LOCUS Exported 4820 bp DNA circular SYN 24-JUL-2020 DEFINITION synthetic linear DNA ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2001) AUTHORS Keoni Gandall TITLE Direct Submission JOURNAL Exported from Poly. FEATURES Location/Qualifiers terminator 1..4820 /label="T7 terminator" /note="transcription terminator for bacteriophage T7 RNA polymerase" terminator 2857..2904 /label="T7 terminator" /note="transcription terminator for bacteriophage T7 RNA polymerase" primer_bind 4752..4768 /label="M13 for" /note="common sequencing primer, one of multiple similar variants" terminator 4769..4800 /label="tonB terminator" /note="bidirectional E. coli tonB-P14 transcription terminator" primer_bind 1..20 /label="dialout_for" /note="Reverse dialout primer. Unique pair for each FreeGenes part." primer_bind 2740..2759 /label="dialout_rev" /note="Forward dialout primer. Unique pair for each FreeGenes part." CDS 29..2725 /label="T4 DNA Polymerase" /note="" /translation="MKEFYISIETVGNNIVERYIDENGKERTREVEYLPTMFRHCKEESKYKDIYGKNCAPQKFPSMKDARDWMKRMEDIGLEALGMNDFKLAYISDTYGSEIVYDRKFVRVANCDIEVTGDKFPDPMKAEYEIDAITHYDSIDDRFYVFDLLNSMYGSVSKWDAKLAAKLDCEGGDEVPQEILDRVIYMPFDNERDMLMEYINLWEQKRPAIFTGWNIEGFDVPYIMNRVKMILGERSMKRFSPIGRVKSKLIQNMYGSKEIYSIDGVSILDYLDLYKKFAFTNLPSFSLESVAQHETKKGKLPYDGPINKLRETNHQRYISYNIIDVESVQAIDKIRGFIDLVLSMSYYAKMPFSGVMSPIKTWDAIIFNSLKGEHKVIPQQGSHVKQSFPGAFVFEPKPIARRYIMSFDLTSLYPSIIRQVNISPETIRGQFKVHPIHEYIAGTAPKPSDEYSCSPNGWMYDKHQEGIIPKEIAKVFFQRKDWKKKMFAEEMNAEAIKKIIMKGAGSCSTKPEVERYVKFSDDFLNELSNYTESVLNSLIEECEKAATLANTNQLNRKILINSLYGALGNIHFRYYDLRNATAITIFGQVGIQWIARKINEYLNKVCGTNDEDFIAAGDTDSVYVCVDKVIEKVGLDRFKEQNDLVEFMNQFGKKKMEPMIDVAYRELCDYMNNREHLMHMDREAISCPPLGSKGVGGFWKAKKRYALNVYDMEDKRFAEPHLKIMGMETQQSSTPKAVQEALEESIRRILQEGEESVQEYYKNFEKEYRQLDYKVIAEVKTANDIAKYDDKGWPGFKCPFHIRGVLTYRRAVSGLGVAPILDGNKVMVLPLREGNPFGDKCIAWPSGTELPKEIRSDVLSWIDHSTLFQKSFVKPLAGMCESAGMDYEEKASLDFLFG*" primer_bind complement(2909..2925) /label="M13 rev" /note="common sequencing primer, one of multiple similar variants" rep_origin complement(2992..3580) /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(3771..4631) /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*" /label="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" promoter complement(4632..4736) /label="bla promoter" /note="ampicillin resistance gene promoter" misc_feature 2760..2819 /label="FreeGenes Barcode" /note="FreeGenes Barcode. Defined as I-SceI site flanked by 21 base pair random sequences." ORIGIN 1 AAAGGGGCCG TCAATATCAG GGTCTCTAAT GAAAGAGTTC TACATCTCCA TCGAAACCGT 61 TGGTAACAAT ATCGTTGAAC GCTACATTGA TGAAAACGGT AAAGAACGTA CCCGCGAGGT 121 AGAATACCTC CCGACCATGT TCCGTCACTG CAAAGAGGAA TCTAAATACA AAGATATTTA 181 CGGTAAGAAC TGCGCTCCGC AGAAGTTCCC GTCCATGAAA GACGCACGTG ACTGGATGAA 241 