LOCUS       Exported     3647 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..3647
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      1684..1731
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     3579..3595
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      3596..3627
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     primer_bind     1..20
                     /label="dialout_for"
                     /note="Reverse dialout primer. Unique pair for each FreeGenes part."
     primer_bind     1567..1586
                     /label="dialout_rev"
                     /note="Forward dialout primer. Unique pair for each FreeGenes part."
     CDS             29..1552
                     /label="M.PstI"
                     /note=""
                     /translation="MTEAATQLPISLNILVDSIREAANSTLDETLRSKLGQFMSSSAVSELMANLFESYVGEHEILDAGAGVGSLTAAFVQNATLNGAKSISSTCYEISEVMVYNLIQVLDLCKIRAMEFEVNWQQKIIESDFIQASVEQLLIENYSPKYNKAILNPPYLKIAAKGRERALLQKVGIEASNLYSAFVALAIKQLKSGGELVAITPRSFCNGPYFNDFRKQMLDECSLNKIHVFNSRKSAFKADNVLQENIIYHLTKGETQRKVVTVYSSTCANDINPTIFEVPFDEIVKSNNPDLFIHIVTNEQERELANKAGGLPCSLSDLGIQVSTGKVVDFRTRENLSMEYISNSVPLIFPQHLQRCSIVWPITKAKKPNALIVNEATNNLMVPNGIYVLTRRLTAKEEKRRIVASIYYPDIANVDTVGFDNKINYFHANGKPLDISLAKGLWVFLNSTLIDKYFRQMNGHTQVNATDLRALRYPTREQLEDIANQVDFGEFEQTKIDEIINQSLQLM*"
     primer_bind     complement(1736..1752)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(1819..2407)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(2598..3458)
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
                     /label="bla"
     promoter        complement(3459..3563)
                     /note="ampicillin resistance gene promoter"
                     /label="bla promoter"
     misc_feature    1587..1646
                     /label="FreeGenes Barcode"
                     /note="FreeGenes Barcode. Defined as I-SceI site flanked by 21 base pair random sequences."
ORIGIN
        1 AAAGGGGCCG TCAATATCAG GGTCTCTAAT GACCGAAGCA GCGACTCAGC TCCCGATCAG
       61 CCTCAATATC CTCGTCGATA GCATTCGTGA GGCGGCTAAC AGCACTTTGG ACGAAACCCT
      121 CCGCTCTAAA CTGGGTCAGT TCATGTCCAG CAGCGCAGTG TCCGAACTGA TGGCAAACCT
      181 GTTCGAAAGC TATGTTGGTG AACACGAAAT CTTAGACGCT GGTGCGGGTG TGGGTTCCCT
      241 GACCGCCGCT TTCGTGCAGA ACGCAACCCT CAACGGGGCC AAATCTATCT CCTCGACTTG
      301 CTACGAAATC AGCGAAGTCA TGGTATACAA CCTTATTCAG GTTCTGGATC TGTGTAAGAT
      361 CCGTGCTATG GAGTTCGAAG TTAACTGGCA GCAGAAAATC ATCGAGTCCG ACTTCATTCA
      421 GGCTTCTGTT GAGCAGCTGC TGATCGAGAA CTACTCCCCG AAATATAACA AAGCAATCCT
      481 CAATCCGCCA TACTTGAAAA TCGCGGCGAA AGGCCGTGAA CGTGCTCTCC TGCAAAAAGT
      541 TGGTATTGAA GCGTCCAACC TGTATTCCGC ATTCGTCGCC CTGGCCATCA AACAGCTGAA
      601 ATCTGGCGGC GAACTGGTAG CTATCACGCC GCGCTCCTTC TGTAACGGTC CATATTTCAA
      661 CGACTTCCGC AAACAGATGC TGGATGAATG CTCCCTGAAC AAAATCCACG TGTTCAACAG
      721 CCGTAAATCT GCTTTCAAAG CAGACAACGT TCTGCAAGAA AATATCATCT ACCACCTGAC
      781 CAAAGGTGAA ACGCAGCGTA AAGTTGTAAC CGTTTACTCG TCCACTTGTG CTAACGATAT
      841 CAACCCGACC ATTTTCGAGG TGCCGTTCGA CGAAATTGTT AAGTCTAACA ACCCGGACCT
      901 GTTTATTCAC ATCGTAACCA ACGAACAGGA ACGTGAACTG GCGAACAAAG CAGGCGGCCT
      961 GCCATGTTCC CTGTCTGATC TGGGCATTCA GGTGTCCACG GGCAAAGTCG TTGATTTTCG
     1021 CACCCGTGAA AACCTGTCCA TGGAGTATAT TTCTAACTCC GTACCGCTGA TCTTCCCGCA
