LOCUS       Exported     4148 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..4148
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      2185..2232
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
                     /label="T7 terminator"
     primer_bind     4080..4096
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      4097..4128
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     primer_bind     1..20
                     /label="dialout_for"
                     /note="Reverse dialout primer. Unique pair for each FreeGenes part."
     primer_bind     2068..2087
                     /label="dialout_rev"
                     /note="Forward dialout primer. Unique pair for each FreeGenes part."
     CDS             29..2053
                     /label="Taq DNA Ligase"
                     /note=""
                     /translation="MTLEAARRRVNELRDLIRYHNYLYYVLDAPEISDAEYDRLLRELKELEERFPELQSPDSPTEQVGARPLESTFRPVRHPTRMYSLDNAFSLDEVRAFEERIERALGRKGPFLYTVEHKVDGLSVNLYYEEGILVFGATRGDGETGEEVTQNLLTIRTIPRRLTGVPDRLEVRGEVYMPIEAFLRLNQELEEAGERIFKNPRNAAAGSLRQKDPRVTARRGLRATFYALGLGLEETGLKSQHDLLLWLRERGFPVEHGFTRALGAEGVEEVYQAWLKERRKLPFEADGVVVKLDDLALWRELGYTARAPRFALAYKFPAEEKETRLLSVAFQVGRTGRITPVGVLEPVFIEGSEVSRVTLHNESFIEELDVRIGDWVLVHKAGGVIPEVLRVLKERRTGEEKPILWPENCPECGHALIKEGKVHRCPNPLCPAKRFEAIRHYASRKAMDIQGLGEKLIEKLLEKGLVRDVADLYRLKKEDLVNLERMGEKSAENLLRQIEESKGRGLERLLYALGLPGVGEVLARNLALRFGHMDRLLEAGLEDLLEVEGVGELTARAILNTLKDPEFRDLVRRLKEAGVEMEAKEREGEALKGLTFVITGELSRPREEVKALLRRLGAKVTDSVSRKTGFLVVGENPGSKLEKARALGVPTLSEEELYRLIEERTGKDPRALTA*"
     primer_bind     complement(2237..2253)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(2320..2908)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(3099..3959)
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
                     /label="bla"
     promoter        complement(3960..4064)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
     misc_feature    2088..2147
                     /label="FreeGenes Barcode"
                     /note="FreeGenes Barcode. Defined as I-SceI site flanked by 21 base pair random sequences."
ORIGIN
        1 AAAGGGGCCG TCAATATCAG GGTCTCTAAT GACACTGGAA GCGGCCCGTC GCCGTGTGAA
       61 CGAGCTGCGC GATCTGATCC GCTATCATAA CTACTTATAC TATGTCCTGG ATGCGCCGGA
      121 AATTTCCGAC GCAGAATATG ATCGTCTGTT GCGTGAACTG AAAGAACTGG AAGAACGCTT
      181 TCCAGAACTC CAATCTCCGG ACTCGCCGAC TGAACAGGTC GGCGCTCGTC CTCTGGAAAG
      241 CACCTTTCGT CCCGTACGTC ACCCGACCCG TATGTACTCC CTGGACAACG CATTCTCCCT
