LOCUS       Exported     3851 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..3851
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      1888..1935
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     3783..3799
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      3800..3831
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     primer_bind     1..20
                     /label="dialout_for"
                     /note="Reverse dialout primer. Unique pair for each FreeGenes part."
     primer_bind     1771..1790
                     /label="dialout_rev"
                     /note="Forward dialout primer. Unique pair for each FreeGenes part."
     CDS             29..1756
                     /label="phi29 DNA Polymerase"
                     /note=""
                     /translation="MKHMPRKMYSCDFETTTKVEDCRVWAYGYMNIEDHSEYKIGNSLDEFMAWVLKVQADLYFHNLKFDGAFIINWLERNGFKWSADGLPNTYNTIISRMGQWYMIDICLGYKGKRKIHTVIYDSLKKLPFPVKKIAKDFKLTVLKGDIDYHKERPVGYKITPEEYAYIKNDIQIIAERLLIQFKQGLDRMTAGSDSLKGFKDIITTKKFKKVFPTLSLGLDKEVRYAYRGGFTWLNDRFKEKEIGEGMVFDVNSLYPAQMYSRLLPYGEPIVFEGKYVWDEDYPLHIQHIRCEFELKEGYIPTIQIKRSRFYKGNEYLKSSGGEIADLWLSNVDLELMKEHYDLYNVEYISGLKFKATTGLFKDFIDKWTYIKTTSEGAIKQLAKLMLNSLYGKFASNPDVTGKVPYLKENGALGFRLGEEETKDPVYTPMGVFITAWARYTTITAAQACYDRIIYCDTDSIHLTGTEIPDVIKDIVDPKKLGYWAHESTFKRVKYLRQKTYIQDIYMKEVDGKLVEGSPDDYTDIKFSVKCAGMTDKIKKEVTFENFKVGFSRKMKPKPVQVPGGVVLVDDTFTIK*"
     primer_bind     complement(1940..1956)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(2023..2611)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(2802..3662)
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
     promoter        complement(3663..3767)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
     misc_feature    1791..1850
                     /note="FreeGenes Barcode. Defined as I-SceI site flanked by 21 base pair random sequences."
                     /label="FreeGenes Barcode"
ORIGIN
        1 AAAGGGGCCG TCAATATCAG GGTCTCTAAT GAAACACATG CCACGCAAGA TGTATTCTTG
       61 CGACTTCGAA ACGACCACCA AAGTGGAAGA TTGCCGCGTA TGGGCTTACG GTTACATGAA
      121 CATTGAGGAC CACTCCGAAT ACAAAATCGG CAACAGTCTG GACGAGTTCA TGGCGTGGGT
      181 GCTTAAAGTG CAGGCTGACC TGTACTTCCA CAATCTGAAG TTTGATGGTG CGTTCATCAT
      241 CAACTGGCTG GAACGCAACG GGTTCAAATG GAGCGCTGAT GGCCTGCCGA ACACCTATAA
      301 CACTATTATC AGCCGTATGG GTCAGTGGTA CATGATCGAC ATCTGCCTGG GCTACAAAGG
      361 CAAACGTAAA ATCCACACTG TTATCTACGA TTCTCTGAAA AAGCTGCCGT TCCCGGTTAA
      421 AAAAATTGCG AAAGACTTCA AGCTGACCGT TCTCAAAGGC GATATCGACT ATCACAAAGA
      481 GCGTCCGGTA GGCTATAAAA TCACGCCTGA GGAATATGCG TATATTAAAA ACGATATCCA
      541 GATCATTGCA GAACGTCTGC TGATCCAGTT TAAACAGGGT CTGGATCGTA TGACGGCCGG
      601 TAGTGATTCG CTGAAAGGCT TCAAAGACAT TATTACCACC AAGAAGTTCA AAAAAGTTTT
      661 CCCAACACTG TCCTTAGGCT TGGACAAAGA AGTGCGTTAT GCGTACCGTG GTGGTTTTAC
      721 CTGGCTGAAC GACCGTTTTA AAGAAAAAGA GATCGGCGAG GGCATGGTGT TTGATGTTAA
      781 CAGCCTGTAC CCAGCGCAGA TGTATAGCCG CCTGCTCCCG TACGGCGAAC CGATCGTTTT
      841 TGAAGGCAAG TATGTTTGGG ACGAAGATTA TCCGCTTCAC ATCCAGCATA TTCGCTGCGA
      901 GTTCGAACTG AAGGAAGGTT ACATCCCGAC GATCCAAATC AAACGTTCTC GTTTTTACAA
      961 GGGTAACGAG TACCTAAAAA GCTCCGGCGG CGAAATCGCG GACCTGTGGC TTAGCAATGT
     1021 GGACCTGGAG CTGATGAAGG AACACTATGA CCTGTACAAC GTTGAATACA TCTCTGGTCT
     1081 GAAGTTCAAA GCAACCACCG GTCTGTTCAA AGACTTCATC GATAAATGGA CTTATATCAA
     1141 AACTACAAGT GAAGGTGCGA TTAAACAACT CGCCAAACTG ATGCTGAACT CTCTGTATGG
