LOCUS       Exported     3104 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..3104
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      1141..1188
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     3036..3052
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      3053..3084
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     primer_bind     1..20
                     /note="Reverse dialout primer. Unique pair for each FreeGenes part."
                     /label="dialout_for"
     primer_bind     1024..1043
                     /label="dialout_rev"
                     /note="Forward dialout primer. Unique pair for each FreeGenes part."
     CDS             29..1009
                     /label="PstI"
                     /note=""
                     /translation="MKELKLKEAKEILKALGLPPQQYNDRSGWVLLALANIKPEDSWKEAKAPLLPTVSIMEFIRTEYGKDYKPNSRETIRRQTLHQFEQARIVDRNRDLPSRATNSKDNNYSLNQVIIDILHNYPNGNWKELIQQFLTHVPSLQELYERALARDRIPIKLLDGTQISLSPGEHNQLHADIVHEFCPRFVGDMGKILYIGDTASSRNEGGKLMVLDSEYLKKLGVPPMSHDKLPDVVVYDEKRKWLFLIEAVTSHGPISPKRWLELEAALSSCTVGKVYVTAFPTRTEFRKNAANIAWETEVWIADNPDHMVHFNGDRFLGPHDKKPELS*"
     primer_bind     complement(1193..1209)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(1276..1864)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(2055..2915)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(2916..3020)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
     misc_feature    1044..1103
                     /label="FreeGenes Barcode"
                     /note="FreeGenes Barcode. Defined as I-SceI site flanked by 21 base pair random sequences."
ORIGIN
        1 AAAGGGGCCG TCAATATCAG GGTCTCTAAT GAAGGAACTG AAACTGAAAG AAGCGAAGGA
       61 AATCCTGAAA GCGCTTGGCC TGCCGCCGCA GCAGTACAAC GACCGTTCTG GCTGGGTGCT
      121 ACTCGCGCTG GCGAACATTA AACCGGAAGA TTCTTGGAAA GAAGCGAAAG CGCCGCTGCT
      181 GCCGACCGTA TCCATTATGG AGTTCATTCG TACTGAATAC GGTAAAGACT ACAAACCGAA
      241 CTCCCGCGAA ACCATTCGTC GTCAGACCCT GCACCAGTTC GAACAGGCGC GCATCGTAGA
      301 CCGTAACCGT GACCTCCCGT CCCGTGCGAC TAACTCCAAA GATAACAACT ACTCCCTGAA
      361 CCAGGTAATC ATTGATATCC TGCATAACTA CCCTAACGGT AACTGGAAAG AGCTGATTCA
      421 GCAATTTCTG ACTCATGTCC CGAGCCTTCA GGAACTGTAT GAACGCGCCC TGGCGCGTGA
      481 CCGTATCCCG ATCAAACTGC TGGACGGCAC CCAGATCAGC CTGTCTCCAG GTGAACACAA
      541 CCAATTGCAT GCTGACATCG TACACGAATT TTGCCCGCGT TTCGTGGGTG ACATGGGCAA
      601 AATTCTGTAC ATCGGTGATA CTGCGTCCTC TCGTAACGAA GGTGGCAAAC TTATGGTTCT
      661 GGATAGCGAA TACCTGAAAA AGCTGGGCGT ACCTCCGATG TCCCACGATA AGCTGCCAGA
      721 TGTGGTTGTC TACGACGAAA AACGCAAATG GCTGTTCCTG ATCGAAGCAG TTACAAGCCA
      781 CGGCCCGATC TCACCGAAAC GTTGGCTGGA ACTGGAAGCA GCGCTGTCTA GCTGTACCGT
      841 TGGTAAAGTC TATGTTACCG CATTTCCGAC CCGTACGGAG TTCCGTAAGA ACGCAGCTAA
      901 CATCGCATGG GAAACCGAAG TTTGGATCGC TGATAACCCA GACCACATGG TTCACTTCAA
