LOCUS Exported 2562 bp DNA circular SYN 24-JUL-2020 DEFINITION synthetic linear DNA ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2001) AUTHORS Keoni Gandall TITLE Direct Submission JOURNAL Exported from Poly. FEATURES Location/Qualifiers terminator 1..2562 /label="T7 terminator" /note="transcription terminator for bacteriophage T7 RNA polymerase" terminator 599..646 /note="transcription terminator for bacteriophage T7 RNA polymerase" /label="T7 terminator" primer_bind 2494..2510 /label="M13 for" /note="common sequencing primer, one of multiple similar variants" terminator 2511..2542 /label="tonB terminator" /note="bidirectional E. coli tonB-P14 transcription terminator" primer_bind 1..20 /label="dialout_for" /note="Reverse dialout primer. Unique pair for each FreeGenes part." primer_bind 482..501 /label="dialout_rev" /note="Forward dialout primer. Unique pair for each FreeGenes part." CDS 100..2561 /label="RNAse A" /note="" /translation="MALKSLVLLSLLVLVLLLVRVQPSLGKETAAAKFERQHMDSSTSAASSSNYCNQMMKSRNLTKDRCKPVNTFVHESLADVQAVCSQKNVACKNGQTNCYQSYSTMSITDCRETGSSKYPNCAYKTTQANKHIIVACEGNPYVPVHF" primer_bind complement(651..667) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" rep_origin complement(734..1322) /label="ori" /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(1513..2373) /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*" /label="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" promoter complement(2374..2478) /label="bla promoter" /note="ampicillin resistance gene promoter" misc_feature 502..561 /label="FreeGenes Barcode" /note="FreeGenes Barcode. Defined as I-SceI site flanked by 21 base pair random sequences." ORIGIN 1 AAAGGGGCCG TCAATATCAG GGTCTCTAAT GGCACTGAAA TCTCTCGTGC TGCTCTCTCT 61 GCTGGTACTG GTTCTGCTGC TGGTTCGCGT GCAGCCGTCG CTGGGCAAGG AAACCGCTGC 121 GGCCAAATTC GAACGTCAGC ACATGGACTC GTCTACCTCT GCCGCATCCT CTAGCAACTA 181 CTGCAACCAA ATGATGAAAT CTCGCAACCT GACCAAGGAT CGTTGCAAAC CGGTTAACAC 241 CTTCGTGCAT GAATCCCTGG CAGACGTCCA GGCTGTTTGC TCCCAGAAGA ACGTAGCATG 301 CAAGAACGGC CAGACCAACT GTTATCAGTC TTATTCGACG ATGAGCATCA CCGATTGCCG 361 TGAAACTGGT TCCTCAAAAT ATCCTAACTG CGCGTATAAA ACAACTCAGG CCAACAAACA 421 TATTATCGTG GCTTGCGAAG GCAACCCTTA CGTGCCGGTG CACTTTGATG CTTCTGTTTG 481 AAGCTCAATC TAACAGTGGG GCACGAGGCT CGCCGTTGCT ATTAGGGATA ACAGGGTAAT 541 GGCCATAGAG AGTGAAATTA ACGCTGAGGT GTCAATCGTC GGAGCCGCTG