LOCUS       Exported     3725 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..3725
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      1762..1809
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     3657..3673
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      3674..3705
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     primer_bind     1..20
                     /label="dialout_for"
                     /note="Reverse dialout primer. Unique pair for each FreeGenes part."
     primer_bind     1645..1664
                     /label="dialout_rev"
                     /note="Forward dialout primer. Unique pair for each FreeGenes part."
     CDS             29..1630
                     /label="CIP (calf intestinal phosphatase)"
                     /note=""
                     /translation="MQGACVLLLLGLHLQLSLGLVPVEEEDPAFWNRQAAQALDVAKKLQPIQTAAKNVILFLGDGMGVPTVTATRILKGQMNGKLGPETPLAMDQFPYVALSKTYNVDRQVPDSAGTATAYLCGVKGNYRTIGVSAAARYNQCKTTRGNEVTSVMNRAKKAGKSVGVVTTTRVQHASPAGAYAHTVNRNWYSDADLPADAQMNGCQDIAAQLVNNMDIDVILGGGRKYMFPVGTPDPEYPDDASVNGVRKRKQNLVQAWQAKHQGAQYVWNRTALLQAADDSSVTHLMGLFEPADMKYNVQQDHTKDPTLQEMTEVALRVVSRNPRGFYLFVEGGRIDHGHHDDKAYMALTEAGMFDNAIAKANELTSELDTLILVTADHSHVFSFGGYTLRGTSIFGLAPSKALDSKSYTSILYGNGPGYALGGGSRPDVNDSTSEDPSYQQQAAVPQASETHGGEDVAVFARGPQAHLVHGVEEETFVAHIMAFAGCVEPYTDCNLPAPTTATSIPDAAHLAASPPPLALLAGAMLLLLAPTLY*"
     primer_bind     complement(1814..1830)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(1897..2485)
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
                     /label="ori"
     CDS             complement(2676..3536)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(3537..3641)
                     /note="ampicillin resistance gene promoter"
                     /label="bla promoter"
     misc_feature    1665..1724
                     /label="FreeGenes Barcode"
                     /note="FreeGenes Barcode. Defined as I-SceI site flanked by 21 base pair random sequences."
ORIGIN
        1 AAAGGGGCCG TCAATATCAG GGTCTCTAAT GCAGGGCGCA TGCGTTCTGC TGCTGCTGGG
       61 GCTGCACCTG CAACTGAGCC TGGGCCTGGT ACCGGTTGAA GAAGAGGACC CAGCGTTCTG
      121 GAACCGTCAG GCTGCGCAGG CGCTGGATGT TGCAAAGAAA CTCCAGCCGA TTCAAACTGC
      181 GGCAAAAAAC GTTATCCTGT TTCTGGGTGA CGGTATGGGT GTTCCTACGG TTACGGCAAC
      241 TCGTATTCTG AAAGGTCAGA TGAACGGCAA ACTGGGCCCT GAAACCCCGC TGGCGATGGA
      301 TCAGTTCCCG TACGTAGCCC TGAGCAAAAC CTATAACGTA GATCGTCAGG TTCCAGATTC
      361 TGCGGGCACC GCAACCGCAT ACCTGTGCGG CGTTAAAGGT AACTATCGCA CAATCGGCGT
      421 TTCTGCGGCA GCGCGTTACA ACCAGTGCAA AACTACTCGC GGCAACGAAG TCACCTCCGT
      481 GATGAACCGT GCCAAAAAAG CTGGTAAAAG CGTTGGTGTA GTAACTACCA CTCGCGTGCA
      541 GCACGCTTCT CCGGCGGGCG CCTACGCACA CACTGTGAAC CGTAATTGGT ACTCCGACGC
      601 GGATCTGCCG GCGGACGCTC AGATGAACGG TTGCCAGGAT ATTGCAGCCC AACTGGTAAA
      661 CAACATGGAT ATTGATGTTA TCCTGGGCGG CGGCCGGAAA TACATGTTTC CGGTTGGTAC
      721 CCCGGACCCG GAGTATCCGG ATGATGCGAG CGTCAACGGC GTACGCAAAA GAAAACAAAA
      781 CCTGGTGCAG GCGTGGCAGG CTAAGCACCA GGGTGCCCAA TACGTTTGGA ACCGTACTGC
      841 CCTGCTGCAA GCTGCTGACG ATTCTTCCGT AACGCACCTG ATGGGCCTGT TTGAGCCGGC
      901 TGATATGAAA TATAACGTGC AGCAAGATCA CACCAAAGAT CCGACGCTGC AAGAAATGAC
      961 TGAGGTAGCG CTGCGTGTGG TGTCGCGCAA CCCGCGCGGT TTCTATCTGT TTGTAGAAGG
     1021 CGGTCGTATC GATCATGGTC ACCACGACGA TAAAGCGTAT ATGGCGCTGA CCGAGGCTGG
     1081 TATGTTCGAC AACGCCATTG CCAAAGCGAA CGAACTGACG TCGGAACTTG ATACCCTGAT
