LOCUS       Exported     3809 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..3809
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      1846..1893
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     3741..3757
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      3758..3789
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     primer_bind     1..20
                     /note="Reverse dialout primer. Unique pair for each FreeGenes part."
                     /label="dialout_for"
     primer_bind     1729..1748
                     /label="dialout_rev"
                     /note="Forward dialout primer. Unique pair for each FreeGenes part."
     CDS             29..1714
                     /label="Pfu DNA Ligase"
                     /note=""
                     /translation="MRYLELAQLYQKLEKTTMKLIKTRLVADFLKKVPDDHLEFIPYLILGEVFPEWDERELGVGEKLLIKAVAMATGIDAKEIEESVKDTGDLGESIALAVKKKKQKSFFSQPLTIKRVYQTLVKVAETTGEGSQDKKVKYLADLFMDAEPLEAKYLARTILGTMRTGVAEGLLRDAIAMAFHVKVELVERAYMLTSDFGYVAKIAKLEGNEGLAKVQVQLGKPIKPMLAQQAASIRDALLEMGGEAEFEIKYDGARVQVHKDGSKIIVYSRRLENVTRAIPEIVEALKEAIIPEKAIVEGELVAIGENGRPLPFQYVLRRFRRKHNIEEMMEKIPLELNLFDVLYVDGQSLIDTKFIDRRRTLEEIIKQNEKIKVAENLITKKVEEAEAFYKRALEMGHEGLMAKRLDAVYEPGNRGKKWLKIKPTMENLDLVIIGAEWGEGRRAHLFGSFILGAYDPETGEFLEVGKVGSGFTDDDLVEFTKMLKPLIIKEEGKRVWLQPKVVIEVTYQEIQKSPKYRSGFALRFPRFVALRDDKGPEDADTIERIAQLYELQEKMKGKVES*"
     primer_bind     complement(1898..1914)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(1981..2569)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(2760..3620)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(3621..3725)
                     /note="ampicillin resistance gene promoter"
                     /label="bla promoter"
     misc_feature    1749..1808
                     /label="FreeGenes Barcode"
                     /note="FreeGenes Barcode. Defined as I-SceI site flanked by 21 base pair random sequences."
ORIGIN
        1 AAAGGGGCCG TCAATATCAG GGTCTCTAAT GCGCTACCTG GAGCTGGCAC AGCTGTATCA
       61 GAAACTGGAA AAGACTACCA TGAAACTGAT TAAGACCCGC CTGGTGGCAG ACTTCCTGAA
      121 AAAAGTACCG GACGATCATC TCGAATTTAT CCCGTACCTG ATCCTGGGCG AGGTTTTTCC
      181 GGAATGGGAC GAACGCGAAC TGGGCGTTGG TGAGAAACTG CTGATCAAAG CCGTGGCTAT
