LOCUS       Exported     3803 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..3803
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      1840..1887
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     3735..3751
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      3752..3783
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     primer_bind     1..20
                     /label="dialout_for"
                     /note="Reverse dialout primer. Unique pair for each FreeGenes part."
     primer_bind     1723..1742
                     /label="dialout_rev"
                     /note="Forward dialout primer. Unique pair for each FreeGenes part."
     CDS             29..1708
                     /label="9 Degrees North DNA Ligase"
                     /note=""
                     /translation="MKYSELAELYRRLEKTTLKTLKTKFVADFLKKTPDDLLEIVPYLILGKVFPDWDERELGVGEKLLIRAVSMATGVPEREIENSIKDTGDLGESVALALKKRKQKSFFSQPLTIKRVYSTFVKVAEASGEGSQDRKMKYLANLFMDAQPEEGKYIARTVLGTMRTGVAEGILRDAIAEAFKVKPELVERAYMLTSDFGYVAKVAKLEGNEGLSKVSIQIGKPIRPMLAQNAANVKEALIEMGGEAAFEIKYDGARVQVHRDGDRVIIYSRRLENVTRSIPEIVEAVKASLKPSKVIVEGELVAVGENGRPRPFQYVLRRFRRKYNIEEMIEKIPLELNLFDILYVDGESLIDTKFAERRKKLEESVEESDKIKLAEQLVTKKVEEAEEFYKRALELGHEGLMAKRLDSIYEPGNRGKKWLKIKPTMENLDLVIIGAEWGEGRRAHLLGSFLVAAYDPESGEFVPVGKVGSGFTDEDLVEFTKMLKPLIVREEGKFVEIEPKVVIEVTYQEIQKSPKYKSGFALRFPRYVALREDKSPEEADTIERVAQLYELQERFKAKR*"
     primer_bind     complement(1892..1908)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(1975..2563)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(2754..3614)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(3615..3719)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
     misc_feature    1743..1802
                     /label="FreeGenes Barcode"
                     /note="FreeGenes Barcode. Defined as I-SceI site flanked by 21 base pair random sequences."
ORIGIN
        1 AAAGGGGCCG TCAATATCAG GGTCTCTAAT GAAATACTCC GAACTGGCTG AACTGTATCG
       61 TCGCCTGGAA AAAACCACCC TGAAAACCTT GAAAACCAAA TTCGTGGCGG ACTTCCTGAA
      121 AAAAACCCCG GACGACCTCC TGGAAATCGT GCCGTACCTG ATCCTCGGCA AAGTATTCCC
      181 AGACTGGGAC GAGCGTGAGC TGGGTGTTGG TGAAAAACTG CTGATCCGTG CTGTATCCAT
      241 GGCAACTGGC GTGCCGGAGC GTGAAATCGA GAACTCTATT AAAGATACCG GTGACCTGGG
      301 CGAATCCGTG GCCCTGGCGC TGAAAAAACG CAAACAGAAA TCTTTCTTCT CTCAACCGCT
      361 GACGATCAAA CGTGTCTACT CAACCTTCGT TAAAGTTGCG GAAGCATCTG GTGAAGGCTC
      421 CCAGGATCGT AAAATGAAAT ATCTGGCCAA CTTGTTTATG GACGCCCAGC CGGAGGAAGG
      481 CAAATACATT GCTCGTACCG TTCTGGGCAC CATGCGCACG GGTGTGGCTG AAGGCATCCT
      541 GCGTGACGCG ATCGCAGAAG CTTTCAAAGT CAAGCCGGAA CTGGTAGAAC GCGCGTACAT
      601 GCTGACCTCT GACTTCGGCT ACGTCGCAAA AGTTGCGAAA CTCGAAGGTA ACGAAGGTCT
      661 GTCGAAAGTT TCTATTCAGA TCGGCAAACC GATTCGTCCT ATGCTGGCGC AAAACGCCGC
      721 AAACGTAAAA GAAGCGCTGA TTGAAATGGG CGGAGAAGCG GCTTTCGAAA TTAAGTACGA
      781 CGGTGCTCGC GTTCAGGTAC ATCGTGACGG CGATCGTGTT ATCATTTATA GCCGTCGTCT
      841 GGAGAACGTT ACCCGTTCCA TCCCGGAAAT CGTTGAGGCG GTTAAAGCTT CGCTGAAACC
      901 GTCTAAAGTT ATCGTGGAAG GCGAGCTGGT GGCAGTCGGT GAAAACGGCC GTCCGCGTCC
      961 GTTCCAGTAC GTTCTGCGCC GTTTCCGTCG CAAGTACAAC ATCGAGGAAA TGATTGAAAA
     1021 AATTCCGTTG GAACTGAACT TGTTCGATAT CCTGTACGTG GACGGCGAAT CCCTTATCGA
     1081 CACAAAATTC GCTGAACGCC GTAAAAAACT GGAAGAATCT GTTGAAGAAT CAGACAAAAT
     1141 CAAGCTGGCC GAGCAGCTGG TCACTAAAAA AGTGGAAGAA GCGGAAGAGT TCTACAAACG
