LOCUS       Exported     4109 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..4109
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      2146..2193
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     4041..4057
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      4058..4089
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     primer_bind     1..20
                     /label="dialout_for"
                     /note="Reverse dialout primer. Unique pair for each FreeGenes part."
     primer_bind     2029..2048
                     /label="dialout_rev"
                     /note="Forward dialout primer. Unique pair for each FreeGenes part."
     CDS             29..2014
                     /label="Moloney Murine Leukemia Virus (MMLV) Reverse Transcriptase (RNAse H deactivated by 3 mutations)"
                     /note=""
                     /translation="MTLNIEDEHRLHETSKEPDVSLGSTWLSDFPQAWAETGGMGLAVRQAPLIIPLKATSTPVSIKQYPMSQEARLGIKPHIQRLLDQGILVPCQSPWNTPLLPVKKPGTNDYRPVQDLREVNKRVEDIHPTVPNPYNLLSGLPPSHQWYTVLDLKDAFFCLRLHPTSQPLFAFEWRDPEMGISGQLTWTRLPQGFKNSPTLFDEALHRDLADFRIQHPDLILLQYVDDLLLAATSELDCQQGTRALLQTLGNLGYRASAKKAQICQKQVKYLGYLLKEGQRWLTEARKETVMGQPTPKTPRQLREFLGTAGFCRLWIPGFAEMAAPLYPLTKTGTLFNWGPDQQKAYQEIKQALLTAPALGLPDLTKPFELFVDEKQGYAKGVLTQKLGPWRRPVAYLSKKLDPVAAGWPPCLRMVAAIAVLTKDAGKLTMGQPLVILAPHAVEALVKQPPDRWLSNARMTHYQALLLDTDRVQFGPVVALNPATLLPLPEEGLQHNCLDILAEAHGTRPDLTDQPLPDADHTWYTGGSSLLQEGQRKAGAAVTTETEVIWAKALPAGTSAQRAQLIALTQALKMAEGKKLNVYTNSRYAFATAHIHGEIYRRRGLLTSEGKEIKNKDEILALLKALFLPKRLSIIHCPGHQKGHSAEARGNRMADQAARKAA*"
     primer_bind     complement(2198..2214)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(2281..2869)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(3060..3920)
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
     promoter        complement(3921..4025)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
     misc_feature    2049..2108
                     /label="FreeGenes Barcode"
                     /note="FreeGenes Barcode. Defined as I-SceI site flanked by 21 base pair random sequences."
ORIGIN
        1 AAAGGGGCCG TCAATATCAG GGTCTCTAAT GACTCTGAAC ATCGAAGATG AACATCGTCT
       61 GCACGAAACC AGCAAAGAAC CGGACGTTTC TCTGGGTTCA ACCTGGCTGT CTGATTTTCC
      121 GCAAGCTTGG GCCGAAACTG GCGGTATGGG CCTGGCCGTC CGTCAGGCAC CGCTGATCAT
      181 CCCACTGAAA GCGACCTCCA CCCCGGTGTC CATCAAACAG TATCCAATGT CCCAGGAAGC
      241 ACGTCTGGGC ATCAAGCCAC ATATCCAACG TCTGCTGGAT CAAGGTATCC TGGTACCGTG
