LOCUS Exported 2957 bp DNA circular SYN 24-JUL-2020 DEFINITION synthetic linear DNA ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2001) AUTHORS Keoni Gandall TITLE Direct Submission JOURNAL Exported from Poly. FEATURES Location/Qualifiers terminator 1..2957 /label="T7 terminator" /note="transcription terminator for bacteriophage T7 RNA polymerase" terminator 994..1041 /label="T7 terminator" /note="transcription terminator for bacteriophage T7 RNA polymerase" primer_bind 2889..2905 /label="M13 for" /note="common sequencing primer, one of multiple similar variants" terminator 2906..2937 /label="tonB terminator" /note="bidirectional E. coli tonB-P14 transcription terminator" primer_bind 1..20 /label="dialout_for" /note="Reverse dialout primer. Unique pair for each FreeGenes part." primer_bind 877..896 /label="dialout_rev" /note="Forward dialout primer. Unique pair for each FreeGenes part." CDS 29..862 /label="EcoRI" /note="" /translation="MSNKKQSNRLTEQHKLSQGVIGIFGDYAKAHDLAVGEVSKLVKKALSNEYPQLSFRYRDSIKKTEINEALKKIDPDLGGTLFVSNSSIKPDGGIVEVKDDYGEWRVVLVAEAKHQGKDIINIRNGLLVGKRGDQDLMAAGNAIERSHKNISEIANFMLSESHFPYVLFLEGSNFLTENISITRPDGRVVNLEYNSGILNRLDRLTAANYGMPINSNLCINKFVNHKDKSIMLQAASIYTQGDGREWDSKIMFEIMFDISTTSLRVLGRDLFEQLTSK*" primer_bind complement(1046..1062) /label="M13 rev" /note="common sequencing primer, one of multiple similar variants" rep_origin complement(1129..1717) /label="ori" /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(1908..2768) /label="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*" promoter complement(2769..2873) /label="bla promoter" /note="ampicillin resistance gene promoter" misc_feature 897..956 /label="FreeGenes Barcode" /note="FreeGenes Barcode. Defined as I-SceI site flanked by 21 base pair random sequences." ORIGIN 1 AAAGGGGCCG TCAATATCAG GGTCTCTAAT GTCTAACAAA AAACAGTCCA ACCGTCTGAC 61 CGAACAGCAC AAACTGTCCC AAGGTGTTAT TGGTATCTTC GGCGACTACG CGAAAGCTCA 121 CGATCTGGCT GTAGGTGAAG TGTCCAAACT GGTTAAAAAA GCACTGTCTA ATGAATACCC 181 GCAGCTGTCT TTCCGTTACC GTGATTCTAT CAAAAAAACT GAAATTAACG AAGCTCTGAA 241 GAAAATCGAT CCGGACCTGG GTGGCACTCT GTTCGTGTCC AACTCCAGCA TCAAACCAGA 301 CGGCGGTATT GTTGAGGTTA AAGATGATTA CGGTGAATGG CGTGTAGTTC TGGTAGCGGA 361 GGCCAAGCAC CAGGGCAAAG ACATCATTAA CATTCGTAAT GGTCTGCTGG TGGGTAAACG 421 TGGTGACCAG GATTTAATGG CAGCGGGGAA CGCAATCGAA CGTTCTCATA AAAACATCAG 481 CGAAATCGCC AACTTTATGC TGAGCGAATC GCACTTCCCG TACGTTCTGT TCCTGGAAGG 541 CTCTAACTTT CTGACTGAAA ACATCTCTAT TACTCGTCCG GATGGCCGCG TTGTTAACCT 601 GGAATACAAC TCTGGTATTC TGAATCGCCT GGACCGTCTG ACTGCGGCCA ACTACGGCAT 661 GCCGATTAAC TCCAACCTGT GCATCAACAA ATTCGTCAAC CACAAAGACA AATCTATCAT 721 GCTGCAAGCA GCCAGCATCT ACACCCAGGG CGATGGCCGC