     1141 CCTGGTTACC GCCGATCACA GCCACGTTTT CTCCTTCGGC GGTTATACCC TGCGTGGCAC
     1201 CAGCATTTTT GGCCTGGCTC CGAGCAAAGC ACTGGATAGC AAATCCTACA CCTCCATTCT
     1261 GTATGGCAAC GGTCCGGGCT ACGCGCTGGG CGGTGGCAGC CGTCCGGACG TCAATGACTC
     1321 CACCTCTGAG GACCCGTCCT ACCAGCAGCA GGCGGCTGTC CCGCAGGCGA GCGAAACCCA
     1381 CGGTGGCGAA GATGTTGCTG TGTTCGCTCG TGGTCCGCAG GCTCATCTGG TTCACGGCGT
     1441 TGAAGAGGAA ACCTTTGTCG CACATATCAT GGCTTTCGCT GGCTGCGTTG AGCCGTATAC
     1501 TGATTGTAAC CTGCCGGCTC CGACCACCGC AACCTCTATC CCGGACGCAG CACACCTGGC
     1561 GGCGAGCCCG CCTCCGCTGG CCCTGCTCGC CGGCGCTATG CTGCTGCTCC TGGCCCCGAC
     1621 CCTGTACTGA AGAGCTTTGA GACCAGCTAT GATCCCGGTG TAACGGCCGT GCGATAGATT
     1681 CCTACTAGGG ATAACAGGGT AATTGGCCGA ACCTGGAGCG GCTCCGCTGA GGTGTCAATC
     1741 GTCGGAGCCG CTGAGCAATA ACTAGCATAA CCCCTTGGGG CCTCTAAACG GGTCTTGAGG
     1801 GGTTTTTTGC ATGGTCATAG CTGTTTCCTG AGAGCTTGGC AGGTGATGAC ACACATTAAC
     1861 AAATTTCGTG AGGAGTCTCC AGAAGAATGC CATTAATTTC CATAGGCTCC GCCCCCCTGA
     1921 CGAGCATCAC AAAAATCGAC GCTCAAGTCA GAGGTGGCGA AACCCGACAG GACTATAAAG
     1981 ATACCAGGCG TTTCCCCCTG GAAGCTCCCT CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT
     2041 TACCGGATAC CTGTCCGCCT TTCTCCCTTC GGGAAGCGTG GCGCTTTCTC ATAGCTCACG
     2101 CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT TCGCTCCAAG CTGGGCTGTG TGCACGAACC
     2161 CCCCGTTCAG CCCGACCGCT GCGCCTTATC CGGTAACTAT CGTCTTGAGT CCAACCCGGT
     2221 AAGACACGAC TTATCGCCAC TGGCAGCAGC CACTGGTAAC AGGATTAGCA GAGCGAGGTA
     2281 TGTAGGCGGT GCTACAGAGT TCTTGAAGTG GTGGCCTAAC TACGGCTACA CTAGAAGAAC
     2341 AGTATTTGGT ATCTGCGCTC TGCTGAAGCC AGTTACCTTC GGAAAAAGAG TTGGTAGCTC
     2401 TTGATCCGGC AAACAAACCA CCGCTGGTAG CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT
     2461 TACGCGCAGA AAAAAAGGAT CTCAAGAAGG CCTACTATTA GCAACAACGA TCCTTTGATC
     2521 TTTTCTACGG GGTCTGACGC TCAGTGGAAC GAAAACTCAC GTTAAGGGAT TTTGGTCATG
     2581 AGATTATCAA AAAGGATCTT CACCTAGATC CTTTTAAATT AAAAATGAAG TTTTAAATCA
     2641 ATCTAAAGTA TATATGAGTA AACTTGGTCT GACAGTTACC AATGCTTAAT CAGTGAGGCA
     2701 CCTATCTCAG CGATCTGTCT ATTTCGTTCA TCCATAGTTG CCTGACTCCC CGTCGTGTAG
     2761 ATAACTACGA TACGGGAGGG CTTACCATCT GGCCCCAGTG CTGCAATGAT ACCGCGAGAA
     2821 CCACGCTCAC CGGCTCCAGA TTTATCAGCA ATAAACCAGC CAGCCGGAAG GGCCGAGCGC
     2881 AGAAGTGGTC CTGCAACTTT ATCCGCCTCC ATCCAGTCTA TTAATTGTTG CCGGGAAGCT
     2941 AGAGTAAGTA GTTCGCCAGT TAATAGTTTG CGCAACGTTG TTGCCATTGC TACAGGCATC
     3001 GTGGTGTCAC GCTCGTCGTT TGGTATGGCT TCATTCAGCT CCGGTTCCCA ACGATCAAGG
     3061 CGAGTTACAT GATCCCCCAT GTTGTGCAAA AAAGCGGTTA GCTCCTTCGG TCCTCCGATC
     3121 GTTGTCAGAA GTAAGTTGGC CGCAGTGTTA TCACTCATGG TTATGGCAGC ACTGCATAAT
     3181 TCTCTTACTG TCATGCCATC CGTAAGATGC TTTTCTGTGA CTGGTGAGTA CTCAACCAAG
     3241 TCATTCTGAG AATAGTGTAT GCGGCGACCG AGTTGCTCTT GCCCGGCGTC AATACGGGAT
     3301 AATACCGCGC CACATAGCAG AACTTTAAAA GTGCTCATCA TTGGAAAACG TTCTTCGGGG
     3361 CGAAAACTCT CAAGGATCTT ACCGCTGTTG AGATCCAGTT CGATGTAACC CACTCGTGCA
     3421 CCCAACTGAT CTTCAGCATC TTTTACTTTC ACCAGCGTTT CTGGGTGAGC AAAAACAGGA
     3481 AGGCAAAATG CCGCAAAAAA GGGAATAAGG GCGACACGGA AATGTTGAAT ACTCATACTC
     3541 TTCCTTTTTC AATATTATTG AAGCATTTAT CAGGGTTATT GTCTCATGAG CGGATACATA
     3601 TTTGAATGTA TTTAGAAAAA TAAACAAATA GGGGTTCCGC GCACCTGCAC CAGTCAGTAA
     3661 AACGACGGCC AGTAGTCAAA AGCCTCCGAC CGGAGGCTTT TGACTTGGTT CAGGTGGAGT
     3721 GGGAG
//