     1201 CGCTCTGGAG CTGGGTCACG AAGGCCTGAT GGCAAAACGT CTGGATTCTA TTTACGAACC
     1261 GGGCAACCGC GGTAAAAAAT GGCTGAAAAT TAAACCGACT ATGGAAAACC TGGATTTAGT
     1321 TATTATCGGT GCAGAATGGG GCGAGGGTCG CCGTGCGCAT CTCCTGGGTT CTTTCCTGGT
     1381 TGCGGCTTAC GACCCGGAAT CTGGCGAATT TGTACCGGTA GGTAAAGTTG GCTCTGGTTT
     1441 CACGGATGAG GACCTGGTTG AGTTCACTAA AATGCTGAAA CCGCTGATCG TTCGTGAAGA
     1501 AGGTAAATTC GTGGAAATCG AACCGAAAGT TGTTATCGAA GTAACCTATC AGGAAATTCA
     1561 AAAATCTCCT AAATATAAGA GCGGTTTCGC CCTGCGTTTC CCGCGCTACG TGGCTCTCCG
     1621 TGAAGATAAA AGCCCGGAAG AAGCGGACAC TATCGAACGT GTGGCACAGC TGTACGAGCT
     1681 GCAAGAACGC TTCAAAGCAA AACGCTGAAG AGCTTTGAGA CCAGATTGTT ACTCCGACGG
     1741 ACTGATCCTG TTTGTTAAGT AGGTAGGGAT AACAGGGTAA TTTTTACATG ACTCTTCCGT
     1801 TCCGCTGAGG TGTCAATCGT CGGAGCCGCT GAGCAATAAC TAGCATAACC CCTTGGGGCC
     1861 TCTAAACGGG TCTTGAGGGG TTTTTTGCAT GGTCATAGCT GTTTCCTGAG AGCTTGGCAG
     1921 GTGATGACAC ACATTAACAA ATTTCGTGAG GAGTCTCCAG AAGAATGCCA TTAATTTCCA
     1981 TAGGCTCCGC CCCCCTGACG AGCATCACAA AAATCGACGC TCAAGTCAGA GGTGGCGAAA
     2041 CCCGACAGGA CTATAAAGAT ACCAGGCGTT TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC
     2101 TGTTCCGACC CTGCCGCTTA CCGGATACCT GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC
     2161 GCTTTCTCAT AGCTCACGCT GTAGGTATCT CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT
     2221 GGGCTGTGTG CACGAACCCC CCGTTCAGCC CGACCGCTGC GCCTTATCCG GTAACTATCG
     2281 TCTTGAGTCC AACCCGGTAA GACACGACTT ATCGCCACTG GCAGCAGCCA CTGGTAACAG
     2341 GATTAGCAGA GCGAGGTATG TAGGCGGTGC TACAGAGTTC TTGAAGTGGT GGCCTAACTA
     2401 CGGCTACACT AGAAGAACAG TATTTGGTAT CTGCGCTCTG CTGAAGCCAG TTACCTTCGG
     2461 AAAAAGAGTT GGTAGCTCTT GATCCGGCAA ACAAACCACC GCTGGTAGCG GTGGTTTTTT
     2521 TGTTTGCAAG CAGCAGATTA CGCGCAGAAA AAAAGGATCT CAAGAAGGCC TACTATTAGC
     2581 AACAACGATC CTTTGATCTT TTCTACGGGG TCTGACGCTC AGTGGAACGA AAACTCACGT
     2641 TAAGGGATTT TGGTCATGAG ATTATCAAAA AGGATCTTCA CCTAGATCCT TTTAAATTAA
     2701 AAATGAAGTT TTAAATCAAT CTAAAGTATA TATGAGTAAA CTTGGTCTGA CAGTTACCAA
     2761 TGCTTAATCA GTGAGGCACC TATCTCAGCG ATCTGTCTAT TTCGTTCATC CATAGTTGCC
     2821 TGACTCCCCG TCGTGTAGAT AACTACGATA CGGGAGGGCT TACCATCTGG CCCCAGTGCT
     2881 GCAATGATAC CGCGAGAACC ACGCTCACCG GCTCCAGATT TATCAGCAAT AAACCAGCCA
     2941 GCCGGAAGGG CCGAGCGCAG AAGTGGTCCT GCAACTTTAT CCGCCTCCAT CCAGTCTATT
     3001 AATTGTTGCC GGGAAGCTAG AGTAAGTAGT TCGCCAGTTA ATAGTTTGCG CAACGTTGTT
     3061 GCCATTGCTA CAGGCATCGT GGTGTCACGC TCGTCGTTTG GTATGGCTTC ATTCAGCTCC
     3121 GGTTCCCAAC GATCAAGGCG AGTTACATGA TCCCCCATGT TGTGCAAAAA AGCGGTTAGC
     3181 TCCTTCGGTC CTCCGATCGT TGTCAGAAGT AAGTTGGCCG CAGTGTTATC ACTCATGGTT
     3241 ATGGCAGCAC TGCATAATTC TCTTACTGTC ATGCCATCCG TAAGATGCTT TTCTGTGACT
     3301 GGTGAGTACT CAACCAAGTC ATTCTGAGAA TAGTGTATGC GGCGACCGAG TTGCTCTTGC
     3361 CCGGCGTCAA TACGGGATAA TACCGCGCCA CATAGCAGAA CTTTAAAAGT GCTCATCATT
     3421 GGAAAACGTT CTTCGGGGCG AAAACTCTCA AGGATCTTAC CGCTGTTGAG ATCCAGTTCG
     3481 ATGTAACCCA CTCGTGCACC CAACTGATCT TCAGCATCTT TTACTTTCAC CAGCGTTTCT
     3541 GGGTGAGCAA AAACAGGAAG GCAAAATGCC GCAAAAAAGG GAATAAGGGC GACACGGAAA
     3601 TGTTGAATAC TCATACTCTT CCTTTTTCAA TATTATTGAA GCATTTATCA GGGTTATTGT
     3661 CTCATGAGCG GATACATATT TGAATGTATT TAGAAAAATA AACAAATAGG GGTTCCGCGC
     3721 ACCTGCACCA GTCAGTAAAA CGACGGCCAG TAGTCAAAAG CCTCCGACCG GAGGCTTTTG
     3781 ACTTGGTTCA GGTGGAGTGG GAG
//