      241 GGCAACCGGT ATCGACGCGA AAGAGATCGA AGAAAGCGTA AAAGACACCG GCGACCTAGG
      301 CGAGAGCATT GCGCTGGCGG TTAAAAAGAA AAAACAGAAA TCTTTCTTCT CTCAGCCGTT
      361 GACCATCAAA CGTGTATATC AGACCCTGGT GAAAGTCGCG GAAACCACTG GTGAAGGCTC
      421 CCAGGACAAG AAAGTGAAAT ACCTTGCTGA CCTTTTCATG GATGCGGAAC CACTGGAAGC
      481 GAAATATCTG GCCCGTACCA TCCTGGGCAC TATGCGTACC GGTGTAGCGG AAGGTCTGCT
      541 GCGTGACGCT ATCGCTATGG CGTTTCACGT AAAGGTTGAA CTCGTCGAAC GCGCGTATAT
      601 GCTGACCTCT GACTTCGGCT ATGTGGCTAA AATTGCCAAG CTGGAAGGTA ACGAAGGTCT
      661 GGCGAAAGTA CAGGTTCAAC TGGGAAAGCC GATCAAACCG ATGCTGGCAC AGCAGGCCGC
      721 AAGCATCCGC GACGCTTTAC TGGAAATGGG GGGTGAGGCT GAGTTTGAAA TCAAATATGA
      781 CGGTGCGCGT GTTCAGGTTC ACAAAGACGG TTCCAAAATC ATCGTTTACT CCCGTCGCCT
      841 GGAAAACGTT ACTCGCGCAA TCCCGGAAAT CGTTGAGGCA TTAAAAGAAG CTATTATTCC
      901 GGAAAAAGCT ATTGTTGAAG GTGAACTGGT GGCTATCGGC GAAAACGGCC GTCCGCTCCC
      961 GTTTCAGTAT GTACTGCGTC GCTTCCGCCG TAAACATAAC ATCGAAGAAA TGATGGAAAA
     1021 AATCCCGCTG GAACTCAATC TGTTTGATGT TCTGTACGTA GATGGTCAGT CACTGATCGA
     1081 TACTAAATTC ATTGACCGTC GTCGTACTTT AGAAGAAATC ATCAAGCAGA ACGAAAAAAT
     1141 CAAAGTGGCC GAAAATCTGA TCACCAAAAA AGTGGAAGAA GCCGAAGCGT TCTACAAACG
     1201 CGCTCTGGAA ATGGGTCATG AAGGCCTGAT GGCAAAACGC CTGGATGCTG TTTACGAACC
     1261 GGGTAACCGT GGTAAGAAGT GGCTGAAGAT CAAACCGACC ATGGAAAACC TCGATCTGGT
     1321 TATTATTGGC GCTGAATGGG GTGAAGGTCG CCGTGCCCAC CTGTTCGGCT CTTTTATCCT
     1381 GGGCGCTTAC GACCCAGAAA CTGGTGAGTT CCTGGAAGTG GGGAAAGTTG GCTCCGGCTT
     1441 TACCGACGAC GATCTGGTTG AGTTCACCAA AATGCTGAAA CCGCTGATTA TTAAAGAAGA
     1501 AGGCAAGCGT GTGTGGCTGC AACCGAAGGT CGTGATTGAA GTTACCTACC AGGAAATCCA
     1561 GAAAAGCCCG AAATACCGTT CAGGCTTCGC TCTGCGTTTT CCGCGTTTCG TTGCTCTGCG
     1621 TGACGATAAA GGCCCGGAGG ACGCCGACAC CATCGAACGT ATTGCCCAAC TGTACGAGCT
     1681 GCAAGAGAAG ATGAAAGGTA AAGTTGAATC CTGAAGAGCT TTGAGACCAG ATTTCCGACG
     1741 AGATTCCCCT TCCGATCTTC TTGCCTTATT AGGGATAACA GGGTAATCGC ACGAAGGGTA
     1801 TACGCAGGCG CTGAGGTGTC AATCGTCGGA GCCGCTGAGC AATAACTAGC ATAACCCCTT
     1861 GGGGCCTCTA AACGGGTCTT GAGGGGTTTT TTGCATGGTC ATAGCTGTTT CCTGAGAGCT
     1921 TGGCAGGTGA TGACACACAT TAACAAATTT CGTGAGGAGT CTCCAGAAGA ATGCCATTAA
     1981 TTTCCATAGG CTCCGCCCCC CTGACGAGCA TCACAAAAAT CGACGCTCAA GTCAGAGGTG