      301 TGACGAAGTG CGTGCCTTTG AAGAACGTAT CGAACGCGCA CTGGGTCGTA AAGGCCCGTT
      361 CCTGTACACC GTTGAACACA AAGTGGACGG TCTTTCCGTG AACCTGTATT ACGAAGAAGG
      421 TATCCTGGTT TTCGGTGCAA CCCGCGGTGA TGGTGAAACA GGCGAAGAAG TTACCCAGAA
      481 TCTGCTGACC ATCCGTACCA TTCCACGCCG TCTGACCGGT GTTCCTGATC GTCTGGAAGT
      541 ACGCGGCGAA GTGTACATGC CAATCGAAGC CTTCCTGCGC CTGAACCAAG AGCTGGAAGA
      601 AGCGGGCGAA CGTATCTTTA AAAACCCGCG CAACGCGGCA GCAGGCTCTC TGCGCCAGAA
      661 GGACCCGCGT GTGACTGCGC GCCGCGGTCT GCGTGCGACT TTCTACGCGT TGGGTCTGGG
      721 CCTTGAAGAA ACCGGCCTGA AATCTCAGCA CGATCTGCTG CTGTGGCTGC GTGAGCGTGG
      781 TTTTCCGGTT GAACACGGTT TTACCCGTGC CCTGGGCGCA GAGGGTGTTG AAGAAGTTTA
      841 TCAGGCCTGG CTGAAGGAGC GTCGTAAACT CCCGTTCGAG GCAGACGGTG TGGTGGTAAA
      901 ACTGGATGAC CTGGCTCTTT GGCGTGAACT TGGTTACACC GCCCGTGCTC CGCGTTTTGC
      961 TCTGGCGTAC AAATTCCCGG CGGAAGAGAA AGAAACTCGT CTGCTGTCCG TTGCGTTCCA
     1021 GGTGGGTCGT ACTGGTCGCA TCACCCCAGT AGGCGTGCTG GAGCCGGTAT TCATCGAGGG
     1081 CAGCGAAGTG TCTCGTGTAA CCCTGCACAA CGAATCCTTC ATCGAAGAAC TTGATGTGCG
     1141 TATCGGCGAT TGGGTACTGG TGCACAAAGC AGGAGGTGTG ATCCCGGAAG TCCTCCGTGT
     1201 GCTGAAAGAA CGTCGCACTG GTGAGGAAAA ACCAATCCTG TGGCCGGAAA ACTGCCCGGA
     1261 ATGTGGCCAC GCTCTGATCA AAGAAGGGAA AGTGCACCGT TGTCCGAACC CACTGTGTCC
     1321 GGCTAAACGT TTCGAAGCTA TTCGCCACTA CGCCAGCCGT AAGGCGATGG ACATTCAGGG
     1381 CCTGGGTGAA AAACTGATTG AAAAACTGCT GGAAAAAGGC CTGGTACGTG ACGTGGCTGA
     1441 CCTGTACCGT CTGAAAAAAG AGGACCTGGT AAACCTGGAG CGAATGGGTG AAAAGTCGGC
     1501 GGAAAACCTG CTCCGTCAAA TCGAAGAAAG CAAAGGCCGT GGCCTGGAGC GTCTGCTGTA
     1561 TGCGCTGGGC CTGCCGGGCG TAGGCGAAGT TCTGGCTCGC AACCTGGCGT TGCGCTTCGG
     1621 TCACATGGAC CGTCTGCTGG AGGCTGGTCT GGAGGACCTG CTGGAAGTGG AGGGTGTAGG
     1681 CGAACTCACG GCCCGTGCCA TCCTCAACAC CCTGAAAGAC CCAGAGTTCC GTGATCTGGT
     1741 ACGTCGTCTG AAAGAAGCCG GTGTCGAAAT GGAAGCCAAA GAACGCGAAG GTGAAGCCCT
     1801 GAAAGGCCTG ACCTTCGTGA TTACTGGTGA GCTGTCTCGT CCGCGTGAAG AAGTGAAAGC
     1861 ACTGCTGCGC CGCCTGGGCG CGAAGGTGAC TGACTCCGTA TCTCGTAAAA CTGGTTTCCT
     1921 GGTTGTTGGT GAAAACCCGG GTTCTAAACT GGAAAAAGCA CGTGCGCTTG GTGTGCCTAC
     1981 CCTGTCGGAG GAAGAACTGT ATCGTTTAAT CGAAGAACGT ACCGGTAAAG ATCCGCGTGC
     2041 GCTGACTGCC TGAAGAGCTT TGAGACCAGC ATCCGTCTAA ATCTCGGGAG AGATGTCTAA
     2101 GATCAATTTA GGGATAACAG GGTAATGATC GGCTGACCGT GCCGATGCGC TGAGGTGTCA
     2161 ATCGTCGGAG CCGCTGAGCA ATAACTAGCA TAACCCCTTG GGGCCTCTAA ACGGGTCTTG
