LOCUS       Exported     3893 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..3893
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      1930..1977
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     3825..3841
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      3842..3873
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
                     /label="tonB terminator"
     primer_bind     1..20
                     /note="Reverse dialout primer. Unique pair for each FreeGenes part."
                     /label="dialout_for"
     primer_bind     1813..1832
                     /label="dialout_rev"
                     /note="Forward dialout primer. Unique pair for each FreeGenes part."
     CDS             29..1798
                     /label="Bst DNA Polymerase, Large Fragment"
                     /note=""
                     /translation="MTDEGEKPLAGMDFAIADSVTDEMLADKAALVVEVVGDNYHHAPIVGIALANERGRFFLRPETALADPKFLAWLGDETKKKTMFDSKRAAVALKWKGIELRGVVFDLLLAAYLLDPAQAAGDVAAVAKMHQYEAVRSDEAVYGKGAKRTVPDEPTLAEHLARKAAAIWALEEPLMDELRRNEQDRLLTELEQPLAGILANMEFTGVKVDTKRLEQMGAELTEQLQAVERRIYELAGQEFNINSPKQLGTVLFDKLQLPVLKKTKTGYSTSADVLEKLAPHHEIVEHILHYRQLGKLQSTYIEGLLKVVHPVTGKVHTMFNQALTQTGRLSSVEPNLQNIPIRLEEGRKIRQAFVPSEPDWLIFAADYSQIELRVLAHIAEDDNLIEAFRRGLDIHTKTAMDIFHVSEEDVTANMRRQAKAVNFGIVYGISDYGLAQNLNITRKEAAEFIERYFASFPGVKQYMDNIVQEAKQKGYVTTLLHRRRYLPDITSRNFNVRSFAERTAMNTPIQGSAADIIKKAMIDLSVRLREERLQARLLLQVHDELILEAPKEEIERLCRLVPEVMEQAVALRVPLKVDYHYGPTWYDAK*"
     primer_bind     complement(1982..1998)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(2065..2653)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(2844..3704)
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
                     /label="bla"
     promoter        complement(3705..3809)
                     /note="ampicillin resistance gene promoter"
                     /label="bla promoter"
     misc_feature    1833..1892
                     /note="FreeGenes Barcode. Defined as I-SceI site flanked by 21 base pair random sequences."
                     /label="FreeGenes Barcode"
ORIGIN
        1 AAAGGGGCCG TCAATATCAG GGTCTCTAAT GACCGACGAA GGCGAAAAAC CGCTGGCGGG
       61 TATGGACTTT GCGATCGCTG ATAGCGTTAC CGACGAGATG CTGGCAGATA AAGCAGCGCT
      121 AGTAGTTGAA GTTGTGGGCG ATAACTACCA TCATGCGCCG ATCGTGGGTA TCGCTTTGGC
      181 GAACGAGCGC GGCCGTTTTT TCCTGCGCCC GGAAACCGCA CTGGCGGACC CGAAGTTTCT