ACGTATGGAG GACATCGGCC TCGAAGCTCT GGGCATGAAC GACTTCAAAC TCGCGTACAT 301 CTCGGACACC TACGGCTCCG AAATCGTGTA CGATCGTAAG TTCGTGCGCG TAGCGAATTG 361 CGATATCGAA GTCACCGGTG ACAAGTTCCC AGATCCGATG AAAGCAGAAT ACGAAATTGA 421 TGCAATCACC CATTATGATA GCATCGATGA CCGTTTCTAC GTATTCGATC TGCTGAACTC 481 CATGTATGGT TCTGTTTCCA AGTGGGACGC TAAGCTTGCC GCCAAACTGG ATTGTGAAGG 541 TGGTGACGAG GTGCCACAGG AAATCCTGGA TCGTGTTATC TATATGCCGT TCGATAACGA 601 ACGTGACATG CTGATGGAAT ACATTAACCT CTGGGAACAG AAACGCCCGG CTATTTTCAC 661 CGGTTGGAAC ATCGAAGGTT TCGATGTTCC GTACATCATG AATCGCGTGA AAATGATTCT 721 GGGTGAGCGT AGCATGAAGC GCTTCTCTCC GATTGGTCGT GTTAAATCGA AACTGATCCA 781 GAACATGTAC GGCTCTAAAG AAATCTACTC GATCGATGGC GTGTCTATTC TGGACTACCT 841 GGATCTTTAC AAGAAGTTCG CATTCACCAA CCTGCCGTCC TTCTCTCTGG AATCCGTAGC 901 ACAGCACGAA ACCAAAAAAG GCAAGCTGCC GTATGACGGC CCAATCAACA AACTGCGTGA 961 AACTAATCAC CAGCGTTACA TTTCGTACAA CATTATTGAC GTTGAATCTG TACAGGCTAT 1021 CGATAAAATC CGTGGCTTCA TCGATCTGGT TCTGAGCATG TCGTACTACG CTAAAATGCC 1081 GTTCAGCGGT GTTATGTCTC CGATCAAGAC CTGGGATGCC ATCATCTTTA ACTCCCTGAA 1141 AGGCGAACAC AAAGTTATTC CGCAGCAGGG TTCCCACGTT AAACAGTCTT TCCCGGGCGC 1201 ATTTGTTTTC GAACCTAAAC CGATCGCGCG CCGTTACATC ATGTCCTTTG ACCTGACCAG 1261 CCTGTACCCT AGCATCATCC GTCAAGTGAA CATCAGCCCA GAAACCATTC GCGGTCAATT 1321 CAAAGTCCAT CCGATCCACG AATATATCGC TGGTACCGCG CCAAAACCGA GCGACGAATA 1381 CTCATGCTCG CCGAACGGTT GGATGTATGA TAAACATCAG GAGGGCATCA TTCCGAAAGA 1441 AATCGCCAAA GTGTTCTTTC AACGTAAAGA CTGGAAAAAG AAAATGTTCG CGGAAGAAAT 1501 GAACGCGGAA GCGATCAAAA AAATCATCAT GAAGGGTGCT GGCTCTTGCT CGACCAAGCC 1561 GGAAGTGGAA CGTTACGTGA AGTTTAGTGA TGATTTTCTG AACGAGCTGT CCAACTACAC 1621 TGAATCCGTC CTGAACTCAC TGATCGAGGA GTGTGAAAAA GCAGCGACGC TGGCGAACAC 1681 CAACCAGCTG AACCGTAAAA TCCTGATCAA CAGCCTATAC GGTGCACTGG GTAACATCCA 1741 CTTCCGTTAC TACGATCTGC GCAACGCCAC TGCGATTACC ATCTTCGGGC AGGTGGGAAT 1801 CCAGTGGATC GCCCGTAAAA TTAACGAATA CCTGAACAAA GTTTGTGGTA CCAACGACGA 1861 GGATTTCATT GCAGCTGGCG ACACGGATTC CGTATATGTC TGCGTAGACA AGGTGATTGA 1921 GAAAGTGGGT CTGGACCGTT TTAAAGAACA GAACGACCTG GTCGAGTTCA TGAATCAGTT 1981 TGGCAAAAAG AAAATGGAAC CGATGATCGA TGTCGCATAC CGCGAACTGT GTGATTACAT 2041 GAACAACCGC GAACATCTGA TGCACATGGA CCGTGAAGCC ATATCTTGTC CTCCACTGGG 2101 CTCCAAAGGT GTCGGTGGCT TCTGGAAGGC GAAAAAACGT TATGCCCTGA ACGTCTATGA 2161 TATGGAGGAC AAACGCTTCG CGGAGCCTCA CCTGAAAATT ATGGGCATGG AAACTCAGCA 2221 AAGCAGCACT CCGAAAGCAG TTCAAGAGGC TCTGGAAGAA AGCATTCGCC GCATTCTGCA 2281 AGAGGGTGAA GAATCGGTTC AGGAATACTA CAAAAACTTC GAAAAAGAGT ACCGCCAACT 2341 GGATTACAAA GTTATCGCCG AGGTTAAAAC CGCTAACGAT ATCGCTAAGT ACGATGATAA 2401 AGGTTGGCCG GGCTTTAAAT GCCCTTTTCA CATCCGTGGT GTTCTGACAT ACCGTCGTGC 2461 TGTTTCTGGT CTGGGCGTAG CTCCGATTCT GGACGGTAAC AAAGTTATGG TACTTCCGCT 2521 GCGTGAAGGT AACCCGTTCG GTGATAAATG TATCGCGTGG CCGTCTGGTA CCGAACTGCC 