LOCUS       Exported     3587 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..3587
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      1624..1671
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     3519..3535
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      3536..3567
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     primer_bind     1..20
                     /label="dialout_for"
                     /note="Reverse dialout primer. Unique pair for each FreeGenes part."
     primer_bind     1507..1526
                     /label="dialout_rev"
                     /note="Forward dialout primer. Unique pair for each FreeGenes part."
     CDS             29..1492
                     /label="T4-DNA Ligase"
                     /note=""
                     /translation="MILKILNEIASIGSTKQKQAILEKNKDNELLKRVYRLTYSRGLQYYIKKWPKPGIATQSFGMLTLTDMLDFIEFTLATRKLTGNAAIEELTGYITDGKKDDVEVLRRVMMRDLECGASVSIANKVWPGLIPEQPQMLASSYDEKGINKNIKFPAFAQLKADGARCFAEVRGDELDDVRLLSRAGNEYLGLDLLKEELIKMTAEARQIHPEGVLIDGELVYHEQVKKEPEGLDFLFDAYPENSKAKEFAEVAESRTASNGIANKSLKGTISEKEAQCMKFQVWDYVPLVEIYSLPAFRLKYDVRFSKLEQMTSGYDKVILIENQVVNNLDEAKVIYKKYIDQGLEGIILKNIDGLWENARSKNLYKFKEVIDVDLKIVGIYPHRKDPTKAGGFILESECGKIKVNAGSGLKDKAGVKSHELDRTRIMENQNYYIGKILECECNGWLKSDGRTDYVKLFLPIAIRLREDKTKANTFEDVFGDFHEVTGL*"
     primer_bind     complement(1676..1692)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(1759..2347)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(2538..3398)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(3399..3503)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
     misc_feature    1527..1586
                     /label="FreeGenes Barcode"
                     /note="FreeGenes Barcode. Defined as I-SceI site flanked by 21 base pair random sequences."
ORIGIN
        1 AAAGGGGCCG TCAATATCAG GGTCTCTAAT GATTCTGAAA ATTCTGAACG AGATTGCCAG
       61 CATCGGCTCT ACCAAGCAGA AACAGGCGAT CCTGGAAAAA AACAAAGATA ACGAGCTGCT
      121 GAAACGTGTT TACCGTCTGA CTTACTCCCG TGGTCTGCAA TACTACATTA AAAAATGGCC
      181 GAAACCGGGT ATTGCCACCC AGTCGTTCGG TATGCTGACC CTGACTGACA TGTTGGATTT
      241 CATCGAGTTC ACCCTTGCTA CTCGTAAACT CACTGGCAAC GCTGCTATCG AGGAACTGAC
      301 TGGCTATATC ACTGACGGCA AAAAAGACGA TGTTGAAGTG CTGCGTCGCG TAATGATGCG
      361 TGATCTCGAA TGCGGTGCCA GCGTTTCCAT CGCCAACAAA GTTTGGCCAG GTCTGATCCC
      421 GGAACAGCCG CAGATGCTGG CATCTAGCTA CGATGAAAAG GGTATTAATA AAAATATTAA
      481 GTTCCCGGCT TTTGCGCAGC TGAAAGCGGA CGGCGCACGC TGTTTTGCTG AAGTACGTGG
      541 CGATGAACTG GATGACGTGC GTCTGCTGTC ACGTGCAGGT AATGAATACC TGGGCCTGGA
      601 TCTGCTGAAA GAAGAACTGA TCAAAATGAC TGCCGAAGCA CGTCAGATCC ACCCGGAAGG
      661 TGTTCTGATC GATGGCGAAC TCGTTTATCA CGAACAAGTG AAAAAAGAAC CAGAAGGTCT
      721 GGATTTCCTG TTCGATGCGT ATCCGGAAAA CTCCAAGGCA AAAGAGTTCG CGGAAGTGGC
      781 TGAATCCCGT ACTGCAAGCA ACGGCATTGC AAACAAATCC CTCAAAGGGA CTATCAGCGA
      841 AAAGGAAGCA CAGTGCATGA AGTTCCAGGT ATGGGATTAC GTACCGCTGG TTGAAATCTA
      901 CTCTCTGCCG GCGTTCCGTC TGAAATACGA CGTGCGCTTC TCTAAACTGG AACAGATGAC
      961 CAGCGGTTAC GACAAGGTTA TCCTGATCGA GAACCAGGTG GTTAACAACC TCGATGAGGC
     1021 TAAAGTAATT TATAAGAAAT ACATTGACCA GGGTCTGGAA GGTATCATCC TGAAAAACAT
     1081 CGATGGCCTG TGGGAGAACG CGCGTAGCAA GAACTTGTAC AAATTTAAAG AGGTAATCGA
