LOCUS       Exported     3311 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..3311
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
                     /label="T7 terminator"
     terminator      1348..1395
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     3243..3259
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      3260..3291
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     primer_bind     1..20
                     /label="dialout_for"
                     /note="Reverse dialout primer. Unique pair for each FreeGenes part."
     primer_bind     1231..1250
                     /label="dialout_rev"
                     /note="Forward dialout primer. Unique pair for each FreeGenes part."
     CDS             29..1216
                     /translation="MSSLAPWRTTSWSPLGSPPSLEDALRLARTTRAFAVRRDGEGRALVTYLYGTPELFSLPGARELRGIVYREEDGTVLSRPFHKFFNFGEPLAPGEEAFKAFRDSMVPLFVAEKVDGYLAQAYLDGGELRFASRHSLNPPLVGALLRKAVDEEAMARLGKLLAAEGGRWTALLEVVDPEAPVMVPYQEPGVYLLALRSIGEGHYLLPGVHFPLPEALRYVRWEPRMDFDPHRFRGEIRDLQGVEGYVVTDGAEFVKFKTGWAFRLARFLMDPEGVFLEAYAEDRLDDLVGALAGREDLLRAVARAQDYLAGLYGEAVGAGDALRRMGLPRKEAWARVQEEAGRWGGFAPAYARAAMAAYEGGEAREAFLVELRKRSARKALEALHLFPRVGGELRG*"
                     /label="T4 RNA Ligase 1"
                     /note=""
     primer_bind     complement(1400..1416)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(1483..2071)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(2262..3122)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(3123..3227)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
     misc_feature    1251..1310
                     /label="FreeGenes Barcode"
                     /note="FreeGenes Barcode. Defined as I-SceI site flanked by 21 base pair random sequences."
ORIGIN
        1 AAAGGGGCCG TCAATATCAG GGTCTCTAAT GTCCTCACTG GCGCCATGGC GCACCACCTC
       61 GTGGTCCCCG CTTGGCAGCC CACCGAGCCT GGAAGATGCT CTGCGTTTAG CCCGTACCAC
      121 TCGCGCCTTC GCGGTTCGCC GCGACGGTGA AGGCCGCGCG TTAGTGACCT ACCTCTACGG
      181 GACCCCTGAA CTGTTCTCCC TGCCGGGCGC ACGTGAACTG CGCGGTATTG TATATCGTGA
      241 AGAGGACGGT ACTGTGCTGA GCCGTCCATT TCACAAGTTT TTCAACTTCG GTGAACCGCT
      301 GGCACCGGGT GAAGAAGCAT TCAAAGCATT CCGTGATTCT ATGGTTCCGC TGTTTGTCGC
      361 AGAAAAAGTT GATGGCTACC TGGCGCAGGC GTACCTGGAC GGCGGTGAAC TCCGTTTTGC
      421 GAGCCGTCAC TCTCTGAATC CTCCATTGGT AGGCGCTCTG CTGCGCAAAG CAGTGGACGA
      481 AGAGGCCATG GCCCGTCTGG GCAAACTGCT GGCGGCGGAA GGCGGCCGTT GGACGGCCCT
      541 GCTGGAGGTT GTTGACCCGG AAGCACCGGT TATGGTTCCG TACCAGGAGC CGGGTGTTTA
      601 CCTCCTTGCC CTGCGCTCTA TCGGCGAAGG TCACTACCTG CTGCCGGGTG TTCACTTCCC
      661 GCTGCCGGAA GCGCTGCGCT ACGTTCGTTG GGAACCGCGC ATGGACTTCG ATCCGCATCG
      721 TTTTCGTGGT GAAATCCGCG ATCTTCAGGG CGTGGAGGGC TACGTGGTGA CCGATGGCGC
      781 GGAGTTCGTC AAGTTCAAAA CCGGCTGGGC GTTTCGTCTG GCTCGTTTCC TGATGGACCC
      841 GGAAGGTGTT TTTCTCGAAG CGTACGCAGA GGACCGCCTG GATGACCTGG TAGGTGCACT
      901 GGCTGGCCGT GAGGACCTGC TGCGCGCCGT CGCCCGCGCG CAGGATTACC TGGCTGGCCT