AGCAATAACT 601 AGCATAACCC CTTGGGGCCT CTAAACGGGT CTTGAGGGGT TTTTTGCATG GTCATAGCTG 661 TTTCCTGAGA GCTTGGCAGG TGATGACACA CATTAACAAA TTTCGTGAGG AGTCTCCAGA 721 AGAATGCCAT TAATTTCCAT AGGCTCCGCC CCCCTGACGA GCATCACAAA AATCGACGCT 781 CAAGTCAGAG GTGGCGAAAC CCGACAGGAC TATAAAGATA CCAGGCGTTT CCCCCTGGAA 841 GCTCCCTCGT GCGCTCTCCT GTTCCGACCC TGCCGCTTAC CGGATACCTG TCCGCCTTTC 901 TCCCTTCGGG AAGCGTGGCG CTTTCTCATA GCTCACGCTG TAGGTATCTC AGTTCGGTGT 961 AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC ACGAACCCCC CGTTCAGCCC GACCGCTGCG 1021 CCTTATCCGG TAACTATCGT CTTGAGTCCA ACCCGGTAAG ACACGACTTA TCGCCACTGG 1081 CAGCAGCCAC TGGTAACAGG ATTAGCAGAG CGAGGTATGT AGGCGGTGCT ACAGAGTTCT 1141 TGAAGTGGTG GCCTAACTAC GGCTACACTA GAAGAACAGT ATTTGGTATC TGCGCTCTGC 1201 TGAAGCCAGT TACCTTCGGA AAAAGAGTTG GTAGCTCTTG ATCCGGCAAA CAAACCACCG 1261 CTGGTAGCGG TGGTTTTTTT GTTTGCAAGC AGCAGATTAC GCGCAGAAAA AAAGGATCTC 1321 AAGAAGGCCT ACTATTAGCA ACAACGATCC TTTGATCTTT TCTACGGGGT CTGACGCTCA 1381 GTGGAACGAA AACTCACGTT AAGGGATTTT GGTCATGAGA TTATCAAAAA GGATCTTCAC 1441 CTAGATCCTT TTAAATTAAA AATGAAGTTT TAAATCAATC TAAAGTATAT ATGAGTAAAC 1501 TTGGTCTGAC AGTTACCAAT GCTTAATCAG TGAGGCACCT ATCTCAGCGA TCTGTCTATT 1561 TCGTTCATCC ATAGTTGCCT GACTCCCCGT CGTGTAGATA ACTACGATAC GGGAGGGCTT 1621 ACCATCTGGC CCCAGTGCTG CAATGATACC GCGAGAACCA CGCTCACCGG CTCCAGATTT 1681 ATCAGCAATA AACCAGCCAG CCGGAAGGGC CGAGCGCAGA AGTGGTCCTG CAACTTTATC 1741 CGCCTCCATC CAGTCTATTA ATTGTTGCCG GGAAGCTAGA GTAAGTAGTT CGCCAGTTAA 1801 TAGTTTGCGC AACGTTGTTG CCATTGCTAC AGGCATCGTG GTGTCACGCT CGTCGTTTGG 1861 TATGGCTTCA TTCAGCTCCG GTTCCCAACG ATCAAGGCGA GTTACATGAT CCCCCATGTT 1921 GTGCAAAAAA GCGGTTAGCT CCTTCGGTCC TCCGATCGTT GTCAGAAGTA AGTTGGCCGC 1981 AGTGTTATCA CTCATGGTTA TGGCAGCACT GCATAATTCT CTTACTGTCA TGCCATCCGT 2041 AAGATGCTTT TCTGTGACTG GTGAGTACTC AACCAAGTCA TTCTGAGAAT AGTGTATGCG 2101 GCGACCGAGT TGCTCTTGCC CGGCGTCAAT ACGGGATAAT ACCGCGCCAC ATAGCAGAAC 2161 TTTAAAAGTG CTCATCATTG GAAAACGTTC TTCGGGGCGA AAACTCTCAA GGATCTTACC 2221 GCTGTTGAGA TCCAGTTCGA TGTAACCCAC TCGTGCACCC AACTGATCTT CAGCATCTTT 2281 TACTTTCACC AGCGTTTCTG GGTGAGCAAA AACAGGAAGG CAAAATGCCG CAAAAAAGGG 2341 AATAAGGGCG ACACGGAAAT GTTGAATACT CATACTCTTC CTTTTTCAAT ATTATTGAAG 2401 CATTTATCAG GGTTATTGTC TCATGAGCGG ATACATATTT GAATGTATTT AGAAAAATAA 2461 ACAAATAGGG GTTCCGCGCA CCTGCACCAG TCAGTAAAAC GACGGCCAGT AGTCAAAAGC 2521 CTCCGACCGG AGGCTTTTGA CTTGGTTCAG GTGGAGTGGG AG //