LOCUS       Exported     3182 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..3182
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      1219..1266
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     3114..3130
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      3131..3162
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     primer_bind     1..20
                     /label="dialout_for"
                     /note="Reverse dialout primer. Unique pair for each FreeGenes part."
     primer_bind     1102..1121
                     /note="Forward dialout primer. Unique pair for each FreeGenes part."
                     /label="dialout_rev"
     CDS             29..1087
                     /translation="MIVLFVDFDYFYAQVEEVLNPSLKGKPVVVCVFSGRFEDSGAVATANYEARKFGVKAGIPIVEAKKILPNAVYLPMRKEVYQQVSSRIMNLLREYSEKIEIASIDEAYLDISDKVRDYREAYNLGLEIKNKILEKEKITVTVGISKNKVFAKIAADMAKPNGIKVIDDEEVKRLIRELDIADVPGIGNITAEKLKKLGINKLVDTLSIEFDKLKGMIGEAKAKYLISLARDEYNEPIRTRVRKSIGRIVTMKRNSRNLEEIKPYLFRAIEESYYKLDKRIPKAIHVVAVTEDLDIVSRGRTFPHGISKETAYSESVKLLQKILEEDERKIRRIGVRFSKFIEAIGLDKFFDT*"
                     /label="Sulfolobus DNA Polymerase IV"
                     /note=""
     primer_bind     complement(1271..1287)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(1354..1942)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(2133..2993)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(2994..3098)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
     misc_feature    1122..1181
                     /label="FreeGenes Barcode"
                     /note="FreeGenes Barcode. Defined as I-SceI site flanked by 21 base pair random sequences."
ORIGIN
        1 AAAGGGGCCG TCAATATCAG GGTCTCTAAT GATCGTTCTG TTCGTCGACT TCGACTACTT
       61 TTATGCCCAG GTGGAAGAAG TACTGAACCC GTCGCTGAAA GGTAAACCGG TAGTTGTTTG
      121 CGTTTTTAGC GGTCGTTTTG AGGACAGCGG TGCTGTTGCA ACCGCTAATT ATGAAGCCCG
      181 TAAATTTGGT GTTAAAGCAG GTATCCCGAT TGTTGAAGCC AAAAAAATCC TGCCGAACGC
      241 TGTGTATCTG CCGATGCGTA AAGAAGTTTA CCAACAGGTG TCCTCCCGTA TTATGAACTT
      301 GCTGCGTGAA TATTCTGAAA AAATCGAGAT TGCGTCAATC GATGAGGCGT ACCTGGACAT
      361 CAGCGACAAA GTGCGTGACT ACCGTGAAGC ATATAACCTG GGTCTGGAAA TCAAAAACAA
      421 AATTCTGGAG AAAGAAAAGA TCACGGTTAC TGTGGGTATC AGCAAAAACA AAGTATTCGC
      481 TAAAATTGCG GCGGATATGG CAAAGCCGAA CGGTATCAAA GTTATCGACG ACGAAGAGGT
      541 GAAACGCCTG ATCCGTGAAC TGGATATTGC TGACGTCCCT GGTATTGGTA ACATCACTGC
      601 TGAAAAACTT AAAAAACTGG GTATTAACAA ACTGGTGGAC ACTCTGTCTA TCGAGTTCGA
      661 TAAACTTAAA GGCATGATCG GTGAGGCAAA AGCTAAATAC CTGATTAGCC TGGCCCGTGA
      721 CGAATACAAC GAACCGATCC GTACCCGTGT GCGCAAGTCT ATCGGCCGTA TCGTAACTAT
      781 GAAGCGTAAT TCCCGTAACC TGGAAGAAAT CAAGCCTTAT CTGTTTCGCG CGATCGAAGA
      841 ATCCTATTAT AAACTGGACA AGCGTATCCC AAAAGCCATC CACGTGGTGG CGGTTACCGA
      901 GGACCTGGAC ATTGTCAGTC GCGGTCGTAC TTTCCCGCAC GGCATCTCTA AAGAAACCGC