      961 CGGCGACCGT TTTCTCGGTC CACACGACAA AAAACCGGAG CTGTCCTGAA GAGCTTTGAG
     1021 ACCAGTAAAG CATAGTGCCC AGCCCCTATA GGTGTAGGGA AAGGTAGGGA TAACAGGGTA
     1081 ATGTGAACCA TGTCCCATCC TATCGCTGAG GTGTCAATCG TCGGAGCCGC TGAGCAATAA
     1141 CTAGCATAAC CCCTTGGGGC CTCTAAACGG GTCTTGAGGG GTTTTTTGCA TGGTCATAGC
     1201 TGTTTCCTGA GAGCTTGGCA GGTGATGACA CACATTAACA AATTTCGTGA GGAGTCTCCA
     1261 GAAGAATGCC ATTAATTTCC ATAGGCTCCG CCCCCCTGAC GAGCATCACA AAAATCGACG
     1321 CTCAAGTCAG AGGTGGCGAA ACCCGACAGG ACTATAAAGA TACCAGGCGT TTCCCCCTGG
     1381 AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC CCTGCCGCTT ACCGGATACC TGTCCGCCTT
     1441 TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA TAGCTCACGC TGTAGGTATC TCAGTTCGGT
     1501 GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT GCACGAACCC CCCGTTCAGC CCGACCGCTG
     1561 CGCCTTATCC GGTAACTATC GTCTTGAGTC CAACCCGGTA AGACACGACT TATCGCCACT
     1621 GGCAGCAGCC ACTGGTAACA GGATTAGCAG AGCGAGGTAT GTAGGCGGTG CTACAGAGTT
     1681 CTTGAAGTGG TGGCCTAACT ACGGCTACAC TAGAAGAACA GTATTTGGTA TCTGCGCTCT
     1741 GCTGAAGCCA GTTACCTTCG GAAAAAGAGT TGGTAGCTCT TGATCCGGCA AACAAACCAC
     1801 CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA GCAGCAGATT ACGCGCAGAA AAAAAGGATC
     1861 TCAAGAAGGC CTACTATTAG CAACAACGAT CCTTTGATCT TTTCTACGGG GTCTGACGCT
     1921 CAGTGGAACG AAAACTCACG TTAAGGGATT TTGGTCATGA GATTATCAAA AAGGATCTTC
     1981 ACCTAGATCC TTTTAAATTA AAAATGAAGT TTTAAATCAA TCTAAAGTAT ATATGAGTAA
     2041 ACTTGGTCTG ACAGTTACCA ATGCTTAATC AGTGAGGCAC CTATCTCAGC GATCTGTCTA
     2101 TTTCGTTCAT CCATAGTTGC CTGACTCCCC GTCGTGTAGA TAACTACGAT ACGGGAGGGC
     2161 TTACCATCTG GCCCCAGTGC TGCAATGATA CCGCGAGAAC CACGCTCACC GGCTCCAGAT
     2221 TTATCAGCAA TAAACCAGCC AGCCGGAAGG GCCGAGCGCA GAAGTGGTCC TGCAACTTTA
     2281 TCCGCCTCCA TCCAGTCTAT TAATTGTTGC CGGGAAGCTA GAGTAAGTAG TTCGCCAGTT
     2341 AATAGTTTGC GCAACGTTGT TGCCATTGCT ACAGGCATCG TGGTGTCACG CTCGTCGTTT
     2401 GGTATGGCTT CATTCAGCTC CGGTTCCCAA CGATCAAGGC GAGTTACATG ATCCCCCATG
     2461 TTGTGCAAAA AAGCGGTTAG CTCCTTCGGT CCTCCGATCG TTGTCAGAAG TAAGTTGGCC
     2521 GCAGTGTTAT CACTCATGGT TATGGCAGCA CTGCATAATT CTCTTACTGT CATGCCATCC
     2581 GTAAGATGCT TTTCTGTGAC TGGTGAGTAC TCAACCAAGT CATTCTGAGA ATAGTGTATG
     2641 CGGCGACCGA GTTGCTCTTG CCCGGCGTCA ATACGGGATA ATACCGCGCC ACATAGCAGA
     2701 ACTTTAAAAG TGCTCATCAT TGGAAAACGT TCTTCGGGGC GAAAACTCTC AAGGATCTTA
     2761 CCGCTGTTGA GATCCAGTTC GATGTAACCC ACTCGTGCAC CCAACTGATC TTCAGCATCT
     2821 TTTACTTTCA CCAGCGTTTC TGGGTGAGCA AAAACAGGAA GGCAAAATGC CGCAAAAAAG
     2881 GGAATAAGGG CGACACGGAA ATGTTGAATA CTCATACTCT TCCTTTTTCA ATATTATTGA
     2941 AGCATTTATC AGGGTTATTG TCTCATGAGC GGATACATAT TTGAATGTAT TTAGAAAAAT
     3001 AAACAAATAG GGGTTCCGCG CACCTGCACC AGTCAGTAAA ACGACGGCCA GTAGTCAAAA
     3061 GCCTCCGACC GGAGGCTTTT GACTTGGTTC AGGTGGAGTG GGAG
//