     2221 AGGGGTTTTT TGCATGGTCA TAGCTGTTTC CTGAGAGCTT GGCAGGTGAT GACACACATT
     2281 AACAAATTTC GTGAGGAGTC TCCAGAAGAA TGCCATTAAT TTCCATAGGC TCCGCCCCCC
     2341 TGACGAGCAT CACAAAAATC GACGCTCAAG TCAGAGGTGG CGAAACCCGA CAGGACTATA
     2401 AAGATACCAG GCGTTTCCCC CTGGAAGCTC CCTCGTGCGC TCTCCTGTTC CGACCCTGCC
     2461 GCTTACCGGA TACCTGTCCG CCTTTCTCCC TTCGGGAAGC GTGGCGCTTT CTCATAGCTC
     2521 ACGCTGTAGG TATCTCAGTT CGGTGTAGGT CGTTCGCTCC AAGCTGGGCT GTGTGCACGA
     2581 ACCCCCCGTT CAGCCCGACC GCTGCGCCTT ATCCGGTAAC TATCGTCTTG AGTCCAACCC
     2641 GGTAAGACAC GACTTATCGC CACTGGCAGC AGCCACTGGT AACAGGATTA GCAGAGCGAG
     2701 GTATGTAGGC GGTGCTACAG AGTTCTTGAA GTGGTGGCCT AACTACGGCT ACACTAGAAG
     2761 AACAGTATTT GGTATCTGCG CTCTGCTGAA GCCAGTTACC TTCGGAAAAA GAGTTGGTAG
     2821 CTCTTGATCC GGCAAACAAA CCACCGCTGG TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA
     2881 GATTACGCGC AGAAAAAAAG GATCTCAAGA AGGCCTACTA TTAGCAACAA CGATCCTTTG
     2941 ATCTTTTCTA CGGGGTCTGA CGCTCAGTGG AACGAAAACT CACGTTAAGG GATTTTGGTC
     3001 ATGAGATTAT CAAAAAGGAT CTTCACCTAG ATCCTTTTAA ATTAAAAATG AAGTTTTAAA
     3061 TCAATCTAAA GTATATATGA GTAAACTTGG TCTGACAGTT ACCAATGCTT AATCAGTGAG
     3121 GCACCTATCT CAGCGATCTG TCTATTTCGT TCATCCATAG TTGCCTGACT CCCCGTCGTG
     3181 TAGATAACTA CGATACGGGA GGGCTTACCA TCTGGCCCCA GTGCTGCAAT GATACCGCGA
     3241 GAACCACGCT CACCGGCTCC AGATTTATCA GCAATAAACC AGCCAGCCGG AAGGGCCGAG
     3301 CGCAGAAGTG GTCCTGCAAC TTTATCCGCC TCCATCCAGT CTATTAATTG TTGCCGGGAA
     3361 GCTAGAGTAA GTAGTTCGCC AGTTAATAGT TTGCGCAACG TTGTTGCCAT TGCTACAGGC
     3421 ATCGTGGTGT CACGCTCGTC GTTTGGTATG GCTTCATTCA GCTCCGGTTC CCAACGATCA
     3481 AGGCGAGTTA CATGATCCCC CATGTTGTGC AAAAAAGCGG TTAGCTCCTT CGGTCCTCCG
     3541 ATCGTTGTCA GAAGTAAGTT GGCCGCAGTG TTATCACTCA TGGTTATGGC AGCACTGCAT
     3601 AATTCTCTTA CTGTCATGCC ATCCGTAAGA TGCTTTTCTG TGACTGGTGA GTACTCAACC
     3661 AAGTCATTCT GAGAATAGTG TATGCGGCGA CCGAGTTGCT CTTGCCCGGC GTCAATACGG
     3721 GATAATACCG CGCCACATAG CAGAACTTTA AAAGTGCTCA TCATTGGAAA ACGTTCTTCG
     3781 GGGCGAAAAC TCTCAAGGAT CTTACCGCTG TTGAGATCCA GTTCGATGTA ACCCACTCGT
     3841 GCACCCAACT GATCTTCAGC ATCTTTTACT TTCACCAGCG TTTCTGGGTG AGCAAAAACA
     3901 GGAAGGCAAA ATGCCGCAAA AAAGGGAATA AGGGCGACAC GGAAATGTTG AATACTCATA
     3961 CTCTTCCTTT TTCAATATTA TTGAAGCATT TATCAGGGTT ATTGTCTCAT GAGCGGATAC
     4021 ATATTTGAAT GTATTTAGAA AAATAAACAA ATAGGGGTTC CGCGCACCTG CACCAGTCAG
     4081 TAAAACGACG GCCAGTAGTC AAAAGCCTCC GACCGGAGGC TTTTGACTTG GTTCAGGTGG
     4141 AGTGGGAG
//