      241 GGCTTGGCTG GGCGACGAAA CGAAAAAAAA GACTATGTTC GATTCTAAAC GTGCAGCGGT
      301 GGCTCTCAAA TGGAAAGGTA TCGAATTGCG CGGTGTTGTG TTCGACCTGC TGCTGGCTGC
      361 GTACCTGCTC GATCCTGCTC AGGCTGCGGG TGACGTGGCA GCTGTAGCGA AAATGCACCA
      421 GTACGAAGCC GTACGCTCTG ATGAAGCGGT TTATGGTAAA GGTGCGAAAC GTACCGTGCC
      481 AGACGAACCA ACCCTGGCGG AACACCTGGC TCGCAAAGCA GCCGCTATCT GGGCCCTGGA
      541 GGAGCCTCTG ATGGATGAAC TTCGCCGCAA CGAACAGGAC CGTCTGCTCA CAGAACTGGA
      601 ACAGCCGTTG GCGGGTATTC TGGCAAACAT GGAGTTCACT GGTGTAAAAG TGGACACTAA
      661 GCGCCTGGAA CAAATGGGTG CCGAACTGAC TGAGCAGCTT CAGGCAGTTG AACGCCGCAT
      721 CTACGAACTG GCGGGTCAGG AGTTTAACAT CAACTCTCCT AAACAGCTGG GCACCGTGTT
      781 GTTCGACAAA CTGCAACTGC CGGTGTTGAA GAAAACGAAA ACCGGCTACA GCACCTCCGC
      841 CGATGTTCTG GAAAAACTGG CACCGCATCA CGAAATCGTT GAACACATCC TGCACTACCG
      901 TCAGCTGGGT AAACTGCAAT CTACCTACAT CGAGGGCTTA CTGAAAGTAG TGCACCCGGT
      961 GACCGGTAAA GTGCACACTA TGTTCAACCA GGCTCTGACG CAGACCGGCC GCCTGTCCTC
     1021 CGTTGAACCG AACCTGCAAA ACATCCCCAT CCGTCTGGAA GAGGGCCGTA AGATCCGTCA
     1081 GGCATTCGTG CCGTCTGAAC CGGACTGGCT GATTTTCGCT GCTGATTACT CTCAAATCGA
     1141 ACTGCGTGTT CTTGCTCACA TTGCGGAGGA CGATAACCTG ATTGAAGCAT TCCGTCGTGG
     1201 TCTGGACATT CACACTAAAA CGGCTATGGA CATTTTCCAT GTTTCCGAAG AAGATGTGAC
     1261 CGCTAATATG CGTCGTCAGG CTAAAGCGGT AAACTTTGGT ATCGTATACG GTATCTCCGA
     1321 TTACGGCCTG GCACAGAACC TGAACATCAC CCGCAAAGAA GCGGCTGAGT TTATCGAACG
     1381 TTATTTCGCC TCGTTCCCCG GTGTTAAACA GTACATGGAT AACATCGTTC AAGAAGCTAA
     1441 ACAGAAGGGT TATGTGACCA CCCTGCTGCA CCGTCGTCGT TACCTGCCGG ACATCACCTC
     1501 CCGTAACTTC AACGTGCGTT CTTTCGCCGA ACGTACGGCT ATGAATACCC CTATCCAGGG
     1561 CAGCGCGGCG GACATCATCA AAAAAGCCAT GATCGACCTG AGCGTGCGCC TGCGCGAAGA
     1621 ACGTTTGCAG GCTCGCCTGC TTCTGCAAGT GCACGATGAG CTGATCCTAG AAGCTCCAAA
     1681 AGAGGAAATT GAACGTTTGT GCCGTCTGGT TCCGGAAGTT ATGGAACAGG CTGTCGCACT
     1741 GCGTGTTCCG CTTAAAGTTG ATTACCATTA CGGTCCCACT TGGTACGACG CCAAGTGAAG
     1801 AGCTTTGAGA CCACTTCGAT TGGCAAGGAC TGGCCCGTTG CCTATCAGGG TAATAGGGAT
     1861 AACAGGGTAA TAGCAAGAAA TGCAGATACT CTCGCTGAGG TGTCAATCGT CGGAGCCGCT
     1921 GAGCAATAAC TAGCATAACC CCTTGGGGCC TCTAAACGGG TCTTGAGGGG TTTTTTGCAT
     1981 GGTCATAGCT GTTTCCTGAG AGCTTGGCAG GTGATGACAC ACATTAACAA ATTTCGTGAG
     2041 GAGTCTCCAG AAGAATGCCA TTAATTTCCA TAGGCTCCGC CCCCCTGACG AGCATCACAA
