LOCUS       Exported     4238 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..4238
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      2275..2322
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     4170..4186
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      4187..4218
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     primer_bind     1..20
                     /label="dialout_for"
                     /note="Reverse dialout primer. Unique pair for each FreeGenes part."
     primer_bind     2158..2177
                     /label="dialout_rev"
                     /note="Forward dialout primer. Unique pair for each FreeGenes part."
     CDS             29..2143
                     /label="T7 DNA Polymerase (unmodified)"
                     /note=""
                     /translation="MIVSDIEANALLESVTKFHCGVIYDYSTAEYVSYRPSDFGAYLDALEAEVARGGLIVFHNGHKYDVPALTKLAKLQLNREFHLPRENCIDTLVLSRLIHSNLKDTDMGLLRSGKLPGKRFGSHALEAWGYRLGEMKGEYKDDFKRMLEEQGEEYVDGMEWWNFNEEMMDYNVQDVVVTKALLEKLLSDKHYFPPEIDFTDVGYTTFWSESLEAVDIEHRAAWLLAKQERNGFPFDTKAIEELYVELAARRSELLRKLTETFGSWYQPKGGTEMFCHPRTGKPLPKYPRIKTPKVGGIFKKPKNKAQREGREPCELDTREYVAGAPYTPVEHVVFNPSSRDHIQKKLQEAGWVPTKYTDKGAPVVDDEVLEGVRVDDPEKQAAIDLIKEYLMIQKRIGQSAEGDKAWLRYVAEDGKIHGSVNPNGAVTGRATHAFPNLAQIPGVRSPYGEQCRAAFGAEHHLDGITGKPWVQAGIDASGLELRCLAHFMARFDNGEYAHEILNGDIHTKNQIAAELPTRDNAKTFIYGFLYGAGDEKIGQIVGAGKERGKELKKKFLENTPAIAALRESIQQTLVESSQWVAGEQQVKWKRRWIKGLDGRKVHVRSPHAALNTLLQSAGALICKLWIIKTEEMLVEKGLKHGWDGDFAYMAWVHDEIQVGCRTEEIAQVVIETAQEAMRWVGDHWNFRCLLDTEGKMGPNWAICH*"
     primer_bind     complement(2327..2343)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(2410..2998)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(3189..4049)
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
     promoter        complement(4050..4154)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
     misc_feature    2178..2237
                     /label="FreeGenes Barcode"
                     /note="FreeGenes Barcode. Defined as I-SceI site flanked by 21 base pair random sequences."
ORIGIN
        1 AAAGGGGCCG TCAATATCAG GGTCTCTAAT GATCGTATCT GACATCGAGG CGAACGCTCT
       61 GCTGGAATCC GTAACTAAAT TTCACTGCGG TGTCATCTAC GATTATAGCA CCGCAGAGTA
      121 CGTATCGTAC CGCCCGAGCG ACTTCGGTGC TTATCTGGAC GCACTGGAGG CTGAAGTTGC
      181 GCGCGGCGGC CTGATCGTGT TCCATAACGG TCACAAGTAC GATGTGCCGG CTCTGACGAA
      241 ACTGGCGAAA CTCCAGCTGA ACCGTGAGTT TCACCTGCCG CGTGAAAACT GTATCGACAC
      301 CCTGGTCCTG TCCCGCCTCA TCCATAGCAA CCTGAAAGAC ACCGACATGG GCCTGCTGCG