     2041 GCGAAACCCG ACAGGACTAT AAAGATACCA GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG
     2101 CTCTCCTGTT CCGACCCTGC CGCTTACCGG ATACCTGTCC GCCTTTCTCC CTTCGGGAAG
     2161 CGTGGCGCTT TCTCATAGCT CACGCTGTAG GTATCTCAGT TCGGTGTAGG TCGTTCGCTC
     2221 CAAGCTGGGC TGTGTGCACG AACCCCCCGT TCAGCCCGAC CGCTGCGCCT TATCCGGTAA
     2281 CTATCGTCTT GAGTCCAACC CGGTAAGACA CGACTTATCG CCACTGGCAG CAGCCACTGG
     2341 TAACAGGATT AGCAGAGCGA GGTATGTAGG CGGTGCTACA GAGTTCTTGA AGTGGTGGCC
     2401 TAACTACGGC TACACTAGAA GAACAGTATT TGGTATCTGC GCTCTGCTGA AGCCAGTTAC
     2461 CTTCGGAAAA AGAGTTGGTA GCTCTTGATC CGGCAAACAA ACCACCGCTG GTAGCGGTGG
     2521 TTTTTTTGTT TGCAAGCAGC AGATTACGCG CAGAAAAAAA GGATCTCAAG AAGGCCTACT
     2581 ATTAGCAACA ACGATCCTTT GATCTTTTCT ACGGGGTCTG ACGCTCAGTG GAACGAAAAC
     2641 TCACGTTAAG GGATTTTGGT CATGAGATTA TCAAAAAGGA TCTTCACCTA GATCCTTTTA
     2701 AATTAAAAAT GAAGTTTTAA ATCAATCTAA AGTATATATG AGTAAACTTG GTCTGACAGT
     2761 TACCAATGCT TAATCAGTGA GGCACCTATC TCAGCGATCT GTCTATTTCG TTCATCCATA
     2821 GTTGCCTGAC TCCCCGTCGT GTAGATAACT ACGATACGGG AGGGCTTACC ATCTGGCCCC
     2881 AGTGCTGCAA TGATACCGCG AGAACCACGC TCACCGGCTC CAGATTTATC AGCAATAAAC
     2941 CAGCCAGCCG GAAGGGCCGA GCGCAGAAGT GGTCCTGCAA CTTTATCCGC CTCCATCCAG
     3001 TCTATTAATT GTTGCCGGGA AGCTAGAGTA AGTAGTTCGC CAGTTAATAG TTTGCGCAAC
     3061 GTTGTTGCCA TTGCTACAGG CATCGTGGTG TCACGCTCGT CGTTTGGTAT GGCTTCATTC
     3121 AGCTCCGGTT CCCAACGATC AAGGCGAGTT ACATGATCCC CCATGTTGTG CAAAAAAGCG
     3181 GTTAGCTCCT TCGGTCCTCC GATCGTTGTC AGAAGTAAGT TGGCCGCAGT GTTATCACTC
     3241 ATGGTTATGG CAGCACTGCA TAATTCTCTT ACTGTCATGC CATCCGTAAG ATGCTTTTCT
     3301 GTGACTGGTG AGTACTCAAC CAAGTCATTC TGAGAATAGT GTATGCGGCG ACCGAGTTGC
     3361 TCTTGCCCGG CGTCAATACG GGATAATACC GCGCCACATA GCAGAACTTT AAAAGTGCTC
     3421 ATCATTGGAA AACGTTCTTC GGGGCGAAAA CTCTCAAGGA TCTTACCGCT GTTGAGATCC
     3481 AGTTCGATGT AACCCACTCG TGCACCCAAC TGATCTTCAG CATCTTTTAC TTTCACCAGC
     3541 GTTTCTGGGT GAGCAAAAAC AGGAAGGCAA AATGCCGCAA AAAAGGGAAT AAGGGCGACA
     3601 CGGAAATGTT GAATACTCAT ACTCTTCCTT TTTCAATATT ATTGAAGCAT TTATCAGGGT
     3661 TATTGTCTCA TGAGCGGATA CATATTTGAA TGTATTTAGA AAAATAAACA AATAGGGGTT
     3721 CCGCGCACCT GCACCAGTCA GTAAAACGAC GGCCAGTAGT CAAAAGCCTC CGACCGGAGG
     3781 CTTTTGACTT GGTTCAGGTG GAGTGGGAG
//