     1201 CAAGTTCGCC AGCAACCCGG ACGTTACTGG TAAAGTTCCA TATCTGAAAG AAAACGGCGC
     1261 TCTGGGCTTC CGCCTCGGTG AAGAAGAAAC CAAAGACCCG GTCTACACAC CGATGGGAGT
     1321 ATTCATCACC GCGTGGGCAC GTTACACTAC TATCACTGCG GCCCAGGCGT GCTATGATCG
     1381 CATCATCTAC TGCGACACTG ATTCCATCCA CCTGACTGGC ACCGAGATTC CAGATGTAAT
     1441 CAAAGATATC GTGGACCCGA AAAAACTGGG TTACTGGGCA CACGAATCCA CCTTCAAACG
     1501 CGTGAAATAT CTGCGTCAAA AGACCTATAT CCAGGATATC TACATGAAAG AAGTGGATGG
     1561 TAAGCTGGTC GAAGGCTCCC CGGACGACTA CACCGACATT AAGTTCTCCG TGAAATGCGC
     1621 TGGCATGACC GATAAAATCA AAAAAGAAGT TACCTTCGAA AACTTCAAGG TGGGCTTCAG
     1681 CCGTAAAATG AAACCGAAGC CTGTTCAGGT GCCGGGCGGC GTGGTTCTGG TCGATGATAC
     1741 TTTTACGATC AAGTGAAGAG CTTTGAGACC AGACAACAAT CTGAGGCTGG GGTATTGGAT
     1801 GATTCTAGGG GTAGGGATAA CAGGGTAATC CAGTATACTA TATGATTCGG CGCTGAGGTG
     1861 TCAATCGTCG GAGCCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC TAAACGGGTC
     1921 TTGAGGGGTT TTTTGCATGG TCATAGCTGT TTCCTGAGAG CTTGGCAGGT GATGACACAC
     1981 ATTAACAAAT TTCGTGAGGA GTCTCCAGAA GAATGCCATT AATTTCCATA GGCTCCGCCC
     2041 CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC CGACAGGACT
     2101 ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG TTCCGACCCT
     2161 GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC TTTCTCATAG
     2221 CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA
     2281 CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC TTGAGTCCAA
     2341 CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA TTAGCAGAGC
     2401 GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG GCTACACTAG
     2461 AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG
     2521 TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA
     2581 GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGGCCTA CTATTAGCAA CAACGATCCT
     2641 TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA AGGGATTTTG
     2701 GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA ATGAAGTTTT
     2761 AAATCAATCT AAAGTATATA TGAGTAAACT TGGTCTGACA GTTACCAATG CTTAATCAGT
     2821 GAGGCACCTA TCTCAGCGAT CTGTCTATTT CGTTCATCCA TAGTTGCCTG ACTCCCCGTC
     2881 GTGTAGATAA CTACGATACG GGAGGGCTTA CCATCTGGCC CCAGTGCTGC AATGATACCG
     2941 CGAGAACCAC GCTCACCGGC TCCAGATTTA TCAGCAATAA ACCAGCCAGC CGGAAGGGCC
     3001 GAGCGCAGAA GTGGTCCTGC AACTTTATCC GCCTCCATCC AGTCTATTAA TTGTTGCCGG
     3061 GAAGCTAGAG TAAGTAGTTC GCCAGTTAAT AGTTTGCGCA ACGTTGTTGC CATTGCTACA
     3121 GGCATCGTGG TGTCACGCTC GTCGTTTGGT ATGGCTTCAT TCAGCTCCGG TTCCCAACGA
     3181 TCAAGGCGAG TTACATGATC CCCCATGTTG TGCAAAAAAG CGGTTAGCTC CTTCGGTCCT
     3241 CCGATCGTTG TCAGAAGTAA GTTGGCCGCA GTGTTATCAC TCATGGTTAT GGCAGCACTG
     3301 CATAATTCTC TTACTGTCAT GCCATCCGTA AGATGCTTTT CTGTGACTGG TGAGTACTCA
     3361 ACCAAGTCAT TCTGAGAATA GTGTATGCGG CGACCGAGTT GCTCTTGCCC GGCGTCAATA
     3421 CGGGATAATA CCGCGCCACA TAGCAGAACT TTAAAAGTGC TCATCATTGG AAAACGTTCT
     3481 TCGGGGCGAA AACTCTCAAG GATCTTACCG CTGTTGAGAT CCAGTTCGAT GTAACCCACT
     3541 CGTGCACCCA ACTGATCTTC AGCATCTTTT ACTTTCACCA GCGTTTCTGG GTGAGCAAAA
     3601 ACAGGAAGGC AAAATGCCGC AAAAAAGGGA ATAAGGGCGA CACGGAAATG TTGAATACTC
     3661 ATACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT CATGAGCGGA
     3721 TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC CTGCACCAGT
     3781 CAGTAAAACG ACGGCCAGTA GTCAAAAGCC TCCGACCGGA GGCTTTTGAC TTGGTTCAGG
     3841 TGGAGTGGGA G
//