      361 CTCGGGTAAA CTGCCGGGCA AGCGTTTTGG TAGCCACGCT CTCGAAGCGT GGGGTTACCG
      421 TCTGGGCGAA ATGAAAGGTG AGTACAAAGA CGATTTCAAG CGCATGCTGG AAGAACAGGG
      481 TGAAGAATAT GTTGACGGCA TGGAATGGTG GAACTTCAAC GAGGAAATGA TGGATTATAA
      541 CGTGCAGGAT GTGGTTGTGA CTAAAGCACT GCTGGAAAAA CTTCTGTCCG ACAAACACTA
      601 CTTCCCGCCC GAGATCGACT TCACCGACGT AGGTTACACC ACCTTCTGGT CTGAATCCCT
      661 GGAAGCCGTT GATATCGAGC ACCGTGCAGC TTGGCTGTTA GCAAAGCAGG AACGTAATGG
      721 CTTCCCGTTC GATACCAAAG CTATCGAGGA ACTGTACGTA GAGCTGGCTG CGCGTCGTTC
      781 CGAACTGCTG CGTAAGCTGA CTGAAACCTT CGGCTCCTGG TACCAGCCGA AAGGCGGTAC
      841 CGAAATGTTC TGTCATCCGC GTACTGGGAA ACCGCTGCCG AAATATCCGC GTATCAAAAC
      901 GCCGAAAGTA GGCGGGATCT TTAAAAAACC GAAAAACAAA GCTCAGCGCG AAGGCAGAGA
      961 ACCGTGCGAA CTGGACACCC GTGAATACGT TGCTGGCGCA CCGTATACCC CGGTTGAACA
     1021 TGTAGTTTTC AACCCGAGCT CTCGTGACCA CATCCAGAAG AAACTGCAAG AAGCGGGCTG
     1081 GGTGCCGACC AAATATACCG ATAAGGGCGC TCCAGTTGTC GACGACGAAG TTCTGGAAGG
     1141 TGTTCGTGTG GATGACCCGG AAAAACAGGC TGCAATTGAC CTGATCAAAG AATACCTGAT
     1201 GATCCAGAAA CGTATCGGCC AATCGGCAGA AGGCGACAAA GCATGGCTGC GCTATGTTGC
     1261 TGAAGATGGT AAGATCCATG GCTCTGTGAA CCCGAACGGG GCTGTTACCG GCCGTGCCAC
     1321 CCACGCGTTT CCGAACCTGG CGCAGATCCC AGGCGTTCGC TCTCCATACG GTGAACAGTG
     1381 TCGCGCAGCT TTCGGCGCCG AACACCACCT GGACGGCATC ACCGGTAAAC CGTGGGTACA
     1441 GGCCGGTATC GATGCAAGCG GCCTGGAACT GCGTTGTCTG GCACATTTCA TGGCACGTTT
     1501 CGATAACGGT GAGTACGCAC ATGAAATCCT GAACGGTGAT ATTCATACAA AAAACCAGAT
     1561 CGCCGCTGAA CTGCCGACTC GTGACAACGC TAAAACGTTC ATCTACGGCT TTCTGTACGG
     1621 CGCGGGTGAC GAGAAAATTG GCCAGATTGT AGGTGCCGGC AAAGAACGTG GCAAAGAACT
     1681 GAAAAAAAAA TTCCTGGAAA ACACGCCTGC GATCGCGGCT CTGCGCGAAT CAATCCAGCA
     1741 GACTCTGGTT GAGAGCTCGC AGTGGGTTGC CGGTGAACAG CAGGTGAAAT GGAAGCGTCG
     1801 TTGGATTAAA GGTCTGGATG GCCGTAAAGT TCACGTACGC AGCCCGCATG CAGCGTTAAA
     1861 CACCCTCTTG CAATCCGCGG GCGCTCTGAT TTGCAAACTC TGGATCATCA AGACCGAAGA
     1921 AATGCTGGTT GAAAAGGGCC TGAAACACGG CTGGGATGGC GACTTTGCAT ACATGGCATG
     1981 GGTGCATGAC GAGATTCAGG TAGGTTGCCG CACCGAAGAA ATCGCGCAGG TCGTCATTGA
     2041 AACTGCGCAA GAAGCCATGC GTTGGGTGGG TGACCACTGG AACTTCCGTT GCCTCCTGGA
     2101 CACCGAAGGC AAAATGGGCC CTAACTGGGC CATCTGTCAT TGAAGAGCTT TGAGACCAGA
     2161 GAGGCATGAT TGACCTCCGG ATCCTTGTAG GACATCAGTA GGGATAACAG GGTAATGAGC
     2221 CCAGCATTCC AAGCCCGCGC TGAGGTGTCA ATCGTCGGAG CCGCTGAGCA ATAACTAGCA