     2101 AAATCGACGC TCAAGTCAGA GGTGGCGAAA CCCGACAGGA CTATAAAGAT ACCAGGCGTT
     2161 TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC TGTTCCGACC CTGCCGCTTA CCGGATACCT
     2221 GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC GCTTTCTCAT AGCTCACGCT GTAGGTATCT
     2281 CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT GGGCTGTGTG CACGAACCCC CCGTTCAGCC
     2341 CGACCGCTGC GCCTTATCCG GTAACTATCG TCTTGAGTCC AACCCGGTAA GACACGACTT
     2401 ATCGCCACTG GCAGCAGCCA CTGGTAACAG GATTAGCAGA GCGAGGTATG TAGGCGGTGC
     2461 TACAGAGTTC TTGAAGTGGT GGCCTAACTA CGGCTACACT AGAAGAACAG TATTTGGTAT
     2521 CTGCGCTCTG CTGAAGCCAG TTACCTTCGG AAAAAGAGTT GGTAGCTCTT GATCCGGCAA
     2581 ACAAACCACC GCTGGTAGCG GTGGTTTTTT TGTTTGCAAG CAGCAGATTA CGCGCAGAAA
     2641 AAAAGGATCT CAAGAAGGCC TACTATTAGC AACAACGATC CTTTGATCTT TTCTACGGGG
     2701 TCTGACGCTC AGTGGAACGA AAACTCACGT TAAGGGATTT TGGTCATGAG ATTATCAAAA
     2761 AGGATCTTCA CCTAGATCCT TTTAAATTAA AAATGAAGTT TTAAATCAAT CTAAAGTATA
     2821 TATGAGTAAA CTTGGTCTGA CAGTTACCAA TGCTTAATCA GTGAGGCACC TATCTCAGCG
     2881 ATCTGTCTAT TTCGTTCATC CATAGTTGCC TGACTCCCCG TCGTGTAGAT AACTACGATA
     2941 CGGGAGGGCT TACCATCTGG CCCCAGTGCT GCAATGATAC CGCGAGAACC ACGCTCACCG
     3001 GCTCCAGATT TATCAGCAAT AAACCAGCCA GCCGGAAGGG CCGAGCGCAG AAGTGGTCCT
     3061 GCAACTTTAT CCGCCTCCAT CCAGTCTATT AATTGTTGCC GGGAAGCTAG AGTAAGTAGT
     3121 TCGCCAGTTA ATAGTTTGCG CAACGTTGTT GCCATTGCTA CAGGCATCGT GGTGTCACGC
     3181 TCGTCGTTTG GTATGGCTTC ATTCAGCTCC GGTTCCCAAC GATCAAGGCG AGTTACATGA
     3241 TCCCCCATGT TGTGCAAAAA AGCGGTTAGC TCCTTCGGTC CTCCGATCGT TGTCAGAAGT
     3301 AAGTTGGCCG CAGTGTTATC ACTCATGGTT ATGGCAGCAC TGCATAATTC TCTTACTGTC
     3361 ATGCCATCCG TAAGATGCTT TTCTGTGACT GGTGAGTACT CAACCAAGTC ATTCTGAGAA
     3421 TAGTGTATGC GGCGACCGAG TTGCTCTTGC CCGGCGTCAA TACGGGATAA TACCGCGCCA
     3481 CATAGCAGAA CTTTAAAAGT GCTCATCATT GGAAAACGTT CTTCGGGGCG AAAACTCTCA
     3541 AGGATCTTAC CGCTGTTGAG ATCCAGTTCG ATGTAACCCA CTCGTGCACC CAACTGATCT
     3601 TCAGCATCTT TTACTTTCAC CAGCGTTTCT GGGTGAGCAA AAACAGGAAG GCAAAATGCC
     3661 GCAAAAAAGG GAATAAGGGC GACACGGAAA TGTTGAATAC TCATACTCTT CCTTTTTCAA
     3721 TATTATTGAA GCATTTATCA GGGTTATTGT CTCATGAGCG GATACATATT TGAATGTATT
     3781 TAGAAAAATA AACAAATAGG GGTTCCGCGC ACCTGCACCA GTCAGTAAAA CGACGGCCAG
     3841 TAGTCAAAAG CCTCCGACCG GAGGCTTTTG ACTTGGTTCA GGTGGAGTGG GAG
//