2581 GAAGGAAATC CGTTCGGATG TACTGTCTTG GATTGATCAC TCTACCCTGT TCCAAAAATC 2641 CTTCGTTAAA CCGCTGGCCG GCATGTGCGA ATCCGCAGGA ATGGACTACG AAGAAAAGGC 2701 CTCCTTAGAC TTTCTCTTCG GCTGAAGAGC TTTGAGACCA GACATGGGAT TGACCACACC 2761 ATCGAGCGTA GGGCCAACCC TAGGGATAAC AGGGTAATCT GAATTACTTC GCGCGTGATC 2821 GCTGAGGTGT CAATCGTCGG AGCCGCTGAG CAATAACTAG CATAACCCCT TGGGGCCTCT 2881 AAACGGGTCT TGAGGGGTTT TTTGCATGGT CATAGCTGTT TCCTGAGAGC TTGGCAGGTG 2941 ATGACACACA TTAACAAATT TCGTGAGGAG TCTCCAGAAG AATGCCATTA ATTTCCATAG 3001 GCTCCGCCCC CCTGACGAGC ATCACAAAAA TCGACGCTCA AGTCAGAGGT GGCGAAACCC 3061 GACAGGACTA TAAAGATACC AGGCGTTTCC CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT 3121 TCCGACCCTG CCGCTTACCG GATACCTGTC CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT 3181 TTCTCATAGC TCACGCTGTA GGTATCTCAG TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG 3241 CTGTGTGCAC GAACCCCCCG TTCAGCCCGA CCGCTGCGCC TTATCCGGTA ACTATCGTCT 3301 TGAGTCCAAC CCGGTAAGAC ACGACTTATC GCCACTGGCA GCAGCCACTG GTAACAGGAT 3361 TAGCAGAGCG AGGTATGTAG GCGGTGCTAC AGAGTTCTTG AAGTGGTGGC CTAACTACGG 3421 CTACACTAGA AGAACAGTAT TTGGTATCTG CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA 3481 AAGAGTTGGT AGCTCTTGAT CCGGCAAACA AACCACCGCT GGTAGCGGTG GTTTTTTTGT 3541 TTGCAAGCAG CAGATTACGC GCAGAAAAAA AGGATCTCAA GAAGGCCTAC TATTAGCAAC 3601 AACGATCCTT TGATCTTTTC TACGGGGTCT GACGCTCAGT GGAACGAAAA CTCACGTTAA 3661 GGGATTTTGG TCATGAGATT ATCAAAAAGG ATCTTCACCT AGATCCTTTT AAATTAAAAA 3721 TGAAGTTTTA AATCAATCTA AAGTATATAT GAGTAAACTT GGTCTGACAG TTACCAATGC 3781 TTAATCAGTG AGGCACCTAT CTCAGCGATC TGTCTATTTC GTTCATCCAT AGTTGCCTGA 3841 CTCCCCGTCG TGTAGATAAC TACGATACGG GAGGGCTTAC CATCTGGCCC CAGTGCTGCA 3901 ATGATACCGC GAGAACCACG CTCACCGGCT CCAGATTTAT CAGCAATAAA CCAGCCAGCC 3961 GGAAGGGCCG AGCGCAGAAG TGGTCCTGCA ACTTTATCCG CCTCCATCCA GTCTATTAAT 4021 TGTTGCCGGG AAGCTAGAGT AAGTAGTTCG CCAGTTAATA GTTTGCGCAA CGTTGTTGCC 4081 ATTGCTACAG GCATCGTGGT GTCACGCTCG TCGTTTGGTA TGGCTTCATT CAGCTCCGGT 4141 TCCCAACGAT CAAGGCGAGT TACATGATCC CCCATGTTGT GCAAAAAAGC GGTTAGCTCC 4201 TTCGGTCCTC CGATCGTTGT CAGAAGTAAG TTGGCCGCAG TGTTATCACT CATGGTTATG 4261 GCAGCACTGC ATAATTCTCT TACTGTCATG CCATCCGTAA GATGCTTTTC TGTGACTGGT 4321 GAGTACTCAA CCAAGTCATT CTGAGAATAG TGTATGCGGC GACCGAGTTG CTCTTGCCCG 4381 GCGTCAATAC GGGATAATAC CGCGCCACAT AGCAGAACTT TAAAAGTGCT CATCATTGGA 4441 AAACGTTCTT CGGGGCGAAA ACTCTCAAGG ATCTTACCGC TGTTGAGATC CAGTTCGATG 4501 TAACCCACTC GTGCACCCAA CTGATCTTCA GCATCTTTTA CTTTCACCAG CGTTTCTGGG 4561 TGAGCAAAAA CAGGAAGGCA AAATGCCGCA AAAAAGGGAA TAAGGGCGAC ACGGAAATGT 4621 TGAATACTCA TACTCTTCCT TTTTCAATAT TATTGAAGCA TTTATCAGGG TTATTGTCTC 4681 ATGAGCGGAT ACATATTTGA ATGTATTTAG AAAAATAAAC AAATAGGGGT TCCGCGCACC 4741 TGCACCAGTC AGTAAAACGA CGGCCAGTAG TCAAAAGCCT CCGACCGGAG GCTTTTGACT 4801 TGGTTCAGGT GGAGTGGGAG //