     2281 TAACCCCTTG GGGCCTCTAA ACGGGTCTTG AGGGGTTTTT TGCATGGTCA TAGCTGTTTC
     2341 CTGAGAGCTT GGCAGGTGAT GACACACATT AACAAATTTC GTGAGGAGTC TCCAGAAGAA
     2401 TGCCATTAAT TTCCATAGGC TCCGCCCCCC TGACGAGCAT CACAAAAATC GACGCTCAAG
     2461 TCAGAGGTGG CGAAACCCGA CAGGACTATA AAGATACCAG GCGTTTCCCC CTGGAAGCTC
     2521 CCTCGTGCGC TCTCCTGTTC CGACCCTGCC GCTTACCGGA TACCTGTCCG CCTTTCTCCC
     2581 TTCGGGAAGC GTGGCGCTTT CTCATAGCTC ACGCTGTAGG TATCTCAGTT CGGTGTAGGT
     2641 CGTTCGCTCC AAGCTGGGCT GTGTGCACGA ACCCCCCGTT CAGCCCGACC GCTGCGCCTT
     2701 ATCCGGTAAC TATCGTCTTG AGTCCAACCC GGTAAGACAC GACTTATCGC CACTGGCAGC
     2761 AGCCACTGGT AACAGGATTA GCAGAGCGAG GTATGTAGGC GGTGCTACAG AGTTCTTGAA
     2821 GTGGTGGCCT AACTACGGCT ACACTAGAAG AACAGTATTT GGTATCTGCG CTCTGCTGAA
     2881 GCCAGTTACC TTCGGAAAAA GAGTTGGTAG CTCTTGATCC GGCAAACAAA CCACCGCTGG
     2941 TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA GATTACGCGC AGAAAAAAAG GATCTCAAGA
     3001 AGGCCTACTA TTAGCAACAA CGATCCTTTG ATCTTTTCTA CGGGGTCTGA CGCTCAGTGG
     3061 AACGAAAACT CACGTTAAGG GATTTTGGTC ATGAGATTAT CAAAAAGGAT CTTCACCTAG
     3121 ATCCTTTTAA ATTAAAAATG AAGTTTTAAA TCAATCTAAA GTATATATGA GTAAACTTGG
     3181 TCTGACAGTT ACCAATGCTT AATCAGTGAG GCACCTATCT CAGCGATCTG TCTATTTCGT
     3241 TCATCCATAG TTGCCTGACT CCCCGTCGTG TAGATAACTA CGATACGGGA GGGCTTACCA
     3301 TCTGGCCCCA GTGCTGCAAT GATACCGCGA GAACCACGCT CACCGGCTCC AGATTTATCA
     3361 GCAATAAACC AGCCAGCCGG AAGGGCCGAG CGCAGAAGTG GTCCTGCAAC TTTATCCGCC
     3421 TCCATCCAGT CTATTAATTG TTGCCGGGAA GCTAGAGTAA GTAGTTCGCC AGTTAATAGT
     3481 TTGCGCAACG TTGTTGCCAT TGCTACAGGC ATCGTGGTGT CACGCTCGTC GTTTGGTATG
     3541 GCTTCATTCA GCTCCGGTTC CCAACGATCA AGGCGAGTTA CATGATCCCC CATGTTGTGC
     3601 AAAAAAGCGG TTAGCTCCTT CGGTCCTCCG ATCGTTGTCA GAAGTAAGTT GGCCGCAGTG
     3661 TTATCACTCA TGGTTATGGC AGCACTGCAT AATTCTCTTA CTGTCATGCC ATCCGTAAGA
     3721 TGCTTTTCTG TGACTGGTGA GTACTCAACC AAGTCATTCT GAGAATAGTG TATGCGGCGA
     3781 CCGAGTTGCT CTTGCCCGGC GTCAATACGG GATAATACCG CGCCACATAG CAGAACTTTA
     3841 AAAGTGCTCA TCATTGGAAA ACGTTCTTCG GGGCGAAAAC TCTCAAGGAT CTTACCGCTG
     3901 TTGAGATCCA GTTCGATGTA ACCCACTCGT GCACCCAACT GATCTTCAGC ATCTTTTACT
     3961 TTCACCAGCG TTTCTGGGTG AGCAAAAACA GGAAGGCAAA ATGCCGCAAA AAAGGGAATA
     4021 AGGGCGACAC GGAAATGTTG AATACTCATA CTCTTCCTTT TTCAATATTA TTGAAGCATT
     4081 TATCAGGGTT ATTGTCTCAT GAGCGGATAC ATATTTGAAT GTATTTAGAA AAATAAACAA
     4141 ATAGGGGTTC CGCGCACCTG CACCAGTCAG TAAAACGACG GCCAGTAGTC AAAAGCCTCC
     4201 GACCGGAGGC TTTTGACTTG GTTCAGGTGG AGTGGGAG
//