GAGTGGGACT CGAAAATCAT 781 GTTCGAAATC ATGTTCGACA TCTCTACCAC CTCCCTGCGT GTTCTGGGCC GTGATCTGTT 841 CGAACAGCTG ACCTCTAAGT GAAGAGCTTT GAGACCAGGG CTAATTACCA TCAGCGCCAT 901 CAACACATAA TTGTTACTAG GGATAACAGG GTAATGGAGG TGTCTATACC ACCACTCGCT 961 GAGGTGTCAA TCGTCGGAGC CGCTGAGCAA TAACTAGCAT AACCCCTTGG GGCCTCTAAA 1021 CGGGTCTTGA GGGGTTTTTT GCATGGTCAT AGCTGTTTCC TGAGAGCTTG GCAGGTGATG 1081 ACACACATTA ACAAATTTCG TGAGGAGTCT CCAGAAGAAT GCCATTAATT TCCATAGGCT 1141 CCGCCCCCCT GACGAGCATC ACAAAAATCG ACGCTCAAGT CAGAGGTGGC GAAACCCGAC 1201 AGGACTATAA AGATACCAGG CGTTTCCCCC TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC 1261 GACCCTGCCG CTTACCGGAT ACCTGTCCGC CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC 1321 TCATAGCTCA CGCTGTAGGT ATCTCAGTTC GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG 1381 TGTGCACGAA CCCCCCGTTC AGCCCGACCG CTGCGCCTTA TCCGGTAACT ATCGTCTTGA 1441 GTCCAACCCG GTAAGACACG ACTTATCGCC ACTGGCAGCA GCCACTGGTA ACAGGATTAG 1501 CAGAGCGAGG TATGTAGGCG GTGCTACAGA GTTCTTGAAG TGGTGGCCTA ACTACGGCTA 1561 CACTAGAAGA ACAGTATTTG GTATCTGCGC TCTGCTGAAG CCAGTTACCT TCGGAAAAAG 1621 AGTTGGTAGC TCTTGATCCG GCAAACAAAC CACCGCTGGT AGCGGTGGTT TTTTTGTTTG 1681 CAAGCAGCAG ATTACGCGCA GAAAAAAAGG ATCTCAAGAA GGCCTACTAT TAGCAACAAC 1741 GATCCTTTGA TCTTTTCTAC GGGGTCTGAC GCTCAGTGGA ACGAAAACTC ACGTTAAGGG 1801 ATTTTGGTCA TGAGATTATC AAAAAGGATC TTCACCTAGA TCCTTTTAAA TTAAAAATGA 1861 AGTTTTAAAT CAATCTAAAG TATATATGAG TAAACTTGGT CTGACAGTTA CCAATGCTTA 1921 ATCAGTGAGG CACCTATCTC AGCGATCTGT CTATTTCGTT CATCCATAGT TGCCTGACTC 1981 CCCGTCGTGT AGATAACTAC GATACGGGAG GGCTTACCAT CTGGCCCCAG TGCTGCAATG 2041 ATACCGCGAG AACCACGCTC ACCGGCTCCA GATTTATCAG CAATAAACCA GCCAGCCGGA 2101 AGGGCCGAGC GCAGAAGTGG TCCTGCAACT TTATCCGCCT CCATCCAGTC TATTAATTGT 2161 TGCCGGGAAG CTAGAGTAAG TAGTTCGCCA GTTAATAGTT TGCGCAACGT TGTTGCCATT 2221 GCTACAGGCA TCGTGGTGTC ACGCTCGTCG TTTGGTATGG CTTCATTCAG CTCCGGTTCC 2281 CAACGATCAA GGCGAGTTAC ATGATCCCCC ATGTTGTGCA AAAAAGCGGT TAGCTCCTTC 2341 GGTCCTCCGA TCGTTGTCAG AAGTAAGTTG GCCGCAGTGT TATCACTCAT GGTTATGGCA 2401 GCACTGCATA ATTCTCTTAC TGTCATGCCA TCCGTAAGAT GCTTTTCTGT GACTGGTGAG 2461 TACTCAACCA AGTCATTCTG AGAATAGTGT ATGCGGCGAC CGAGTTGCTC TTGCCCGGCG 2521 TCAATACGGG ATAATACCGC GCCACATAGC AGAACTTTAA AAGTGCTCAT CATTGGAAAA 2581 CGTTCTTCGG GGCGAAAACT CTCAAGGATC TTACCGCTGT TGAGATCCAG TTCGATGTAA 2641 CCCACTCGTG CACCCAACTG ATCTTCAGCA TCTTTTACTT TCACCAGCGT TTCTGGGTGA 2701 GCAAAAACAG GAAGGCAAAA TGCCGCAAAA AAGGGAATAA GGGCGACACG GAAATGTTGA 2761 ATACTCATAC TCTTCCTTTT TCAATATTAT TGAAGCATTT ATCAGGGTTA TTGTCTCATG 2821 AGCGGATACA TATTTGAATG TATTTAGAAA AATAAACAAA TAGGGGTTCC GCGCACCTGC 2881 ACCAGTCAGT AAAACGACGG CCAGTAGTCA AAAGCCTCCG ACCGGAGGCT TTTGACTTGG 2941 TTCAGGTGGA GTGGGAG //