      961 GTACGGCGAG GCTGTGGGCG CGGGCGACGC CCTGCGACGT ATGGGCCTGC CGCGTAAAGA
     1021 AGCGTGGGCC CGCGTACAGG AAGAAGCCGG TCGCTGGGGG GGCTTCGCGC CAGCTTACGC
     1081 TCGCGCTGCT ATGGCGGCAT ACGAAGGTGG TGAAGCTCGT GAGGCGTTCC TGGTCGAACT
     1141 GCGCAAGCGT AGCGCACGTA AAGCACTGGA AGCGCTGCAC CTGTTTCCAC GTGTGGGTGG
     1201 CGAACTGCGC GGCTGAAGAG CTTTGAGACC AGCTATAAGA ATTGCCGGGC AACATGGTGC
     1261 GGCGACATAA TTAGGGATAA CAGGGTAATC TGATAAAGAA TATGATTCAA CGCTGAGGTG
     1321 TCAATCGTCG GAGCCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC TAAACGGGTC
     1381 TTGAGGGGTT TTTTGCATGG TCATAGCTGT TTCCTGAGAG CTTGGCAGGT GATGACACAC
     1441 ATTAACAAAT TTCGTGAGGA GTCTCCAGAA GAATGCCATT AATTTCCATA GGCTCCGCCC
     1501 CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC CGACAGGACT
     1561 ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG TTCCGACCCT
     1621 GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC TTTCTCATAG
     1681 CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA
     1741 CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC TTGAGTCCAA
     1801 CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA TTAGCAGAGC
     1861 GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG GCTACACTAG
     1921 AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG
     1981 TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA
     2041 GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGGCCTA CTATTAGCAA CAACGATCCT
     2101 TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA AGGGATTTTG
     2161 GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA ATGAAGTTTT
     2221 AAATCAATCT AAAGTATATA TGAGTAAACT TGGTCTGACA GTTACCAATG CTTAATCAGT
     2281 GAGGCACCTA TCTCAGCGAT CTGTCTATTT CGTTCATCCA TAGTTGCCTG ACTCCCCGTC
     2341 GTGTAGATAA CTACGATACG GGAGGGCTTA CCATCTGGCC CCAGTGCTGC AATGATACCG
     2401 CGAGAACCAC GCTCACCGGC TCCAGATTTA TCAGCAATAA ACCAGCCAGC CGGAAGGGCC
     2461 GAGCGCAGAA GTGGTCCTGC AACTTTATCC GCCTCCATCC AGTCTATTAA TTGTTGCCGG
     2521 GAAGCTAGAG TAAGTAGTTC GCCAGTTAAT AGTTTGCGCA ACGTTGTTGC CATTGCTACA
     2581 GGCATCGTGG TGTCACGCTC GTCGTTTGGT ATGGCTTCAT TCAGCTCCGG TTCCCAACGA
     2641 TCAAGGCGAG TTACATGATC CCCCATGTTG TGCAAAAAAG CGGTTAGCTC CTTCGGTCCT
     2701 CCGATCGTTG TCAGAAGTAA GTTGGCCGCA GTGTTATCAC TCATGGTTAT GGCAGCACTG
     2761 CATAATTCTC TTACTGTCAT GCCATCCGTA AGATGCTTTT CTGTGACTGG TGAGTACTCA
     2821 ACCAAGTCAT TCTGAGAATA GTGTATGCGG CGACCGAGTT GCTCTTGCCC GGCGTCAATA
     2881 CGGGATAATA CCGCGCCACA TAGCAGAACT TTAAAAGTGC TCATCATTGG AAAACGTTCT
     2941 TCGGGGCGAA AACTCTCAAG GATCTTACCG CTGTTGAGAT CCAGTTCGAT GTAACCCACT
     3001 CGTGCACCCA ACTGATCTTC AGCATCTTTT ACTTTCACCA GCGTTTCTGG GTGAGCAAAA
     3061 ACAGGAAGGC AAAATGCCGC AAAAAAGGGA ATAAGGGCGA CACGGAAATG TTGAATACTC
     3121 ATACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT CATGAGCGGA
     3181 TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC CTGCACCAGT
     3241 CAGTAAAACG ACGGCCAGTA GTCAAAAGCC TCCGACCGGA GGCTTTTGAC TTGGTTCAGG
     3301 TGGAGTGGGA G
//