACTCAC TAAACTGAAG TCCACCTATA TTGATCCGCT
      841 GCCGGACCTG ATCCACCCTC GTACTGGTCG TTTACATACC CGTTTTAACC AGACCGCTAC
      901 GGCAACCGGT CGTCTGAGTT CCTCTGACCC GAACCTCCAG AATATCCCGG TTCGTACCCC
      961 GCTGGGTCAG CGTATCCGCC GCGCATTCAT TGCCGAGGAA GGCTGGCTGC TGGTAGCACT
     1021 CGACTATTCC CAGATTGAAC TTCGTGTTCT GGCTCATTTG TCTGGCGACG AAAACCTGAT
     1081 CCGCGTGTTT CAGGAAGGTC GTGACATCCA TACCGAGACT GCTTCGTGGA TGTTCGGAGT
     1141 CCCGCGTGAA GCCGTTGATC CGCTGATGCG CCGCGCGGCC AAGACTATCA ACTTCGGTGT
     1201 TCTGTACGGT ATGTCTGCGC ACCGTCTGTC CCAGGAGCTG GCCATTCCGT ACGAAGAAGC
     1261 ACAAGCGTTC ATCGAGCGTT ATTTTCAATC CTTCCCGAAA GTCCGCGCAT GGATTGAAAA
     1321 AACCCTGGAA GAGGGCCGCC GCCGTGGTTA CGTGGAAACC CTGTTTGGCC GCCGTCGCTA
     1381 CGTGCCGGAC CTGGAGGCCC GCGTTAAATC CGTGCGCGAG GCAGCGGAAC GTATGGCGTT
     1441 CAACATGCCT GTTCAGGGTA CGGCAGCCGA CCTGATGAAA CTGGCCATGG TTAAACTGTT
     1501 CCCGCGTCTG GAAGAAATGG GCGCGCGTAT GCTGCTGCAA GTACACGACG AACTGGTCTT
     1561 GGAAGCGCCA AAGGAACGTG CGGAAGCGGT TGCTCGTCTG GCAAAAGAGG TCATGGAGGG
     1621 GGTGTATCCG CTGGCGGTAC CGCTGGAAGT TGAAGTTGGC ATCGGCGAAG ATTGGCTGTC
     1681 TGCTAAAGAG TGAAGAGCTT TGAGACCACA TTAAATTTCG CCGTGGCCCT GGATGATCGC
     1741 CGCTGGGTTA GGGATAACAG GGTAATGGTT TGTATGGCAA CAGCCTTCGC TGAGGTGTCA
     1801 ATCGTCGGAG CCGCTGAGCA ATAACTAGCA TAACCCCTTG GGGCCTCTAA ACGGGTCTTG
     1861 AGGGGTTTTT TGCATGGTCA TAGCTGTTTC CTGAGAGCTT GGCAGGTGAT GACACACATT
     1921 AACAAATTTC GTGAGGAGTC TCCAGAAGAA TGCCATTAAT TTCCATAGGC TCCGCCCCCC
     1981 TGACGAGCAT CACAAAAATC GACGCTCAAG TCAGAGGTGG CGAAACCCGA CAGGACTATA
     2041 AAGATACCAG GCGTTTCCCC CTGGAAGCTC CCTCGTGCGC TCTCCTGTTC CGACCCTGCC
     2101 GCTTACCGGA TACCTGTCCG CCTTTCTCCC TTCGGGAAGC GTGGCGCTTT CTCATAGCTC
     2161 ACGCTGTAGG TATCTCAGTT CGGTGTAGGT CGTTCGCTCC AAGCTGGGCT GTGTGCACGA
     2221 ACCCCCCGTT CAGCCCGACC GCTGCGCCTT ATCCGGTAAC TATCGTCTTG AGTCCAACCC
     2281 GGTAAGACAC GACTTATCGC CACTGGCAGC AGCCACTGGT AACAGGATTA GCAGAGCGAG
     2341 GTATGTAGGC GGTGCTACAG AGTTCTTGAA GTGGTGGCCT AACTACGGCT ACACTAGAAG
     2401 AACAGTATTT GGTATCTGCG CTCTGCTGAA GCCAGTTACC TTCGGAAAAA GAGTTGGTAG
     2461 CTCTTGATCC GGCAAACAAA CCACCGCTGG TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA
     2521 GATTACGCGC AGAAAAAAAG GATCTCAAGA AGGCCTACTA TTAGCAACAA CGATCCTTTG
     2581 ATCTTTTCTA CGGGGTCTGA CGCTCAGTGG AACGAAAACT CACGTTAAGG GATTTTGGTC
     2641 ATGAGATTAT CAAAAAGGAT CTTCACCTAG ATCCTTTTAA ATTAAAAATG AAGTTTTAAA
     2701 TCAATCTAAA GTATATATGA GTAAACTTGG TCTGACAGTT ACCAATGCTT AATCAGTGAG
     2761 GCACCTATCT CAGCGATCTG TCTATTTCGT TCATCCATAG TTGCCTGACT CCCCGTCGTG
     2821 TAGATAACTA CGATACGGGA GGGCTTACCA TCTGGCCCCA GTGCTGCAAT GATACCGCGA
     2881 GAACCACGCT CACCGGCTCC AGATTTATCA GCAATAAACC AGCCAGCCGG AAGGGCCGAG
     2941 CGCAGAAGTG GTCCTGCAAC TTTATCCGCC TCCATCCAGT CTATTAATTG TTGCCGGGAA
     3001 GCTAGAGTAA GTAGTTCGCC AGTTAATAGT TTGCGCAACG TTGTTGCCAT TGCTACAGGC
     3061 ATCGTGGTGT CACGCTCGTC GTTTGGTATG GCTTCATTCA GCTCCGGTTC CCAACGATCA
     3121 AGGCGAGTTA CATGATCCCC CATGTTGTGC AAAAAAGCGG TTAGCTCCTT CGGTCCTCCG
     3181 ATCGTTGTCA GAAGTAAGTT GGCCGCAGTG TTATCACTCA TGGTTATGGC AGCACTGCAT
     3241 AATTCTCTTA CTGTCATGCC ATCCGTAAGA TGCTTTTCTG TGACTGGTGA GTACTCAACC
     3301 AAGTCATTCT GAGAATAGTG TATGCGGCGA CCGAGTTGCT CTTGCCCGGC GTCAATACGG
     3361 GATAATACCG CGCCACATAG CAGAACTTTA AAAGTGCTCA TCATTGGAAA ACGTTCTTCG
     3421 GGGCGAAAAC TCTCAAGGAT CTTACCGCTG TTGAGATCCA GTTCGATGTA ACCCACTCGT
     3481 GCACCCAACT GATCTTCAGC ATCTTTTACT TTCACCAGCG TTTCTGGGTG AGCAAAAACA
     3541 GGAAGGCAAA ATGCCGCAAA AAAGGGAATA AGGGCGACAC GGAAATGTTG AATACTCATA
     3601 CTCTTCCTTT TTCAATATTA TTGAAGCATT TATCAGGGTT ATTGTCTCAT GAGCGGATAC
     3661 ATATTTGAAT GTATTTAGAA AAATAAACAA ATAGGGGTTC CGCGCACCTG CACCAGTCAG
     3721 TAAAACGACG GCCAGTAGTC AAAAGCCTCC GACCGGAGGC TTTTGACTTG GTTCAGGTGG
     3781 AGTGGGAG
//