     1081 GCACCTGCAA CGTTGCAGCA TCGTATGGCC GATCACGAAA GCTAAAAAAC CGAACGCCCT
     1141 GATCGTAAAC GAAGCTACTA ACAATCTGAT GGTTCCGAAC GGGATTTACG TGCTGACTCG
     1201 TCGTCTGACT GCAAAAGAAG AAAAACGTCG TATCGTTGCG AGCATCTATT ACCCAGATAT
     1261 CGCCAACGTT GATACCGTTG GCTTCGATAA CAAAATCAAC TACTTTCACG CTAACGGCAA
     1321 ACCGCTTGAT ATCTCTCTGG CTAAGGGCCT GTGGGTTTTC CTGAACTCCA CTCTGATCGA
     1381 TAAATACTTC CGCCAAATGA ACGGTCACAC TCAGGTAAAC GCGACCGATC TGCGTGCCCT
     1441 CCGCTACCCA ACCCGTGAAC AGCTGGAGGA CATCGCTAAT CAGGTGGATT TTGGTGAGTT
     1501 CGAACAGACC AAAATCGACG AAATTATCAA CCAATCTCTG CAACTGATGT GAAGAGCTTT
     1561 GAGACCAGTA GTATCCGAAT CGCTGCTTGC ATACGGTTGA GAAAGCATAG GGATAACAGG
     1621 GTAATACTCT TTCAATTAAA CTCCCTCGCT GAGGTGTCAA TCGTCGGAGC CGCTGAGCAA
     1681 TAACTAGCAT AACCCCTTGG GGCCTCTAAA CGGGTCTTGA GGGGTTTTTT GCATGGTCAT
     1741 AGCTGTTTCC TGAGAGCTTG GCAGGTGATG ACACACATTA ACAAATTTCG TGAGGAGTCT
     1801 CCAGAAGAAT GCCATTAATT TCCATAGGCT CCGCCCCCCT GACGAGCATC ACAAAAATCG
     1861 ACGCTCAAGT CAGAGGTGGC GAAACCCGAC AGGACTATAA AGATACCAGG CGTTTCCCCC
     1921 TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC GACCCTGCCG CTTACCGGAT ACCTGTCCGC
     1981 CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC TCATAGCTCA CGCTGTAGGT ATCTCAGTTC
     2041 GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG TGTGCACGAA CCCCCCGTTC AGCCCGACCG
     2101 CTGCGCCTTA TCCGGTAACT ATCGTCTTGA GTCCAACCCG GTAAGACACG ACTTATCGCC
     2161 ACTGGCAGCA GCCACTGGTA ACAGGATTAG CAGAGCGAGG TATGTAGGCG GTGCTACAGA
     2221 GTTCTTGAAG TGGTGGCCTA ACTACGGCTA CACTAGAAGA ACAGTATTTG GTATCTGCGC
     2281 TCTGCTGAAG CCAGTTACCT TCGGAAAAAG AGTTGGTAGC TCTTGATCCG GCAAACAAAC
     2341 CACCGCTGGT AGCGGTGGTT TTTTTGTTTG CAAGCAGCAG ATTACGCGCA GAAAAAAAGG
     2401 ATCTCAAGAA GGCCTACTAT TAGCAACAAC GATCCTTTGA TCTTTTCTAC GGGGTCTGAC
     2461 GCTCAGTGGA ACGAAAACTC ACGTTAAGGG ATTTTGGTCA TGAGATTATC AAAAAGGATC
     2521 TTCACCTAGA TCCTTTTAAA TTAAAAATGA AGTTTTAAAT CAATCTAAAG TATATATGAG
     2581 TAAACTTGGT CTGACAGTTA CCAATGCTTA ATCAGTGAGG CACCTATCTC AGCGATCTGT
     2641 CTATTTCGTT CATCCATAGT TGCCTGACTC CCCGTCGTGT AGATAACTAC GATACGGGAG
     2701 GGCTTACCAT CTGGCCCCAG TGCTGCAATG ATACCGCGAG AACCACGCTC ACCGGCTCCA
     2761 GATTTATCAG CAATAAACCA GCCAGCCGGA AGGGCCGAGC GCAGAAGTGG TCCTGCAACT
     2821 TTATCCGCCT CCATCCAGTC TATTAATTGT TGCCGGGAAG CTAGAGTAAG TAGTTCGCCA
     2881 GTTAATAGTT TGCGCAACGT TGTTGCCATT GCTACAGGCA TCGTGGTGTC ACGCTCGTCG
     2941 TTTGGTATGG CTTCATTCAG CTCCGGTTCC CAACGATCAA GGCGAGTTAC ATGATCCCCC
     3001 ATGTTGTGCA AAAAAGCGGT TAGCTCCTTC GGTCCTCCGA TCGTTGTCAG AAGTAAGTTG
     3061 GCCGCAGTGT TATCACTCAT GGTTATGGCA GCACTGCATA ATTCTCTTAC TGTCATGCCA
     3121 TCCGTAAGAT GCTTTTCTGT GACTGGTGAG TACTCAACCA AGTCATTCTG AGAATAGTGT
     3181 ATGCGGCGAC CGAGTTGCTC TTGCCCGGCG TCAATACGGG ATAATACCGC GCCACATAGC
     3241 AGAACTTTAA AAGTGCTCAT CATTGGAAAA CGTTCTTCGG GGCGAAAACT CTCAAGGATC
     3301 TTACCGCTGT TGAGATCCAG TTCGATGTAA CCCACTCGTG CACCCAACTG ATCTTCAGCA
     3361 TCTTTTACTT TCACCAGCGT TTCTGGGTGA GCAAAAACAG GAAGGCAAAA TGCCGCAAAA
     3421 AAGGGAATAA GGGCGACACG GAAATGTTGA ATACTCATAC TCTTCCTTTT TCAATATTAT
     3481 TGAAGCATTT ATCAGGGTTA TTGTCTCATG AGCGGATACA TATTTGAATG TATTTAGAAA
     3541 AATAAACAAA TAGGGGTTCC GCGCACCTGC ACCAGTCAGT AAAACGACGG CCAGTAGTCA
     3601 AAAGCCTCCG ACCGGAGGCT TTTGACTTGG TTCAGGTGGA GTGGGAG
//