      961 GTACTCTGAA AGCGTAAAGC TGCTGCAAAA AATTCTGGAA GAGGACGAAC GTAAAATTCG
     1021 CCGCATCGGC GTTCGTTTTT CCAAATTCAT CGAAGCGATT GGTCTGGACA AATTCTTTGA
     1081 CACCTGAAGA GCTTTGAGAC CAGACACAAG GCTGATTCCA GAATAGCGCG GCTAGATAGG
     1141 ATTAGGGATA ACAGGGTAAT CAAATCCGAT ATATTCCCTC GCGCTGAGGT GTCAATCGTC
     1201 GGAGCCGCTG AGCAATAACT AGCATAACCC CTTGGGGCCT CTAAACGGGT CTTGAGGGGT
     1261 TTTTTGCATG GTCATAGCTG TTTCCTGAGA GCTTGGCAGG TGATGACACA CATTAACAAA
     1321 TTTCGTGAGG AGTCTCCAGA AGAATGCCAT TAATTTCCAT AGGCTCCGCC CCCCTGACGA
     1381 GCATCACAAA AATCGACGCT CAAGTCAGAG GTGGCGAAAC CCGACAGGAC TATAAAGATA
     1441 CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT GCGCTCTCCT GTTCCGACCC TGCCGCTTAC
     1501 CGGATACCTG TCCGCCTTTC TCCCTTCGGG AAGCGTGGCG CTTTCTCATA GCTCACGCTG
     1561 TAGGTATCTC AGTTCGGTGT AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC ACGAACCCCC
     1621 CGTTCAGCCC GACCGCTGCG CCTTATCCGG TAACTATCGT CTTGAGTCCA ACCCGGTAAG
     1681 ACACGACTTA TCGCCACTGG CAGCAGCCAC TGGTAACAGG ATTAGCAGAG CGAGGTATGT
     1741 AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG GCCTAACTAC GGCTACACTA GAAGAACAGT
     1801 ATTTGGTATC TGCGCTCTGC TGAAGCCAGT TACCTTCGGA AAAAGAGTTG GTAGCTCTTG
     1861 ATCCGGCAAA CAAACCACCG CTGGTAGCGG TGGTTTTTTT GTTTGCAAGC AGCAGATTAC
     1921 GCGCAGAAAA AAAGGATCTC AAGAAGGCCT ACTATTAGCA ACAACGATCC TTTGATCTTT
     1981 TCTACGGGGT CTGACGCTCA GTGGAACGAA AACTCACGTT AAGGGATTTT GGTCATGAGA
     2041 TTATCAAAAA GGATCTTCAC CTAGATCCTT TTAAATTAAA AATGAAGTTT TAAATCAATC
     2101 TAAAGTATAT ATGAGTAAAC TTGGTCTGAC AGTTACCAAT GCTTAATCAG TGAGGCACCT
     2161 ATCTCAGCGA TCTGTCTATT TCGTTCATCC ATAGTTGCCT GACTCCCCGT CGTGTAGATA
     2221 ACTACGATAC GGGAGGGCTT ACCATCTGGC CCCAGTGCTG CAATGATACC GCGAGAACCA
     2281 CGCTCACCGG CTCCAGATTT ATCAGCAATA AACCAGCCAG CCGGAAGGGC CGAGCGCAGA
     2341 AGTGGTCCTG CAACTTTATC CGCCTCCATC CAGTCTATTA ATTGTTGCCG GGAAGCTAGA
     2401 GTAAGTAGTT CGCCAGTTAA TAGTTTGCGC AACGTTGTTG CCATTGCTAC AGGCATCGTG
     2461 GTGTCACGCT CGTCGTTTGG TATGGCTTCA TTCAGCTCCG GTTCCCAACG ATCAAGGCGA
     2521 GTTACATGAT CCCCCATGTT GTGCAAAAAA GCGGTTAGCT CCTTCGGTCC TCCGATCGTT
     2581 GTCAGAAGTA AGTTGGCCGC AGTGTTATCA CTCATGGTTA TGGCAGCACT GCATAATTCT
     2641 CTTACTGTCA TGCCATCCGT AAGATGCTTT TCTGTGACTG GTGAGTACTC AACCAAGTCA
     2701 TTCTGAGAAT AGTGTATGCG GCGACCGAGT TGCTCTTGCC CGGCGTCAAT ACGGGATAAT
     2761 ACCGCGCCAC ATAGCAGAAC TTTAAAAGTG CTCATCATTG GAAAACGTTC TTCGGGGCGA
     2821 AAACTCTCAA GGATCTTACC GCTGTTGAGA TCCAGTTCGA TGTAACCCAC TCGTGCACCC
     2881 AACTGATCTT CAGCATCTTT TACTTTCACC AGCGTTTCTG GGTGAGCAAA AACAGGAAGG
     2941 CAAAATGCCG CAAAAAAGGG AATAAGGGCG ACACGGAAAT GTTGAATACT CATACTCTTC
     3001 CTTTTTCAAT ATTATTGAAG CATTTATCAG GGTTATTGTC TCATGAGCGG ATACATATTT
     3061 GAATGTATTT AGAAAAATAA ACAAATAGGG GTTCCGCGCA CCTGCACCAG TCAGTAAAAC
     3121 GACGGCCAGT AGTCAAAAGC CTCCGACCGG AGGCTTTTGA CTTGGTTCAG GTGGAGTGGG
     3181 AG
//