      301 TCAATCGCCT TGGAACACTC CACTGCTGCC GGTGAAAAAA CCGGGCACTA ACGATTACCG
      361 TCCGGTACAG GACCTGCGTG AAGTTAACAA ACGTGTTGAA GATATTCATC CGACCGTCCC
      421 GAATCCGTAC AACCTCCTGT CCGGTCTGCC GCCGAGCCAT CAGTGGTACA CCGTGCTGGA
      481 CCTGAAGGAC GCATTCTTTT GCCTGCGTCT GCACCCGACC TCTCAGCCGC TGTTTGCGTT
      541 TGAATGGCGT GACCCGGAAA TGGGTATTTC TGGCCAGCTT ACATGGACCC GCCTGCCACA
      601 GGGTTTCAAA AACTCCCCGA CCCTTTTCGA CGAAGCCCTG CACCGTGACC TGGCAGATTT
      661 TCGCATTCAG CACCCGGACC TCATCCTGCT GCAATACGTG GACGATCTGC TGTTGGCAGC
      721 TACCAGCGAA CTGGATTGCC AGCAGGGTAC CCGTGCGCTG CTGCAAACCC TGGGCAACCT
      781 GGGTTACCGT GCTTCCGCGA AAAAGGCGCA GATTTGCCAA AAACAGGTCA AATATCTGGG
      841 TTACCTGCTG AAAGAAGGCC AGCGCTGGCT GACCGAGGCC CGCAAAGAAA CCGTGATGGG
      901 TCAACCGACC CCGAAAACCC CGCGTCAACT GCGCGAGTTT CTGGGTACCG CTGGCTTCTG
      961 CCGTCTGTGG ATTCCGGGCT TCGCTGAAAT GGCCGCGCCG CTGTATCCGT TAACCAAAAC
     1021 TGGTACTCTG TTCAACTGGG GTCCGGACCA GCAGAAAGCC TATCAGGAGA TTAAACAAGC
     1081 GCTGCTGACC GCACCGGCGC TGGGCCTGCC GGACCTGACC AAACCATTTG AACTGTTCGT
     1141 TGACGAGAAA CAAGGTTACG CTAAAGGTGT TCTTACCCAG AAACTGGGTC CGTGGCGCCG
     1201 TCCGGTAGCG TATCTGTCTA AAAAACTGGA CCCGGTTGCG GCTGGTTGGC CGCCGTGTCT
     1261 GCGTATGGTT GCTGCTATTG CCGTACTGAC TAAGGATGCG GGCAAACTGA CTATGGGCCA
     1321 GCCGCTGGTG ATCCTGGCTC CGCATGCGGT GGAGGCCCTG GTTAAACAGC CGCCGGATCG
     1381 CTGGCTGTCT AACGCGCGTA TGACACACTA CCAGGCTCTG CTGCTGGATA CCGACCGTGT
     1441 TCAGTTCGGC CCGGTCGTGG CCCTGAACCC GGCGACCCTG CTCCCGCTGC CGGAAGAAGG
     1501 TCTGCAACAC AATTGCCTGG ATATTCTGGC GGAAGCCCAT GGTACTCGTC CGGATCTGAC
     1561 CGACCAGCCT CTGCCGGATG CAGACCACAC CTGGTACACT GGTGGCAGCT CCCTCCTGCA
     1621 AGAGGGCCAG CGTAAGGCAG GCGCGGCTGT TACCACGGAA ACCGAAGTAA TCTGGGCTAA
     1681 AGCCCTGCCA GCAGGAACGT CAGCCCAACG CGCACAACTG ATTGCTCTGA CCCAGGCACT
     1741 GAAAATGGCG GAAGGTAAAA AGCTGAACGT TTACACTAAC TCCCGCTACG CATTCGCTAC
     1801 CGCCCACATC CATGGCGAAA TCTACAGACG TCGTGGTCTG CTGACCTCTG AAGGCAAAGA
     1861 AATCAAAAAC AAAGATGAGA TCCTGGCCCT GCTGAAAGCG CTGTTCCTGC CTAAACGTTT
     1921 ATCAATCATC CACTGTCCGG GCCACCAGAA GGGCCACTCC GCGGAAGCGC GCGGCAACCG
     1981 TATGGCAGAC CAGGCAGCAC GCAAAGCTGC CTGAAGAGCT TTGAGACCAG TATCTCAGCA
     2041 AGGGCAACCG ACGTTGTGAG AGCGCGGACT AGGGATAACA GGGTAATGCT TCCATCAGTG
     2101 AGAAGAGGCG CTGAGGTGTC AATCGTCGGA GCCGCTGAGC AATAACTAGC ATAACCCCTT
     2161 GGGGCCTCTA AACGGGTCTT GAGGGGTTTT TTGCATGGTC ATAGCTGTTT CCTGAGAGCT