     1141 CGTTGACCTG AAAATTGTTG GTATCTACCC ACACCGCAAG GACCCGACCA AAGCGGGCGG
     1201 CTTCATCCTG GAATCTGAGT GCGGTAAAAT CAAAGTCAAC GCGGGTAGCG GTCTGAAAGA
     1261 CAAAGCAGGT GTAAAATCTC ACGAACTGGA TCGTACCCGT ATTATGGAAA ACCAGAACTA
     1321 TTACATTGGC AAAATTCTGG AATGTGAATG CAACGGTTGG CTGAAATCTG ACGGTCGCAC
     1381 CGATTATGTG AAACTTTTTC TGCCGATTGC CATTCGCCTG CGCGAAGATA AGACCAAAGC
     1441 AAACACCTTC GAAGATGTGT TCGGTGATTT TCACGAAGTT ACCGGTCTGT GAAGAGCTTT
     1501 GAGACCAGAT AGATGCTCCG TCAAGCCTTC TGCTCCGTTT GTATGCATAG GGATAACAGG
     1561 GTAATCCTCG CTATTTTGAC TAGCAGCGCT GAGGTGTCAA TCGTCGGAGC CGCTGAGCAA
     1621 TAACTAGCAT AACCCCTTGG GGCCTCTAAA CGGGTCTTGA GGGGTTTTTT GCATGGTCAT
     1681 AGCTGTTTCC TGAGAGCTTG GCAGGTGATG ACACACATTA ACAAATTTCG TGAGGAGTCT
     1741 CCAGAAGAAT GCCATTAATT TCCATAGGCT CCGCCCCCCT GACGAGCATC ACAAAAATCG
     1801 ACGCTCAAGT CAGAGGTGGC GAAACCCGAC AGGACTATAA AGATACCAGG CGTTTCCCCC
     1861 TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC GACCCTGCCG CTTACCGGAT ACCTGTCCGC
     1921 CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC TCATAGCTCA CGCTGTAGGT ATCTCAGTTC
     1981 GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG TGTGCACGAA CCCCCCGTTC AGCCCGACCG
     2041 CTGCGCCTTA TCCGGTAACT ATCGTCTTGA GTCCAACCCG GTAAGACACG ACTTATCGCC
     2101 ACTGGCAGCA GCCACTGGTA ACAGGATTAG CAGAGCGAGG TATGTAGGCG GTGCTACAGA
     2161 GTTCTTGAAG TGGTGGCCTA ACTACGGCTA CACTAGAAGA ACAGTATTTG GTATCTGCGC
     2221 TCTGCTGAAG CCAGTTACCT TCGGAAAAAG AGTTGGTAGC TCTTGATCCG GCAAACAAAC
     2281 CACCGCTGGT AGCGGTGGTT TTTTTGTTTG CAAGCAGCAG ATTACGCGCA GAAAAAAAGG
     2341 ATCTCAAGAA GGCCTACTAT TAGCAACAAC GATCCTTTGA TCTTTTCTAC GGGGTCTGAC
     2401 GCTCAGTGGA ACGAAAACTC ACGTTAAGGG ATTTTGGTCA TGAGATTATC AAAAAGGATC
     2461 TTCACCTAGA TCCTTTTAAA TTAAAAATGA AGTTTTAAAT CAATCTAAAG TATATATGAG
     2521 TAAACTTGGT CTGACAGTTA CCAATGCTTA ATCAGTGAGG CACCTATCTC AGCGATCTGT
     2581 CTATTTCGTT CATCCATAGT TGCCTGACTC CCCGTCGTGT AGATAACTAC GATACGGGAG
     2641 GGCTTACCAT CTGGCCCCAG TGCTGCAATG ATACCGCGAG AACCACGCTC ACCGGCTCCA
     2701 GATTTATCAG CAATAAACCA GCCAGCCGGA AGGGCCGAGC GCAGAAGTGG TCCTGCAACT
     2761 TTATCCGCCT CCATCCAGTC TATTAATTGT TGCCGGGAAG CTAGAGTAAG TAGTTCGCCA
     2821 GTTAATAGTT TGCGCAACGT TGTTGCCATT GCTACAGGCA TCGTGGTGTC ACGCTCGTCG
     2881 TTTGGTATGG CTTCATTCAG CTCCGGTTCC CAACGATCAA GGCGAGTTAC ATGATCCCCC
     2941 ATGTTGTGCA AAAAAGCGGT TAGCTCCTTC GGTCCTCCGA TCGTTGTCAG AAGTAAGTTG
     3001 GCCGCAGTGT TATCACTCAT GGTTATGGCA GCACTGCATA ATTCTCTTAC TGTCATGCCA
     3061 TCCGTAAGAT GCTTTTCTGT GACTGGTGAG TACTCAACCA AGTCATTCTG AGAATAGTGT
     3121 ATGCGGCGAC CGAGTTGCTC TTGCCCGGCG TCAATACGGG ATAATACCGC GCCACATAGC
     3181 AGAACTTTAA AAGTGCTCAT CATTGGAAAA CGTTCTTCGG GGCGAAAACT CTCAAGGATC
     3241 TTACCGCTGT TGAGATCCAG TTCGATGTAA CCCACTCGTG CACCCAACTG ATCTTCAGCA
     3301 TCTTTTACTT TCACCAGCGT TTCTGGGTGA GCAAAAACAG GAAGGCAAAA TGCCGCAAAA
     3361 AAGGGAATAA GGGCGACACG GAAATGTTGA ATACTCATAC TCTTCCTTTT TCAATATTAT
     3421 TGAAGCATTT ATCAGGGTTA TTGTCTCATG AGCGGATACA TATTTGAATG TATTTAGAAA
     3481 AATAAACAAA TAGGGGTTCC GCGCACCTGC ACCAGTCAGT AAAACGACGG CCAGTAGTCA
     3541 AAAGCCTCCG ACCGGAGGCT TTTGACTTGG TTCAGGTGGA GTGGGAG
//