     2221 TGGCAGGTGA TGACACACAT TAACAAATTT CGTGAGGAGT CTCCAGAAGA ATGCCATTAA
     2281 TTTCCATAGG CTCCGCCCCC CTGACGAGCA TCACAAAAAT CGACGCTCAA GTCAGAGGTG
     2341 GCGAAACCCG ACAGGACTAT AAAGATACCA GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG
     2401 CTCTCCTGTT CCGACCCTGC CGCTTACCGG ATACCTGTCC GCCTTTCTCC CTTCGGGAAG
     2461 CGTGGCGCTT TCTCATAGCT CACGCTGTAG GTATCTCAGT TCGGTGTAGG TCGTTCGCTC
     2521 CAAGCTGGGC TGTGTGCACG AACCCCCCGT TCAGCCCGAC CGCTGCGCCT TATCCGGTAA
     2581 CTATCGTCTT GAGTCCAACC CGGTAAGACA CGACTTATCG CCACTGGCAG CAGCCACTGG
     2641 TAACAGGATT AGCAGAGCGA GGTATGTAGG CGGTGCTACA GAGTTCTTGA AGTGGTGGCC
     2701 TAACTACGGC TACACTAGAA GAACAGTATT TGGTATCTGC GCTCTGCTGA AGCCAGTTAC
     2761 CTTCGGAAAA AGAGTTGGTA GCTCTTGATC CGGCAAACAA ACCACCGCTG GTAGCGGTGG
     2821 TTTTTTTGTT TGCAAGCAGC AGATTACGCG CAGAAAAAAA GGATCTCAAG AAGGCCTACT
     2881 ATTAGCAACA ACGATCCTTT GATCTTTTCT ACGGGGTCTG ACGCTCAGTG GAACGAAAAC
     2941 TCACGTTAAG GGATTTTGGT CATGAGATTA TCAAAAAGGA TCTTCACCTA GATCCTTTTA
     3001 AATTAAAAAT GAAGTTTTAA ATCAATCTAA AGTATATATG AGTAAACTTG GTCTGACAGT
     3061 TACCAATGCT TAATCAGTGA GGCACCTATC TCAGCGATCT GTCTATTTCG TTCATCCATA
     3121 GTTGCCTGAC TCCCCGTCGT GTAGATAACT ACGATACGGG AGGGCTTACC ATCTGGCCCC
     3181 AGTGCTGCAA TGATACCGCG AGAACCACGC TCACCGGCTC CAGATTTATC AGCAATAAAC
     3241 CAGCCAGCCG GAAGGGCCGA GCGCAGAAGT GGTCCTGCAA CTTTATCCGC CTCCATCCAG
     3301 TCTATTAATT GTTGCCGGGA AGCTAGAGTA AGTAGTTCGC CAGTTAATAG TTTGCGCAAC
     3361 GTTGTTGCCA TTGCTACAGG CATCGTGGTG TCACGCTCGT CGTTTGGTAT GGCTTCATTC
     3421 AGCTCCGGTT CCCAACGATC AAGGCGAGTT ACATGATCCC CCATGTTGTG CAAAAAAGCG
     3481 GTTAGCTCCT TCGGTCCTCC GATCGTTGTC AGAAGTAAGT TGGCCGCAGT GTTATCACTC
     3541 ATGGTTATGG CAGCACTGCA TAATTCTCTT ACTGTCATGC CATCCGTAAG ATGCTTTTCT
     3601 GTGACTGGTG AGTACTCAAC CAAGTCATTC TGAGAATAGT GTATGCGGCG ACCGAGTTGC
     3661 TCTTGCCCGG CGTCAATACG GGATAATACC GCGCCACATA GCAGAACTTT AAAAGTGCTC
     3721 ATCATTGGAA AACGTTCTTC GGGGCGAAAA CTCTCAAGGA TCTTACCGCT GTTGAGATCC
     3781 AGTTCGATGT AACCCACTCG TGCACCCAAC TGATCTTCAG CATCTTTTAC TTTCACCAGC
     3841 GTTTCTGGGT GAGCAAAAAC AGGAAGGCAA AATGCCGCAA AAAAGGGAAT AAGGGCGACA
     3901 CGGAAATGTT GAATACTCAT ACTCTTCCTT TTTCAATATT ATTGAAGCAT TTATCAGGGT
     3961 TATTGTCTCA TGAGCGGATA CATATTTGAA TGTATTTAGA AAAATAAACA AATAGGGGTT
     4021 CCGCGCACCT GCACCAGTCA GTAAAACGAC GGCCAGTAGT CAAAAGCCTC CGACCGGAGG
     4081 CTTTTGACTT GGTTCAGGTG GAGTGGGAG
//